scholarly journals Chemical Composition and Antimicrobial Activity of Cinnamon, Thyme, Oregano and Clove Essential Oils Against Plant Pathogenic Bacteria

2017 ◽  
Vol 65 (4) ◽  
pp. 1129-1134 ◽  
Author(s):  
Matěj Božik ◽  
Pavel Nový ◽  
Pavel Klouček
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Antimicrobial Effect ◽  
Fruit Trees ◽  
Thymus Vulgaris ◽  
Syzygium Aromaticum ◽  
Plant Pathogenic Bacteria

Essential oils are volatile substances from plants and many of them have antimicrobial activity. For that reason, they have become known as a useful alternative to chemical preservatives and pesticides. In this study, we tested essential oils of four aromatic plants. Cinnamon (Cinnamomum zeylanicum), thyme (Thymus vulgaris), oregano (Origanum vulgare) and clove (Syzygium aromaticum) essential oils were investigated for their composition and antimicrobial effect against plant pathogenic bacteria (Pectobacterium spp. and Pseudomonas spp.). Both are commonly associated with diseased fruit trees in orchards and gardens. The chemical composition of the tested essential oils was identified by gas chromatography coupled with mass spectrometry. The cinnamon essential oil was most effective form tested oil. The experimental results indicated that the wild strains of tested bacteria are more resistant to essential oils than commonly used laboratory strains. In conclusion, certain essential oils could be used for the control of postharvest bacterial pathogens. The findings of the present study suggest that the essential oils have a potential to be used as antimicrobial agents.

THE ACTIVITY OF ESSENTIAL OILS OBTAINED FROM SPECIES AND INTERSPECIES HYBRIDS OF THE Mentha GENUS AGAINST SELECTED PLANT PATHOGENIC BACTERIA

2018 ◽  
Vol 17 (6) ◽  
pp. 167-174 ◽  
Author(s):  
Małgorzata Schollenberger ◽  
Tomasz M. Staniek ◽  
Elżbieta Paduch-Cichal ◽  
Beata Dasiewicz ◽  
Agnieszka Gadomska-Gajadhur ◽  
...  
Keyword(s):  
Essential Oils ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Mentha Piperita ◽  
Mentha Spicata ◽  
Plant Essential Oils ◽  
Plant Pathogenic Bacteria ◽  
Interspecies Hybrids

Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on agar plates filled with the target organism. All essential oils screened exhibited a higher level of antibacterial activity against A. tumefaciens and X. arboricola pv. corylina than streptomycin used as a standard in all tests. The antimicrobial effect of streptomycin and five mint oils was at the same level for P. syringae pv. syringae. There were no significant differences in the influence of the chocolate mint oil on the growth inhibition of all bacteria tested. Plant essential oils from pineapple mint, apple mint, spearmint and strawberry mint showed the weakest antimicrobial activity against P. syringae pv. syringae and the strongest towards A. tumefaciens and X. arboricola pv. corylina. The essential oils from strawberry mint, pineapple mint, spearmint and apple mint had the strongest effect on A. tumefaciens, and the lowest inhibitory activity was exhibited by the chocolate mint and orange mint essential oils. X. arboricola pv. corylina was the most sensitive to the strawberry mint, pineapple mint and spearmint oils. The chocolate mint oil showed the greatest activity against P. syringae pv. syringae.

scholarly journals Essential Oils of Aromatic Plants with Antibacterial, Anti-Biofilm and Anti-Quorum Sensing Activities against Pathogenic Bacteria

Antibiotics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 147 ◽  
Author(s):  
Marlon Cáceres ◽  
William Hidalgo ◽  
Elena Stashenko ◽  
Rodrigo Torres ◽  
Claudia Ortiz
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Quorum Sensing ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Thymus Vulgaris ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Biofilm Inhibition

