scholarly journals EFEKTIVITAS STRAIN BRADYRHIZOBIUM JAPONICUM YANG DIISOLASI DARI BEBERAPA LOKASI DI ENDE TERHADAP PEMBENTUKAN NODUL DAN PENAMBATAN NITROGEN PADA TANAMAN KEDELAI (GLYCINE MAX L.)

AGRICA ◽  
2020 ◽  
Vol 3 (2) ◽  
pp. 138-150
Author(s):  
Kristina Erniyani
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Symbiotic Nitrogen Fixation ◽  
Dry Weight ◽  
Treatment Groups ◽  
Nitrogen Levels ◽  
Commercial Inoculant ◽  
Glycine Max L ◽  
Soybean Plants ◽  
Bradyrhizobium Spp ◽  
Bradyrhizobium Inoculation

Bacteria Bradyrhizobium spp. From nodules on soybean plants, bind nitrogen from the air and make it freely available to plants. Amount of nitrogen fixed depends on the ability of a strain to form nodules, nodule effectiveness and environmental conditions. On land – land that had been planted with soybeans, inoculation with Bradyrizhobium bacteria are often not required. To test these lands in the district of Ende, NTT has conducted an experiment with a factor in the design of Randomized Complete with nine treatment groups. The treatment consists of (i) inoculation with the soybean crop in the ground former Ekoae (R1), (ii) inokulasi with soil former soybean plants in Ndona (R2), (iii) inoculation of soybean plants with the soil used in Flores (R3), (iv) Brady rhizobium isolates from soybean nodules in Ekoae (R4), (v) Bradyrhizobium isolates from nodules of soybean plants is in Ndona (R5), (v) Bradyrhozobium isolates from nodules of soybean plants at Flores (R6) (vii) a commercial inoculant legin (L), (viii) fertilizer nitrogen (N), and (ix) Control (K). all treatments were repeated 4 times. Land used in these experiments is the land of Ndona. The experiment was carried out since faculty, Udayana University. The highest percentage of effective nodules on the treatment Bradyrhizobium isolates from sybean nodules in Ndona (R5). Total nitrogen levels at the age of 45 HST in the highest in soybean plants that received 100 kg ha-1 urea and soybeans that have a desolate Bradyrhizobium inoculation of soybean nodules in Ndona (R). Efficiency and levels of symbiotic nitrogen fixation result obtained highest in soybean plants that received an inoculation Bradyrhizobium diisolat soybean nodules in Ndona (R5). Oven dry weight of plants that from the age of 45 HST on all isolates are equivalent to the oven-dry weight of plants at the fertilization of 100 kg ha- 1 urea. The result shoed that the land – the land of Ekoae, Ndona and Fkres have high Bradirhizobium contain bacteria. Bacteria Bradyrhizobium cp. Most numerous in the isolates from soybean nodules in Ndona (R5) the most efficient from effective nodules is 82.26% and as high as the 0.52% N fixate. These results indicate that isolates from Ndona Bradyrhizobiun veeb able to form effective nonudel on yoy beans grown in soil from Ndona. The result also indicates inoculation with a commercial inoculant was obtained not on land – land planted with soybeans used

scholarly journals DEVELOPMENT OF PIGEON PEA INOCULATED WITH RHIZOBIUM ISOLATED FROM COWPEA TRAP HOST PLANTS

2016 ◽  
Vol 29 (4) ◽  
pp. 789-795 ◽  
Author(s):  
SALOMÃO LIMA GUIMARÃES ◽  
LAURA CRISTINA REZENDE DAS NEVES ◽  
EDNA MARIA BONFIM-SILVA ◽  
DANIELA TIAGO DA SILVA CAMPOS
Keyword(s):  
Nitrogen Fertilization ◽  
Dry Weight ◽  
Pigeon Pea ◽  
Index Number ◽  
Randomized Design ◽  
Rhizobium Strains ◽  
Commercial Inoculant ◽  
Red Latosol ◽  
Inoculation Treatment ◽  
Bradyrhizobium Spp

