Biodiesel from mixed culture algae via a wet lipid extraction procedure

AbstractShotgun lipidomics enables an extensive analysis of lipids from tissues and fluids. Each specimen requires appropriate extraction and processing procedures to ensure good coverage and reproducible quantification of the lipidome. Adipose tissue (AT) has become a research focus with regard to its involvement in obesity-related pathologies. However, the quantification of the AT lipidome is particularly challenging due to the predominance of triacylglycerides, which elicit high ion suppression of the remaining lipid classes. We present a new and validated method for shotgun lipidomics of AT, which tailors the lipid extraction procedure to the target specimen and features high reproducibility with a linear dynamic range of at least 4 orders of magnitude for all lipid classes. Utilizing this method, we observed tissue-specific and diet-related differences in three AT types (brown, gonadal, inguinal subcutaneous) from lean and obese mice. Brown AT exhibited a distinct lipidomic profile with the greatest lipid class diversity and responded to high-fat diet by altering its lipid composition, which shifted towards that of white AT. Moreover, diet-induced obesity promoted an overall remodelling of the lipidome, where all three AT types featured a significant increase in longer and more unsaturated triacylglyceride and phospholipid species.The here presented method facilitates reproducible systematic lipidomic profiling of AT and could be integrated with further –omics approaches used in (pre-)clinical research, in order to advance the understanding of the molecular metabolic dynamics involved in the pathogenesis of obesity-associated disorders.

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Biodiesel and biogas production from Isochrysis galbana using dry and wet lipid extraction: A biorefinery approach

Renewable Energy ◽

10.1016/j.renene.2019.06.148 ◽

2020 ◽

Vol 146 ◽

pp. 188-195 ◽

Cited By ~ 9

Author(s):

Alejandra Sánchez-Bayo ◽

Daniel López-Chicharro ◽

Victoria Morales ◽

Juan José Espada ◽

Daniel Puyol ◽

...

Keyword(s):

Biogas Production ◽

Lipid Extraction ◽

Isochrysis Galbana ◽

Wet Lipid Extraction

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In situ solvent recovery by using hydrophobic/oleophilic filter during wet lipid extraction from microalgae

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-019-02141-6 ◽

2019 ◽

Vol 42 (9) ◽

pp. 1447-1455 ◽

Cited By ~ 2

Author(s):

Hogi Kim ◽

Jihye Shin ◽

Donghyo Lee ◽

Sung Gap Im ◽

Yong Keun Chang

Keyword(s):

Lipid Extraction ◽

Solvent Recovery ◽

Wet Lipid Extraction

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Novel strategy for microalgae cell disruption and wet lipid extraction by employing electro-Fenton process with sacrificial steel anode

Bioresource Technology ◽

10.1016/j.biortech.2021.126110 ◽

2021 ◽

pp. 126110

Author(s):

Wanniarachchige Paramitha Sandani ◽

Malith Premaratne ◽

Thilini U. Ariyadasa ◽

Jagath Kumara Premachandra

Keyword(s):

Lipid Extraction ◽

Cell Disruption ◽

Fenton Process ◽

Wet Lipid Extraction ◽

Novel Strategy

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Analysis of the main conjugated linoleic acid (CLA) precursors (C18:2 n-6 and C18:3 n-3) in Brachiaria ruzizienses by capillary zone electrophoresis

Open Chemistry ◽

10.2478/s11532-013-0262-z ◽

2013 ◽

Vol 11 (8) ◽

pp. 1286-1296 ◽

Cited By ~ 4

Author(s):

Renata Jesus Coelho Castro ◽

Fausto Sobrinho ◽

Marco Sundfeld da Gama ◽

Patrícia Castro Barra ◽

Rosemar Antoniassi ◽

...

Keyword(s):

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Capillary Zone Electrophoresis ◽

Lipid Extraction ◽

Extraction Procedure ◽

P Value ◽

Official Method ◽

Significant Difference ◽

Zone Electrophoresis ◽

Capillary Zone

AbstractAn alternative method for extraction optimization of C18:2 n-6 and C18:3 n-3, the main precursors for the synthesis of conjugated linoleic acid (CLA), in Brachiaria ruzizienses forages was proposed. Three methods of lipid extraction were tested: 1. Hara & Radin, 2. Micro Folch and 3. Bligh & Dyer. The preliminary test showed the Hara & Radin method as the most promising procedure. Then, a 33 Box Behnken design with triplicate in the central point was applied in Hara & Radin method in order to optimize the extraction procedure. The optimization extraction was monitored by quantification of C18:2 n-6 and C18:3 n-3 through capillary zone electrophoresis (CZE). The results obtained by CZE were compared to gas chromatography (AOCS official method) in real samples using the paired t-test. No significant difference between methods was found within a 95% confidence interval (p-value= 0.937). The alternative CZE method for Brachiaria ruzizienses forages analysis has some advantages in comparison with official GC method such as, short analysis time (10 min), no derivatization step for sample preparation, absence of specific separation columns, lower analytical cost and high throughput.

