Efficient saccharification of agave biomass using Aspergillus niger produced low-cost enzyme cocktail with hyperactive pectinase activity

2019 ◽  
Vol 272 ◽  
pp. 26-33 ◽  
Author(s):  
Jiahong Wang ◽  
Chonlong Chio ◽  
Xuntong Chen ◽  
Erzheng Su ◽  
Fuliang Cao ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Low Cost ◽  
Enzyme Cocktail ◽  
Pectinase Activity

scholarly journals Cocktail of carbohydrases from Aspergillus niger: an economical and eco-friendly option for biofilm clearance from biopolymer surfaces

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Arashdeep Kaur ◽  
Sanjeev Kumar Soni ◽  
Shania Vij ◽  
Praveen Rishi
Keyword(s):  
Aspergillus Niger ◽  
Laser Scanning ◽  
Statistical Modelling ◽  
Laser Scanning Microscopy ◽  
Enzyme Treatment ◽  
Confocal Laser ◽  
Abiotic Surfaces ◽  
Enzyme Cocktail ◽  
Natural Variant

AbstractBiofilm formation on both biotic and abiotic surfaces accounts for a major factor in spread of antimicrobial resistance. Due to their ubiquitous nature, biofilms are of great concern for environment as well as human health. In the present study, an integrated process for the co-production of a cocktail of carbohydrases from a natural variant of Aspergillus niger was designed. The enzyme cocktail was found to have a noteworthy potential to eradicate/disperse the biofilms of selected pathogens. For application of enzymes as an antibiofilm agent, the enzyme productivities were enhanced by statistical modelling using response surface methodology (RSM). The antibiofilm potential of the enzyme cocktail was studied in terms of (i) in vitro cell dispersal assay (ii) release of reducing sugars from the biofilm polysaccharides (iii) the effect of enzyme treatment on biofilm cells and architecture by confocal laser scanning microscopy (CLSM). Potential of the enzyme cocktail to disrupt/disperse the biofilm of selected pathogens from biopolymer surfaces was also assessed by field emission scanning electron microscopy (FESEM) analysis. Further, their usage in conjunction with antibiotics was assessed and it was inferred from the results that the use of enzyme cocktail augmented the efficacy of the antibiotics. The study thus provides promising insights into the prospect of using multiple carbohydrases for management of heterogeneous biofilms formed in natural and clinical settings.

scholarly journals Pectinase production by Aspergillus niger isolated from decomposed apple skin

2013 ◽  
Vol 48 (1) ◽  
pp. 25-32 ◽  
Author(s):  
S Islam ◽  
B Feroza ◽  
AKMR Alam ◽  
S Begum
Keyword(s):  
Aspergillus Niger ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Inoculum Size ◽  
Point Of View ◽  
Media Composition ◽  
Maximal Level ◽  
Pectinolytic Activity ◽  
Pectinase Activity ◽  
Pectinase Production

Pectinase activity among twelve different fungal strains, Aspergillus niger IM09 was identified as a potential one to produce maximal level 831 U/g at pH 4.0. Media composition, incubation temperature, incubation time, substrate concentration, aeration, inoculum size, assay temperature and nitrogen sources were found to effect pectinase activity. Moisture content did not affect the activity significantly. Media composition was varied to optimize the enzyme production in solid state fermentation. It was observed that the highest pectinase activity of 831.0 U/g was found to produce in presence of yeast extract as a nitrogen source in combination with ammonium sulfate in assay media. Aeration showed positive significant effects on pectinase production 755 U/g at 1000 ml flasks. The highest pectinase production was found at 2 g pectin (521 U/g) used as a substrate. Pectinolytic activity was found to have undergone catabolite repression with higher pectin concentration (205 U/g at 5 g pectin). The incubation period to achieve maximum pectinase activity by the isolated strain Aspergillus niger IM09 was 3 days, which is suitable from the commercial point of view. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15410 Bangladesh J. Sci. Ind. Res. 48(1), 25-32, 2013

