Coinoculation of soybean plants with Bradyrhizobium japonicum and Trichoderma harzianum: Coexistence of both microbes and relief of nitrate inhibition of nodulation

ABSTRACT This study aimed to verify the effect of co-inoculation, association between Azospirillum brasilense and Bradyrhizobium japonicum bacteria, on soybean plants subjected to water deficit at two sowing dates. Two field experiments were conducted at the Universidade Federal de Tocantins, campus of Palmas, Brazil, in 2016. The experimental design was randomized blocks in a split-split-plot arrangement with four repetitions, where the plots consisted of two irrigation depths (100 and 25% of crop evapotranspiration - ETc), the subplots was composed of two methods of inoculant application (inoculation with Bradyrhizobium japonicum and co-inoculation with Azospirillum brasilense + Bradyrhizobium japonicum) and the sub-subplots comprised two soybean cultivars (TMG 132 and ANTA 82). The cultivars responded differently to the sowing dates. Co-inoculation did not influence grain yield under full irrigation conditions (100% ETc), in neither cultivar evaluated. However, under the water deficit condition (25% ETc), the grain yield of the cultivar TMG 132 increased 77.20%, indicating that there are different responses of interaction between Azospirillum brasilense, plant genotype and sowing dates.

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Test of Host Sanction Hypothesis in Soybean Plants Co-inoculated with Nitrogen Fixing and Non-fixing Bradyrhizobium japonicum

Journal of Scientific Research and Reports ◽

10.9734/jsrr/2015/15510 ◽

2015 ◽

Vol 6 (2) ◽

pp. 99-108 ◽

Cited By ~ 1

Author(s):

Diana Marco ◽

Chouhra Talbi ◽

Eulogio Bedmar

Keyword(s):

Bradyrhizobium Japonicum ◽

Nitrogen Fixing ◽

Soybean Plants

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Nodulation and Development of Soybean Submitted to Inoculation With Bradyrhizobium japonicum and Phosphorus Doses

Journal of Agricultural Science ◽

10.5539/jas.v10n12p321 ◽

2018 ◽

Vol 10 (12) ◽

pp. 321 ◽

Cited By ~ 1

Author(s):

Erica Chaves ◽

Rubson da Costa Leite ◽

Thalita Rodrigues Silva ◽

Thayny Alves Viana ◽

Tatiane de Sousa Cruz ◽

...

Keyword(s):

Nitrogen Fixation ◽

Experimental Design ◽

Bradyrhizobium Japonicum ◽

Phosphate Fertilizer ◽

Dry Mass ◽

Root Number ◽

Phosphate Fertilization ◽

Soybean Plants ◽

Biological Nitrogen ◽

Plant Dry Mass

Among the several factors that may influence nodulation and the efficiency of biological nitrogen fixation for soybean plants, nutrient availability is among the most important. This study aimed to evaluate the inoculation with Bradyrhizobium japonicum and doses of phosphorus on the development of soybean in a Vertisol, in Tocantins. The experimental design was completely randomized in a 4 × 2 factorial scheme, with four replications. Four doses of phosphate fertilization (0, 100, 200, and 300 kg ha-1 P2O5) were studied, combined with two inoculation treatments with Bradyrhizobium japonicum (inoculated and not inoculated). The following variables were evaluated: plant height, stem diameter, nodules per plant, dry mass of nodules, dry mass of plant, dry mass of root, number of pods and number of grains per pod. Under greenhouse conditions and soil with good availability of phosphorus, there is no influence of the doses on the inoculation with Bradyrhizobium japonicum. Soils with good availability of phosphorus have low response to the application of phosphate fertilizer.

