scholarly journals FTIR as an easy and fast analytical approach to follow up microbial growth during fungal pretreatment of poplar wood with Phanerochaete chrysosporium

2018 ◽  
Vol 145 ◽  
pp. 82-86 ◽  
Author(s):  
I. Cornet ◽  
N. Wittner ◽  
G. Tofani ◽  
S. Tavernier
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Analytical Approach ◽  
Microbial Growth ◽  
Poplar Wood ◽  
Fungal Pretreatment

Pretreatment of lignocellulosic material with fungi capable of higher lignin degradation and lower carbohydrate degradation improves substrate acid hydrolysis and the eventual conversion to ethanol

2008 ◽  
Vol 54 (4) ◽  
pp. 305-313 ◽  
Author(s):  
Sarika Kuhar ◽  
Lavanya M. Nair ◽  
Ramesh Chander Kuhad
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Degradation ◽  
Ethanol Yield ◽  
Fungal Isolate ◽  
Fermentation Inhibitors ◽  
Pycnoporus Cinnabarinus ◽  
Acid Hydrolysate ◽  
Carbohydrate Degradation ◽  
Fungal Pretreatment ◽  
Acid Load

Phanerochaete chrysosporium , Pycnoporus cinnabarinus ,and fungal isolates RCK-1 and RCK-3 were tested for their lignin degradation abilities when grown on wheat straw (WS) and Prosopis juliflora (PJ) under solid-state cultivation conditions. Fungal isolate RCK-1 degraded more lignin in WS (12.26% and 22.64%) and PJ (19.30% and 21.97%) and less holocellulose in WS (6.27% and 9.39%) and PJ (3.01% and 4.58%) after 10 and 20 days, respectively, than other fungi tested. Phanerochaete chrysosporium caused higher substrate mass loss and degraded more of holocellulosic content (WS: 55.67%; PJ: 48.89%) than lignin (WS: 18.89%; PJ: 20.20%) after 20 days. The fungal pretreatment of WS and PJ with a high-lignin-degrading and low-holocellulose-degrading fungus (fungal isolate RCK-1) for 10 days resulted in (i) reduction in acid load for hydrolysis of structural polysaccharides (from 3.5% to 2.5% in WS and from 4.5% to 2.5% in PJ), (ii) an increase in the release of fermentable sugars (from 30.27 to 40.82 g·L–1in WS and from 18.18 to 26.00 g·L–1in PJ), and (iii) a reduction in fermentation inhibitors (total phenolics) in acid hydrolysate of WS (from 1.31 to 0.63 g·L–1) and PJ (from 2.05 to 0.80 g·L–1). Ethanol yield and volumetric productivity from RCK-1-treated WS (0.48 g·g–1and 0.54 g·L–1·h–1, respectively) and PJ (0.46 g·g–1and 0.33 g·L–1·h–1, respectively) were higher than untreated WS (0.36 g·g–1and 0.30 g·L–1·h–1, respectively) and untreated PJ (0.42 g·g–1and 0.21 g·L–1·h–1, respectively).

scholarly journals The Preoperative Microbial Detection is No Prerequisite for the Indication of Septic Revision in Cases of Suspected Periprosthetic Joint Infection

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Daniel Karczewski ◽  
Tobias Winkler ◽  
Carsten Perka ◽  
Michael Müller
Keyword(s):  
Preoperative Diagnosis ◽  
Microbial Growth ◽  
Joint Infection ◽  
Clinical Signs ◽  
Diagnostic Algorithm ◽  
Important Criterion ◽  
Loss Of Function ◽  
Joint Aspiration ◽  
Septic Revision