ABSTRACT Pigeon pea is an important protein source grown in several tropical and sub-tropical countries, and is considered a multi-purpose plant that is resistant to the conditions of the Brazilian Cerrado. Among the possible uses for cowpea, its use as a green manure, increasing soil nitrogen content through the association with diazotrophic bacteria, generically known as rhizobia, is noteworthy. The present work aimed to evaluate the efficiency of Rhizobium strains isolated from cowpea plants in the development of pigeon peas cultured in Red Latosol. The experiment was conducted in a greenhouse, using a completely randomized design with seven treatments and four replications. Treatments consisted of inoculation with four Rhizobium strains (MT8, MT15, MT16, and MT23) and one commercial inoculant comprising Bradyrhizobium spp. strains BR 2801 and BR 2003. There were two controls, one absolute (without inoculation or nitrogen fertilization) and the other with nitrogen fertilization. Each experimental plot consisted of an 8-dm3 vase containing three plants. Analyzed variables included plant height, SPAD index, number and dry weight of nodules, and shoot and root dry masses. Pigeon peas responded significantly to inoculation treatment, since all the plants inoculated with Rhizobium strains isolated from cowpea strains showed results similar to plants in the nitrogen control and commercial inoculant treatments. This demonstrates a favorable plant-bacteria interaction, which can be utilized as an alternative nitrogen source for pigeon peas.

Curled root hairs are a site of entry for Bradyrhizobium infecting hydroponically grown soybean plants with mature root systems

1988 ◽  
Vol 66 (4) ◽  
pp. 683-686 ◽  
Author(s):  
Jeanne M. L. Selker ◽  
John Imsande ◽  
Eldon H. Newcomb
Keyword(s):  
Glycine Max ◽  
Root Hair ◽  
Bradyrhizobium Japonicum ◽  
Root Hairs ◽  
Root Systems ◽  
Glycine Max L ◽  
Soybean Plants ◽  
A Site ◽  
Hydroponically Grown ◽  
Mature Root

Early emergent nodules on roots of hydroponically grown soybean plants (Glycine max (L.) Merr.) were sectioned serially to locate the site of infection by Bradyrhizobium japonicum. The plants had been inoculated only after their root systems had produced numerous higher order branches. The hydroponic solutions contained all required nutrients, including either a suboptimal concentration of nitrate (0.5 mM) or an excess of nitrate (4.0 mM). In all six nodules examined, three with suboptimal nitrate and three with excess nitrate, we found a centrally located root hair containing an infection thread. We conclude that mature root systems of soybean grown in aqueous culture can undergo infection through root hairs in the way that is typical of young seedlings grown either in pots of vermiculite or pouches.

scholarly journals Development and Application of a Multilocus Sequence Analysis Method for the Identification of Genotypes Within Genus Bradyrhizobium and for Establishing Nodule Occupancy of Soybean (Glycine max L. Merr)

2012 ◽  
Vol 25 (3) ◽  
pp. 321-330 ◽  
Author(s):  
Peter van Berkum ◽  
Patrick Elia ◽  
Qijian Song ◽  
Bertrand D. Eardly
Keyword(s):  
Allelic Variation ◽  
Geographic Origin ◽  
Geographic Location ◽  
Culture Collection ◽  
Nodule Occupancy ◽  
Analysis Method ◽  
Glycine Max L ◽  
Soybean Plants ◽  
Chromosomal Loci ◽  
Bradyrhizobium Spp

A multilocus sequence typing (MLST) method based on allelic variation of seven chromosomal loci was developed for characterizing genotypes (GT) within the genus Bradyrhizobium. With the method, 29 distinct multilocus GT were identified among 190 culture collection soybean strains. The occupancy of 347 nodules taken from uninoculated field-grown soybean plants also was determined. The bacteroid GT were either the same as or were closely related to GT identified among strains in the culture collection. Double-nodule occupancy estimates of 2.9% were much lower than values published based on serology. Of the 347 nodules examined, 337 and 10 were occupied by Bradyrhizobium japonicum and B. elkanii, respectively. The collection strains within the species B. japonicum and B. elkaniialso were compared with Bradyrhizobium cultures from other legumes. In many cases, the observed GT varied more according to their geographic origin than by their trap hosts of isolation. In other cases, there were no apparent relationships with either the legume or geographic source. The MLST method that was developed should be a useful tool in determining the influence of geographic location, temperature, season, soil type, and host plant cultivar on the distribution of GT of Bradyrhizobium spp.

scholarly journals Soybean response to starter nitrogen and Bradyrhizobium inoculation on a Cerrado oxisol under no-tillage and conventional tillage systems

2003 ◽  
Vol 27 (1) ◽  
pp. 81-87 ◽  
Author(s):  
I. C. Mendes ◽  
M. Hungria ◽  
M. A. T. Vargas
Keyword(s):  
Cropping Systems ◽  
Dry Weight ◽  
Conventional Tillage ◽  
N Fertilizer ◽  
No Tillage ◽  
Flowering Stage ◽  
Legume Crop ◽  
Glycine Max L ◽  
N Rates ◽  
Bradyrhizobium Inoculation

