Dual substrate strategy to enhance butanol production using high cell inoculum and its efficient recovery by pervaporation

2014 ◽  
Vol 152 ◽  
pp. 377-383 ◽  
Author(s):  
Sweta Yadav ◽  
Garima Rawat ◽  
Priyanka Tripathi ◽  
R.K. Saxena
Keyword(s):  
High Cell ◽  
Butanol Production ◽  
Efficient Recovery ◽  
Cell Inoculum ◽  
Dual Substrate

Enhancing butanol production with Clostridium pasteurianum CH4 using sequential glucose–glycerol addition and simultaneous dual-substrate cultivation strategies

2013 ◽  
Vol 135 ◽  
pp. 324-330 ◽  
Author(s):  
Wei-Chen Kao ◽  
De-Shun Lin ◽  
Chieh-Lun Cheng ◽  
Bor-Yann Chen ◽  
Chiu-Yue Lin ◽  
...  
Keyword(s):  
Clostridium Pasteurianum ◽  
Butanol Production ◽  
Dual Substrate

scholarly journals PHA Productivity and Yield ofRalstonia eutrophaWhen Intermittently or Continuously Fed a Mixture of Short Chain Fatty Acids

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Panchali Chakraborty ◽  
Kasiviswanathan Muthukumarappan ◽  
William R. Gibbons
Keyword(s):  
Fermentation Process ◽  
Cost Effective ◽  
Short Chain Fatty Acids ◽  
Feeding Strategies ◽  
Corn Ethanol ◽  
High Cell ◽  
Pha Production ◽  
Condensed Corn Solubles ◽  
Cell Densities ◽  
Dual Substrate

The research described in this present study was part of a larger effort focused on developing a dual substrate, dual fermentation process to produce Polyhydroxyalkanoate (PHA). The focus of this study was developing and optimizing a strategy for feeding a mixture of SCFAs (simulated ARF) and maximizing PHA production in a cost-effective way. Three different feeding strategies were examined in this study. The substrate evaluated in this study for the growth phase ofR. eutrophawas condensed corn solubles, a low-value byproduct of the dry-mill, corn ethanol industry. The culture was grown to high cell densities in nitrogen-supplemented condensed corn solubles media in 5 L bioreactors. The overall growth rate ofR. eutrophawas 0.2 h−1. The 20 mL ARF feeding every 3 h from 48 to 109 h strategy gave the best results in terms of PHA production. PHA productivity (0.0697 g L−1 h−1), PHA concentration (8.37 g L−1), and PHA content (39.52%) were the highest when ARF was fed every 3 h for 61 h. This study proved that condensed corn solubles can be potentially used as a growth medium to boost PHA production byR. eutrophathus reducing the overall cost of biopolymer production.

scholarly journals Biobutanol Production Using High Cell Density Fermentation in a Large Extractant Volume

2020 ◽  
Vol 9 (3) ◽  
Author(s):  
Rizki Fitria Darmayanti ◽  
Yukihiro Tashiro ◽  
Kenji Sakai ◽  
Kenji Sanomoto ◽  
Ari Susanti ◽  
...  
Keyword(s):  
Cell Density ◽  
Oleyl Alcohol ◽  
High Cell Density ◽  
High Cell ◽  
Butanol Production ◽  
Producer Strain ◽  
Toxicity Effect ◽  
Minimum Medium ◽  
Butanol Concentration

Biobutanol is well-known as a suitable substitute for gasoline which can be applied without enginemodification. Butanol toxicity to the producer strain causes difficulties to grow strain with more than 4 g/L dry cellweight and to produce butanol more than 20 g/L. Fermentation with high initial cell density was reported to enhancebutanol productivity. In addition, oleyl alcohol has been recognized to perform effective extraction for butanol because ofits selectivity and biocompatibility so that reducing toxicity effect. Butanol fermentation with high cell density and largeextractant volume has not been reported and is expected to improve butanol production in minimum medium volume.Clostridium saccharoperbutylacetonicum N1-4, C. beijerinckii NCIMB 8052 (8052), and C. acetobutylicum ATCC 824(824) were used in this study. Three kinds of media, TYA, TY, and TY-CaCO3, were used to investigate in conventionalextractive fermentation. Then, in situ extractive fermentations with Ve/Vb ratios at 0.1, 0.5, 1.0, and 10 were operated.Total butanol concentration was defined as the broth based total butanol, that is total amounts of butanol produced inbroth and extractant per the volume of broth. TYA medium resulted the highest total butanol concentrations by N1-4 (12g/L), 8052 (11 g/L), and 824 (15 g/L) and the highest partition coefficient (3.7) among the three media with Ve/Vb ratiosat 0.5. N1-4 yielded the highest increment of total butanol production (22 g/L) in the extractive fermentation with highcell density. Low butanol concentration of 0.8 g/L butanol in broth was maintained with the extractant to broth volumeratio (Ve/Vb), which was much lower than 4.4 g/L with the ratio of 0.5. Ve/Vb ratio of 10 provided 2-fold higher totalbutanol concentration (28 g/L) than that 11 g/L obtained with Ve/Vb ratio of 0.5. These results indicated that largervolume of extractant to broth improved total butanol concentration by reducing butanol toxicity and led to high mediumbasedbutanol yield in fermentation using high cell density.