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Curled root hairs are a site of entry for Bradyrhizobium infecting hydroponically grown soybean plants with mature root systems

Canadian Journal of Botany ◽

10.1139/b88-098 ◽

1988 ◽

Vol 66 (4) ◽

pp. 683-686 ◽

Cited By ~ 1

Author(s):

Jeanne M. L. Selker ◽

John Imsande ◽

Eldon H. Newcomb

Keyword(s):

Glycine Max ◽

Root Hair ◽

Bradyrhizobium Japonicum ◽

Root Hairs ◽

Root Systems ◽

Glycine Max L ◽

Soybean Plants ◽

A Site ◽

Hydroponically Grown ◽

Mature Root

Early emergent nodules on roots of hydroponically grown soybean plants (Glycine max (L.) Merr.) were sectioned serially to locate the site of infection by Bradyrhizobium japonicum. The plants had been inoculated only after their root systems had produced numerous higher order branches. The hydroponic solutions contained all required nutrients, including either a suboptimal concentration of nitrate (0.5 mM) or an excess of nitrate (4.0 mM). In all six nodules examined, three with suboptimal nitrate and three with excess nitrate, we found a centrally located root hair containing an infection thread. We conclude that mature root systems of soybean grown in aqueous culture can undergo infection through root hairs in the way that is typical of young seedlings grown either in pots of vermiculite or pouches.

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MIGRATION OF BIOGENIC ELEMENTS COMPOUNDS IN THE APPLICATION OF COMPLEX INOCULANT FOR SOYBEAN

Agriciltural microbiology ◽

10.35868/1997-3004.24.24-28 ◽

2016 ◽

Vol 24 ◽

pp. 24-28

Author(s):

S. F. Kozar ◽

T. A. Yevtushenko ◽

L. V. Potapenko ◽

Ye. P. Chmel ◽

V. P. Horban

Keyword(s):

Bradyrhizobium Japonicum ◽

Azospirillum Brasilense ◽

Water Soluble ◽

Biogenic Elements ◽

Mineral Fertilizers ◽

Biometric Parameters ◽

Magnesium Compounds ◽

Calcium Magnesium ◽

Soybean Plants ◽

Nitrogen Phosphorus

In lysimetric experiment with soybean, the effect of complex inoculants based on Bradyrhizobium japonicum and Azospirillum brasilense on the vertical migration of biogenic elements have been studied. When growing without application of mineral fertilizers, bacterization helped to reduce losses of nitrogen, phosphorus, potassium, calcium, magnesium compounds, as well as moisture and water-soluble humus. Also reduction in loss of biogenic elements compounds under the action of inoculants on the background of N30 was found, but bacterization not affect the loss of moisture and humus. It was shown that complex inoculants affect biometric parameters of soybean plants and enhances the productivity of this culture.

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THE INFLUENCE OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE ON THE VERTICAL MIGRATION OF NUTRIENTS AT SOYBEAN CULTIVATION

Agriciltural microbiology ◽

10.35868/1997-3004.21.39-43 ◽

2015 ◽

Vol 21 ◽

pp. 39-43

Author(s):

S. F. Kozar ◽

I. M. Pyschur ◽

V. M. Nesterenko

Keyword(s):

Chlorophyll Content ◽

Vertical Migration ◽

Bradyrhizobium Japonicum ◽

Azospirillum Brasilense ◽

Water Soluble ◽

Nitrogen Fixing ◽

Nitrogen Fixing Bacteria ◽

Joint Application ◽

Calcium And Magnesium ◽

Soybean Plants

The paper presents the research results of pre-sowing seeds bacterization with nitrogen fixing bacteria Bradyrhizobium japonicum and Azospirillum brasilense influence on the loss of moisture, water soluble humus and nutrients. It was shown that seeds bacterization reduces the leaching intensity of nitrates, phosphorus, potassium, calcium and magnesium. Moreover, the least losses of nutrients were observed in a variants with joint application of both studied nitrogen fixing microorganisms. It was noted that seeds bacterization with B. japonicum and A. brasilense had promoted increase of chlorophyll content in the leaves of soybean plants. The highest yield was observed in the variant with the joint use of rhizobia and azospirillum.

