Effect of molecular weight of condensed tannins from warm-season perennial legumes on ruminal methane production in vitro

2013 ◽  
Vol 50 ◽  
pp. 154-162 ◽  
Author(s):  
Harley D. Naumann ◽  
Luis O. Tedeschi ◽  
James P. Muir ◽  
Barry D. Lambert ◽  
Merwyn M. Kothmann
Keyword(s):  
Molecular Weight ◽  
Methane Production ◽  
Condensed Tannins ◽  
Warm Season ◽  
Perennial Legumes

scholarly journals Relationships between Structures of Condensed Tannins from Texas Legumes and Methane Production During In Vitro Rumen Digestion

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2123 ◽  
Author(s):  
Harley Naumann ◽  
Rebecka Sepela ◽  
Aira Rezaire ◽  
Sonia Masih ◽  
Wayne Zeller ◽  
...  
Keyword(s):  
Methane Production ◽  
Condensed Tannins ◽  
Warm Season ◽  
2D Nmr ◽  
Plant Sample ◽  
Ruminal Ph ◽  
Ch4 Production ◽  
Acacia Angustissima ◽  
Perennial Legumes

Previous studies showed that a series of purified condensed tannins (CTs) from warm-season perennial legumes exhibited high variability in their modulation of methane production during in vitro rumen digestion. The molecular weight differences between these CTs did not provide correlation with either the in vitro CH4 production or the ability to precipitate bovine serum albumin. In an effort to delineate other structure-activity relationships from these methane abatement experiments, the structures of purified CTs from these legumes were assessed with a combination of methanolysis, quantitative thiolysis, 1H-13C HSQC NMR spectroscopy and ultrahigh-resolution MALDI-TOF MS. The composition of these CTs is very diverse: procyanidin/prodelphinidin (PC/PD) ratios ranged from 98/2 to 2/98; cis/trans ratios ranged from 98/2 to 34/66; mean degrees of polymerization ranged from 6 to 39; and % galloylation ranged from 0 to 75%. No strong correlation was observed between methane production and the protein precipitation capabilities of the CT towards three different proteins (BSA, lysozyme, and alfalfa leaf protein) at ruminal pH. However, a strong non-linear correlation was observed for the inhibition of methane production versus the antioxidant activity in plant sample containing typical PC- and PD-type CTs. The modulation of methane production could not be correlated to the CT structure (PC/PD or cis/trans ratios and extent of galloylation). The most active plant in methane abatement was Acacia angustissima, which contained CT, presenting an unusual challenge as it was resistant to standard thiolytic degradation conditions and exhibited an atypical set of cross-peak signals in the 2D NMR. The MALDI analysis supported a 5-deoxy flavan-3-ol-based structure for the CT from this plant.

scholarly journals Molecular weight and protein-precipitating ability of condensed tannins from warm-season perennial legumes

2013 ◽  
Vol 9 (1) ◽  
pp. 212-219 ◽  
Author(s):  
H.D. Naumann ◽  
A.E. Hagerman ◽  
B.D. Lambert ◽  
J.P. Muir ◽  
L.O. Tedeschi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Condensed Tannins ◽  
Warm Season ◽  
Perennial Legumes

scholarly journals PSXI-35 Effects of chitosan source, molecular weight and supplementation level on in vitro (RUSITEC) ammonia and methane production.

2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 424-425
Author(s):  
S Kirwan ◽  
T Boland ◽  
A Kelly ◽  
E Serra ◽  
G Rajauria ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Methane Production

In vitro methane production and tolerance to condensed tannins in five ruminant species

2015 ◽  
Vol 205 ◽  
pp. 1-9 ◽  
Author(s):  
Ives C.S. Bueno ◽  
Roberta A. Brandi ◽  
Raul Franzolin ◽  
Gabriela Benetel ◽  
Gisele M. Fagundes ◽  
...  
Keyword(s):  
Methane Production ◽  
Condensed Tannins ◽  
Ruminant Species

scholarly journals PSXI-26 Effects of condensed tannins in purple prairie and white prairie clover on ruminal fermentation and methane production in vitro.