Aim of This Study. Periprosthetic joint infections (PJIs) require a special antimicrobial regimen, fundamentally different from an aseptic treatment, making a correct preoperative diagnosis essential. However, a successful preoperative microbe detection is not always possible. We wanted to find out (1) if a preoperative microbe detection is a prerequisite before starting a septic revision in suspected PJIs or if the preoperative diagnosis can solely be based on (para)clinical signs (persistent CRP >1 mg/dl, early X-ray loosening signs in the first 5 years, leucocytes joint aspiration >1700/µl, conspicuous history, and clinical signs like redness, pain, hyperthermia, swelling, and loss of function); (2) if patients with and without preoperative microbe detection have a different outcome; and (3) if the microbial growth is the most important criterion of a multifactorial PJI definition. Methods. We included all first-line two-stage hip (49) and knee (47) revisions, performed in our department from 06/2013 on, with an available 2-year follow-up. A PJI was defined as one of the following four criteria: fistula or purulence, Krenn Morawietz type 2 or 3, joint aspirate > 2000/μl leukocytes or >70% granulocytes, and microbial growth. This multifactorial PJI definition was based on the European Bone and Joint Infection Society (EBJIS). The standardized diagnostic algorithm is described in detail. Results. (1) 24 hip and 16 knee cases were treated without preoperative microbe detection solely on the basis of a (para)clinical diagnosis (see above). In the hip 91.6% (22 of 24 cases) showed an intraoperative microbe detection. In the knee, in 68.7% (11 of 16 cases) a microbe was detected intraoperatively and in 93.7% (15 of 16) at least one secure PJI criterion could be confirmed intraoperatively. (2) No statistical significant (p .517) difference between patients with (n = 56, reinfection rate 8.9%) and without (n = 40, 15%) preoperative microbe detection was found in a 2-year follow-up. (3) Microbial growth remains the overall (pre- and intraoperatively) most important criterion (hip 95.9%; knee 89.3%), followed by Krenn Morawietz for the intraoperative diagnosis (hip 67.3%, knee 48.9%), and joint aspiration for the knee and fistula for the hip, respectively, as preoperative criteria. Conclusion. High rates of intraoperatively fulfilled EBJIS PJI criteria show that a preoperative microbe detection is not necessary before intervening in suspected PJIs. The indication for a septic revision can solely be based on (para)clinical signs. The new established diagnostic algorithm based on a multifactorial PJI definition showed high precision in finding PJIs.

scholarly journals The obstacles facing Riyada- financed projects – A field study from the perspective of an entrepreneur in Asir region –: المعوقات التي تواجه المشاريع الممولة من ريادة – دراسة ميدانية من منظور ريادةً بمنطقة عسير –

2021 ◽  
Vol 5 (24) ◽  
pp. 158-142
Author(s):  
Dalia Mushabab Al- Qahtani Dalia Mushabab Al- Qahtani
Keyword(s):  
Field Study ◽  
Analytical Approach ◽  
High Ratio ◽  
Simple Random Sample ◽  
Average Ratio ◽  
Organizational Constraints ◽  
Job Opportunities ◽  
Research Population ◽  
Asir Region

The present research aimed to identify constraints facing projects funded by Riyadah: a field study from Riyadah's perspective in Assir region. And to study the hypotheses of the study represented in the presence of a statistically significant impact of the financing obstacles facing Riyada- financed projects in Aseer region The research was based on the descriptive and analytical approach, and the research population included all owners of the projects funded by Reyadah in Asir region, and a simple random sample consisted of (150) respondents was selected. The study concluded that the funding constraints facing projects funded by Riyadah in Assir region were of a average ratio, that the marketing constraints facing projects funded by Riyadah in Assir region were of high ratio, and that the administrative and organizational constraints facing projects funded by Riyadah in Assir region were of high ratio. Also, it was concluded that there is a statistically significant impact on each of (funding constraints, marketing constraints, and administrative & organizational constraints) facing projects funded by Riyadah in Assir region. The research recommended the necessity for Riyadah Institution to give the owners of the funded projects specialized courses in management and organization that help them in carrying out the tasks assigned to them, and the necessity to follow up the funded projects periodically so that the performance is evaluated on an ongoing basis and the mistakes are corrected. As well, Riyadah Institution has to provide help and assistance for various categories in order to create different job opportunities for many people.

scholarly journals Influence of culture conditions on extracellular polymeric substances production by the white rot fungi Phanerochaete chrysosporium

2018 ◽  
Vol 175 ◽  
pp. 01004
Author(s):  
Ningjie Li ◽  
Linbo Fu ◽  
Lei Wu ◽  
Zhongwei Chen ◽  
Qi Lan
Keyword(s):  
Heavy Metals ◽  
Phanerochaete Chrysosporium ◽  
Incubation Time ◽  
Extracellular Polymeric Substances ◽  
White Rot Fungi ◽  
Culture Conditions ◽  
White Rot ◽  
Influence Of Temperature ◽  
Model Strain

