scholarly journals Beyond PHA: Stimulating intracellular accumulation of added-value compounds in mixed microbial cultures

2021 ◽  
pp. 125381
Author(s):  
F. Pinto-Ibieta ◽  
A. Serrano ◽  
M. Cea ◽  
G. Ciudad ◽  
F.G. Fermoso
Keyword(s):  
Added Value ◽  
Intracellular Accumulation ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures

scholarly journals From Food Waste to Volatile Fatty Acids towards a Circular Economy

2021 ◽  
Author(s):  
Mónica Carvalheira ◽  
Anouk F. Duque
Keyword(s):  
Fatty Acids ◽  
Food Waste ◽  
Circular Economy ◽  
Volatile Fatty Acids ◽  
Animal Feed ◽  
Anaerobic Fermentation ◽  
Industrial Sector ◽  
Added Value ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures

The food industrial sector generates large amounts of waste, which are often used for animal feed, for agriculture or landfilled. However, these wastes have a very reach composition in carbon and other compounds, which make them very attractive for valorization through biotechnological processes. Added value compounds, such as volatile fatty acids (VFAs), can be produced by anaerobic fermentation using pure cultures or mixed microbial cultures and food waste as carbon source. Research on valuable applications for VFAs, such as polyhydroxyalkanoates, bioenergy or biological nutrient removal, towards a circular economy is emerging. This enhances the sustainability and the economic value of food waste. This chapter reviews the various types of food waste used for VFAs production using mixed microbial cultures, the anaerobic processes, involved and the main applications for the produced VFAs. The main parameters affecting VFAs production are also discussed.

scholarly journals Assessment of Long-Term Fermentability of PHA-Based Materials from Pure and Mixed Microbial Cultures for Potential Environmental Applications

Water ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 897
Author(s):  
Neda Amanat ◽  
Bruna Matturro ◽  
Marta Maria Rossi ◽  
Francesco Valentino ◽  
Marianna Villano ◽  
...  
Keyword(s):  
Environmental Remediation ◽  
Volatile Fatty Acids ◽  
Rapid Onset ◽  
Pilot Scale ◽  
Point Of View ◽  
Microbial Cultures ◽  
Extraction And Purification ◽  
Fermentation Pattern ◽  
Mixed Microbial Cultures

The use of polyhydroxyalkanoates (PHA) as slow-release electron donors for environmental remediation represents a novel and appealing application that is attracting considerable attention in the scientific community. In this context, here, the fermentation pattern of different types of PHA-based materials has been investigated in batch and continuous-flow experiments. Along with commercially available materials, produced from axenic microbial cultures, PHA produced at pilot scale by mixed microbial cultures (MMC) using waste feedstock have been also tested. As a main finding, a rapid onset of volatile fatty acids (VFA) production was observed with a low-purity MMC-deriving material, consisting of microbial cells containing 56% (on weight basis) of intracellular PHA. Indeed, with this material a sustained, long-term production of organic acids (i.e., acetic, propionic, and butyric acids) was observed. In addition, the obtained yield of conversion into acids (up to 70% gVFA/gPHA) was higher than that obtained with the other tested materials, made of extracted and purified PHA. These results clearly suggest the possibility to directly use the PHA-rich cells deriving from the MMC production process, with no need of extraction and purification procedures, as a sustainable and effective carbon source bringing remarkable advantages from an economic and environmental point of view.

scholarly journals Use of Bioproducts Derived from Mixed Microbial Cultures Grown with Crude Glycerol to Protect Recycled Concrete Surfaces

Materials ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 2057
Author(s):  
Lorena Serrano-González ◽  
Daniel Merino-Maldonado ◽  
Manuel Ignacio Guerra-Romero ◽  
Julia María Morán-del Pozo ◽  
Paulo Costa Lemos ◽  
...  
Keyword(s):  
Water Absorption ◽  
Crude Glycerol ◽  
Protective Layer ◽  
Recycled Concrete ◽  
Recycled Aggregate ◽  
World Population ◽  
Management Problem ◽  
Waste Glycerol ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures

The large increase in the world population has resulted in a very large amount of construction waste, as well as a large amount of waste glycerol from transesterification reactions of acyl glycerides from oils and fats, in particular from the production of biodiesel. Only a limited percentage of these two residues are recycled, which generates a large management problem worldwide. For that reason, in this study, we used crude glycerol as a carbon source to cultivate polyhydroxyalkanoates (PHA)-producing mixed microbial cultures (MMC). Two bioproducts derived from these cultures were applied on the surface of concrete with recycled aggregate to create a protective layer. To evaluate the effect of the treatments, tests of water absorption by capillarity and under low pressure with Karsten tubes were performed. Furthermore, SEM-EDS analysis showed the physical barrier caused by biotreatments that produced a reduction on capillarity water absorption of up to 20% and improved the impermeability of recycled concrete against the penetration of water under pressure up to 2.7 times relative to the reference. Therefore, this bioproduct shown to be a promising treatment to protect against penetration of water to concrete surfaces increasing its durability and useful life.

Is the naphthalene sulfonate H-acid biodegradable in mixed microbial cultures under aerobic conditions?

2011 ◽  
Vol 102 (10) ◽  
pp. 5589-5595 ◽  
Author(s):  
T. Olmez-Hanci ◽  
I. Arslan-Alaton ◽  
D. Orhon ◽  
O. Karahan ◽  
E. Ubay Cokgor ◽  
...  
Keyword(s):  
Aerobic Conditions ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures

scholarly journals Electro-fermentation and redox mediators enhance glucose conversion into butyric acid with mixed microbial cultures

2019 ◽  
Vol 130 ◽  
pp. 107333 ◽  
Author(s):  
Paola Paiano ◽  
Miriam Menini ◽  
Marco Zeppilli ◽  
Mauro Majone ◽  
Marianna Villano
Keyword(s):  
Butyric Acid ◽  
Redox Mediators ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures

scholarly journals Volatile Compounds Originating from Mixed Microbial Cultures on Building Materials under Various Humidity Conditions

1998 ◽  
Vol 64 (8) ◽  
pp. 2914-2919 ◽  
Author(s):  
Anne Korpi ◽  
Anna-Liisa Pasanen ◽  
Pertti Pasanen
Keyword(s):  
Volatile Compounds ◽  
Microbial Activity ◽  
High Performance ◽  
Building Materials ◽  
Fungal Growth ◽  
Fungal Species ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures ◽  
Drying Conditions ◽  
Dilution Plate Method

ABSTRACT We examined growth of mixed microbial cultures (13 fungal species and one actinomycete species) and production of volatile compounds (VOCs) in typical building materials in outside walls, separating walls, and bathroom floors at various relative humidities (RHs) of air. Air samples from incubation chambers were adsorbed on Tenax TA and dinitrophenylhydrazine cartridges and were analyzed by thermal desorption-gas chromatography and high-performance liquid chromatography, respectively. Metabolic activity was measured by determining CO2 production, and microbial concentrations were determined by a dilution plate method. At 80 to 82% RH, CO2 production did not indicate that microbial activity occurred, and only 10% of the spores germinated, while slight increases in the concentrations of some VOCs were detected. All of the parameters showed that microbial activity occurred at 90 to 99% RH. The microbiological analyses revealed weak microbial growth even under drying conditions (32 to 33% RH). The main VOCs produced on the building materials studied were 3-methyl-1-butanol, 1-pentanol, 1-hexanol, and 1-octen-3-ol. In some cases fungal growth decreased aldehyde emissions. We found that various VOCs accompany microbial activity but that no single VOC is a reliable indicator of biocontamination in building materials.

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