scholarly journals A comparative study of Lonicera japonica with related species: Morphological characteristics, ITS sequences and active compounds

2014 ◽  
Vol 54 ◽  
pp. 198-207 ◽  
Author(s):  
Qingxia Wang ◽  
Qingmei Quan ◽  
Xueli Zhou ◽  
Yunguo Zhu ◽  
Yunlong Lan ◽  
...  
Phytotaxa ◽  
2015 ◽  
Vol 213 (1) ◽  
pp. 46 ◽  
Author(s):  
Hai-Sheng Yuan

Two new wood-decaying polypores from southern China, Grammothele separabillima and Theleporus rimosus, are described and illustrated using morphological characteristics and rDNA ITS sequences. Grammothele separabillima is characterized by its easily separable basidiocarps, poroid hymenophore with hymenium restricted to the base of the tubes, a dimitic hyphal system, pale brown skeletal hyphae with dextrinoid reaction, presence of dendrohyphidia, and oblong ellipsoid basidiospores. Theleporus rimosus is characterized by its resupinate, cracked basidiocarps, shallow poroid hymenophore with hymenium restricted to the base of the tubes, a di-trimitic hyphal system with dendroid and non-dextrinoid skeletal hyphae, presence of dendrohyphidia and ellipsoid to fusiform basidiospores. The discriminating characters of these two new species and the closely related species are discussed.


Phytotaxa ◽  
2020 ◽  
Vol 446 (4) ◽  
pp. 229-236
Author(s):  
MENG ZHOU ◽  
FANG WU ◽  
XUE-MEI TIAN

A new poroid fungus, Nigrofomes submelanoporus, from tropical China, is described and illustrated based on morphological characteristics and phylogenetic analysis. The new species is characterized by perennial, resupinate to effused-reflexed, woody-hard and dark gray to almost black basidiocarps, lacerate dissepiments, a monomitic hyphal system, the presence of cystidioles and hyphal pegs, and broadly ellipsoid basidiospores (4–5.1 × 3.1–4.3 µm). Molecular analysis based on ITS sequences demonstrates the phylogenetic position of the new species in Nigrofomes. The discriminating characteristics between the new species and closely related species are discussed.


Sommerfeltia ◽  
2008 ◽  
Vol 31 (1) ◽  
pp. 161-177 ◽  
Author(s):  
U. Peintner

Cortinarius alpinus as an example for morphological and phylogenetic species concepts in ectomycorrhizal fungiExtensive morphological and molecular analyses of closely related species from alpine, subalpine and montane habitats should enable a comparison of ecological, morphological and phylogenetic species concepts in ectomycorrhizal mushrooms. One fundamental question of this study was whether alpine species really exist, and which criteria, besides the specific habitat, could reliably be used for the de-limitation of such taxa. For this reason, 56 rDNA ITS sequences were generated or downloaded from GenBank for 10 closely related species of Cortinarius subgenus Myxacium, section Myxacium. Several collections were sequenced for each of the following taxa: Cortinarius absarokensis, C. alpinus, C. favrei, C. fennoscandicus, C. grallipes, C. mucosus, C. muscigenus, C. septentrionalis, C. trivialis and C. vernicosus. Moreover, spore statistics were carried out for 38 collections of alpine and subalpine taxa. These data provide clear evidence for C. favrei being a synonym of C. alpinus. C. absarokensis and C. alpinus can clearly be delimited based on pileus diameter and average dry weight per basidiome, even in overlapping habitats, but spore size and shape is not a good distinguishing character. Phylograms have very short branches, and base differences between ITS sequences are generally very low in this group, and give no resolution for the included taxa of this section. Based on these results, species concepts of ectomycorrhizal mushrooms are discussed in detail.


2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Congzhao Fan ◽  
Xiaojin Li ◽  
Jun Zhu ◽  
Jingyuan Song ◽  
Hui Yao

The medicinal plantFerulahas been widely used in Asian medicine, especially in Uyghur medicine in Xinjiang, China. Given that various substitutes and closely related species have similar morphological characteristics,Ferulais difficult to distinguish based on morphology alone, thereby causing confusion and threatening the safe use ofFerula. In this study, internal transcribed spacer 2 (ITS2) sequences were analyzed and assessed for the accurate identification of two salableFerulaspecies (Ferula sinkiangensisandFerula fukangensis) and eight substitutes or closely related species. Results showed that the sequence length of ITS2 ranged from 451 bp to 45 bp, whereas guanine and cytosine contents (GC) were from 53.6% to 56.2%. A total of 77 variation sites were detected, including 63 base mutations and 14 insertion/deletion mutations. The ITS2 sequence correctly identified 100% of the samples at the species level using the basic local alignment search tool 1 and nearest-distance method. Furthermore, neighbor-joining tree successfully identified the genuine plantsF. sinkiangensisandF. fukangensisfrom their succedaneum and closely related species. These results indicated that ITS2 sequence could be used as a valuable barcode to distinguish Uyghur medicineFerulafrom counterfeits and closely related species. This study may broaden DNA barcoding application in the Uyghur medicinal plant field.


