Effects of growth stage on enzymatic saccharification and simultaneous saccharification and fermentation of bamboo shoots for bioethanol production

Simultaneous saccharification and fermentation (SSF) is capable of performing enzymatic saccharification and fermentation for biofuel production in a single vessel. Thus, SSF has several advantages such as simplifying the manufacturing process, operating easily, and reducing energy input. Here, we describe the application of Pichia kudriavzevii NBRC1279 and NBRC1664 to SSF for bioethanol production. When each strain was incubated for 144 h at 35 °C with Japanese cedar particles, the highest ethanol concentrations were reached 21.9 ± 0.50 g/L and 23.8 ± 3.9 g/L, respectively. In addition, 21.6 ± 0.29 g/L and 21.3 ± 0.21 g/L of bioethanol were produced from Japanese eucalyptus particles when each strain was incubated for 144 h at 30 °C. Although previous methods require pretreatment of the source material, our method does not require pretreatment, which is an advantage for industrial use. To elucidate the different characteristics of the strains, we performed genome sequencing and genome comparison. Based on the results of the eggNOG categories and the resulting Venn diagram, the functional abilities of both strains were similar. However, strain NBRC1279 showed five retrotransposon protein genes in the draft genome sequence, which indicated that the stress tolerance of both strains is slightly different.

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Bioethanol production from lignocellulosic feedstock using aqueous ammonia pretreatment and simultaneous saccharification and fermentation (SSF): process development and optimization

10.31274/etd-180810-551 ◽

2010 ◽

Author(s):

Xuan Li

Keyword(s):

Simultaneous Saccharification And Fermentation ◽

Process Development ◽

Aqueous Ammonia ◽

Bioethanol Production ◽

Lignocellulosic Feedstock ◽

Ammonia Pretreatment ◽

Ssf Process ◽

Simultaneous Saccharification

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Production and optimization of cellulase from agricultural waste and its application in bioethanol production by simultaneous saccharification and fermentation

Management of Environmental Quality An International Journal ◽

10.1108/meq-07-2015-0128 ◽

2016 ◽

Vol 27 (1) ◽

pp. 22-35 ◽

Cited By ~ 2

Author(s):

Elsa Cherian ◽

M. Dharmendira Kumar ◽

G. Baskar

Keyword(s):

Design Methodology ◽

Simultaneous Saccharification And Fermentation ◽

Agricultural Waste ◽

Cellulase Production ◽

Reducing Sugar ◽

Bioethanol Production ◽

Corn Cob ◽

Content Type ◽

Optimized Conditions ◽

Simultaneous Saccharification

Purpose – The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast. Design/methodology/approach – Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation. Findings – Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production. Originality/value – The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.

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