Glial-fibrillary-acidic-protein (GFAP) biomarker detection in serum-matrix: Functionalization strategies and detection by an ultra-high-frequency surface-acoustic-wave (UHF-SAW) lab-on-chip.

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M.L. Vieri ◽  
G. Greco ◽  
I. Tonazzini ◽  
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Jiangpo Zheng ◽  
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...  

Droplet streaming and nebulization on a shear horizontal surface acoustic wave device made of 36o Y-cut LiTaO3 have been reported. The streaming pattern inside the droplet is sensitive to the droplet shape, the position to the interdigital transducer, and the aperture size. Droplet nebulization was enhanced with the increased aperture size of the driven IDTs and mainly occurred in horizontal direction along two lateral sides of the droplet, which is perpendicular to the designed SAW propagation direction. The atomization duration increases with the droplet size and decreases with the driven power at a given droplet size. The maximum rate of the atomization is ~0.2 μL/s at an applied power of 6.7 W. This provides a simple and effective method of the integration of both bio-liquid sensing and fluid manipulation on a single substrate for lab-on-chip biosensing platform.


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