2017 ◽  
Author(s):  
Qiuju Yang ◽  
Ze-Jun Hu

Abstract. Aurora is a very important geophysical phenomenon in the high latitude of Arctic and Antarctic regions, and it is significant to make a comparative study of the auroral morphology between the two hemispheres. Based on the morphological characteristics of the four labeled dayside auroral types (include auroral arc, drapery corona, radial corona and hot-spot aurora) on the 8001 dayside auroral images at Chinese Yellow River Station in 2003, and by extracting the local binary pattern (LBP) features and using k-nearest classifier, this paper makes an automatic classification to the 65361 auroral images of the Chinese Yellow River Station during 2004–2009 and the 39335 auroral images of the South Pole Station between 2003–2005, and finally obtains the occurrence distribution of the dayside auroral morphology in northern and southern hemispheres. The statistical results indicate that the four auroral types present similar occurrence distribution between the two stations. To the best of our knowledge, we are the first to report the statistical comparative results of dayside auroral morphology distribution between northern and southern hemispheres.


1990 ◽  
Vol 105 (2) ◽  
pp. 245-254 ◽  
Author(s):  
E. A. Szabo ◽  
P. M. Desmarchelier

SUMMARYNinety-six isolates of presumptive or confirmedListeria monocytogeneswere obtained from local clinical (30 isolates) or food laboratories (66 isolates). Minimal biochemical analysis identified only 80% of these isolates asL. monocytogenesthe remaining includedL. seeligeri, 1%, or the non-haemolyticL. innocua, 19%. The 27 clinical and 50 food isolates, mainly from meat products, frozen confectionaries, and cheeses, confirmed asL. monocytogeneswere compared biochemically and serologically. Twenty-one isolates, including some strains ofL. innocuaandL. seeligeri, were examined for pathogenicity in immunocompromized mice and 44 typed using bacterial restriction endonuclease DNA analysis (BRENDA). Only isolates ofL. monocytogeneswere found to be pathogenic. Biovar-typing of the isolates was unreliable and provided poor discrimination. Serogroups 1/2 and 4 predominated among clinical and food isolates and BRENDA provided better discrimination among isolates. Ten stable and reproducible restriction patterns were observed among theListeriasp. isolates studied. Overall, a combination of techniques gave the best discrimination and indicated their potential for use as epidemiological tools.


2020 ◽  
Vol 106 (6) ◽  
pp. 1083-1087
Author(s):  
Alvaro Minuesa-Asensio ◽  
Francisco García-Esteo ◽  
José Ramón Mérida-Velasco ◽  
Carmen Barrio-Asensio ◽  
Crótida de la Cuadra-Blanco ◽  
...  

Diversity ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 123 ◽  
Author(s):  
Susannah Johnson-Fulton ◽  
Linda Watson

Species of the Cochlospermaceae, a small mostly pantropical plant family, were evaluated at a continental scale for medicinal uses in traditional medicine. This ethnobotanical information was placed in a phylogenetic framework to make informed predictions in the search for new medicines and bioactive compounds. Medicinal plant-use data were mapped onto a molecular phylogeny based on DNA sequences of nuclear and chloroplast markers. Associations of medicinal uses among closely related species occurring in different geographic regions and among diverse cultures were evaluated. The most common medicinal uses for these species are those used to treat skin ailments, gastro-intestinal problems, malaria, and liver issues. The plant species with the most numerous uses is Cochlospermum tinctorium, which occurs primarily in West Africa. Closely related species being used by cultural groups in different geographic regions to treat the same illnesses suggests the presence of bioactive compounds with potential biomedical value, since they may represent independent discoveries of similar medicinally-active compounds. This leads to the speculation that those closely related species not currently being used to treat these ailments may also contain identical or similar medicinally-active compounds and are worthy of laboratory investigations.


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