scholarly journals Production of Xyloligosaccharides from Rice Straw by Microwave-assisted Enzymatic Hydrolysis and Evaluation of Their Prebiotic Properties

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Alisa Pattarapisitporn ◽  
Nonglak Thiangthong ◽  
Pakorn Inthajak ◽  
Pannapapol Jaichakan ◽  
Wantana Panpa ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Sugar Content ◽  
Lactobacillus Brevis ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Microwave Pretreatment ◽  
Pretreatment Time ◽  
Layer Chromatography

Rice straw (RS) is a by-product from rice production process. It is rich in cellulose, hemicellulose and lignin. RS hemicellulose mainly composes of arabinoxylan (AX). This research aimed to investigate the potential of microwave-pretreatment in AX extraction from RS and substrate to produce xylooligosaccharides (XOS) via enzymatic hydrolysis. The extractive-free RS was pretreated by microwave process at 160°C for 5-15 min, then the AX was extracted with 4% sodium hydroxide. The total sugar and reducing sugar content of AX exhibited that increasing microwave-pretreatment time increased the yield of AX. The highest AX content was found at 160°C for 10 min as 7.73%, reducing sugar content of 11.89 mg/g, and total sugar of 165.85 mg/g. The crude AX obtained by microwave-pretreatment was then used as a substrate for XOS production by two commercial xylanases of Pentopan mono BG (BG) and Ultraflo Max (UM), at the enzyme concentration of 50-300 U/g AX (50°C, pH 6.0) for 24 h.The reducing sugar content and sugar profiles were monitored by DNS assay,and thin layer chromatography (TLC) which revealed that BG 50 U/g at 12 h andUM 50 U/g at 24 h showed the promising reducing sugar of 16.4 and 25.44 mg/g,respectively. The composition of XOS derived from RS (RS-XOS) prepared by BGwas xylobiose (X2), xylotriose (X3), xylotretraose (X4), and xylopentaose (X5)while by UM was xylobiose (X2), xylotriose (X3) and xylotretraose (X5). Moreover,XOS produced by BG contained very low amount of xylose (X1). In addition, theRS-XOS could the growth of Lactobacillus brevis greater than commercial XOS.

scholarly journals Microwave-assisted Enzymatic Hydrolysis to Produce Xylooligosaccharides from Rice Husk Alkali- soluble Arabinoxylan

2021 ◽  
Author(s):  
Wannaporn Klangpetch ◽  
Alisa Pattarapisitporn ◽  
Suphat Phongthai ◽  
Pannapapol Jaichakan
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sodium Hydroxide ◽  
Microwave Heating ◽  
Rice Husk ◽  
Sugar Content ◽  
Microwave Treatment ◽  
Heating Process ◽  
Microwave Pretreatment ◽  
Microwave Assisted ◽  
Pretreatment Time

Abstract This study aimed to produce xylooligosaccharide (XOS) and arabino-xylooligosaccharide (AXOS) from rice husk (RH) using microwave treatment combined with enzymatic hydrolysis and evaluate their prebiotic properties. The RH was pretreated by a microwave heating process at 140, 160 and 180°C for 5, 10, and 15 min to obtain crude arabinoxylan (AX). The results emphasized that increasing microwave pretreatment time increased sugar content. The crude AX was then extracted with 2% (w/v) sodium hydroxide at 25°C for 24 h. The obtained AX was then used as a substrate for XOS production by commercial xylanases. The results showed that the oligosaccharide produced by Pentopan Mono BG and Ultraflo Max provided xylobiose and xylotriose as the main products. Interestingly, AXOS was also present in the oligosaccharide product. Furthermore, the oligosaccharides obtained were able to promote the growth of Lactobacillus spp. and to resist degradation more than 70% after exposure to simulated human digestion.

