Comparative chemical composition and antimicrobial activity study of essential oils from two imported lemon fruits samples against pathogenic bacteria

The study aimed to establish the chemical composition of fruit essential oils of M. gale and test their activities against the selected pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Acinetobacter baumannii), yeasts (Candida albicans, C. parapsilosis), fungi (Aspergillus fumigatus, A. flavus) and dermatophytes (Trichophyton rubrum, T. mentagrophytes). Fruit samples from natural (Western Lithuania) and anthropogenic (Eastern Lithuania) M. gale populations were studied separately. Essential oils were isolated from dried fruits by hydrodistillation and analysed by GC/FID and GC/MS methods; enantiomeric composition of α-pinene was established by chiral-phase capillary GC. Minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) of essential oils were determined using the broth microdilution method. Plants from the natural population with a humid marine climate accumulated significantly higher amounts of fruit essential oils (3.34±0.05%) than those from the anthropogenic population with a more continental climate (2.71±0.22%). In total, 39 volatiles including α-pinene (23.52–27.17%), 1,8-cineole (17.19–18.84%) and α-phellandrene (9.47–10.03%) as main compounds were identified. Chiral analysis demonstrated that (1S)-(–)-α-pinene prevailed over (1R)-(+)-α-pinene and amounted to 94.09–95.28% of all fraction of this monoterpene. The antimicrobial study in vitro indicated that C. parapsilosis, dermatophytes and Aspergillus fungi were more susceptible to fruit essential oils of M. gale, whereas E. coli and C. albicans were weakly inhibited even at the highest essential oil concentration. The strongest growth-inhibitory and bactericidal effect of sweet gale essential oil was established on S. aureus. This could be attributed to the major essential oil compounds with known antimicrobial activity, such as α-pinene, 1,8-cineole and a-phellandrene. Keywords: Myrica gale; essential oil; chemical compounds; terpenes; enantiomers; antimicrobial.

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Chemical Composition and Antimicrobial Activity of Cinnamon, Thyme, Oregano and Clove Essential Oils Against Plant Pathogenic Bacteria

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun201765041129 ◽

2017 ◽

Vol 65 (4) ◽

pp. 1129-1134 ◽

Cited By ~ 5

Author(s):

Matěj Božik ◽

Pavel Nový ◽

Pavel Klouček

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Antimicrobial Agents ◽

Antimicrobial Effect ◽

Fruit Trees ◽

Thymus Vulgaris ◽

Syzygium Aromaticum ◽

Plant Pathogenic Bacteria

Essential oils are volatile substances from plants and many of them have antimicrobial activity. For that reason, they have become known as a useful alternative to chemical preservatives and pesticides. In this study, we tested essential oils of four aromatic plants. Cinnamon (Cinnamomum zeylanicum), thyme (Thymus vulgaris), oregano (Origanum vulgare) and clove (Syzygium aromaticum) essential oils were investigated for their composition and antimicrobial effect against plant pathogenic bacteria (Pectobacterium spp. and Pseudomonas spp.). Both are commonly associated with diseased fruit trees in orchards and gardens. The chemical composition of the tested essential oils was identified by gas chromatography coupled with mass spectrometry. The cinnamon essential oil was most effective form tested oil. The experimental results indicated that the wild strains of tested bacteria are more resistant to essential oils than commonly used laboratory strains. In conclusion, certain essential oils could be used for the control of postharvest bacterial pathogens. The findings of the present study suggest that the essential oils have a potential to be used as antimicrobial agents.

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Antibacterial Activity and Chemical Composition of Essential Oils from Some Galium (Rubiaceae) Species Against Pathogenic Bacteria

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v5i11.1330-1333.1414 ◽

2017 ◽

Vol 5 (11) ◽

pp. 1330

Author(s):

