scholarly journals Gene expression signatures of a fibroblastoid preosteoblast and cuboidal osteoblast cell model compared to the MLO-Y4 osteocyte cell model

Bone ◽  
2009 ◽  
Vol 44 (1) ◽  
pp. 32-45 ◽  
Author(s):  
Wuchen Yang ◽  
Marie A. Harris ◽  
Jelica Gluhak Heinrich ◽  
Dayong Guo ◽  
Lynda F. Bonewald ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Model ◽  
Osteoblast Cell ◽  
Gene Expression Signatures

scholarly journals The Role of Semipurified Fractions Isolated from Quercus infectoria on Bone Metabolism by Using hFOB 1.19 Human Fetal Osteoblast Cell Model

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Amira Raudhah Abdullah ◽  
Hermizi Hapidin ◽  
Hasmah Abdullah
Keyword(s):  
Gene Expression ◽  
Bone Formation ◽  
Column Chromatography ◽  
Cell Model ◽  
Osteoblast Cell ◽  
Isolation And Purification ◽  
Traditional Medicines ◽  
Phytochemical Compounds ◽  
Layer Chromatography ◽  
Quercus Infectoria

Background. Quercus infectoria (QI) is a plant used in traditional medicines in Asia. The plant was reported to contain various active phytochemical compounds that have potential to stimulate bone formation. However, the precise mechanism of the stimulation effect of QI on osteoblast has not been elucidated. The present study was carried out to isolate QI semipurified fractions from aqueous QI extract and to delineate the molecular mechanism of QI semipurified fraction that enhanced bone formation by using hFOB1.19 human fetal osteoblast cell model. Methods. Isolation of QI semipurified fractions was established by means of column chromatography and thin layer chromatography. Established QI semipurified fractions were identified using Liquid Chromatography-Mass Spectrometry (LC-MS). Cells were treated with derived QI semipurified fractions and investigated for mineralization deposition and protein expression level of BMP-2, Runx2, and OPN by ELISA followed gene expression analysis of BMP-2 and Runx2 by RT-PCR. Results. Column chromatography isolation and purification yield Fractions A, B, and C. LC-MS analysis reveals the presence of polyphenols in each fraction. Results show that QI semipurified fractions increased the activity and upregulated the gene expression of BMP-2 and Runx2 at day 1, day 3, and day 7. OPN activity increased in cells treated with QI semipurified fractions at day 1 and day 3. Meanwhile, at day 7, expression of OPN decreased in activity. Furthermore, the study showed that combination of Fractions A, B, and C with osteoporotic drug (pamidronate) further increased the activity and upregulated the gene expression of BMP-2 and Runx2. Conclusions. These findings demonstrated that polyphenols from semipurified fractions of QI enhanced bone formation through expression of the investigated bone-related marker that is its potential role when combined with readily available osteoporotic drug.

Combinatorial Therapeutic Discovery Using Perturbational Gene-Expression Signatures

2018 ◽  
Author(s):  
Chen-Tsung Huang ◽  
Chiao-Hui Hsieh ◽  
Yun-Hsien Chung ◽  
Yen-Jen Oyang ◽  
Hsuan-Cheng Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Signatures

scholarly journals The Integrated RNA Landscape of Renal Preconditioning against Ischemia-Reperfusion Injury

2020 ◽  
Vol 31 (4) ◽  
pp. 716-730 ◽  
Author(s):  
Marc Johnsen ◽  
Torsten Kubacki ◽  
Assa Yeroslaviz ◽  
Martin Richard Späth ◽  
Jannis Mörsdorf ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reperfusion Injury ◽  
Caloric Restriction ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Hypoxic Preconditioning ◽  
Preventive Strategies ◽  
Gene Expression Signatures

BackgroundAlthough AKI lacks effective therapeutic approaches, preventive strategies using preconditioning protocols, including caloric restriction and hypoxic preconditioning, have been shown to prevent injury in animal models. A better understanding of the molecular mechanisms that underlie the enhanced resistance to AKI conferred by such approaches is needed to facilitate clinical use. We hypothesized that these preconditioning strategies use similar pathways to augment cellular stress resistance.MethodsTo identify genes and pathways shared by caloric restriction and hypoxic preconditioning, we used RNA-sequencing transcriptome profiling to compare the transcriptional response with both modes of preconditioning in mice before and after renal ischemia-reperfusion injury.ResultsThe gene expression signatures induced by both preconditioning strategies involve distinct common genes and pathways that overlap significantly with the transcriptional changes observed after ischemia-reperfusion injury. These changes primarily affect oxidation-reduction processes and have a major effect on mitochondrial processes. We found that 16 of the genes differentially regulated by both modes of preconditioning were strongly correlated with clinical outcome; most of these genes had not previously been directly linked to AKI.ConclusionsThis comparative analysis of the gene expression signatures in preconditioning strategies shows overlapping patterns in caloric restriction and hypoxic preconditioning, pointing toward common molecular mechanisms. Our analysis identified a limited set of target genes not previously known to be associated with AKI; further study of their potential to provide the basis for novel preventive strategies is warranted. To allow for optimal interactive usability of the data by the kidney research community, we provide an online interface for user-defined interrogation of the gene expression datasets (http://shiny.cecad.uni-koeln.de:3838/IRaP/).

scholarly journals Candidate therapeutic agents for hepatocellular cancer can be identified from phenotype-associated gene expression signatures

Cancer ◽  
2009 ◽  
Vol 115 (16) ◽  
pp. 3738-3748 ◽  
Author(s):  
Chiara Braconi ◽  
Fanyin Meng ◽  
Erica Swenson ◽  
Lyudmyla Khrapenko ◽  
Nianyuan Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatocellular Cancer ◽  
Therapeutic Agents ◽  
Gene Expression Signatures
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