Optimization of Microwave Pretreatment without a Reagent of Pineapple Peel

2014 ◽  
Vol 941-944 ◽  
pp. 1056-1059
Author(s):  
Phubet Pongsuwan ◽  
Sininart Chongkhong
Keyword(s):  
Response Surface Methodology ◽  
Energy Consumption ◽  
Sugar Content ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Sugar Production ◽  
Microwave Pretreatment ◽  
Pineapple Peel ◽  
Time And Energy ◽  
Water Ratio

The microwave pretreatment of pineapple peel for ethanol production was optimized by RSM (Response surface methodology). Three variables, which were studied, were peel to water ratio (100-160 g/L), microwave power (90-900 watt) and time (5-20 min). The optimal condition for reducing sugar production was 132 g/L peel to water ratio, 90 watt for 12 min that provide the 10.4 g/L of highest content. For total sugar production was 160 g/L ratio, 90 watt for 20 min that gave the optimum content of 579.9 g/L. This microwave pretreatment without a reagent could achieve the satisfied sugar content for ethanol fermentation that led to save time and energy consumption.

scholarly journals Microwave-assisted enzymatic hydrolysis to produce xylooligosaccharides from rice husk alkali-soluble arabinoxylan

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Wannaporn Klangpetch ◽  
Alisa Pattarapisitporn ◽  
Suphat Phongthai ◽  
Niramon Utama-ang ◽  
Thunnop Laokuldilok ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sodium Hydroxide ◽  
Rice Husk ◽  
Sugar Content ◽  
Microwave Treatment ◽  
Microwave Pretreatment ◽  
Microwave Assisted ◽  
Pretreatment Time

AbstractThe prebiotic properties of xylooligosaccharides (XOS) and arabino-xylooligosaccharides (AXOS) produced from rice husk (RH) using microwave treatment combined with enzymatic hydrolysis were evaluated. The RH was subjected to microwave pretreatment at 140, 160 and 180 °C for 5, 10 and 15 min to obtain crude arabinoxylan (AX). Increasing microwave pretreatment time increased sugar content. Crude AX was extracted with 2% (w/v) sodium hydroxide at 25 °C for 24 h and used as a substrate for XOS production by commercial xylanases. Results showed that oligosaccharides produced by Pentopan Mono BG and Ultraflo Max provided xylobiose and xylotriose as the main products. AXOS was also present in the oligosaccharides that promoted growth of Lactobacillus spp. and resisted degradation by over 70% after exposure to simulated human digestion.

scholarly journals Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Vera BARLIANTI ◽  
Deliana DAHNUM ◽  
. MURYANTO ◽  
Eka TRIWAHYUNI ◽  
Yosi ARISTIAWAN ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Volume Ratio ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Economic Feasibility ◽  
First Order ◽  
Empty Fruit Bunches ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    

PRETREATMENT OF MILLET HUSK USING ALKALINE HYDROGEN PEROXIDE TO ENHANCE ENZYMATIC HYDROLYSIS FOR REDUCING SUGAR PRODUCTION

2021 ◽  
Vol 5 (2) ◽  
pp. 289-294
Author(s):  
Zeenat Ibrahim Saulawa ◽  
Lawal Nura ◽  
Muntari Bala ◽  
Abdullahi A. Iman
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Hydrolysis ◽  
Reducing Sugars ◽  
Reducing Sugar ◽  
Sugar Concentration ◽  
Sugar Production ◽  
Alkaline Hydrogen Peroxide ◽  
Pretreatment Time ◽  
Pretreatment Temperature ◽  
Reducing Sugar Concentration

The effectiveness of alkaline hydrogen peroxide as a suitable choice of pretreatment for the conversion of millet husk to reducing sugars using cellulase enzyme for hydrolysis and subsequent ethanol production was determined. The effects of three variables on reducing sugar production from millet husk were determined using one factor at a time (OFAT) method namely; peroxide concentration, pretreatment time and pretreatment temperature. From the results, it was observed that a significant (P<0.05) amount of reducing sugars were lost during pretreatment of millet husk. The untreated group which was only physically pretreated (milled) however yielded a significantly higher (P<0.05) reducing sugar concentration of 10.67mg/ml after enzymatic hydrolysis while the highest reducing sugar concentration of 4.82mg/ml was obtained using 0.375%v/v peroxide concentration for 60minutes at 250C. Therefore, pretreatment of biomass with alkaline hydrogen peroxide may be more suitable for feedstock with high lignin contents than millet husk.

