scholarly journals Application of Pichia kudriavzevii NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol Production

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 83
Author(s):  
Hironaga Akita ◽  
Tetsuya Goshima ◽  
Toshihiro Suzuki ◽  
Yuya Itoiri ◽  
Zen-ichiro Kimura ◽  
...  
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Draft Genome ◽  
Enzymatic Saccharification ◽  
Japanese Cedar ◽  
Biofuel Production ◽  
Venn Diagram ◽  
Genome Comparison ◽  
Bioethanol Production ◽  
Pichia Kudriavzevii ◽  
Simultaneous Saccharification

Simultaneous saccharification and fermentation (SSF) is capable of performing enzymatic saccharification and fermentation for biofuel production in a single vessel. Thus, SSF has several advantages such as simplifying the manufacturing process, operating easily, and reducing energy input. Here, we describe the application of Pichia kudriavzevii NBRC1279 and NBRC1664 to SSF for bioethanol production. When each strain was incubated for 144 h at 35 °C with Japanese cedar particles, the highest ethanol concentrations were reached 21.9 ± 0.50 g/L and 23.8 ± 3.9 g/L, respectively. In addition, 21.6 ± 0.29 g/L and 21.3 ± 0.21 g/L of bioethanol were produced from Japanese eucalyptus particles when each strain was incubated for 144 h at 30 °C. Although previous methods require pretreatment of the source material, our method does not require pretreatment, which is an advantage for industrial use. To elucidate the different characteristics of the strains, we performed genome sequencing and genome comparison. Based on the results of the eggNOG categories and the resulting Venn diagram, the functional abilities of both strains were similar. However, strain NBRC1279 showed five retrotransposon protein genes in the draft genome sequence, which indicated that the stress tolerance of both strains is slightly different.

scholarly journals Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Shannon M. Hoffman ◽  
Maria Alvarez ◽  
Gilad Alfassi ◽  
Dmitry M. Rein ◽  
Sergio Garcia-Echauri ◽  
...  
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Agricultural Waste ◽  
Biofuel Production ◽  
Cellulosic Biofuels ◽  
Processing Times ◽  
Cellulosic Biofuel ◽  
Thermotolerant Yeasts ◽  
Rapid Hydrolysis ◽  
Dedicated Energy Crops ◽  
Simultaneous Saccharification

Abstract Background Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are necessary. To date, slow and incomplete saccharification as well as high enzyme costs have hindered the economic viability of cellulosic biofuels, and while approaches like simultaneous saccharification and fermentation (SSF) and the use of thermotolerant microorganisms can enhance production, further improvements are needed. Cellulosic emulsions have been shown to enhance saccharification by increasing enzyme contact with cellulose fibers. In this study, we use these emulsions to develop an emulsified SSF (eSSF) process for rapid and efficient cellulosic biofuel production and make a direct three-way comparison of ethanol production between S. cerevisiae, O. polymorpha, and K. marxianus in glucose and cellulosic media at different temperatures. Results In this work, we show that cellulosic emulsions hydrolyze rapidly at temperatures tolerable to yeast, reaching up to 40-fold higher conversion in the first hour compared to microcrystalline cellulose (MCC). To evaluate suitable conditions for the eSSF process, we explored the upper temperature limits for the thermotolerant yeasts Kluyveromyces marxianus and Ogataea polymorpha, as well as Saccharomyces cerevisiae, and observed robust fermentation at up to 46, 50, and 42 °C for each yeast, respectively. We show that the eSSF process reaches high ethanol titers in short processing times, and produces close to theoretical yields at temperatures as low as 30 °C. Finally, we demonstrate the transferability of the eSSF technology to other products by producing the advanced biofuel isobutanol in a light-controlled eSSF using optogenetic regulators, resulting in up to fourfold higher titers relative to MCC SSF. Conclusions The eSSF process addresses the main challenges of cellulosic biofuel production by increasing saccharification rate at temperatures tolerable to yeast. The rapid hydrolysis of these emulsions at low temperatures permits fermentation using non-thermotolerant yeasts, short processing times, low enzyme loads, and makes it possible to extend the process to chemicals other than ethanol, such as isobutanol. This transferability establishes the eSSF process as a platform for the sustainable production of biofuels and chemicals as a whole.

Production and optimization of cellulase from agricultural waste and its application in bioethanol production by simultaneous saccharification and fermentation

2016 ◽  
Vol 27 (1) ◽  
pp. 22-35 ◽  
Author(s):  
Elsa Cherian ◽  
M. Dharmendira Kumar ◽  
G. Baskar
Keyword(s):  
Design Methodology ◽  
Simultaneous Saccharification And Fermentation ◽  
Agricultural Waste ◽  
Cellulase Production ◽  
Reducing Sugar ◽  
Bioethanol Production ◽  
Corn Cob ◽  
Content Type ◽  
Optimized Conditions ◽  
Simultaneous Saccharification

Purpose – The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast. Design/methodology/approach – Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation. Findings – Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production. Originality/value – The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.

scholarly journals Diverse Banana Pseudostems and Rachis Are Distinctive for Edible Carbohydrates and Lignocellulose Saccharification towards High Bioethanol Production under Chemical and Liquid Hot Water Pretreatments

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3870
Author(s):  
Jingyang Li ◽  
Fei Liu ◽  
Hua Yu ◽  
Yuqi Li ◽  
Shiguang Zhou ◽  
...  
Keyword(s):  
Soluble Sugars ◽  
Enzymatic Saccharification ◽  
Hot Water ◽  
Biofuel Production ◽  
Bioenergy Crops ◽  
Bioethanol Production ◽  
Factors Affecting ◽  
Liquid Hot Water ◽  
Biomass Processing ◽  
Banana Crops

Banana is a major fruit crop throughout the world with abundant lignocellulose in the pseudostem and rachis residues for biofuel production. In this study, we collected a total of 11 pseudostems and rachis samples that were originally derived from different genetic types and ecological locations of banana crops and then examined largely varied edible carbohydrates (soluble sugars, starch) and lignocellulose compositions. By performing chemical (H2SO4, NaOH) and liquid hot water (LHW) pretreatments, we also found a remarkable variation in biomass enzymatic saccharification and bioethanol production among all banana samples examined. Consequently, this study identified a desirable banana (Refen1, subgroup Pisang Awak) crop containing large amounts of edible carbohydrates and completely digestible lignocellulose, which could be combined to achieve the highest bioethanol yields of 31–38% (% dry matter), compared with previously reported ones in other bioenergy crops. Chemical analysis further indicated that the cellulose CrI and lignin G-monomer should be two major recalcitrant factors affecting biomass enzymatic saccharification in banana pseudostems and rachis. Therefore, this study not only examined rich edible carbohydrates for food in the banana pseudostems but also detected digestible lignocellulose for bioethanol production in rachis tissue, providing a strategy applicable for genetic breeding and biomass processing in banana crops.

Increase in bioethanol production yield from triticale by simultaneous saccharification and fermentation with application of ultrasound

2011 ◽  
Vol 87 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Dušanka J. Pejin ◽  
Ljiljana V. Mojović ◽  
Jelena D. Pejin ◽  
Olgica S. Grujić ◽  
Siniša L. Markov ◽  
...  
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Production Yield ◽  
Bioethanol Production ◽  
Simultaneous Saccharification
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