2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 422-422 ◽  
Author(s):  
Q Huang ◽  
K Peng ◽  
A Iwaasa ◽  
M Schellenberg ◽  
T McAllister ◽  
...  
Keyword(s):  
Methane Production ◽  
Condensed Tannins ◽  
Ruminal Fermentation

scholarly journals Methane production from in vitro incubation of kikuyu grass, lucerne and forages containing condensed tannins

2005 ◽  
pp. 147-153 ◽  
Author(s):  
M.H. Tavendale ◽  
L.P. Meagher ◽  
Z.A. Park-Ng ◽  
G.C. Waghorn ◽  
G.T. Attwood
Keyword(s):  
Fatty Acid ◽  
New Zealand ◽  
Methane Production ◽  
Condensed Tannins ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Methane Emissions ◽  
Kikuyu Grass

A series of in vitro incubations with kikuyu grass (Pennisetum clandestinum), lucerne and six legumes containing condensed tannins (CT) were undertaken to evaluate this technique against in vivo trials in New Zealand, measuring methane emissions. Published trials have demonstrated a reduction in methane emissions associated with CT and in one instance from kikuyu. The incubations used fresh minced forage (equivalent to 0.5 g dry matter (DM)) and were carried out in 50 ml sealed bottles containing buffer and rumen inoculum. Gas was sampled through a septum to monitor volume and composition throughout the 24h incubation. Incubation for 24 h resulted in 2.4-6.6 % conversion of DM to methane, and suggested CT concentrations below about 8% of the DM can reduce methane production without inhibiting fermentation rate. Higher concentrations of CT (> 8%) were associated with a lower rate of digestion. In common with in vivo trials, CT concentration in forage DM was inversely related to methane (adjusted R2 = 0.49; P = 0.01) and volatile fatty acid (adjusted R2=0.86; P

Effect of molecular weight and concentration of legume condensed tannins on in vitro larval migration inhibition of Haemonchus contortus

2014 ◽  
Vol 199 (1-2) ◽  
pp. 93-98 ◽  
Author(s):  
H.D. Naumann ◽  
S.A. Armstrong ◽  
B.D. Lambert ◽  
J.P. Muir ◽  
L.O. Tedeschi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Haemonchus Contortus ◽  
Condensed Tannins ◽  
Migration Inhibition ◽  
Larval Migration

scholarly journals Impact of variation in structure of condensed tannins from sainfoin (Onobrychis viciifolia ) on in vitro ruminal methane production and fermentation characteristics

2015 ◽  
Vol 100 (2) ◽  
pp. 348-360 ◽  
Author(s):  
B. Hatew ◽  
E. Stringano ◽  
I. Mueller-Harvey ◽  
W. H. Hendriks ◽  
C. Hayot Carbonero ◽  
...  
Keyword(s):  
Methane Production ◽  
Condensed Tannins ◽  
Onobrychis Viciifolia

Molecular weight of condensed tannins of some tropical feed-leaves and their effect on in vitro gas and methane production

2019 ◽  
Vol 59 (12) ◽  
pp. 2154 ◽  
Author(s):  
A. Petlum ◽  
P. Paengkoum ◽  
J. B. Liang ◽  
K. Vasupen ◽  
S. Paengkoum
Keyword(s):  
Plant Species ◽  
Methane Production ◽  
Condensed Tannins ◽  
Gas Production ◽  
Published Data ◽  
Plant Materials ◽  
Molecular Weights ◽  
Tropical Plant ◽  
Neem Leaves

The concentration and molecular weights (MW) of condensed tannins (CT) of three locally available tropical plant species leaves which have potential to be used as ruminant feed, and their effects on in vitro gas, including methane, production were investigated. Leaves of three plant species, namely, leucaena (Leucaena leucocephala), cassava (Manihot esculenta, Cranzt), and Siamese neem (Azadirachta indica A.Juss. var. Siamensis Valeton) were used in the present study. CT contents ranged from 1.2% in the leucaena to 5.0% in Siamese neem. The weight-average molecular weights (Mw) of the purified CTs, determined using gel-permeation chromatography, were 3222, 3409 and 3612 Da for leucaena, cassava and Siamese neem respectively. The above values were within the range reported for CTs of various tropical plant species. We know of no published data on MW of CTs from leaves of cassava and Siamese neem and, thus, this research, for the first time, reported the MWs of the above two plant materials. Subsequently, two CT extracts with differing MWs, such as CTs extracted from leaves of Siamese neem and leaves of leucaena, were selected as the representative of high and low MWs of CT respectively, and used for investigation of the effect of the MW of CT on in vitro gas production and fermentation parameters. Supplementation of CTs of a higher MW extracted from leaves of Siamese neem (at 2–6 mg/100 mg DM) significantly inhibited in vitro total gas and methane production, while supplementation of CTs with a lower MW extracted from leaves of leucaena had no effect, except for total gas production at the highest level (6 mg/100 mg DM) of supplementation. Similarly, CT from Siamese neem leaves had a stronger inhibitory effect (P < 0.001) on in vitro volatile fatty acid, acetic acid and butyric acid production. The above results indicated that concentrations and the MW of CT varied among the plant species; in addition, the efficacy of CTs to inhibit ruminal CH4 emission are influenced by their MW.

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