Insights into enhanced polyhydroxyalkanoate production by the synergistic use of waste wood hydrolysate and volatile fatty acids by mixed microbial cultures

2021 ◽  
pp. 125488
Author(s):  
Dongna Li ◽  
Xiaojun Ma ◽  
Jianing Li ◽  
Binqing Sun
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Waste Wood ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures ◽  
Wood Hydrolysate

scholarly journals From Food Waste to Volatile Fatty Acids towards a Circular Economy

2021 ◽  
Author(s):  
Mónica Carvalheira ◽  
Anouk F. Duque
Keyword(s):  
Fatty Acids ◽  
Food Waste ◽  
Circular Economy ◽  
Volatile Fatty Acids ◽  
Animal Feed ◽  
Anaerobic Fermentation ◽  
Industrial Sector ◽  
Added Value ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures

The food industrial sector generates large amounts of waste, which are often used for animal feed, for agriculture or landfilled. However, these wastes have a very reach composition in carbon and other compounds, which make them very attractive for valorization through biotechnological processes. Added value compounds, such as volatile fatty acids (VFAs), can be produced by anaerobic fermentation using pure cultures or mixed microbial cultures and food waste as carbon source. Research on valuable applications for VFAs, such as polyhydroxyalkanoates, bioenergy or biological nutrient removal, towards a circular economy is emerging. This enhances the sustainability and the economic value of food waste. This chapter reviews the various types of food waste used for VFAs production using mixed microbial cultures, the anaerobic processes, involved and the main applications for the produced VFAs. The main parameters affecting VFAs production are also discussed.

scholarly journals Influence of the total concentration and the profile of volatile fatty acids on polyhydroxyalkanoates (PHA) production by mixed microbial cultures

2021 ◽  
Author(s):  
Gloria Bravo-Porras ◽  
Luis A. Fernández-Güelfo ◽  
Carlos J. Álvarez-Gallego ◽  
María Carbú ◽  
Diego Sales ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Total Concentration ◽  
Degradation Process ◽  
Microbial Cultures ◽  
Dry Cell Weight ◽  
Cheap Alternative ◽  
Mixed Microbial Cultures ◽  
Pha Production ◽  
Dry Cell

AbstractPolyhydroxyalkanoates (PHAs) production from lignocellulosic biomass using mixed microbial cultures (MMC) is a potential cheap alternative for reducing the use of petroleum-based plastics. In this study, an MMC adapted to acidogenic effluent from dark fermentation (DF) of exhausted sugar beet cossettes (ESBC) has been tested in order to determine its capability to produce PHAs from nine different synthetic mixtures of volatile fatty acids (VFAs). The tests consisted of mixtures of acetic, propionic, butyric, and valeric acids in the range of 1.5–9.0 g/L of total acidity and with three different valeric:butyric ratios (10:1, 1:1, and 1:10). Experimental results have shown a consistent preference of the MMC for the butyric and valeric acids as carbon source instead other shorter acids (propionic or acetic) in terms of PHA production yield (estimated in dry cell weight basis), with a maximum value of 23% w/w. Additionally, valeric-rich mixtures have demonstrated to carry out a fast degradation process but with poor final PHA production compared with high butyric mixtures. Finally, high initial butyric and valeric concentrations (1.1 g/L and 4.1 g/L) have demonstrated to be counterproductive to PHA production.

scholarly journals Bio-based conversion of volatile fatty acids from waste streams to polyhydroxyalkanoates using mixed microbial cultures

2021 ◽  
Vol 323 ◽  
pp. 124604
Author(s):  
Mariel Perez-Zabaleta ◽  
Merve Atasoy ◽  
Kasra Khatami ◽  
Elsa Eriksson ◽  
Zeynep Cetecioglu
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Waste Streams ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures

scholarly journals Assessment of Long-Term Fermentability of PHA-Based Materials from Pure and Mixed Microbial Cultures for Potential Environmental Applications

Water ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 897
Author(s):  
Neda Amanat ◽  
Bruna Matturro ◽  
Marta Maria Rossi ◽  
Francesco Valentino ◽  
Marianna Villano ◽  
...  
Keyword(s):  
Environmental Remediation ◽  
Volatile Fatty Acids ◽  
Rapid Onset ◽  
Pilot Scale ◽  
Point Of View ◽  
Microbial Cultures ◽  
Extraction And Purification ◽  
Fermentation Pattern ◽  
Mixed Microbial Cultures

