scholarly journals Antibacterial Activity and Chemical Composition of Essential Oils from Some Galium (Rubiaceae) Species Against Pathogenic Bacteria

2017 ◽  
Vol 5 (11) ◽  
pp. 1330
Author(s):  
Filiz Yağız ◽  
Rifat Battaloğlu ◽  
Sedef İlk ◽  
Ahmet Savran
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Potent Inhibitory Effect

In this work, chemical composition and antimicrobial activity of the essential oils of Galium incanum, Galium dieckii ve Galium aladaghense were firstly reported. The essential oils were obtained from the all parts of the plant by hydrodistillation and analyzed by using GC-MS. Antimicrobial activity of synthezied essential oils was carried out against 5 pathogen bacteria Escherichia coli (E. coli) ATCC 25922, Staphylococcus aureus (S. aureus) ATCC 25923, Pseudomonas syringae pv. tomato (P. syringae) DC300, Salmonella enterica serotype Typhmurium (S. typhmurium) SL 1344 and Streptococcus mutans (S. mutans) ATCC 25175. According to the results, it was determined that isolated essential oils comprised of 61 compounds. Compounds of essential oils included that structure monoterpene (8.2%), monoterpenoid (14.75%) and sesquiterpene (14.75%). Unclassified compounds have been identified as other compounds. From the antimicrobial activity was observed that the isolated essential oil from Galium incanum, Galium dieckii ve Galium aladaghense exhibited a potent inhibitory effect against all gram negative and gram positive bacteria with diameter of inhibition zones ranging from 4.3 to 12.3 mm. Essential oil of Galium aladaghense indicated that high antimicrobial activity on all bacteria than Galium incanum and Galium dieckii.

scholarly journals Comparison of the Chemical Composition and Antimicrobial Activity of Thymus serpyllum Essential Oils

2015 ◽  
Vol 43 (2) ◽  
pp. 432-438 ◽  
Author(s):  
Aneta WESOŁOWSKA ◽  
Monika GRZESZCZUK ◽  
Dorota JADCZAK ◽  
Paweł NAWROTEK ◽  
Magdalena STRUK
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Gas Chromatography Mass Spectrometry ◽  
E Coli ◽  
Thymus Serpyllum ◽  
Main Components ◽  
Total Oil

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.

scholarly journals Chemical Composition and Antimicrobial Activity of the Essential Oils of Leaves, Roots and Bark of Glossocalyx staudtii

2009 ◽  
Vol 4 (8) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Huguette Agnaniet ◽  
Thomas Makani ◽  
Raphaël Bikanga ◽  
Louis Clément Obame ◽  
Jacques Lebibi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Disc Diffusion ◽  
Aliphatic Compounds ◽  
Gram Positive Bacteria ◽  
Agar Disc ◽  
Leaf Oil ◽  
Main Component

The chemical composition of the essential oils obtained by hydrodistillation from air dried leaves, bark and roots of Glossocalyx staudtii Engl. grown in Gabon was analyzed by GC and GC-MS. The essential oil content was 0.84% (w/w), 0.28% (w/w), and 0.74% (w/w), respectively. The leaf oil was characterized by a high content of monoterpene hydrocarbons (64.8%), with β-pinene (30.6%) and α-pinene (22.6%) as the major constituents. The oil obtained from the bark contained 30.5% of oxygenated monoterpenes with cryptone as the main constituent (11.5%) and 28.9% of aliphatic compounds, with 9.3% of 2-tridecanone; in the roots oil, the main component was 2-tridecanone (55.2%). The antimicrobial activity of the oils was studied by means of the agar disc diffusion and broth microdilution methods. The Gram positive bacteria were the most sensitive to the essential oils. A significant anticandidal effect of the bark essential oil was observed.

scholarly journals Chemical composition and antimicrobial activity of fruit essential oils of Myrica gale, a neglected non-wood forest product

