Metabolomics as a tool to discriminate species of the Ananas genus and assist in taxonomic identification

2022 ◽  
Vol 100 ◽  
pp. 104380
Author(s):  
Alvaro Luis Lamas Cassago ◽  
Fernanda Vidigal Duarte Souza ◽  
Guilherme Julião Zocolo ◽  
Fernando Batista da Costa
Keyword(s):  
Taxonomic Identification

scholarly journals Application of RAPD DNA fingerprinting in taxonomic identification of amphipods: a case-study with Gammarus species (Crustacea: Amphipoda)

2004 ◽  
Vol 84 (1) ◽  
pp. 171-178 ◽  
Author(s):  
Filipe O. Costa ◽  
Marina R. Cunha ◽  
Teresa Neuparth ◽  
Christopher W. Theodorakis ◽  
M. Helena Costa ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Taxonomic Identification

Sodium dodecyl sulphate as a rapid clearing agent for studying the hard parts of monogeneans and nematodes

2006 ◽  
Vol 80 (1) ◽  
pp. 87-90 ◽  
Author(s):  
W.L. Wong ◽  
W.B. Tan ◽  
L.H.S. Lim
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Simple Technique ◽  
Sodium Dodecyl ◽  
Taxonomic Identification ◽  
Conventional Methods ◽  
Hard Structures

AbstractHard structures of helminths have often been used for taxonomic identification but are usually not clearly defined when treated with conventional methods such as ammonium picrate-glycerin for monogeneans and glycerin for nematodes. The present study reports a rapid and simple technique to better resolve the hard parts of selected monogeneans and nematodes using 5–10% alkaline sodium dodecyl sulphate (SDS). In comparison with established methods, SDS-treated worms become more transparent. In monogeneans treated with SDS, clear details of the hooks, hook filaments, anchors, bars and the sclerotized copulatory organs could be observed. In SDS-treated nematodes, spicules and ornamentations of the buccal capsules could be clearly seen.

scholarly journals Application of Biotests in Cyanobacterial Extract Toxicity Assessment

2014 ◽  
Vol 40 (3) ◽  
pp. 115-121 ◽  
Author(s):  
Anna Sierosławska ◽  
Anna Rymuszka ◽  
Tadeusz Skowroński
Keyword(s):  
Acute Toxicity ◽  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Tetrahymena Thermophila ◽  
Toxicity Assessment ◽  
Taxonomic Identification ◽  
Dam Reservoir ◽  
Extract Concentration ◽  
Initial Extract ◽  
Toxicity Unit

Abstract The aim of the study was to determine the toxicity of the extract obtained from the cyanobacterial cells derived from the waters of Zemborzycki dam reservoir with use of a battery of biotests. The taxonomic identification of the bloom-forming cyanobacteria revealed high abundance of Aphanizomenon flos-aquae and Dolichospermum spp. (Anabaena spp.) and in a lower degree of Microcystis aeruginosa and Planktothrix agardhii. In the extract obtained from concentrated cyanobacterial cells, hepatotoxin microcystin-LR at a concentration of 22.89 ± 3.74 μg/L and neurotoxin Antx-a at 13.02 ± 0.01 μg/L have been detected. Toxicity of the extract was evaluated with the following assays: Daphtoxkit F magna with the crustacean Daphnia magna, Thamnotoxkit F with the crustacean Thamnocephalus platyurus, Rotoxkit F with the rotifer Brachionus calyciflorus and Protoxkit F with ciliate Tetrahymena thermophila. The most sensitive organism among all studied was T. platyurus for which EC50 was estimated to be 1.2% of the initial extract concentration. On the basis of the highest obtained value of the toxicity unit (TU = 83) the studied sample was classified to the IV class, which is of high acute toxicity. Additionally, it was found that reactivity on cyanobacterial products differs greatly among organisms used in bioassays, which indicate the need for using a set of biotests.

scholarly journals COMPARATIVE ANATOMY AND HISTOCHEMISTRY OF FRUITS OF FOUR VARIETIES OF Hancornia speciosa GOMES (APOCYNACEAE)

2021 ◽  
Vol 8 (2) ◽  
pp. 31-41
Author(s):  
Dayana Figueiredo Abdalla ◽  
Indiara Nunes Mesquita Ferreira ◽  
Moemy Gomes de Moraes ◽  
Eli Regina Barboza de Souza
Keyword(s):  
Secondary Metabolites ◽  
Plant Species ◽  
Comparative Anatomy ◽  
Economic Importance ◽  
Taxonomic Identification ◽  
Anatomical Characteristics ◽  
Isolated Organs ◽  
The Family ◽  
Hancornia Speciosa ◽  
Botanical Varieties

Hancornia speciosa Gomes, known as mangaba or mangabeira, is a plant species of the family Apocynaceae with botanical varieties occurring in several regions of Brazil. The species is of considerable ecological and economic importance in the food, timber, latex and medicinal industries. Four different varieties of H. speciosa occur in Cerrado. Anatomical characteristics contribute to the taxonomic identification of plants, including those of isolated organs such as leaves, roots, stems, fruits and seeds. Thus, the present work investigated the anatomical characteristics and the location of the main classes of secondary metabolites of the fruits of H. speciosa var. pubescens, H. speciosa var. gardneri, H. speciosa var. speciosa and H. speciosa var. cuyabensis to assist in distinguishing the varieties. Fruits were collected, preserved and fixed following usual methods for anatomical and histochemical analyses. All varieties under study showed similarities in anatomical characteristics and in the distribution of metabolites in the fruits, with the exception of the anatomy of the exocarp, where the occurrence of trichomes differed among the varieties of H. speciosa.

scholarly journals Modeling of the immature stages of the species of Noctuidae associated with Physalis peruviana L.