Comparison of Bacterial and Yeast Ethanol Fermentation Yield from Rice Straw

2011 ◽  
Vol 347-353 ◽  
pp. 2541-2544
Author(s):  
Benjarat Laobussararak ◽  
Warawut Chulalaksananukul ◽  
Orathai Chavalparit
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Zymomonas Mobilis ◽  
Ethanol Yield ◽  
Sugar Content ◽  
Alkali Pretreatment ◽  
Yeast Saccharomyces Cerevisiae ◽  
Pretreatment Condition ◽  
Theoretical Yield

This study was to investigate the fermentation of rice straw using various microorganisms, i.e., the bacterium Zymomonas mobilis, a distillery yeast Saccharomyces cerevisiae and a co-culture of Zymomonas mobilis and Saccharomyces cerevisiae. Rice straw was pretreated with alkaline and followed by enzymatic hydrolysis using cellulase before fermentation by the bacterium and a distillery yeast. Results show that alkali pretreatment is appropriate for rice straw since this pretreatment condition can produce the maximum cellulose of 88.96% and reducing sugar content of 9.18 g/l. Furthermore, the ethanol yield after enzymatic hydrolysis (expressed as % theoretical yield) was 15.94-19.73% for the bacterium, 20.48-35.70% for yeast and 21.56-29.89% for co-culture. Therefore, the distillery yeast was a suitable microorganism for ethanol production from rice straw.

scholarly journals PENGARUH SUHU DAN JENIS ASAM PADA HIDROLISIS PATI UBI TALAS (Colocasia esculenta L. Schott) TERHADAP KARAKTERISTIK GLUKOSA

2018 ◽  
Vol 6 (4) ◽  
pp. 307
Author(s):  
Ni Kadek Ariani Dewi ◽  
Amna Hartiati ◽  
Bambang Admadi Harsojuwono
Keyword(s):  
Block Design ◽  
Sugar Content ◽  
Total Dissolved Solids ◽  
Temperature Treatment ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Effect Of Temperature ◽  
Dissolved Solids ◽  
Randomized Block Design ◽  
Acid Type

The purpose of this reseach was 1) to determine the effect of temperature and acid type on hydrolysis of taro yam starch on the characteristics of glucose, 2) to determine the exact temperature and type of acid in taro starch hydrolysis which produced the best glucose characteristics. This reseach uses Randomized Block Design of factorial pattern.. The first factor is the hydrolysis temperature of taro yam starch at temperatures of 80°C, 90°C and 100°C. The second factor is the acids type of HNO3, H2SO4 and HCl, each with 7% acid concentration. Each treatment is grouped into 2 based on the implementation time. The variables observed were reduction in sugar content, total sugar, dekstrose equivalent (DE), total dissolved solids and clarity. The results showed that the temperature treatment and acid type had a very significant effect (P<0,01) on reducing sugar content, total sugar and hydrolyzed dectrose equivalent (DE) values. Temperature treatment has a significant effect on total dissolved solids and does not significantly affect clarity. The treatment of acid types has significant effect on total dissolved solids and clarity. Temperature treatment and type of acid interact with reducing sugar content, total sugar, and DE values, do not interact with total dissolved solids and clarity. The highest yield was obtained by reducing sugar content of 3,06%, total sugar 5,64% and DE value of 54,24% in HCl at 100°C. The total dissolved solids in H2SO4 and HCl at 100°C were 5,15°Brix and the clarity of HCl at 100°C was 0,02. The best glucose characteristics at HCl was 90°C which was not significantly different from HCl at 100°C. Keywords: sugar, hydrolysis, taro yam, starch, acid, glucose

scholarly journals Reducing and Total Sugar Content in Onion During Storage in the Republic of Macedonia

2017 ◽  
Vol 17 (1) ◽  
pp. 37 ◽  
Author(s):  
Zvezda Bogevska ◽  
Rukie Agic ◽  
Gordana Popsimonova ◽  
Margarita Davitkovska ◽  
Igor Iljovski
Keyword(s):  
Chemical Analysis ◽  
Sugar Content ◽  
Storage Period ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Winter Period ◽  
Total Sugar Content ◽  
Republic Of Macedonia ◽  
Cold Room ◽  
The Republic