In Brazil, Bradyrhizobium inoculation has successfully replaced the use of N fertilizer on soybean [Glycine max (L) Merr.] crops. However, with the expansion of no-tillage cropping systems in the Cerrados region, the idea that it is necessary to use small N rates at the sowing to overcome problems related with N immobilization has become widespread, mainly when soybean is cultivated after a non-legume crop. In this study we examined soybean response to small rates of N fertilizer under no-tillage (NT) and conventional tillage (CT) systems. Four experiments (a completely randomized block with five replicates) were carried out in a red yellow oxisol, during the periods of 1998/1999 and 1999/ 2000, under NT and CT. The treatments consisted of four urea rates (0, 20, 30 and 40 kg ha-1 N). All treatments were inoculated with Bradyrhizobium japonicum strains SEMIA 5080 and SEMIA 5079, in the proportion 1 kg of peat inoculant (1,5 x 10(9) cells g-1) per 50 kg of seeds. In both experiments, soybean was cultivated after corn and the N fertilizer was band applied at sowing. In all experiments, N rates promoted reductions of up to 50 % in the nodule number at 15 days after the emergence. Regardless of the management system, these reductions disappeared at the flowering stage and there was no effect of N rates on either the number and dry weight of nodules or on soybean yields. Therefore, in the Brazilian Cerrados, when an efficient symbiosis is established, it is not necessary to apply starter N rates on soybean, even when cultivated under notillage systems.

scholarly journals Perbedaan Kebutuhan Nitrogen untuk Produksi Kedelai di Tanah Mineral dan Mineral Bergambut Dengan Budidaya Jenuh Air

2016 ◽  
Vol 35 (3) ◽  
pp. 217 ◽  
Author(s):  
Bachtiar ◽  
Munif Ghulamahdi ◽  
Maya Melati ◽  
Dwi Guntoro ◽  
Atang Sutandi
Keyword(s):  
Block Design ◽  
Nitrogen Concentration ◽  
Mineral Soil ◽  
Dry Weight ◽  
Fertilizer Application ◽  
Land Type ◽  
Randomized Block Design ◽  
Nitrogen Concentrations ◽  
Glycine Max L ◽  
Soybean Plants

Development of soybean (Glycine max (L) Merr.) in tidal land are faced with problems physical, chemical and biological soil properties, such as high organic matter, high soil acidity, toxicity of Fe and Al, and deficiency of nutrients N, P , K, Ca and Mg. N content is high (> 0.51%) but with low availability.  The research objective is to determine the dose and timing of N, P and K application in accordance with the needs of soybean plants to have optimally growth and production in mineral and peaty mineral soil in tidal land. The research was conducted in mineral and peaty mineral soil of tidal land type C and B, District of Tanjung Lago, Banyuasin Regency, Province of South Sumatra from April to August 2014. The model is linear using split plots in a randomized block design. Varieties of Willis and Tanggamus were used for nitrogen application experiment.  The time of fertilizer application is at 2, 3 and 4 weeks after planting (WAP), 2, 3, 4 and 5 WAP, and 2, 3, 4, 5 and 6 WAP. The concentration of nitrogen is 7,5; 10; 12,5 and 15 g/l water with spraying volume of 400 l/ha.  In the mineral soil, dry weight of nodules and Willis biomass increased with increasing frequency time of fertilization, otherwise Tanggamus more fluctuating and declined at higher frequency of fertilization. Wilis variety generating the highest production of 3,5 ton/ha.  In the peaty mineral soil, dry weight of nodules and biomass were not significant.  Willis productivity tends to decrease with increasing nitrogen concentrations.  Tanggamus productivity tends to increase with increasing nitrogen concentration at all level of time fertilization.  Tanggamus tend to generate higher productivity of 3,2 ton/ha.

scholarly journals Pertumbuhan Batang Pada Beberapa Varietas Kedelai (Glycine max (L.) Merr) Terhadap Jenis Tanah Aluvial, Regosol Dan Latosol

2020 ◽  
Vol 13 (01) ◽  
pp. 24-32
Author(s):  
Kurniawan M.R.D ◽  
P. S. Ajiningrum
Keyword(s):  
Block Design ◽  
Dry Weight ◽  
Stem Length ◽  
Test Results ◽  
Hypocotyl Length ◽  
Randomized Block Design ◽  
Significant Difference ◽  
Different Types ◽  
Glycine Max L ◽  
Soybean Plants