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Biomolecular Component Analysis of Phospholipids Composition in Live HeLa Cells

Biosensors ◽

10.3390/bios8040123 ◽

2018 ◽

Vol 8 (4) ◽

pp. 123 ◽

Cited By ~ 2

Author(s):

Svitlana Levchenko ◽

Junle Qu

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Hela Cells ◽

Unsaturated Fatty Acids ◽

Lipid Analysis ◽

Lipid Extraction ◽

Extraction Procedure ◽

Phospholipid Composition ◽

Component Analysis ◽

Saturation Degree

The alteration of the phospholipid composition within the cell, in particular the ratio between saturated and unsaturated fatty acids, can serve as an important biomarker to prognosis of the disease progression (e.g., fatty-liver disease, prostate cancer, or neurodegenerative disorders). Major techniques for lipid analysis in biological samples require a lipid extraction procedure that is not compatible with live cell studies. To address this challenge, we apply microRaman-Biomolecular Component Analysis (BCA) for comparative analysis of phospholipid composition and sensing the saturation degree of fatty acid lipid chain in live HeLa cells and lipids extracted from HeLa cells. After processing raw Raman data, acquired in lipid droplets (LDs) free cytoplasmic area, LDs and extracted lipids with BCA, the lipid component was isolated. Despite the similarity in general profiles of processed Raman spectra acquired in live cells and extracted lipids, some clear differences that reflect diversity in their phospholipids composition were revealed. Furthermore, using the direct relation between the number of double bonds in the fatty acid chain and the intensity ratio of the corresponding Raman bands, the saturation degree of fatty acids was estimated.

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Process evaluation data supporting studies on swing strategies to recover N-ethylbutylamine after wet lipid extraction from microalgae

Data in Brief ◽

10.1016/j.dib.2019.104416 ◽

2019 ◽

Vol 26 ◽

pp. 104416 ◽

Cited By ~ 1

Author(s):

Ying Du ◽

Veronika Cyprichová ◽

Kevin Hoppe ◽

Boelo Schuur ◽

Wim Brilman

Keyword(s):

Process Evaluation ◽

Lipid Extraction ◽

Evaluation Data ◽

Wet Lipid Extraction

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Bryocella elongata gen. nov., sp. nov., a member of subdivision 1 of the Acidobacteria isolated from a methanotrophic enrichment culture, and emended description of Edaphobacter aggregans Koch et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.031898-0 ◽

2012 ◽

Vol 62 (Pt_3) ◽

pp. 654-664 ◽

Cited By ~ 56

Author(s):

Svetlana N. Dedysh ◽

Irina S. Kulichevskaya ◽

Yulia M. Serkebaeva ◽

Maria A. Mityaeva ◽

Vladimir V. Sorokin ◽

...

Keyword(s):

Enrichment Culture ◽

Sequence Similarity ◽

Lipid Extraction ◽

Extraction Procedure ◽

Rrna Gene ◽

Emended Description ◽

Novel Genus And Species ◽

Mesophilic Bacterium ◽

Unknown Structure ◽

Membrane Spanning

An aerobic, pink-pigmented, chemo-organotrophic bacterium, designated strain SN10T, was isolated from a methanotrophic enrichment culture obtained from an acidic Sphagnum peat. This isolate was represented by Gram-negative, non-motile rods that multiply by normal cell division and form rosettes. Strain SN10T is an obligately acidophilic, mesophilic bacterium capable of growth at pH 3.2–6.6 (with an optimum at pH 4.7–5.2) and at 6–32 °C (with an optimum at 20–24 °C). The preferred growth substrates are sugars and several heteropolysaccharides of plant and microbial origin, such as pectin, lichenan, fucoidan and gellan gum. While not being capable of growth on C1 compounds, strain SN10T can develop in co-culture with exopolysaccharide-producing methanotrophs by utilization of their capsular material. The major fatty acids determined in strain SN10T using the conventional lipid extraction procedure are iso-C15 : 0 and C16 : 1ω7c. Upon hydrolysis of total cell material, substantial amounts of the uncommon membrane-spanning lipid 13,16-dimethyl octacosanedioic acid (isodiabolic acid) were also detected. The polar lipids are two phosphohexoses, phosphatidylethanolamine, phosphatidylglycerol and several phospholipids of unknown structure. The major quinone is MK-8. Pigments are carotenoids. The G+C content of the DNA is 60.7 mol%. Strain SN10T forms a separate lineage within subdivision 1 of the phylum Acidobacteria and displays 94.0–95.4 % 16S rRNA gene sequence similarity to members of the genera Edaphobacter and Granulicella, 93.0–93.7 % similarity to members of the genus Terriglobus and 92.2–92.3 % similarity to the type strains of Telmatobacter bradus and Acidobacterium capsulatum. Therefore, strain SN10T is classified within a novel genus and species, for which the name Bryocella elongata gen. nov., sp. nov. is proposed. Strain SN10T ( = LMG 25276T = DSM 22489T) is the type strain of Bryocella elongata. An emended description of Edaphobacter aggregans Koch et al. 2008 is also given.

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