scholarly journals Darkfield Imaging of Platelet Cytoskeleton-Granule Interactions

2014 ◽  
Vol 106 (2) ◽  
pp. 525a
Author(s):  
Solaire Finkenstaedt-Quinn ◽  
Christy L. Haynes
Keyword(s):  
Author(s):  
Carl Nail

Abstract To overcome the obstacles in preparing high-precision cross-sections of 'blind' bond wires in integrated circuits, this article proposes a different technique that generates reliable, repeatable cross-sections of bond wires across most or all of their lengths, allowing unencumbered and relatively artifact-free analysis of a given bond wire. The basic method for cross-sectioning a 'blind' bond wire involves radiographic analysis of the sample and metallographic preparation of the sample to the plane of interest. This is followed by tracking the exact location of the plane on the original radiograph using a stereomicroscope and finally darkfield imaging in which the wire is clearly visible with good resolution.


1996 ◽  
Vol 429 ◽  
Author(s):  
Patricia A. O'Neil ◽  
Katherine E. Violette ◽  
Mehmet C. Öztürk ◽  
Dale Batchelor ◽  
Dennis M. Maher

AbstractIn this paper, we explore selective Si epitaxy by UltraHigh Vacuum Rapid Thermal Chemical Vapor Deposition (UHV-RTCVD) using Si2H6, H2, and C12 with particular emphasis on selectivity robustness. Two key parameters considered in this study were partial pressures of Si2H6 and H2. It was found that excessive increases in either partial pressure could lead to selectivity degradation. The two mechanisms by which the observed selectivity degradation can be explained are as follows: A higher Si2H6 partial pressure provides a larger flux of Si atoms which directly influences the probability of reaching the critical nuclei size for stable nuclei formation while an increase in H2 partial pressure reduces the desorption rate of Si adatoms from the insulator surface by reducing the available Cl in the gas phase for SiC12 formation. The impact of process parameters on epitaxial defect density was also evaluated using darkfield imaging. The results clearly indicate increasing defect density upon increases in both the chlorine flow rate and the level of contamination introduced through the silicon source gas.


2021 ◽  
Vol 63 (7) ◽  
pp. 623-629
Author(s):  
André Hilger ◽  
Nikolay Kardjilov ◽  
Axel Lange ◽  
Andreas Kupsch ◽  
Manfred P. Hentschel ◽  
...  

Abstract While X-ray based darkfield imaging with grating interferometers is already widely used, darkfield imaging with neutrons has still a relatively small user community focused mostly on magnetic materials. Here, we demonstrate the application of neutron darkfield imaging byTalbot-Lau type grating interferometry to fiber reinforced plastics. Common carbon and glass fiber composites have been investigated including characteristic damage structures. The darkfield images show a strong signal response caused by fiber delamination, suitable fiber direction, particles, pores and cracks. The basic principles of neutron darkfield imaging applied to fiber composites are highlighted.


Author(s):  
Masahiko Sato ◽  
Janice Herring ◽  
John Kim ◽  
Eli Lilly ◽  

Reflected polarized light microscopy (Fig. 1A) was used previously to generate high contrast images of birefringent and light scattering samples, including bone surfaces and autoradiographic specimens. We now present a modification (Fig. 1B) of the Gullberg system with improved sensitivity for the characterization of bone specimens and quantitation of silver granules on autoradiographic specimens. Reflected imaging techniques were useful to generate high contrast images superior to transmitted light strategies, and both of the strategies presented can be adapted easily to any fluorescence microscope.Reflected light produced images free of refractile noise from materials through the thickness of the specimen which detracts from transmission darkfield imaging of silver grains and brightfield imaging of bone surfaces. The use of crossed polars also eliminated noise from stray light reflected off of internal microscope elements. The rotatable lambda/4 plate mounted on the objective front element (Fig. 1A) allowed considerable manipulation of image contrast, permitting dual imaging of silver granules, birefringent tissues in autoradiographic specimens and the surface topography of bone specimens by rotating the lambda/4 plate to 45°.


2013 ◽  
Author(s):  
Natasha Fairbairn ◽  
Rute Fernandes ◽  
Rachel Carter ◽  
Timothy J. Elliot ◽  
Antonios G. Kanaras ◽  
...  

2014 ◽  
Vol 6 (23) ◽  
pp. 9202-9205 ◽  
Author(s):  
Hui Shen ◽  
Bin Xiong ◽  
Ruili Xu ◽  
Xiaodong Cheng ◽  
Yan He ◽  
...  

2013 ◽  
Vol 23 (3) ◽  
pp. 228-235 ◽  
Author(s):  
Gisela Anton ◽  
Florian Bayer ◽  
Matthias W. Beckmann ◽  
Jürgen Durst ◽  
Peter A. Fasching ◽  
...  

2017 ◽  
Author(s):  
A. Orth ◽  
E. R. Wilson ◽  
J. G. Thompson ◽  
B. C. Gibson

ABSTRACTMobile phone microscopes are a natural platform for point-of-care imaging, but current solutions require an externally powered illumination source, thereby adding bulk and cost. We present a mobile phone microscope that uses the internal flash or sunlight as the illumination source, thereby reducing complexity whilst maintaining functionality and performance. The microscope is capable of both brightfield and darkfield imaging modes, enabling microscopic visualization of samples ranging from plant to mammalian cells. We describe the microscope design principles, assembly process, and demonstrate its imaging capabilities through the visualization of unlabelled cell nuclei to observing the motility of cattle sperm.


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