This study aims to determine the growth of 10 superior soybean varieties planted in 3 different types of soil (alluvial, regosol and latosol) and to find out whether the 3 types of soil give an effect to the growth of 10 superior soybean varieties viewed from 4 parameters (hypocotyl length, length epicotiles, stem length and stem dry weight).This study used a Randomized Block Design (RBD) consisting of 10 varieties of soybean plants (anjasmoro, argomulyo, detam 1, detam 3 prida, detam 4 prida, detap 1, devon 1, devon 2, yellow gepak and deja 1) and 3 types of soil (alluvial, regosol, and latosol) was repeated 2 times. Data were analyzed using the two-way ANAVA 5% test and the 5% DMRT test. The results showed a significant difference in soybean growth. The best soybean varieties in hypocotyl length are Devon 2, in epicotile length are Anjasmoro, Devon 1 and Devon 2, the stem length and dry weight of the stem are Detap 1. The DMRT test results state that all three soil types (alluvial, regosol and latosol) have influence on the parameters of hypocotyl length and epicotyl length, whereas the parameters of stem length and plant dry weight have no effect.   Keywords: Soybean, Variety, Growing test  

scholarly journals Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy  

2022 ◽  
Author(s):  
Dragana Miljakovic ◽  
Jelena Marinković ◽  
Maja Ignjatov ◽  
Dragana Milosević ◽  
Zorica Nikolić ◽  
...  
Keyword(s):  
Nitrogen Content ◽  
Bradyrhizobium Japonicum ◽  
Dry Weight ◽  
Soil Conditions ◽  
Nodule Occupancy ◽  
Enterobacterial Repetitive Intergenic Consensus ◽  
Shoot Dry Weight ◽  
Soybean Nodule ◽  
Commercial Inoculant ◽  
Selection For

The competitiveness of Bradyrhizobium japonicum inoculation strain against indigenous rhizobia was examined in a soil pot experiment. The effect of inoculation strain was evaluated under different soil conditions: with or without previously grown soybean and applied commercial inoculant. Molecular identification of inoculation strain and investigated rhizobial isolates, obtained from nodules representing inoculated treatments, was performed based on 16S rDNA and enterobacterial repetitive intergenic consensus (ERIC) sequencing. Inoculation strain showed a significant effect on the investigated parameters in both soils. Higher nodule occupancy (45% vs. 18%), nodule number (111% vs. 5%), nodule dry weight (49% vs. 9%), shoot length (15% vs. 7%), root length (31% vs. 13%), shoot dry weight (34% vs. 11%), shoot nitrogen content (27% vs. 2%), and nodule nitrogen content (9% vs. 5%) was detected in soil without previously grown soybean and applied commercial inoculant. Soil had a significant effect on the shoot, root and nodule nitrogen content, while interaction of experimental factors significantly altered dry weight and nitrogen content of shoots, roots and nodules, as well as number of nodules. Nodulation parameters were significantly related with shoot dry weight, shoot and nodule nitrogen content. Symbiotic performance of inoculation strains in the field could be improved through co-selection for their competitiveness and effectiveness.  

Alterations in internal partitioning of carbon in soybean plants in response to nitrogen stress

1984 ◽  
Vol 62 (3) ◽  
pp. 501-508 ◽  
Author(s):  
Thomas W. Rufty Jr. ◽  
C. David Raper Jr. ◽  
Steven C. Huber
Keyword(s):  
Carbon Allocation ◽  
Day Treatment ◽  
Dry Weight ◽  
Nitrogen Supply ◽  
Growth Potential ◽  
Nitrogen Partitioning ◽  
Sink Strength ◽  
Reduced Nitrogen ◽  
Glycine Max L ◽  
Soybean Plants

Alterations in internal partitioning of carbon were evaluated in plants exposed to limited nitrogen supply. Vegetative, nonnodulated soybean plants (Glycine max (L.) Merrill, ‘Ransom’) were grown for 21 days with 1.0 mM[Formula: see text] and then exposed to solutions containing 1.0, 0.1, or 0.0 mM[Formula: see text] for a 25-day treatment period. In nitrogen-limited plants, there were decreases in emergence of new leaves and in the expansion rate and final area at full expansion of individual leaves. As indicated by alterations in accumulation of dry weight, a larger proportion of available carbon in the plant was partitioned to the roots with decreased availability of nitrogen. Partitioning of reduced nitrogen to the root also was increased and, in plants devoid of an external nitrogen supply, considerable redistribution of reduced nitrogen from leaves to the root occurred. The general decrease in growth potential and sink strength for nutrients in leaves of nitrogen-limited plants suggested that factors other than simply availability of nitrogen likely were involved in the restriction of growth in the leaf canopy and the associated increase in carbon allocation to the roots.

