FTIR Study of the Biochemical Effects Induced by X-Ray Irradiations Combined with GD Nanoparticles in F98 Glioma Cells

Background: Radiotherapy is used as one of the most effective regimens for cancer treatment, while radioresistance is a major drawback in cancer treatment. Objectives: The aim of this study was to evaluate the sensitizing effect of olanzapine (OLA) with X-ray on glioblastoma (U-87 MG) cells death. Methods: The synergistic killing effect of OLA with ionizing radiation (IR) on glioma was evaluated by colony formation assay. The generations of reactive oxygen species (ROS) and protein carbonyl (PC) as oxidized protein were determined in OLA and irradiated cells. Results: The results of this study showed that OLA reduced the number of colonies in irradiated glioma cells. OLA elevated ROS and PC levels in irradiated cells. The synergistic killing effect of OLA with IR in U-87 MG cell was observed at concentrations 1 µM and 20 µM of OLA. The maximum radiosensitizing effect of OLA was observed at concentration of 20 µM. Conclusion: The present study demonstrates that OLA has radiosensitizing effect on cell death induced by IR in glioma cells.

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Superparamagnetic iron oxide nanoparticles enhance glioma radiosensitivity via inducing cell cycle arrest and apoptosis

10.21203/rs.3.rs-93279/v1 ◽

2020 ◽

Author(s):

Jinning Mao ◽

Meng Jiang ◽

Xingliang Dai ◽

Guodong Liu ◽

Zhixiang Zhuang ◽

...

Keyword(s):

Cell Cycle ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Glioma Cells ◽

Superparamagnetic Iron Oxide ◽

Spherical Shape ◽

Treated Group ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

X Ray

Abstract Aim: Superparamagnetic iron oxide nanoparticles (SPIONs) is a widely used biomedical material for imaging and targeting drug delivery. We synthesized SPIONs and tested their effects on the radiosensitization of glioma.Methods: Acetylated 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (Fe3O4 NPs) were synthesized via a one-step hydrothermal approach and the surface was chemically modified with acetic anhydride to generate surface charge-neutralized NPs. NPs were characterized by TEM and ICP-AES. Radiosensitivity of U87MG glioma cells was evaluated by MTT assay. Cell cycle and apoptosis in glioma cells were examined by flow cytometry. Results: APTS-coated Fe3O4 NPs had a spherical or quasi-spherical shape with average size of 10.5±1.1 nm. NPs had excellent biocompatibility and intracellular uptake of NPs reached the peak 24 hours after treatment. U87 cell viability decreased significantly after treatment with both X-ray and NPs compared to X-ray treatment alone. Compared to X-ray treatment alone, the percentage of cells in G2/M phase (31.83%) significantly increased in APTS-coated Fe3O4 NPs plus X-ray treated group (P<0.05). In addition, the percentage of apoptotic cells was significant higher in APTS-coated Fe3O4 NPs plus X-ray treated group than in X-ray treatment alone group (P<0.05). Conclusion: APTS-coated Fe3O4 NPs achieved excellent biocompatibility and increased radiosensitivity for glioma cells.

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Lethal Effect of X-ray and ACNU on Cultured Rat Glioma Cells in Multicellular Spheroids

Neurologia medico-chirurgica ◽

10.2176/nmc.24.758 ◽

1984 ◽

Vol 24 (10) ◽

pp. 758-766 ◽

Cited By ~ 4

Author(s):

Satoru SUGIYAMA ◽

Teruaki MORI ◽

Jiro SUZUKI ◽

Takehito SASAKI

Keyword(s):

Glioma Cells ◽

Lethal Effect ◽

Multicellular Spheroids ◽

X Ray ◽

Rat Glioma

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Infrared study of the biochemical effects in glioma cells induced by x-rays and Gd nanoparticles: first studies at SESAME synchrotron (Jordan)

Radiotherapy and Oncology ◽

10.1016/s0167-8140(16)30145-1 ◽

2016 ◽

Vol 118 ◽

pp. S71

Author(s):

I. Yousef ◽

O. Seksek ◽

J. Sulé-Suso ◽

S. Gil ◽

Y. Prezado ◽

...

Keyword(s):

Glioma Cells ◽

X Rays ◽

Infrared Study ◽

Biochemical Effects

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Multiple biochemical effects of a series of X-ray induced mutations at the albino locus in the mouse

Biochemical Genetics ◽

10.1007/bf00485588 ◽

1973 ◽

Vol 9 (1) ◽

pp. 25-39 ◽

Cited By ~ 28

Author(s):

Janet Thorndike ◽

Monica J. Trigg ◽

Richard Stockert ◽

Salome Gluecksohn-Waelsch ◽

Carl F. Cori

Keyword(s):

Induced Mutations ◽

X Ray ◽

Biochemical Effects

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Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression

Scientific Reports ◽

10.1038/s41598-021-01475-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yuichiro Tsuji ◽

Naosuke Nonoguchi ◽

Daisuke Okuzaki ◽

Yusuke Wada ◽

Daisuke Motooka ◽

...

Keyword(s):

Survival Time ◽

Brain Tissue ◽

Median Survival ◽

Median Survival Time ◽

Glioma Cell ◽

Right Hemisphere ◽

Glioma Cells ◽

Chronic Phase ◽

Normal Brain ◽

X Ray

AbstractRadiation therapy is one of standard treatment for malignant glioma after surgery. The microenvironment after irradiation is considered not to be suitable for the survival of tumor cells (tumor bed effect). This study investigated whether the effect of changes in the microenvironment of parenchymal brain tissue caused by radiotherapy affect the recurrence and progression of glioma. 65-Gy irradiation had been applied to the right hemisphere of Fisher rats. After 3 months from irradiation, we extracted RNA and protein from the irradiated rat brain. To study effects of proteins extracted from the brains, we performed WST-8 assay and tube formation assay in vitro. Cytokine production were investigated for qPCR. Additionally, we transplanted glioma cell into the irradiated and sham animals and the median survival time of F98 transplanted rats was also examined in vivo. Immunohistochemical analyses and invasiveness of implanted tumor were evaluated. X-ray irradiation promoted the secretion of cytokines such as CXCL12, VEGF-A, TGF-β1 and TNFα from the irradiated brain. Proteins extracted from the irradiated brain promoted the proliferation and angiogenic activity of F98 glioma cells. Glioma cells implanted in the irradiated brains showed significantly high proliferation, angiogenesis and invasive ability, and the post-irradiation F98 tumor-implanted rats showed a shorter median survival time compared to the Sham-irradiation group. The current study suggests that the microenvironment around the brain tissue in the chronic phase after exposure to X-ray radiation becomes suitable for glioma cell growth and invasion.

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