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Role of Bradyrhizobium japonicum and Trichoderma spp. in the control of root rot disease of soybean

Acta Mycologica ◽

10.5586/am.1995.003 ◽

2014 ◽

Vol 30 (1) ◽

pp. 35-40 ◽

Cited By ~ 1

Author(s):

Syed Ehteshamul-Haque ◽

Abdul Ghaffar

Keyword(s):

Grain Yield ◽

Rhizoctonia Solani ◽

Trichoderma Harzianum ◽

Root Rot ◽

Bradyrhizobium Japonicum ◽

Seed Treatment ◽

Trichoderma Spp ◽

Root Rot Disease ◽

Rot Disease

Seed treatment of soybean with Bndyrhizobium japonicum, Trichoderma harzianum, T. viride, T. hamatum, T. koningii and T. pseudokoningii significantly controlled the infection of 30-day-old seedlingsby Maerophomina phaseolina, Rhizoctonia solani and Fusarium spp. In 60-day-old plants Trichoderma spp.. and B. japonicum inhibited the grouth of R. solani and Fusarium spp., whereas the use of B. japonicum (TAL-102) with T. harzianum. T. viride, T. koningii and T. pseudokoningii controlled the infection by M. phaseolina. Greater grain yield was recorded when B. japonium (TAI-102) was used with T. hamatum.

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EFEKTIVITAS STRAIN BRADYRHIZOBIUM JAPONICUM YANG DIISOLASI DARI BEBERAPA LOKASI DI ENDE TERHADAP PEMBENTUKAN NODUL DAN PENAMBATAN NITROGEN PADA TANAMAN KEDELAI (GLYCINE MAX L.)

AGRICA ◽

10.37478/agr.v3i2.501 ◽

2020 ◽

Vol 3 (2) ◽

pp. 138-150

Author(s):

Kristina Erniyani

Keyword(s):

Bradyrhizobium Japonicum ◽

Symbiotic Nitrogen Fixation ◽

Dry Weight ◽

Treatment Groups ◽

Nitrogen Levels ◽

Commercial Inoculant ◽

Glycine Max L ◽

Soybean Plants ◽

Bradyrhizobium Spp ◽

Bradyrhizobium Inoculation

Bacteria Bradyrhizobium spp. From nodules on soybean plants, bind nitrogen from the air and make it freely available to plants. Amount of nitrogen fixed depends on the ability of a strain to form nodules, nodule effectiveness and environmental conditions. On land – land that had been planted with soybeans, inoculation with Bradyrizhobium bacteria are often not required. To test these lands in the district of Ende, NTT has conducted an experiment with a factor in the design of Randomized Complete with nine treatment groups. The treatment consists of (i) inoculation with the soybean crop in the ground former Ekoae (R1), (ii) inokulasi with soil former soybean plants in Ndona (R2), (iii) inoculation of soybean plants with the soil used in Flores (R3), (iv) Brady rhizobium isolates from soybean nodules in Ekoae (R4), (v) Bradyrhizobium isolates from nodules of soybean plants is in Ndona (R5), (v) Bradyrhozobium isolates from nodules of soybean plants at Flores (R6) (vii) a commercial inoculant legin (L), (viii) fertilizer nitrogen (N), and (ix) Control (K). all treatments were repeated 4 times. Land used in these experiments is the land of Ndona. The experiment was carried out since faculty, Udayana University. The highest percentage of effective nodules on the treatment Bradyrhizobium isolates from sybean nodules in Ndona (R5). Total nitrogen levels at the age of 45 HST in the highest in soybean plants that received 100 kg ha-1 urea and soybeans that have a desolate Bradyrhizobium inoculation of soybean nodules in Ndona (R). Efficiency and levels of symbiotic nitrogen fixation result obtained highest in soybean plants that received an inoculation Bradyrhizobium diisolat soybean nodules in Ndona (R5). Oven dry weight of plants that from the age of 45 HST on all isolates are equivalent to the oven-dry weight of plants at the fertilization of 100 kg ha- 1 urea. The result shoed that the land – the land of Ekoae, Ndona and Fkres have high Bradirhizobium contain bacteria. Bacteria Bradyrhizobium cp. Most numerous in the isolates from soybean nodules in Ndona (R5) the most efficient from effective nodules is 82.26% and as high as the 0.52% N fixate. These results indicate that isolates from Ndona Bradyrhizobiun veeb able to form effective nonudel on yoy beans grown in soil from Ndona. The result also indicates inoculation with a commercial inoculant was obtained not on land – land planted with soybeans used

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