Both the ability of bacteria to form biofilms and communicate through quorum sensing allows them to develop different survival or virulence traits that lead to increased bacterial resistance against conventional antibiotic therapy. Here, seventeen essential oils (EOs) were investigated for the antimicrobial, antibiofilm, and anti-quorum sensing activities on Escherichia. coli O157:H7, Escherichia coli O33, and Staphylococcus epidermidis ATCC 12228. All essential oils were isolated from plant material by using hydrodistillation and analyzed by GC-MS. The antimicrobial activity was performed by using the microdilution technique. Subinhibitory concentrations of each EO were assayed for biofilm inhibition in both bacterial strains. Quantification of violacein in Chromobacterium violaceum CV026 was performed for the anti-quorum sensing activity. The cytotoxicity activity of the EOs was evaluated on Vero cell line by using MTT method. Thymol-carvacrol-chemotype (I and II) oils from Lippia origanoides and Thymus vulgaris oil exhibited the higher antimicrobial activity with MIC values of 0.37–0.75 mg/mL. In addition, these EOs strongly inhibited the biofilm formation and violacein (QS) production in a concentration-dependent manner, highlighting thymol-carvacrol-chemotype (II) oil as the best candidate for further studies in antibiotic design and development against bacterial resistance.

Research on Antimicrobial Activity of Functional Protein Films

2013 ◽  
Vol 785-786 ◽  
pp. 660-665 ◽  
Author(s):  
Zhi Ying Huang ◽  
Qiao Lei ◽  
Jian Qiang Bao ◽  
Qian Nan Xun
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Antimicrobial Effect ◽  
Potassium Sorbate ◽  
Protein Films ◽  
Functional Protein ◽  
Elongation At Break ◽  
Mechanical And Physical Properties

Antimicrobial effect of functional protein films incorporating garlic oil (GO), potassium sorbate (PS) and nisin (N) at various concentrations were discussed. This activity was tested against food pathogenic bacteria namely Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus).Mechanical and physical properties were characterized. In the range of antimicrobial agents concentration studied, tensile strength (TS) and elongation at break (E) of functional protein films were changed by incorporating GO,PS and N. And the WVP value of functional protein films decreased as antimicrobial agents added. GO incorporated into protein films had no effect on E.coli, but incorporation of GO at 300μl had antimicrobial activity against S.aureus. Protein films incorporated with PS showed antimicrobial activity against S.aureus, but there was no effect on E.coli. Incorporation of N at the lowest level of 25,000 IU had antimicrobial activity against both E.coli and S.aureus.

scholarly journals Monitoring of Antimicrobial Activity of Essential Oils Using Molecular Markers

2009 ◽  
Vol 3 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Hend A. Hamedo
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Random Amplified Polymorphic Dna ◽  
Antimicrobial Effect ◽  
E Coli ◽  
Specific Effects ◽  
And Staphylococcus Aureus ◽  
Rosemary Essential Oil

Technological application of essential oils, as natural antimicrobial agents, to reduce the effect of pathogenic microorganisms, requires new methods of detection. The present work evaluated the parameters of antimicrobial activity of the essential oils of rosemary (Rosmarinus officinalis) on two pathogenic strains Escherichia coli and Staphylococcus aureus. The MBC and MIC values were of 2.5, 25 μl ml-1, and values of 1.25 and 5 μl ml-1 for the two strains respectively. In this study, an attempt has been made to evaluate randomly amplified polymorphic DNA (RAPD) analysis for its potential to establish antimicrobial effect of rosemary essential oil. For the preliminary assessment, this study compared the effects occurring at molecular levels in E. coli and Staph. aureus exposed to rosemary essential oil at the MIC concentrations for the two organisms. The qualitative modifications arising in random amplified polymorphic DNA (RAPD) profiles as a measure of DNA effects were compared with control which showed many differences. In conclusion, the measurement of parameters at molecular levels is valuable for investigating the specific effects of agents interacting with DNA.

scholarly journals Determination of Antimicrobial Activity of Different Essential Oils Obtained from Plants on Staphylococcus aureus Strains Isolated from Foods

2020 ◽  
Vol 8 (4) ◽  
pp. 1012-1017 ◽  
Author(s):  
Hacer Aslan Canberi ◽  
Esra Şentürk ◽  
Simge Aktop ◽  
Pınar Şanlıbaba
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Thyme Oil ◽  
Staphyloccocus Aureus ◽  
Hypericum Perforatum L ◽  
Different Strains