In Macedonia, sweet onion landrace ‘buchinska arshlama’ is traditionally stored during winter period from October until April. The objective of this research was to determine the content of reducing sugar and total sugar in onion bulbs during storage period in a traditional way and in cold rooms. Every month, samples were taken for chemical analysis. The results were statistically processed and tested with t-test. The average reducing sugar content in onion stored in a traditional way was 4.8%, while the average reducing sugar content in onion stored in cold rooms was 4.6%. The average total sugar content in onion stored in a traditional way was 6.0%, while the average total sugar content in onion stored in a cold room was 5.6%. These results showed that onion `buchinska arshlama` can be successfully stored in a traditional way until the beginning of February or longer in cold rooms.

scholarly journals Evaluation of sugar and free amino acid during fermentation of ogi from maize, acha and sorghum

2021 ◽  
Vol 4 (2) ◽  
pp. 111
Author(s):  
Adekunbi Adetola Malomo ◽  
Babatunde Olawoye ◽  
Abiola Folakemi Olaniran ◽  
Omowumi Ibipeju Olaniyi ◽  
Adedoyin Adedayo ◽  
...  
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Sugar Content ◽  
Amino Acid Content ◽  
Principal Component ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Diabetic Patients ◽  
Fermentation Time

This research investigated effect of fermentation time and cereal type on the total reducing sugar (TRS), total sugar (TSS), and total free amino acid (TFA) during the production of ogi. The result showed that TFA generally increased with increase in fermentation time (7.916 – 17.596 mg/g). Maize, acha and sorghum ogi had the lowest total reducing sugar (TRS) at 0 h (16.927glucose mg/g), 12 h (16.655 glucose mg/g) and 48 h (18.212 glucose mg/g) respectively and TSS was lowest in acha ogi from 12 h to 48 h (33.191 - 34.370 glucose mg/g). Principal component analysis and Agglomerative hierarchical clustering were used to evaluate the variability in sugar and amino acid contents and ranked the contributions of the variables. The factors were divided into four principal components with cumulative variance contribution rate of 87.47%. The result showed that acha and sorghum ogi had lower sugar content than maize ogi during fermentation. This research suggested that maize, acha and sorghum can be used in the production of cereal based ogi for weaning food at 48 h due to high free amino acid content, and also advanced the use of acha in production of ogi for diabetic patients due to its low total sugar content.

scholarly journals Improving quality of dragon fruit (hylocereus costaricensis) syrup by processing with double jacket vacuum evaporator

2021 ◽  
Vol 924 (1) ◽  
pp. 012012
Author(s):  
B Susilo ◽  
S M Sutan ◽  
Y Hendrawan ◽  
R Damayanti
Keyword(s):  
Vitamin C ◽  
Moisture Content ◽  
Sugar Content ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Evaporation Process ◽  
Nutritional Content ◽  
Total Sugar Content ◽  
Dragon Fruit

Abstract Red dragon fruit (Hylocereus costaricensis) is a famous fruit because of its high nutritional content. Although the product diversification of red dragon fruit is quite a lot, however, this product has limited information as a syrup product. The evaporation process for making syrup at the traditional level is usually carried out at high temperatures which results in the high destruction of nutritional content and reduces the quality of syrup. The study of the evaporation process at low temperature and low pressure using a double jacket vacuum evaporator for manufacturing red dragon fruit syrup was conducted in this research in order to improve product quality. The aims of this study are to evaluate the effect of temperature process and vacuum pressure on the quality of red dragon fruit (H.costaricensis) syrup using a double jacket vacuum evaporator and to know the optimum temperature process and vacuum pressure to producing red dragon fruit sirup with higher content of vitamin C. The double jacket evaporator operates at a pressure lower than atmospheric pressure so that there were 9 treatment combinations. The result showed the temperature gave a highly significant effect, especially on vitamin C. It also gave on moisture content and reducing sugar. The temperature gave no significant effect on total sugar content. Whereas, the pressure gave a significant on moisture content, but not gave a significant on vitamin C, reducing sugar content and total sugar content. However, the best treatment for optimum vitamin C is 50 0C/-60 cmHg with 8.75 mg/100 ml.

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