scholarly journals Identification and use of actinomycetes for enhanced nodulation of soybean co-inoculated with Bradyrhizobium japonicum

2003 ◽  
Vol 49 (8) ◽  
pp. 483-491 ◽  
Author(s):  
A K Gregor ◽  
B Klubek ◽  
E C Varsa
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Antibiotic Sensitivity ◽  
Nodule Occupancy ◽  
Antibiotic Resistant ◽  
Glycine Max L ◽  
Resistant Strains ◽  
Soybean Plants ◽  
Population Sensitivity ◽  
Germinated Soybean ◽  
Streptomyces Kanamyceticus

The utilization of actinomycetes as potential soybean (Glycine max (L.)) co-inoculants was evaluated. Soil samples from Carbondale and Belleville, Ill., were used to inoculate pre-germinated soybean plants to determine antibiotic sensitivity in the native Bradyrhizobium japonicum population. Sensitivity was in the order kanamycin > tetracycline > oxytetracycline > rifampicin > neomycin. Antagonism by five actinomycete cultures toward seven test strains of B. japonicum was also assessed. The ranking average inhibition (across all seven B. japonicum strains) by these actino mycetes was Streptomyces kanamyceticus = Streptomyces coeruleoprunus > Streptomyces rimosus > Streptomyces sp. > Amy colatopsis mediterranei. Ten antibiotic combinations were used to isolate antibiotic-resistant mutants of B. japonicum I-110 and 3I1B-110 via successive cycles of mutation. Eighty-one antibiotic-resistant strains were isolated and tested for symbiotic competency; nine of which were selected for further characterization in a greenhouse pot study. Few differences in nodule number were caused by these treatments. Nodule occupancy varied from 0% to 18.3% when antibiotic-resistant strains of B. japonicum were used as the sole inoculants. However, when three mutant strains of B. japonicum were co-inoculated with S. kanamyceticus, significant increases in nodule occupancy (up to 55%) occurred. Increases in shoot nitrogen composition (27.1%–40.9%) were also caused by co-inoculation with S. kanamyceticus. Key words: Bradyrhizobium japonicum, Streptomyces kanamyceticus, indigenous bradyrhizobia, co-inoculation, nodule occupancy.

The nitrate-tolerant symbiosis of Glycine max (L.) Merr. nts382 and Bradyrhizobium japonicum is also tolerant of ammonium

2000 ◽  
Vol 77 (10) ◽  
pp. 1432-1438
Author(s):  
J Kevin Vessey ◽  
Bert Luit
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Continuous Flow ◽  
Dry Weight ◽  
Hydroponic Culture ◽  
Wild Type ◽  
Whole Plant ◽  
In The Wild ◽  
Glycine Max L ◽  
Wild Type Parent

Previously, we have shown that the Glycine max (L.) Merr. -Bradyrhizobium japonicum symbiosis is very sensitive to inhibition by NH4+. The current study addresses whether the supernodulating soybean mutant, nts382, which is known to be tolerant of NO3-, is also tolerant of NH4+. The nts382 mutant and its wild-type parent, Bragg, were grown in continuous-flow hydroponic culture in the presence of 0, 0.25, 0.5, or 1.0 mM 15N-enriched NH4+. Plants were harvested at 14, 21, and 28 days after inoculation. Both cultivars had the highest dry weight (DW) at each harvest date when grown on 0.25 mM NH4+. At 0.25 mM NH4+, whole plant DW increased by 5.3- and 3.2-fold in Bragg and nts382, respectively, compared with the 0.0 mM NH4+ control by the end of the experiment. As expected, whole-plant nodulation (nodules per plant), DW-specific nodulation (nodules per gram root dry weight), and nodule DW were severely inhibited in Bragg at all levels of NH4+. However, in nts382, whole-plant nodulation was not affected by NH4+ treatment, and nodule DW increased by as much as fivefold. Whereas DW-specific nodulation decreased by 94% in Bragg, this parameter decreased by only 52% in the nts382 mutant. Likewise, while the nitrogen derived from the atmosphere decreased by approximately 40% in NH4+-supplied Bragg, it increased 2.8-fold at 0.25 and 0.5 mM NH4+ in nts382. This study demonstrates that both nodulation and N2 fixation in nts382 are more tolerant of NH4+ than in the wild-type Bragg.

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