The extracellular polymeric substances of white rot fungi play an important role in the adsorption of heavy metals, but the influence of culture conditions on extracellular polymeric substances production is still unknown. In this paper, we researched on the influence of temperature, incubation time, the rotational speed and the inoculation volume on the yield of extracellular polymeric substances produced by Phanerochaete chrysosporium, a model strain of white rot fungi. The results show that the optimum culture conditions for Phanerochaete chrysosporium to produce extracellular polymeric substances was culturing at 40 °C, incubating for 5 d, rotating at 100 rpm, and inoculating 0.5 ml of spore suspension with concentration of 2.5×106 spores/ml. The highest yield of EPS was 234.65 mg/g when the fungi was cultured at 100 rpm, 40 °C and incubated for 5 days. This study can provide useful information for the follow-up experiments related to extracellular polymeric substances of white rot fungi

Decrease in Toxicity of Pentachlorophenol and Toluene to Growth of Selected Microbial Consortia and Activated Sludge by Pretreatment with Phanerochaete chrysosporium

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2125-2128 ◽  
Author(s):  
J. Bower Carberry ◽  
L. E. Kovach
Keyword(s):  
Activated Sludge ◽  
Phanerochaete Chrysosporium ◽  
Growth Rates ◽  
Specific Growth ◽  
White Rot ◽  
Microbial Consortia ◽  
Fungal Culture ◽  
Model Compounds ◽  
Fungal Pretreatment ◽  
Specific Growth Rates

The white rot fungus Phanerochaete chrysosporium can degrade toxic compounds under specific nutrient conditions. This attribute was utilized in order to determine the effect of fungal pretreatment on model compounds pentachlorophenol and toluene. The fungal culture was purchased from ATCC, cultured on dextrose agar, and the mycelia harvested to degrade the model compounds. P. chrysosporium was able to degrade up to 74% of initial pentachlorophenol concentration in eight days and up to 31% initial toluene in 31 hours. Specific growth rates of activated sludge and selected microbial consortia were determined on untreated and fungal-pretreated parent model compounds. Specific growth rates for activated sludge and selected microbial consortia were enhanced by fungal pretreatment of both model compounds. Specific growth rates indicated more efficient use of toluene by activated sludge than by toluene-selected microbial consortia, while pentachlorophenol- selected microbial consortia exhibited more favorable growth rates on pentachlorophenol than did activated sludge. Pretreatment results indicated that the toxicity of model compounds was reduced by fungal pretreatment. The growth rates were compared and used as an indication of toxicity reduction which can be exploited at contaminated waste sites or at industrial pretreatment facilities.

scholarly journals The Reality of Governance for the Financial and Administrative Control Authority in the Sultanate of Oman: Reality and Challenges

2021 ◽  
Vol 1 (4) ◽  
pp. 64-92
Author(s):  
Ahmad Abdollah Salim Al Mashaikhie ◽  
Mohd Nazri Mohd Noor
Keyword(s):  
Financial Institutions ◽  
Economic Performance ◽  
Analytical Approach ◽  
Sultanate Of Oman ◽  
Medium Term ◽  
Administrative Control ◽  
Control Authority ◽  
Control Body

This study aims to identify the reality of governance for the Financial and Administrative Control Authority in the Sultanate of Oman. The problem of the study is that the Financial and Administrative Control Authority in the Sultanate of Oman is making many efforts to popularize procedures and methods through the tasks carried out by the Financial and Administrative Control Authority to improve the economic performance of institutions and to discover financial and administrative violations that workers may commit during a certain period of time. The importance of governance is highlighted in many matters, most notably the need to respect the laws and regulations that institutions follow, and that governance contributes to exposing professional errors and increasing the experiences of workers, especially those working in the financial and administrative fields, in order to achieve the highest levels of job discipline. Governance contributes significantly to achieving the long- and medium-term goals of institutions and companies. The study followed the descriptive analytical approach to describe and analyze the role of governance in the financial and administrative control body in the Sultanate of Oman. The current study concluded that governance in the Financial and Administrative Control Authority in the Sultanate of Oman is based on several pillars, including work ethics in financial institutions and the continuity of oversight, follow-up and oversight. The study recommended the necessity of applying governance mechanisms in Omani institutions.

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