Filiz Yağız ◽

Rifat Battaloğlu ◽

Sedef İlk ◽

Ahmet Savran

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Pseudomonas Syringae ◽

Pathogenic Bacteria ◽

Inhibitory Effect ◽

E Coli ◽

Gram Positive Bacteria ◽

Potent Inhibitory Effect

In this work, chemical composition and antimicrobial activity of the essential oils of Galium incanum, Galium dieckii ve Galium aladaghense were firstly reported. The essential oils were obtained from the all parts of the plant by hydrodistillation and analyzed by using GC-MS. Antimicrobial activity of synthezied essential oils was carried out against 5 pathogen bacteria Escherichia coli (E. coli) ATCC 25922, Staphylococcus aureus (S. aureus) ATCC 25923, Pseudomonas syringae pv. tomato (P. syringae) DC300, Salmonella enterica serotype Typhmurium (S. typhmurium) SL 1344 and Streptococcus mutans (S. mutans) ATCC 25175. According to the results, it was determined that isolated essential oils comprised of 61 compounds. Compounds of essential oils included that structure monoterpene (8.2%), monoterpenoid (14.75%) and sesquiterpene (14.75%). Unclassified compounds have been identified as other compounds. From the antimicrobial activity was observed that the isolated essential oil from Galium incanum, Galium dieckii ve Galium aladaghense exhibited a potent inhibitory effect against all gram negative and gram positive bacteria with diameter of inhibition zones ranging from 4.3 to 12.3 mm. Essential oil of Galium aladaghense indicated that high antimicrobial activity on all bacteria than Galium incanum and Galium dieckii.

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Chemical composition and antimicrobial activity of the essential oils of flowers, leaves and stems of Cotinus coggygria

Planta Medica ◽

10.1055/s-0028-1084931 ◽

2008 ◽

Vol 74 (09) ◽

Cited By ~ 3

Author(s):

T Milošević ◽

N Nićiforović ◽

V Mihailović ◽

S Solujić ◽

N Vuković

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Cotinus Coggygria

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Chemical composition and antimicrobial activity of essential oils of selected Apiaceous plants growing in Egypt

Planta Medica ◽

10.1055/s-0036-1596848 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

N Khalil ◽

M Ashour ◽

S Fikry ◽

A NaserSingab ◽

O Salama

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils

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Chemical composition and antimicrobial activity of the essential oils of Micromeria thymifolia, M. dalmatica and Satureja cuneifolia

Planta Medica ◽

10.1055/s-2007-986934 ◽

2007 ◽

Vol 73 (09) ◽

Cited By ~ 1

Author(s):

N Menković ◽

G Zdunić ◽

S Tasić ◽

M Ristić ◽

T Stević ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils

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Variability in chemical composition and antimicrobial activity of essential oil of Rosa × damascena Herrm. from mountainous regions of Iran

Chemical and Biological Technologies in Agriculture ◽

10.1186/s40538-021-00219-6 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Mansureh Ghavam ◽

Afsaneh Afzali ◽

Maria Manconi ◽

Gianluigi Bacchetta ◽

Maria Letizia Manca

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Environmental Conditions ◽

Raw Materials ◽

Rosa Damascena ◽

Mountainous Regions ◽

Aspergillus Brasiliensis ◽

Suitable Area

Abstract Background Essential oil of Rosa × damascena Herrm. is one of the most valuable and important raw materials for the flavor and fragrance industry. The cultivation of this plant has ancient origins, and Kashan was one of the first mountainous regions of Iran dealing with the cultivation of R. × damascena. In this study, both chemical composition and antimicrobial activity of different rose essential oils obtained from five mountainous areas of Kashan region (Maragh, Qamsar, Sadeh, Javinan, and Kamoo) has been investigated along with the influence of the environmental conditions on these properties. Results Results showed that yield and chemical composition of essential oils obtained from Rosa × damascena were significantly affected by the collection area. In particular, the yield of oils varied from ~0.08 to ~0.132% and citronellol (36.70-9.18%), geraniol (12.82-0.47%), nonadecane (22.73-10.36%), heneicosane (31.7-11.43%), and 1-nonadecene (6.03-3.93%) have been detected as main compounds in all the plants collected, but at different concentrations depending on the collection area. The best fragrance and the highest yield were found in the oil from Kamoo area. Similarly to the chemical composition, the antimicrobial activity of the essential oils was affected by their origin, and essential oil obtained from plants collected from Kamoo area disclosed the highest antibacterial and antifungal efficacy. Its inhibition halos were 17.33±0.58 mm against Aspergillus brasiliensis, 15.67±0.58 mm against Staphylococcus aureus, and 12.33±0. 58 mm against Streptococcus pyogenes. Essential oils of R. damascena were also effective against Gram-negative Pseudomonas aeruginosa and they had a MIC value of 62.50 μg/mL irrespective of the collection area (except the oil from Javinan area). On the contrary, the highest antifungal power against Candida albicans yeast was reached using the essential oil obtained from plants collected in Javinan region (MIC and MBC ~62.50 μg/mL). Conclusions Overall results underline the influence of environmental conditions of the different areas of Kashan region, on the chemical composition of and antimicrobial activity of the essential oils of Rosa × damascena. In addition, results disclosed that Kamoo seemed to be the most suitable area for the competitive cultivation of R. × damascena to the intensive production of aromatic flower oil and natural antimicrobial essential oils.

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