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Kristina Ložienė ◽  
Juozas Labokas ◽  
Vaida Vaičiulytė ◽  
Jurgita Švedienė ◽  
Vita Raudonienė ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Chiral Phase ◽  
Myrica Gale ◽  
Broth Microdilution Method ◽  
Fruit Samples

The study aimed to establish the chemical composition of fruit essential oils of M. gale and test their activities against the selected pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Acinetobacter baumannii), yeasts (Candida albicans, C. parapsilosis), fungi (Aspergillus fumigatus, A. flavus) and dermatophytes (Trichophyton rubrum, T. mentagrophytes). Fruit samples from natural (Western Lithuania) and anthropogenic (Eastern Lithuania) M. gale populations were studied separately. Essential oils were isolated from dried fruits by hydrodistillation and analysed by GC/FID and GC/MS methods; enantiomeric composition of α-pinene was established by chiral-phase capillary GC. Minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) of essential oils were determined using the broth microdilution method. Plants from the natural population with a humid marine climate accumulated significantly higher amounts of fruit essential oils (3.34±0.05%) than those from the anthropogenic population with a more continental climate (2.71±0.22%). In total, 39 volatiles including α-pinene (23.52–27.17%), 1,8-cineole (17.19–18.84%) and α-phellandrene (9.47–10.03%) as main compounds were identified. Chiral analysis demonstrated that (1S)-(–)-α-pinene prevailed over (1R)-(+)-α-pinene and amounted to 94.09–95.28% of all fraction of this monoterpene. The antimicrobial study in vitro indicated that C. parapsilosis, dermatophytes and Aspergillus fungi were more susceptible to fruit essential oils of M. gale, whereas E. coli and C. albicans were weakly inhibited even at the highest essential oil concentration. The strongest growth-inhibitory and bactericidal effect of sweet gale essential oil was established on S. aureus. This could be attributed to the major essential oil compounds with known antimicrobial activity, such as α-pinene, 1,8-cineole and a-phellandrene. Keywords: Myrica gale; essential oil; chemical compounds; terpenes; enantiomers; antimicrobial.

scholarly journals Essential oils of Thymus pulegioides and Thymus glabrescens from Romania: Chemical composition and antimicrobial activity

2010 ◽  
Vol 75 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Mariana Pavel ◽  
Mihailo Ristic ◽  
Tatjana Stevic
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Antimicrobial Properties ◽  
Herbal Drug ◽  
Qualitative And Quantitative Analysis ◽  
Gram Positive Bacteria ◽  
Main Components

The aim of this work was to analyse the chemical composition and antimicrobial properties of essential oils isolated from two wild-growing species of thyme (Thymus pulegioides L. and T. glabrescens Willd.) originating from different locations in Romania. The yield of essential oil was determined according to European Pharmacopoeia standards. Qualitative and quantitative analysis of the oils was performed using GC and GC/MS. The antimicrobial activity was tested by the microdilution technique against Gram-negative and Gram-positive bacteria (Escherichia coli, Salmonella typhimurium, S. enteritisdis, Enterobacter cloacae, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, S. epidermidis, Streptococcus faecalis, Bacillus subtilis, Micrococcus luteus, M. flavus and Listeria monocytogenes) and human pathogen yeast Candida albicans. The essential oil of Thymus pulegioides was obtained in a yield of 0.7-1 % (v/d.w. herbal drug) and the main components were carvacrol (50.5-62.6 %), ?-terpinene (9.8-9.9 %) and p-cymene (5.8-7.1 %). The essential oil of T. glabrescens was obtained in a yield of 0.7 (v/d.w. herbal drug) and the main components were geraniol (55.5 %), neryl acetate (11.1 %) and ?-bisabolene (6.7 %). The essential oils inhibited microbial growth at concentrations of 10.8-27 ?l/ml.

scholarly journals Antimicrobial Activity of Selected Essential Oils against Selected Pathogenic Bacteria: In Vitro Study