2019 ◽  
Vol 72 (1) ◽  
pp. 8673-8684
Author(s):  
Sebastián Alfonso Guzmán Cabrera ◽  
Adelaida María Gaviria Rivera ◽  
Jhon Alveiro Quiroz Gamboa ◽  
Dario Antonio Castañeda Sánchez
Keyword(s):  
The Other ◽  
Agrotis Ipsilon ◽  
Plant Canopy ◽  
Taxonomic Identification ◽  
Immature Stages ◽  
Heliothis Subflexa ◽  
Peridroma Saucia ◽  
Fruit Crop ◽  
Ecological Characteristics ◽  
Physalis Peruviana

Physalis peruviana L. is currently the second fruit crop more exported of Colombia; however, the pests associated with the culture have been little studied which is important considering that some Noctuidae can cause a decrease of 20% in its production. In this research, the Noctuidae species related to P. peruviana were studied in three farms of La Unión, Antioquia, Colombia. Twelve sampling units, with 30- and 45-day transplanted plants, were distributed throughout the farms and sampled biweekly from March 1st to August 29th of 2014. In the plant canopy and the planted area, immature stages were registered, and statistic models were built in order to describe their trend. The taxonomic identification of adults was made by comparing with the Noctuidae collection of Museo Entomológico Francisco Luis Gallego at Universidad Nacional de Colombia – Sede Medellín, and by using taxonomic keys. Nine Noctuidae species were found in total. Six models were built, four oviposition models for Agrotis ipsilon and Spodoptera spp., Copitarsia decolora and Heliothis subflexa, Megalographa biloba, and Peridroma saucia; a model for larvae and pupae stages was built. The oviposition model for P. saucia was the more adjusted, with a Root mean squared predictive difference (RMSPD) of 0.84. The other studied models were suitable to describe the trend of the immature stages; except for M. biloba model. This research revealed the ecological characteristics of the Noctuidae species associated with the golden berry crop that affect its productivity. 

scholarly journals Detección de la presencia del ácaro (Aculops lycopersici) causante del bronceamiento del tomate (Lycoprsicon esculentum) en El Salvador, América Central

2016 ◽  
Vol 2 ◽  
pp. 49
Author(s):  
Leopoldo Serrano Cervantes
Keyword(s):  
Costa Rica ◽  
El Salvador ◽  
Central America ◽  
Taxonomic Identification ◽  
Review Of Literature ◽  
Plant Mortality ◽  
Tomato Plants ◽  
Aculops Lycopersici ◽  
The Tropics ◽  
First Time

In October 1989, microscopic specimens of (acaros) with symptoms of browning and plant mortality in young and older tomato plants from various locations in El Salvador were sent tó the entomologist Ing. Ronald Ochoa from Costa Rica. lng. Ronald Ochoa is associated with the Diagnostics Network of the Centro Agronomico Tropical de Investigation y Enseñanza (CATlE) based in Turrialba, Costa Rica. Microscopic examination of this material confirmed the taxonomic identification as Aculops lycopersici (Acariformes: Eriophyidae), suspected since May 1988 with the first observation and collection of samples. Abrief review of literature and a phone conversation with the specialist who identified the material, permits reporting for the first time of the presence of this species in El Salvador. This finding is a warning about this pest which could be apotential problem in the tropics of Central America. This paper discusses personal observations of some of the characteristics, habits and appearances of this species, small experiments, and it includes a review of literature pertinent to this subject

Wyniki oznaczeń taksonomicznych szczątków drewna

2020 ◽  
pp. 105-106
Author(s):  
Grzegorz Skrzyński
Keyword(s):  
Pinus Sylvestris ◽  
Human Activity ◽  
Scots Pine ◽  
Woody Plants ◽  
The Other ◽  
Taxonomic Identification ◽  
Forest Communities ◽  
Widespread Occurrence ◽  
Przeworsk Culture ◽  
High Share

A number of samples collected during exploration of archaeological features from the Przeworsk culture cremation cemetery were submitted for xylological examination. The samples contained poorly preserved charred remains of wood, which were subjected to taxonomic identification. Anthracological analyses allowed four taxa of woody plants to be identified, with the predominant share of remains belonging to Scots pine Pinus sylvestris. The high share of pine wood fragments may indicate selective acquisition of this species as a material for building funeral pyres. On the other hand, it may reflect the widespread occurrence of this species in the nearby forest communities, which were shaped by human activity.

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