scholarly journals The Effect of Proteins and Lipids on Membrane Stiffness

2016 ◽  
Vol 110 (3) ◽  
pp. 243a
Author(s):  
Philip W. Fowler ◽  
Anna Duncan ◽  
Jean Helie ◽  
Matthieu Chavent ◽  
Heidi Koldsø ◽  
...  
Keyword(s):  
Author(s):  
Dean A. Handley ◽  
Lanping A. Sung ◽  
Shu Chien

RBC agglutination by lectins represents an interactive balance between the attractive (bridging) force due to lectin binding on cell surfaces and disaggregating forces, such as membrane stiffness and electrostatic charge repulsion (1). During agglutination, critical geometric parameters of cell contour and intercellular distance reflect the magnitude of these interactive forces and the size of the bridging macromolecule (2). Valid ultrastructural measurements of these geometric parameters from agglutinated RBC's require preservation with minimal cell distortion. As chemical fixation may adversely influence RBC geometric properties (3), we used chemical fixation and cryofixation (rapid freezing followed by freeze-substitution) as a comparative approach to examine these parameters from RBC agglutinated with Ulex I lectin.


Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 727-734 ◽  
Author(s):  
K Kon ◽  
N Maeda ◽  
T Shiga

The effect of shear force (depending on shear rate and viscosity of extracellular medium) and hematocrit of RBC suspension on RBC deformation was studied quantitatively using a cone-plate rheoscope with various kinds of cells, ie, partially hemolyzed (PH) cells, density-fractionated intact cells, and diamide-treated cells. The deformation index (DI) of ellipsoidally deformed cells was shown to be a function of beta gamma eta ex(eta ex/eta in)alpha, where gamma eta ex is applied shear stress, eta ex and eta in are external and internal viscosities, respectively, and alpha and beta are adjustable parameters related to the membrane viscoelastic properties. The increase of suspension viscosity at higher hematocrits (Hts) generally enhanced the ellipsoidal deformation of cells, in the same manner as increasing the suspending medium viscosity of a diluted cell suspension. The suppressing effect on cell deformation appeared above a certain Ht. When intact cells were mixed with glutaraldehyde-treated, hardened cells, the ellipsoidal deformation of intact cells was disturbed. The suppression of deformation probably occurred through disturbance of laminar flow-lines around intact cells.


2018 ◽  
Author(s):  
Dong-Yuan Chen ◽  
Justin Crest ◽  
Sebastian J. Streichan ◽  
David Bilder

ABSTRACTOrgans are sculpted by extracellular as well as cell-intrinsic forces, but how collective cell dynamics are orchestrated in response to microenvironmental cues is poorly understood. Here we apply advanced image analysis to reveal ECM-responsive cell behaviors that drive elongation of the Drosophila follicle, a model 3D system in which basement membrane stiffness instructs tissue morphogenesis. Through in toto morphometric analyses of WT and ‘round egg’ mutants, we find that neither changes in average cell shape nor oriented cell division are required for appropriate organ shape. Instead, a major element is a reorientation of elongated cells at the follicle anterior. Polarized reorientation is regulated by mechanical cues from the basement membrane, which are transduced by the Src tyrosine kinase to alter junctional E-cadherin trafficking. This mechanosensitive cellular behavior represents a conserved mechanism that can elongate ‘edgeless’ tubular epithelia in a process distinct from those that elongate bounded, planar epithelia.


2008 ◽  
Vol 94 (7) ◽  
pp. 2529-2545 ◽  
Author(s):  
Qiang Zhu ◽  
Robert J. Asaro

2018 ◽  
Vol 24 (9) ◽  
pp. 2754-2766 ◽  
Author(s):  
Xiaobao Li ◽  
Changwen Mi

Previous studies demonstrate that, for nanostructures under transverse bending, the effective Young modulus is appreciably greater (in magnitude) than that of the same elements under axial loads. Therefore, in addition to the conventional residual surface tension and membrane stiffness, the curvature dependence of surface energy is desired for inhomogeneously strained nanostructures. In this paper, we aim to reevaluate the size-dependent nanoindentation hardness of an elastic half-space subjected to nanosized frictionless traction, through the use of both the curvature-independent Gurtin–Murdoch and the curvature-dependent Steigmann–Ogden models of surface elasticity. The nanoindentation problem is solved by the integration of Boussinesq’s method of displacement potentials and Hankel integral transforms. As examples, the effects of residual surface tension, membrane stiffness, and bending rigidity of the half-space boundary are parametrically analyzed in detail for a uniform circular pressure and a concentrated normal force. The observations in semianalytical calculations suggest a significant difference in the nanoindentation hardnesses predicted from the two popular models of surface mechanics. In most cases, the inclusion of bending rigidity results in smaller displacements and stresses, and therefore higher indentation hardness. Based on physically interpretable numerical values of surface material properties, we show that a curvature-dependent model of surface elasticity is required in order to characterize the size-dependent feature of nanoindentation problems correctly.


Hypertension ◽  
2001 ◽  
Vol 38 (5) ◽  
pp. 1158-1161 ◽  
Author(s):  
Hayden Huang ◽  
Roger D. Kamm ◽  
Peter T.C. So ◽  
Richard T. Lee

2015 ◽  
Author(s):  
Shirin Farrahi ◽  
Jonathan B. Sellon ◽  
Roozbeh Ghaffari ◽  
Dennis M. Freeman

2013 ◽  
Vol 10 (86) ◽  
pp. 20130403 ◽  
Author(s):  
David Schley ◽  
Robert J. Whittaker ◽  
Benjamin W. Neuman

Viral replication occurs within cells, with release (and onward infection) primarily achieved through two alternative mechanisms: lysis, in which virions emerge as the infected cell dies and bursts open; or budding, in which virions emerge gradually from a still living cell by appropriating a small part of the cell membrane. Virus budding is a poorly understood process that challenges current models of vesicle formation. Here, a plausible mechanism for arenavirus budding is presented, building on recent evidence that viral proteins embed in the inner lipid layer of the cell membrane. Experimental results confirm that viral protein is associated with increased membrane curvature, whereas a mathematical model is used to show that localized increases in curvature alone are sufficient to generate viral buds. The magnitude of the protein-induced curvature is calculated from the size of the amphipathic region hypothetically removed from the inner membrane as a result of translation, with a change in membrane stiffness estimated from observed differences in virion deformation as a result of protein depletion. Numerical results are based on experimental data and estimates for three arenaviruses, but the mechanisms described are more broadly applicable. The hypothesized mechanism is shown to be sufficient to generate spontaneous budding that matches well both qualitatively and quantitatively with experimental observations.


Author(s):  
Sarah Becker ◽  
Katrin Schulz ◽  
Dennis Scherhaufer ◽  
Peter Gumbsch ◽  
Christian Greiner

Abstract The microstructure of the materials constituting a metallic frictional contact strongly influences tribological performance. Being able to tailor friction and wear is challenging due to the complex microstructure evolution associated with tribological loading. Here, we investigate the effect of the strain distribution on these processes. High-purity copper plates were morphologically surface textured with two parallel rectangles—referred to as membranes—over the entire sample length by micro-milling. By keeping the width of these membranes constant and only varying their height, reciprocating tribological loading against sapphire discs resulted in different elastic and plastic strains. Finite element simulations were carried out to evaluate the strain distribution in the membranes. It was found that the maximum elastic strain increases with decreasing membrane stiffness. The coefficient of friction decreases with increasing membrane aspect ratio. By analyzing the microstructure and local crystallographic orientation, we found that both show less change with decreasing membrane stiffness. Graphic abstract


Sign in / Sign up

Export Citation Format

Share Document