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 546
Author(s):  
Nikola Puvača ◽  
Jovana Milenković ◽  
Tamara Galonja Galonja Coghill ◽  
Vojislava Bursić ◽  
Aleksandra Petrović ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Tea Tree ◽  
E Coli

The worldwide problem of infectious diseases has appeared in recent years, and antimicrobial agents are crucial in reducing disease emergence. Nevertheless, the development and distribution of multidrug-resistant (MDR) strains in pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, Salmonella Typhi and Citrobacter koseri, has become a major society health hazard. Essential oils could serve as a promising tool as a natural drug in fighting the problem with these bacteria. The current study aimed to investigate the antimicrobial effectiveness of tea tree (Melaleuca alternifolia (Maiden and Betche) Cheel), rosemary (Rosmarinus officinalis L.), eucalyptus (Eucalyptus obliqua L’Hér.), and lavender (Lavandula angustifolia Mill) essential oils. The antimicrobial properties of essential oils were screened against four pathogenic bacteria, E. coli, S. aureus, S. Tyhpi, and C. koseri, and two reference bacterial strains, while for the testing, the agar well diffusion method was used. Gas chromatography (GC) and gas chromatography–mass spectrometric (GC–MSD) analyses were performed on essential oils. The obtained results showed that M. alternifolia essential oil is the richest in terpinen-4-ol, R. officinalis and E. oblique essential oils in 1,8-cineole, and L. angustifolia essential oil in α-terpinyl acetate. In addition, the main bioactive compounds present in the essential oil of tea tree are rich in α-pinene (18.38%), limonene (7.55%) and γ-terpinene (14.01%). The essential oil of rosemary is rich in α-pinene (8.38%) and limonene (11.86%); eucalyptus essential oil has significant concentrations of α-pinene (12.60%), p-cymene (3.24%), limonene (3.87%), and γ-terpinene (7.37%), while the essential oil of lavender is rich in linalool (10.71%), linalool acetate (9.60%), α-terpinyl acetate (10.93%), and carbitol (13.05%) bioactive compounds, respectively. The obtained results from the in vitro study revealed that most of the essential oils exhibited antimicrobial properties. Among the tested essential oils, tea tree was discovered to demonstrate the strongest antimicrobial activity. The recorded MIC of S. Typhi was 6.2 mg/mL, 3.4 mg/mL of C. koseri, 3.1 mg/mL of E. coli, and 2.7 mg/mL of E. Coli ATCC 25922, compared to M. alternifolia. Similarly, only S. aureus ATCC 25923 showed antimicrobial activity towards R. officinalis (1.4 mg/mL), E. oblique (2.9 mg/mL), and L. angustifolia (2.1 mg/mL). Based on the obtained results, it is possible to conclude that tea tree essential oil might be used as an ecological antimicrobial in treating infectious diseases caused by the tested pathogens.

scholarly journals Chemical composition and antimicrobial activity of Pinus halepensis from Algeria

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Messaoud Ramdani ◽  
RIMA HAICHOUR1 ◽  
TAKIA LOGRADA ◽  
PIERRE CHALARD ◽  
GILLES FIGUEREDO4
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Pinus Halepensis ◽  
Antibacterial Activities ◽  
Klebsiella Pneumonia ◽  
E Coli ◽  
Aerial Parts ◽  
Methyl Butanoate