EVALUATION OF FLOAT TRAYS WITH HIGH CELL NUMBERS ON STAND COUNTS AND YIELDS IN A CLOSE-GROWN TOBACCO PRODUCTION SYSTEM

2012 ◽  
Vol 49 ◽  
pp. 4-7 ◽  
Author(s):  
Richard Mundell ◽  
Orlando Chambers ◽  
James P O'Daniel ◽  
H. Maelor Davies
Keyword(s):  
Production System ◽  
High Cell ◽  
Cell Numbers ◽  
Tobacco Production

BIO-FUEL PRODUCTION FROM CARBONDIOXIDE GAS USING S. elongatus PCC 7942 FROM CYANOBACTERIA

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Delia Teresa Sponza ◽  
Cansu Doğanx
Keyword(s):  
Flow Rate ◽  
Maximum Yield ◽  
Butanol Production ◽  
Fuel Production ◽  
Cost Analyses ◽  
Operational Conditions ◽  
O2 Concentration ◽  
The Cost ◽  
Pcc 7942 ◽  
Dissolved O2

The scope of this study, is  1-butanol production from CO2 with S. elongatus PCC 7942 culture. The yields of 1-butanolproduced/CO2utilized have been calculated. The maximum concentration of produced 1- butanol is 35.37 mg/L and 1-butanolproduced/CO2utilized efficiency is 92.4. The optimum operational conditions were  30°C temperature, 60 W intensity of light, pH= 7.1, 120 mV redox potential, 0.083 m3/sn flow rate with CO2 and 0.5 mg/l dissolved O2 concentration. Among the enzymes on the metabolic trail of the production of 1-butanol via using S. elongatus PCC 7942 cyanobacteria. At maximum yield; the measured concentrations are 0.016 µg/ml for hbd; 0.0022 µg/ml for Ter and 0.0048 µg/ml for AdhE2. The cost analyses necessary for 1-butanol production has been done and the cost of 1 litre 1-butanol has been determined as maximum 1.31 TL/L.

A quantitative evaluation of some factors affecting the non-fatty solids of cow's milk

1960 ◽  
Vol 27 (1) ◽  
pp. 19-32 ◽  
Author(s):  
W. H. Alexander ◽  
F. B. Leech
Keyword(s):  
Cell Count ◽  
Residual Effect ◽  
Clinical Mastitis ◽  
High Cell ◽  
Factors Affecting ◽  
Stage Of Lactation ◽  
Cell Counts ◽  
Satisfactory Analysis ◽  
The Difference ◽  
Image Position

SummaryTen farms in the county of Durham took part in a field study of the effects of feeding and of udder disease on the level of non-fatty solids (s.n.f.) in milk. Statistical analysis of the resulting data showed that age, pregnancy, season of the year, and total cell count affected the percentage of s.n.f. and that these effects were additive and independent of each other. No effect associated with nutritional changes could be demonstrated.The principal effects of the factors, each one freed from effects of other factors, were as follows:Herds in which s.n.f. had been consistently low over a period of years were compared with herds in which s.n.f. had been satisfactory. Analysis of the data showed that about 70% of the difference in s.n.f. between these groups could be accounted for by differences in age of cow, stage of lactation, cell count and breed.There was some evidence of a residual effect following clinical mastitis that could not be accounted for by residual high cell counts.The within-cow regression of s.n.f. on log cell count calculated from the Durham data and from van Rensburg's data was on both occasions negative.The implications of these findings are discussed, particularly in relation to advisory work.

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