The use of polyhydroxyalkanoates (PHA) as slow-release electron donors for environmental remediation represents a novel and appealing application that is attracting considerable attention in the scientific community. In this context, here, the fermentation pattern of different types of PHA-based materials has been investigated in batch and continuous-flow experiments. Along with commercially available materials, produced from axenic microbial cultures, PHA produced at pilot scale by mixed microbial cultures (MMC) using waste feedstock have been also tested. As a main finding, a rapid onset of volatile fatty acids (VFA) production was observed with a low-purity MMC-deriving material, consisting of microbial cells containing 56% (on weight basis) of intracellular PHA. Indeed, with this material a sustained, long-term production of organic acids (i.e., acetic, propionic, and butyric acids) was observed. In addition, the obtained yield of conversion into acids (up to 70% gVFA/gPHA) was higher than that obtained with the other tested materials, made of extracted and purified PHA. These results clearly suggest the possibility to directly use the PHA-rich cells deriving from the MMC production process, with no need of extraction and purification procedures, as a sustainable and effective carbon source bringing remarkable advantages from an economic and environmental point of view.

scholarly journals Effect of the solid retention time in the obtention of polyhydroxyalkanoates

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 864
Author(s):  
Rolando Calero ◽  
Manuel Martínez
Keyword(s):  
Fatty Acids ◽  
Retention Time ◽  
Volatile Fatty Acids ◽  
Batch Reactor ◽  
Oxygen Demand ◽  
Solid Retention Time ◽  
Dry Cell Weight ◽  
Acidogenic Reactor ◽  
Mixed Microbial Cultures ◽  
Dry Cell

Background: The effect of solid retention time (SRT) over cheese whey substrates in a fermentation process drives changes in the composition of polyhydroxyalkanoates (PHAs) obtained. Volatile fatty acids produced in the first step of an anaerobic sequencing batch reactor were used as substrates to produce PHA using mixed microbial cultures under aerobic dynamic feeding conditions. Methods: Analytical methods were used for the standard analysis of parameters of interest including measuring the amount of ammonium and phosphate, chemical oxygen demand, among others. Results: The SRT increasing from 4 to 6 and 10 days produced changes in the distribution of volatile fatty acids produced. The polyhydroxybutyrate-hydroxyvalerate copolymers formed in the accumulation stage gave the following results: 58:42, 68:32 and 81:19 (%), referred to SRTs of 10, 6 and 4 days, respectively. The maximum PHA accumulation obtained at 10 days of SRT was 52% of the dry cell weight within 7 h, reaching a PHA productivity of 0.62 g L−1 h−1 and a storage yield of 0.37. Conclusion: The SRT variation impact on the distribution of volatile fatty acids in the acidogenic reactor and consequently on the PHA production and composition formed in the accumulation stage.

scholarly journals The effect of methane and odd-chain fatty acids on 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) synthesis by a Methylosinus-dominated mixed culture

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Pawarisa Luangthongkam ◽  
Peter James Strong ◽  
Syarifah Nuraqmar Syed Mahamud ◽  
Paul Evans ◽  
Paul Jensen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Reducing Power ◽  
Valeric Acid ◽  
Continuous Reactor ◽  
Carbon And Nitrogen ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures ◽  
Odd Chain Fatty Acids ◽  
Aseptic Conditions ◽  
Chain Fatty Acids

AbstractA methanotrophic community was enriched in a semi-continuous reactor under non-aseptic conditions with methane and ammonia as carbon and nitrogen source. After a year of operation, Methylosinus sp., accounted for 80% relative abundance of the total sequences identified from potential polyhydroxyalkanoates (PHAs) producers, dominated the methane-fed enrichment. Prior to induction of PHA accumulation, cells harvested from the parent reactor contained low level of PHA at 4.0 ± 0.3 wt%. The cells were later incubated in the absence of ammonia with various combinations of methane, propionic acid, and valeric acid to induce biosynthesis of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). Previous studies reported that methanotrophic utilization of odd-chain fatty acids for the production of PHAs requires reducing power from methane oxidation. However, our findings demonstrated that the PHB-containing methanotrophic enrichment does not require methane availability to generate 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV)—when odd-chain fatty acids are presented. The enrichment yielded up to 14 wt% PHA with various mole fractions of 3HV monomer depending on the availability of methane and odd-fatty acids. Overall, the addition of valeric acid resulted in a higher PHA content and a higher 3HV fraction. The highest 3HV fraction (up to 65 mol%) was obtained from the methane–valeric acid experiment, which is higher than those previously reported for PHA-producing methanotrophic mixed microbial cultures.

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