Abstract. Haichour R, Lograda T, Ramdani M, Chalard P, Figueredo G. 2020. Chemical composition and antimicrobial activity of Pinus halepensis from Algeria. Biodiversitas 21: 4345-4360. The chemical composition of Pinus halepensis essential oils and their antibacterial activities were investigated. Plant samples were collected in the flowering stage from the East locality of Algeria. The aerial parts of P. halepensis obtained from fifteen localities were subjected to a hydro-distillation, providing pale yellow viscous oils. The obtained yield average was 0.64 ± 0.37%. The analysis of the chemical composition of essential oils was performed by using GC and GC / MS. A total of thirty-seven compounds representing 99.4 ± 0.5% of the total oils were identified in P. halepensis. The hydrocarbon compounds were found to dominate essential oils in P. halepensis. In addition, the major compounds were caryophyllene-E (29.06 ± 8.5%), myrcene (19.14 ± 6.67%), a-pinene (16.86 ± 3.35%), phenyl ethyl 3-methyl butanoate (5.67 ± 2.47%), a-humulene (4.81 ± 1.43%), terpinolene (3.94 ± 1.6%) and sabinene (3.11 ± 1.84%). Based on their chemical composition, essential oil samples were clustered in five groups by UPGMA analysis. The first group includes the Tafrent essential oil population. It is characterized by the phenyl ethyl-3-methyl butanoate – Sabinene ‒ Δ3-carene chemotype. The second group was subdivided into two clusters. The first cluster or chemotype was characterized by the myrcene ‒ α-pinene ‒ caryophyllene-E. The second chemotype was characterized by caryophyllene-E ‒ α-humulene ‒ myrcene. The essential oil of P. halepensis is an effective antimicrobial against Staphylococcus aureus; Klebsiella pneumonia; Salmonella enteric spp arizonae; Listeria innocua; Proteus mirabilis and Bacillus cereus, and no effect on the E. coli and Pseudomonas aeruginosa.

scholarly journals Variability in chemical composition and antimicrobial activity of essential oil of Rosa × damascena Herrm. from mountainous regions of Iran

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Mansureh Ghavam ◽  
Afsaneh Afzali ◽  
Maria Manconi ◽  
Gianluigi Bacchetta ◽  
Maria Letizia Manca
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Environmental Conditions ◽  
Raw Materials ◽  
Rosa Damascena ◽  
Mountainous Regions ◽  
Aspergillus Brasiliensis ◽  
Suitable Area

Abstract Background Essential oil of Rosa × damascena Herrm. is one of the most valuable and important raw materials for the flavor and fragrance industry. The cultivation of this plant has ancient origins, and Kashan was one of the first mountainous regions of Iran dealing with the cultivation of R. × damascena. In this study, both chemical composition and antimicrobial activity of different rose essential oils obtained from five mountainous areas of Kashan region (Maragh, Qamsar, Sadeh, Javinan, and Kamoo) has been investigated along with the influence of the environmental conditions on these properties. Results Results showed that yield and chemical composition of essential oils obtained from Rosa × damascena were significantly affected by the collection area. In particular, the yield of oils varied from ~0.08 to ~0.132% and citronellol (36.70-9.18%), geraniol (12.82-0.47%), nonadecane (22.73-10.36%), heneicosane (31.7-11.43%), and 1-nonadecene (6.03-3.93%) have been detected as main compounds in all the plants collected, but at different concentrations depending on the collection area. The best fragrance and the highest yield were found in the oil from Kamoo area. Similarly to the chemical composition, the antimicrobial activity of the essential oils was affected by their origin, and essential oil obtained from plants collected from Kamoo area disclosed the highest antibacterial and antifungal efficacy. Its inhibition halos were 17.33±0.58 mm against Aspergillus brasiliensis, 15.67±0.58 mm against Staphylococcus aureus, and 12.33±0. 58 mm against Streptococcus pyogenes. Essential oils of R. damascena were also effective against Gram-negative Pseudomonas aeruginosa and they had a MIC value of 62.50 μg/mL irrespective of the collection area (except the oil from Javinan area). On the contrary, the highest antifungal power against Candida albicans yeast was reached using the essential oil obtained from plants collected in Javinan region (MIC and MBC ~62.50 μg/mL). Conclusions Overall results underline the influence of environmental conditions of the different areas of Kashan region, on the chemical composition of and antimicrobial activity of the essential oils of Rosa × damascena. In addition, results disclosed that Kamoo seemed to be the most suitable area for the competitive cultivation of R. × damascena to the intensive production of aromatic flower oil and natural antimicrobial essential oils.

scholarly journals Evaluation of the Antimicrobial Activity of Some Components of the Essential Oils of Plants Used in the Traditional Medicine of the Tehuacán-Cuicatlán Valley, Puebla, México