Essential oils (EOs) are known for its antimicrobial activity against several pathogenic bacteria. The present work evaluated the antimicrobial activity of 15 different EOs on survival of different strains of different Staphyloccocus aureus strains isolated from traditional cheeses by disc diffusion method. The most antimicrobial activity on the strains was found as oil thyme oil (mean zone diameter 23.203 mm). Clove oil and black seed oil had the highest antimicrobial activity after thyme oil with average zone diameters of 13.698 mm and 11.267 mm respectively. Hypericum perforatum L. oil (mean zone 6.209 mm), ginger oil (mean zone 6.250 mm) and garlic oil (mean zone 6.267 mm) were the lowest antimicrobial activity. New studies about antimicrobial effect of EOs in vivo conditions are recommended.

scholarly journals Evaluation of antimicrobial activity of three important medicinal plants of South India

2019 ◽  
Vol 8 (1) ◽  
pp. 3484
Author(s):  
Gopalakrishnaiah B. ◽  
Aniel Kumar O.
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Development Program ◽  
Antimicrobial Effect ◽  
Trichophyton Mentagrophytes ◽  
Crude Extracts ◽  
The World

India is found to be a country with rich biodiversity and enormous treasure of herbal plants and consequently called as medicinal garden of the world. Plants are the richest source of natural antimicrobial agents. In recent years drug resistance to human pathogenic bacteria has been commonly reported from all over the world. Therefore, there is a need to develop alternative antimicrobial drugs for the treatment of infectious diseases; one approach is to screen local medicinal plants for possible antimicrobial properties. The present study was designed to evaluate the antimicrobial efficacy of hexane, chloroform and methanol crude extracts of the leaves of three important medicinal plants viz., Biophytum sensitivum (L.) DC, Bougainvillea spectabilis L. and Caesalpinia bonducella (L.) Fleming, collected from in and around Visakhapatnam District. The antimicrobial activity of the crude extracts was tested against three Gram Positive bacteria (Bacillus subtilis MTCC 441, Enterococcus faecalis MTCC 439, Staphylococcus aureus MTCC 737), Three Gram Negative bacteria (Escherichia coli MTCC 443, Proteus vulgaris MTCC 426 and Pseudomonas aeruginosa MTCC 1688) and three Fungal strains (Candida albicans MTCC 227, Epidermophyton floccosum MTCC 613 and Trichophyton mentagrophytes MTCC 7687) using agar well diffusion assay. Our results demonstrated that methanol extracts of these plants leaves have concentration dependent antibacterial activity against some of the tested organisms. Further studies should be undertaken to elucidate the exact mechanism of action of antimicrobial effect to identify the active ingredients which can be used for drug development program.

Antimicrobial activity of essential oils against foodborne multidrug-resistant enterococci and aeromonads in planktonic and biofilm state

2018 ◽  
Vol 25 (2) ◽  
pp. 101-108 ◽  
Author(s):  
Ana P Quendera ◽  
António S Barreto ◽  
Teresa Semedo-Lemsaddek
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Foodborne Pathogens ◽  
Antimicrobial Effect ◽  
Multidrug Resistant ◽  
Thymus Vulgaris ◽  
Cymbopogon Flexuosus ◽  
Subsequent Determination ◽  
Colony Counting ◽  
Enterococcus Spp

The selection and use of natural compounds with antimicrobial activity against foodborne pathogens is of major importance. The present study evaluated the antimicrobial activity of commercial essential oils against multidrug-resistant Enterococcus spp. and Aeromonas spp. Cymbopogon flexuosus and Thymus vulgaris essential oils presented the highest inhibitory zones against both bacterial groups ( p < 0.05). Subsequent determination of the minimum inhibitory concentrations showed values between 0.47 and 1.9 mg/ml for Aeromonas spp. and from 1.9 to 15 mg/ml for Enterococcus spp. The antimicrobial effect of C. flexuosus and T. vulgaris essential oils was also assessed against biofilms. Bacteria in biofilm state were subjected to 30 min or 1 h of exposure to each essential oil and eradication ability estimated by colony counting. Both essential oils exhibited antimicrobial activity against preformed Aeromonas biofilms, but were unable to successfully eradicate biofilms produced by enterococci, in the conditions under investigation. Nonetheless, the presumptive use of essential oils in the food industry should be considered in further investigations.

