Surface Charge Density Measurement of a Single Protein Molecule with a Controlled Orientation by AFM

Author(s):  
Yuki Yamamoto ◽  
Hiroaki Kominami ◽  
Kei Kobayashi ◽  
Hirofumi Yamada
2021 ◽  
Author(s):  
Amit Kumar ◽  
V. Subramanian ◽  
Shailesh Joshi ◽  
B. Venkatraman

In the present ongoing pandemic, the N95 respirator is an essential protective barrier to suppress the spread of the SARS-Cov-2 virus and protect the frontline worker from exposure. The N95 respirators are meant for single usage; however, they can be used after decontamination in-light of the economy and shortfall in availability. At this juncture, the respirators performance after various types of sterilization and usage condition is required to be analyzed in detail. With this motto, this work has proceeded. The filtration efficiency, pressure drop, and quality factor of the respirator are evaluated for two face velocities (5.8 and 26.4 cm/s) following different sterilization methods. Sterilization techniques used here are dry air oven heating, gamma irradiation, and immersing in a 10% concentration of liquid hydrogen peroxide. The particle filtration performance and electrostatic surface charge density measurement are used to determine the facemasks efficacy after sterilization. The methods recommended to sterilize N95 masks without affecting their performance are (i) using dry air heat at 80oC and (ii) H2O2 soaking. The highest reduction in filtration efficiency is observed to be 30-35% after gamma irradiation due to a change in the electrostatic properties of the respirator layers. However, the filtration efficiency does not change significantly for other sterilization methods despite a change in charge density, but there is no direct correlation with filtration efficiency. Electrostatic charge measurement of the filtration layer is a crucial indicator of filtration efficiency degradation. Policymakers can use these data during potential future N95 shortage to assess the viability of sterilization methods.


2003 ◽  
Vol 58 (1-2) ◽  
pp. 45-58 ◽  
Author(s):  
Akiko Kumada ◽  
Yasuhiro Shimizu ◽  
Masakuni Chiba ◽  
Kunihiko Hidaka

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.


Author(s):  
Linards Lapčinskis ◽  
Artis Linarts ◽  
Kaspars Mālnieks ◽  
Hyunseung Kim ◽  
Kristaps Rubenis ◽  
...  

In this study, we investigate triboelectrification in polymer-based nanocomposites using identical polymer matrixes containing different concentrations of nanoparticles (NPs). The triboelectric surface charge density on polymer layers increased as the...


2021 ◽  
Vol 22 (5) ◽  
pp. 2270
Author(s):  
Joanna Kotyńska ◽  
Monika Naumowicz

Interactions between phospholipid membranes and selected drugs affecting the central nervous system (CNS) were investigated. Small, unilamellar liposomes were used as biomimetic cell membrane models. Microelectrophoretic experiments on two-component liposomes were performed using the electrophoretic light scattering technique (ELS). The effect of both positively (perphenazine, PF) and negatively (barbituric acid, BA) charged drugs on zwitterionic L-α-phosphatidylcholine (PC) membranes were analyzed. Experimental membrane surface charge density (d) data were determined as a function of pH. Quantitative descriptions of the adsorption equilibria formed due to the binding of solution ions to analyzed two-component membranes are presented. Binding constants of the solution ions with perphenazine and barbituric acid-modified membranes were determined. The results of our research show that both charged drugs change surface charge density values of phosphatidylcholine membranes. It can be concluded that perphenazine and barbituric acid are located near the membrane surface, interacting electrostatically with phosphatidylcholine polar heads.


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