Construction of the Starter to Produce Lignocellulases via Solid State Fermentation of Rice Straw

2011 ◽  
Vol 356-360 ◽  
pp. 1819-1822
Author(s):  
Shan Shan Du ◽  
Lu Yang ◽  
Jian Zhang Lu ◽  
Qing Qing Li ◽  
Juan Xu ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Rice Straw ◽  
Solid State Fermentation ◽  
Low Cost ◽  
Natural Microflora ◽  
Special Strain

The study demonstrates the starter construction and its solid state fermentation (SSF) using rice straw as substrate for lignocellulases production. The starter is constructed by natural microflora growing on rice straw additionally reinforced with Aspergillus niger ZJU-RYD1, using the SSF substrate rice straw with 90% moisture content, successfully produced the integrate and high active lignocellulases at about 72 h, i.e. CMCase 12.44 U/g, cellobiase 12.05 U/g, FPAase 3.18 U/g, xylanase 365.61 U/g, lassase 2.48 U/g. In this study the strategy is satisfactorily developed to construct the starter for high effective and low-cost production of lignocellulases using rice straw as substrate, based on natural microflora and additional reinforcing with special strain.

scholarly journals Bioremoval of Different Heavy Metals in Industrial Effluent by the Resistant Fungal Strain Aspergillus niger

2021 ◽  
Vol 20 (4) ◽  
Author(s):  
K. J. Naveen Kumar ◽  
J. Prakash
Keyword(s):  
Heavy Metals ◽  
Aspergillus Niger ◽  
Metal Binding ◽  
Metal Removal ◽  
Low Cost ◽  
Heavy Metal Removal ◽  
Industrial Effluent ◽  
Toxic Metal ◽  
Differential Pulse ◽  
Toxic Heavy Metals

Developing countries are increasingly concerned with pollution due to toxic heavy metals in the environment. Unlike most organic pollutants which can be destroyed, toxic metal ions released into the environment often persist indefinitely circulating and eventually accumulating throughout the food chain thus posing a serious threat to mankind. The use of biological materials for heavy metal removal or recovery has gained importance in recent years due to their good performance and low cost. Among the various sources, both live and inactivated biomass of organisms exhibits interesting metal binding capacities. Their complex cell walls contain high content of functional groups like amino, amide, hydroxyl, carboxyl, and phosphate which have been implicated in metals binding. In the present study, Aspergillus niger was used to analyze the metal uptake from an aqueous solution. The determination of Cu+2, Pb+2, Cd+2, Zn+2, Co-2 and Ni+2 in samples was carried out by differential Pulse Anodic Voltammetry (DPASV) and the Voltammograms. Production of oxalic acid was carried out by submerged fermentation. The organism used in the present study has the ideal properties to sequester toxic metals and grow faster.

scholarly journals Evaluation of agricultural byproducts for the production of betaglucosidase by Aspergillus niger MBT-2 using solid state fermentation

2020 ◽  
Vol 49 (1) ◽  
pp. 135-140
Author(s):  
Roheena Abdullah ◽  
Maria Hanif ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Kinza Nisar ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Low Cost ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Enhanced Production ◽  
Enzyme Productivity ◽  
Valuable Product ◽  
By Products

The experiment was conducted to isolate and screen fungal strain and optimization of solid-state fermentation conditions for enhanced production of β-glucosidase. Different fungal cultures were isolated and screened for β-glucosidase production. The physicochemical and nutritional parameters were optimized for enhanced production of β-glucosidase from higher producer. Among all the isolates the isolate which exhibited highest β-glucosidase potential was identified and assigned the code as Aspergillus niger MBT-2. The optimum β-glucosidase production was obtained in M5 medium containing wheat bran after 72 hrs of incubation at 40°C, pH 6 and 20 ml of moisture contents. In addition to this 2% fructose and 2% yeast extract proved to be best carbon and nitrogen sources, respectively and gave maximal enzyme productivity. The exploitation of agricultural by products as a substrate reduced the production cost of enzyme and makes the process economical. The Aspergillus niger MBT-2 has promising potential of bioconversion of low-cost material into valuable product like β-glucosidase.