scholarly journals IN VITRO ANTIMICROBIAL ACTIVITY OF SOME ESSENTIAL OILS AGAINST BACTERIAL PATHOGENS CAUSING SKIN DISEASES IN VAPOR PHASE

2019 ◽  
pp. 113-115
Author(s):  
PURIT PATTANAPANIT ◽  
SUNISA MITHONGLANG ◽  
SUNITA MITHONGLANG ◽  
SURACHAI TECHAOEI
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Syzygium Aromaticum ◽  
Aromatic Plants ◽  
Volatile Oils

Objective: The objective of this study was to evaluate the antimicrobial activity of volatile oils from aromatic plants against pathogenic bacteria.Methods: Thai aromatic plants such as Pogostemon cablin (Blanco) Benth (Patchouli oil), Cymbopogon nardus Rendle (Citronella grass oil), Pelargoniumroseum (Geranium oil), Syzygium aromaticum (L.) Merrill and Perry (clove oil), Cinnamomum spp.(cinnamon oil), and Cymbopogon citratus (DC.) Stapf.(lemongrass oil) were selected. Essential oils were obtained by water distillation and were stored at 4°C until use. Five human pathogenic bacteria wereobtained from Thai traditional Medicine College, Rajamangala University of Technology, Staphylococcus epidermidis, Escherichia coli, Staphylococcusaureus, methicillin-resistant S. aureus (MRSA), and Pseudomonas aeruginosa. The antibacterial activity of volatile oils was determined by disc-diffusionassay. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each essential oil were determined.Results: Our study showed that 10% of essential oil from Cinnamomum spp. was the most potential against S. aureus, MRSA, and E. coli when assayedby disc-diffusion method with inhibition zones ranging from 37.66±0.57 to 45.33±1.15 mm and from 29.33±0.57 to 36.00±1.00 for lemongrass oilwith MIC and MBC of 1.25%.Conclusion: From this study, it can be concluded that some essential oils have potential antibacterial activity. The present investigation providessupport to the antibacterial properties of essential oils and will be applied to health-care product as aroma antibacterial products.

scholarly journals Chemical Composition and Antimicrobial Activities of the Essential Oils from Ocimum Selloi and Hesperozygis myrtoides

2011 ◽  
Vol 6 (7) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Márcia G. Martini ◽  
Humberto R. Bizzo ◽  
Davyson de L. Moreira ◽  
Paulo M. Neufeld ◽  
Simone N. Miranda ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Open Air Markets ◽  
Janeiro State

Ocimum selloi, a traditional medicinal plant from Brazil, is sold in open-air markets at Rio de Janeiro State. Hesperozygis myrtoides is a very aromatic small bush found in the State of Minas Gerais, Brazil, growing at an altitude of 1800m. The chemical composition of both essential oils was analyzed as well as their antimicrobial activity against fungi and bacteria. For all specimens of Ocimum selloi obtained at open-air markets, methylchavicol was major compound found (93.6% to 97.6%) in their essential oils. The major compounds identified in the oil of H. myrtoides were pulegone (44.4%), isomenthone (32.7%), and limonene (3.5%). Both oils displayed antimicrobial activity against all tested microorganisms but Candida albicans was the most susceptible one. Combinations of the two oils in different proportions were tested to verify their antimicrobial effect against C. albicans, which, however, was not modified in any of the concentrations tested. The minimum inhibitory concentration (MIC) was determined to confirm the antimicrobial activity against C. albicans as well as other clinical isolates ( C. glabrata, C. krusei, C. parapsilosis and C. tropicalis).

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