Fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enhancing enzymatic saccharification and hydrogen production

2012 ◽  
Vol 114 ◽  
pp. 365-369 ◽  
Author(s):  
Lei Zhao ◽  
Guang-Li Cao ◽  
Ai-Jie Wang ◽  
Hong-Yu Ren ◽  
De Dong ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Phanerochaete Chrysosporium ◽  
Enzymatic Saccharification ◽  
Fungal Pretreatment

Pretreatment of lignocellulosic material with fungi capable of higher lignin degradation and lower carbohydrate degradation improves substrate acid hydrolysis and the eventual conversion to ethanol

2008 ◽  
Vol 54 (4) ◽  
pp. 305-313 ◽  
Author(s):  
Sarika Kuhar ◽  
Lavanya M. Nair ◽  
Ramesh Chander Kuhad
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Degradation ◽  
Ethanol Yield ◽  
Fungal Isolate ◽  
Fermentation Inhibitors ◽  
Pycnoporus Cinnabarinus ◽  
Acid Hydrolysate ◽  
Carbohydrate Degradation ◽  
Fungal Pretreatment ◽  
Acid Load

Phanerochaete chrysosporium , Pycnoporus cinnabarinus ,and fungal isolates RCK-1 and RCK-3 were tested for their lignin degradation abilities when grown on wheat straw (WS) and Prosopis juliflora (PJ) under solid-state cultivation conditions. Fungal isolate RCK-1 degraded more lignin in WS (12.26% and 22.64%) and PJ (19.30% and 21.97%) and less holocellulose in WS (6.27% and 9.39%) and PJ (3.01% and 4.58%) after 10 and 20 days, respectively, than other fungi tested. Phanerochaete chrysosporium caused higher substrate mass loss and degraded more of holocellulosic content (WS: 55.67%; PJ: 48.89%) than lignin (WS: 18.89%; PJ: 20.20%) after 20 days. The fungal pretreatment of WS and PJ with a high-lignin-degrading and low-holocellulose-degrading fungus (fungal isolate RCK-1) for 10 days resulted in (i) reduction in acid load for hydrolysis of structural polysaccharides (from 3.5% to 2.5% in WS and from 4.5% to 2.5% in PJ), (ii) an increase in the release of fermentable sugars (from 30.27 to 40.82 g·L–1in WS and from 18.18 to 26.00 g·L–1in PJ), and (iii) a reduction in fermentation inhibitors (total phenolics) in acid hydrolysate of WS (from 1.31 to 0.63 g·L–1) and PJ (from 2.05 to 0.80 g·L–1). Ethanol yield and volumetric productivity from RCK-1-treated WS (0.48 g·g–1and 0.54 g·L–1·h–1, respectively) and PJ (0.46 g·g–1and 0.33 g·L–1·h–1, respectively) were higher than untreated WS (0.36 g·g–1and 0.30 g·L–1·h–1, respectively) and untreated PJ (0.42 g·g–1and 0.21 g·L–1·h–1, respectively).

scholarly journals Biological pretreatment of wheat straw: Effect of fungal culturing on enzymatic hydrolysis of carbohydrate polymers

2021 ◽  
Author(s):  
Aleksandar Knežević ◽  
Ivana Đokić ◽  
Tomislav Tosti ◽  
Slađana Popović ◽  
Dušanka Milojković-Opsenica ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Lignin Degradation ◽  
Enzymatic Saccharification ◽  
White Rot Fungi ◽  
Fungal Species ◽  
White Rot ◽  
Irpex Lacteus ◽  
Fungal Pretreatment ◽  
Hydrolysis Of

Abstract The aim of the study was comparative analysis of degradation of wheat straw lignin by white-rot fungi and its implications on the efficiency of enzymatic hydrolysis of holocellulose. Cyclocybe cylindracea, Ganoderma resinaceum, Irpex lacteus, Pleurotus ostreatus and Trametes versicolor were the species studied. Peroxidases were predominantly responsible for lignin degradation even though high laccase activities were detected, except in the case of Irpex lacteus where laccase activity was not detected. Studied fungal species showed various ability to degrade lignin in wheat straw which further affected release of reducing sugars during enzymatic saccharification. The highest rate of lignin degradation was noticed in sample pretreated with Irpex lacteus (50.9 ± 4.1%). Among all tested species only Ganoderma resinaceum was suitable lignin degrader with the 2-fold higher hydrolysis yield (51.1 ± 4.7%) than in the control, and could have significant biotechnological application due to lower cellulose loss. A key mechanism of carbohydrate component convertibility enhancement was lignin removal in the biomass. Long time consumption, the low sugar yields and unpredictable fungal response still remain the challenge of the fungal pretreatment process.

scholarly journals Enhancing the Enzymatic Saccharification of Grain Stillage by Combining Microwave-Assisted Hydrothermal Irradiation and Fungal Pretreatment

ACS Omega ◽  
2020 ◽  
Vol 5 (22) ◽  
pp. 12603-12614
Author(s):  
Haiwei Ren ◽  
Wenli Sun ◽  
Zhiye Wang ◽  
Shanfei Fu ◽  
Yi Zheng ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Microwave Assisted ◽  
Fungal Pretreatment

scholarly journals Enhanced biohydrogen production from nutrient-free anaerobic fermentation medium with edible fungal pretreated rice straw

RSC Advances ◽  
2018 ◽  
Vol 8 (41) ◽  
pp. 22924-22930 ◽  
Author(s):  
Tao Sheng ◽  
Lei Zhao ◽  
Lingfang Gao ◽  
Wenzong Liu ◽  
Guofeng Wu ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Rice Straw ◽  
Anaerobic Fermentation ◽  
Fermentation Medium ◽  
Biohydrogen Production ◽  
Biological Hydrogen Production ◽  
Fungal Pretreatment

An edible fungal pretreatment of rice straw was proposed for enhanced hydrogen production while reducing the chemical cost for traditional biological hydrogen production from lignocellulose.

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