Diversity and assembly patterns of activated sludge microbial communities: A review

2018 ◽  
Vol 36 (4) ◽  
pp. 1038-1047 ◽  
Author(s):  
Yu Xia ◽  
Xianghua Wen ◽  
Bing Zhang ◽  
Yunfeng Yang
Keyword(s):  
Activated Sludge ◽  
Microbial Communities

scholarly journals Time-series genome-centric analysis unveils bacterial response to operational disturbance in activated sludge

2019 ◽  
Author(s):  
María Victoria Pérez ◽  
Leandro D. Guerrero ◽  
Esteban Orellana ◽  
Eva L. Figuerola ◽  
Leonardo Erijman
Keyword(s):  
Growth Rate ◽  
Time Series ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Operating Conditions ◽  
The Face

ABSTRACTUnderstanding ecosystem response to disturbances and identifying the most critical traits for the maintenance of ecosystem functioning are important goals for microbial community ecology. In this study, we used 16S rRNA amplicon sequencing and metagenomics to investigate the assembly of bacterial populations in a full-scale municipal activated sludge wastewater treatment plant over a period of three years, including a period of nine month of disturbance, characterized by short-term plant shutdowns. Following the reconstruction of 173 metagenome-assembled genomes, we assessed the functional potential, the number of rRNA gene operons and thein situgrowth rate of microorganisms present throughout the time series. Operational disturbances caused a significant decrease in bacteria with a single copy of the ribosomal RNA (rrn) operon. Despite only moderate differences in resource availability, replication rates were distributed uniformly throughout time, with no differences between disturbed and stable periods. We suggest that the length of the growth lag phase, rather than the growth rate, as the primary driver of selection under disturbed conditions. Thus, the system could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions.IMPORTANCEIn this work we investigated the response of microbial communities to disturbances in a full-scale activated sludge wastewater treatment plant over a time-scale that included periods of stability and disturbance. We performed a genome-wide analysis, which allowed us the direct estimation of specific cellular traits, including the rRNA operon copy number and the in situ growth rate of bacteria. This work builds upon recent efforts to incorporate growth efficiency for the understanding of the physiological and ecological processes shaping microbial communities in nature. We found evidence that would suggest that activated sludge could maintain its function in the face of disturbance by recruiting bacteria with the capacity to rapidly resume growth under unsteady operating conditions. This paper provides relevant insights into wastewater treatment process, and may also reveal a key role for growth traits in the adaptive response of bacteria to unsteady environmental conditions.

scholarly journals High temperature utilization of PAM and HPAM by microbial communities enriched from oilfield produced water and activated sludge

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Carolina Berdugo-Clavijo ◽  
Arindom Sen ◽  
Mojtaba Seyyedi ◽  
Harvey Quintero ◽  
Bill O’Neil ◽  
...  
Keyword(s):  
High Temperature ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Produced Water ◽  
Oilfield Produced Water

Evolution of microbial communities in the activated sludge process

1983 ◽  
Vol 17 (10) ◽  
pp. 1275-1279 ◽  
Author(s):  
Felix Kucnerowicz ◽  
Willy Verstraete
Keyword(s):  
Activated Sludge ◽  
Microbial Communities ◽  
Activated Sludge Process

Microbial population dynamics in laboratory-scale activated sludge reactors

2002 ◽  
Vol 46 (1-2) ◽  
pp. 19-27 ◽  
Author(s):  
K. Kaewpipat ◽  
C.P.L. Grady
Keyword(s):  
Activated Sludge ◽  
Microbial Communities ◽  
System Performance ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Treatment Plant ◽  
Laboratory Scale ◽  
Rrna Gene ◽  
Significant Finding ◽  
Prokaryotic Community ◽  
Activated Sludge Systems

As a first step in understanding nonlinear dynamics in activated sludge systems, two laboratory-scale sequencing batch reactors were operated under identical conditions and changes in their microbial communities were followed through microscopic examination, macroscopic observation, and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene segments from the prokaryotic community. Two experiments were performed. The first used activated sludge from a local wastewater treatment plant to start the replicate reactors. The second used the biomass from the first experiment as a source by intermixing the two and equally redistributing the biomass into the two replicate reactors. For both experiments, the two reactors behaved fairly similarly and had similar microbial communities for a period of 60 days following start-up. Beyond that, the microbial communities in the two reactors in the first experiment diverged in composition, while those in the second experiment remained fairly similar. This suggests that the degree of change occurring in replicate reactors depends upon the severity of perturbation to which they are exposed. The DGGE data showed that the bacterial communities in both experiments were highly dynamic, even though the system performance of the replicate reactors were very similar, suggesting that dynamics within the prokaryotic community is not necessarily reflected in system performance. Moreover, a significant finding from this study is that replicate activated sludge systems are not identical, although they can be very similar if started appropriately.

Effect of chemical oxygen demand load on the nitrification and microbial communities in activated sludge from an aerobic nitrifying reactor

2020 ◽  
Vol 66 (1) ◽  
pp. 59-70
Author(s):  
Dan Li ◽  
Xihong Liang ◽  
Zhengwei Li ◽  
Yao Jin ◽  
Rongqing Zhou ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Chemical Oxygen Demand ◽  
Microbial Communities ◽  
High Throughput Sequencing ◽  
Oxygen Demand ◽  
Nitrification Rate ◽  
Culture Time ◽  
Degradation Rates ◽  
Significant Difference ◽  
Over Time

In this study, we explored the effect of chemical oxygen demand (COD) load on the nitrification and microbial communities in activated sludge isolated from an aerobic nitrifying tank. The activated sludge was cultured in three different COD groups: L-COD, 200 mg/L; M-COD, 1200 mg/L; H-COD, 4200 mg/L. The results indicated that the COD exerts a negligible effect on the nitrogen removal ability within the first 24 h. However, the nitrification rate decreased with culture time; the ammonium degradation rates were found to be 80.26%, 57.56%, and 43.43% at 72 h in the three COD groups, respectively. These values correspond to decreases of 19.40%, 41.83%, and 51.48%, respectively, in relation to those observed at 24 h. The activated sludge in the different COD groups exhibited similar community compositions after 24 h, as assessed by Illumina high-throughput sequencing, while a significant difference in the relative abundances of some organisms occurred after 48 and 72 h. Proteobacteria was the main phylum, with a relative abundance of >51.45%. The genera Aridibacter, Paracoccus, Nitrospira, and Nitrosomonas were suppressed by COD load over time. This study may contribute to our knowledge about the nitrification ability and microbial communities in activated sludge at different COD load levels.

Sign in / Sign up

Export Citation Format

Share Document