scholarly journals A novel strain of Aspergillus niger producing a cocktail of hydrolytic depolymerising enzymes for the production of second generation biofuels

BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 552-569 ◽  
Author(s):  
Namita Bansal ◽  
Rupinder Tewari ◽  
Jugal Kishore Gupta ◽  
Raman Soni ◽  
Sanjeev Kumar Soni
Keyword(s):  
Aspergillus Niger ◽  
Second Generation ◽  
Hydrolytic Enzymes ◽  
Reducing Sugar ◽  
Soil Samples ◽  
Domestic Waste ◽  
Second Generation Biofuels ◽  
Pectinolytic Enzymes ◽  
Enzyme Cocktail ◽  
Conversion Efficiencies

The screening and isolation of fungi producing a cocktail of hydrolytic enzymes was studied. Among the various isolates obtained from different soil samples, a strain NS-2 was selected. The phylogenetic analysis of this strain showed highest homology (99%) with Aspergillus niger. It was capable of producing cellulolytic, hemicellulolytic, amylolytic, and pectinolytic enzymes in appreciable titers on wheat bran based liquid and solid state media. The mixture of enzymes produced by this organism could effectively hydrolyze various domestic waste residues, revealing conversion efficiencies of 89 to 92% and produced high reducing sugar yields of 0.48 to 0.66 g/g of dry residue. This enzyme cocktail could potentially find a significant application in the conversion of agricultural and other waste residues having cellulose, hemicellulose, starch, and pectin as carbohydrates to produce simpler sugars which can be fermented for the production of second generation biofuels.

scholarly journals Evaluation of Filamentous Fungi and Inducers for the Production of Endo-Polygalacturonase by Solid State Fermentation

2002 ◽  
Vol 57 (7-8) ◽  
pp. 666-670 ◽  
Author(s):  
Adriana B. Dartora ◽  
Telma E. Bertolin ◽  
Denise Bilibio ◽  
Mauricio M. Silveira ◽  
Jorge A. V. Costa
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Filamentous Fungi ◽  
Solid State Fermentation ◽  
Low Cost ◽  
Aspergillus Awamori ◽  
Trichoderma Sp ◽  
Sweet Lime ◽  
Pectinolytic Activity ◽  
Opening Up

Five strains of filamentous fungi (Aspergillus niger strains NRRL 3122 and T0005007-2, Aspergillus oryzae CCT 3940, Aspergillus awamori NRRL 3112 and a Trichoderma sp.) were compared for their capacity to produce endo-polygalacturonase (endo-PG) in solid state fermentation. Maximum pectinolytic activity was reached in 72 h of growth, the best two fungal strains being A. niger T0005007-2 and A. oryzae CCT 3940. Three types of commercial purified pectin and four of unprocessed pectin (tangerine, orange, Tahiti lime and sweet lime rind) were used to assess the effect of pectin on the production of endo-PG by A. niger T0005007-2. Maximum pectinolytic activity was achieved using 6 and 10% (w/w) of purified pectin as inducer. Depending on the origin of the commercial pectin used as inducer, maximum endo-PG levels varied from 223 to 876 units per gram of dry medium (one endo-PG unit (U) was defined as the quantity of enzyme which caused a reduction in viscosity of 50% in a 1% w/v solution of pectin in 30 min), indicating that care should be taken when choosing this component of the medium. When the crude pectins were used as inducers at the same concentration as purified pectin, maximum endo-PG activities were 250-300 units/g. However, by increasing the amount of Tahiti lime rind to 50% (w/w) maximum endo-PG was 919 U/g, thus opening up the possibility of a low cost medium for endo-PG production.

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