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 295
Author(s):  
Sebastián Candelaria-Dueñas ◽  
Rocío Serrano-Parrales ◽  
Marisol Ávila-Romero ◽  
Samuel Meraz-Martínez ◽  
Julieta Orozco-Martínez ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Qualitative Evaluation ◽  
Bactericidal Effect ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Diffusion Technique ◽  
Agar Dilution

In Tehuacán-Cuicatlán valley (Mexico), studies have been carried out on the essential oils of medicinal plants with antimicrobial activity and it was found that they present compounds in common such as: α-pinene, β-pinene, carvacrol, eugenol, limonene, myrcene, ocimene, cineole, methyl salicylate, farnesene, and thymol. The goal of this study was to assess the antimicrobial activity of essential oils’ compounds. The qualitative evaluation was carried out by the Kirby Baüer agar diffusion technique in Gram-positive bacteria (11 strains), Gram-negative bacteria (18 strains), and yeasts (8 strains). For the determination of the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), the agar dilution method was used. All the evaluated compounds presented antimicrobial activity. The compounds eugenol and carvacrol showed the largest inhibition zones. Regarding yeasts, the compounds ocimene, cineole, and farnesene did not show any activity. The compounds eugenol, carvacrol, and thymol presented the lowest MIC; bactericidal effect was observed at MIC level for S. aureus 75MR, E. coli 128 MR, and C albicans CUSI, for different compounds, eugenol, carvacrol, and thymol. Finally, this study shows that the essential oils of plants used by the population of Tehuacán-Cuicatlán valley share compounds and some of them have antibacterial and fungicidal activity.

scholarly journals Chemical Composition and Antimicrobial Activity of the Essential oils of 14 known Ficus species – A Concise Review

2021 ◽  
Vol 12 (6) ◽  
pp. 8003-8034
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Volatile Oils ◽  
Good Source ◽  
Concise Review ◽  
Living Organisms

Compounds useful for drugs, cosmetics, and food have been obtained directly or indirectly from living organisms over the years. However, there has been a renewed interest in getting useful compounds from living organisms, especially plants. Essential oils, interchangeably called volatile oils, are bioactive compounds found in minute quantities in some plants. Essential or volatile oils have been known for years to find usefulness in foods, drugs (antimicrobial, antifungal), and cosmetics. This review attempts to summarize information on the essential oil from Ficus species concerning their morphology, pharmacology, bioactivity, and application. This was achieved by gathering information on essential oils from different Ficus species. Essential oils from Ficus species are a good source of bioactive compounds for use in drug, food, and cosmetic industries. It is worthy to note that Nigerian Figs were characterized by the high presence of phytol and 6,10,14-trimethyl-2-pentadecanone, and these compounds are, therefore, seen as markers. Furthermore, this review presents numerous insights on how to best harness the different potentials of the essential oils and possibilities to be examined.

scholarly journals Chemical Composition and Antifungal Activity of the Essential Oils of Schinus weinmannifolius Collected in the Spring and Winter

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900
Author(s):  
Camila Hernandes ◽  
Silvia H. Taleb-Contini ◽  
Ana Carolina D. Bartolomeu ◽  
Bianca W. Bertoni ◽  
Suzelei C. França ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antimicrobial Action ◽  
Pharmacological Profile

Reports on the chemical and pharmacological profile of the essential oil of Schinus weinmannifolius do not exist, although other Schinus species have been widely investigated for their biological activities. This work aimed to evaluate the chemical composition and antimicrobial activity of the essential oil of S. weinmannifolius collected in the spring and winter. The essential oils were extracted by hydrodistillation, analyzed by GC/MS and submitted to microdilution tests, to determine the minimum inhibitory concentration. The oils displayed different chemical composition and antimicrobial action. Bicyclogermacrene and limonene predominated in the oils extracted in the winter and spring, respectively, whereas only the latter oil exhibited antifungal activity.

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