High-efficient production of fructo-oligosaccharides from inulin by a two-stage bioprocess using an engineered Yarrowia lipolytica strain

2017 ◽  
Vol 173 ◽  
pp. 592-599 ◽  
Author(s):  
Yao-Zu Han ◽  
Chao-Chao Zhou ◽  
Yan-Yan Xu ◽  
Jing-Xin Yao ◽  
Zhe Chi ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Efficient Production ◽  
Two Stage ◽  
High Efficient ◽  
Lipolytica Strain

scholarly journals Engineering of Yarrowia lipolytica transporters for high-efficient production of biobased succinic acid from glucose

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Zhennan Jiang ◽  
Zhiyong Cui ◽  
Ziwei Zhu ◽  
Yinghang Liu ◽  
Ya-jie Tang ◽  
...  
Keyword(s):  
Succinic Acid ◽  
Yarrowia Lipolytica ◽  
Tricarboxylic Acid Cycle ◽  
Efficient Production ◽  
Fed Batch ◽  
Metabolic Intermediate ◽  
High Efficient ◽  
Fed Batch Fermentation ◽  
Ph Conditions ◽  
Powerful Strategy

Abstract Background Succinic acid (SA) is a crucial metabolic intermediate and platform chemical. Development of biobased processes to achieve sustainable SA production has attracted more and more attention in biotechnology industry. Yarrowia lipolytica has a strong tricarboxylic acid cycle and tolerates low pH conditions, thus making it a potential platform for SA production. However, its SA titers in glucose media remain low. Results In this study, we screened mitochondrial carriers and C4-dicarboxylic acid transporters to enhance SA secretion in Y. lipolytica. PGC62-SYF-Mae strain with efficient growth and SA production was constructed by optimizing SA biosynthetic pathways and expressing the transporter SpMae1. In fed-batch fermentation, this strain produced 101.4 g/L SA with a productivity of 0.70 g/L/h and a yield of 0.37 g/g glucose, which is the highest SA titer achieved using yeast, with glucose as the sole carbon resource. Conclusion Our results indicated that transporter engineering is a powerful strategy to achieve the efficient secretion of SA in Y. lipolytica, which will promote the industrial production of bio-based SA.

Highly efficient production of FAMEs and β-farnesene from a two-stage biotransformation of waste cooking oils

2019 ◽  
Vol 199 ◽  
pp. 112001 ◽  
Author(s):  
Jiajia Lv ◽  
Yuefei Wang ◽  
Chengyu Zhang ◽  
Shengping You ◽  
Wei Qi ◽  
...  
Keyword(s):  
Efficient Production ◽  
Two Stage ◽  
Highly Efficient ◽  
Waste Cooking Oils ◽  
Cooking Oils

scholarly journals Blood Immunological and Biochemical Indicators in Turkey Hens Fed Diets With a Different Content of the Yeast Yarrowia Lipolytica

2014 ◽  
Vol 14 (4) ◽  
pp. 935-946 ◽  
Author(s):  
Anna Czech ◽  
Malwina Merska ◽  
Katarzyna Ognik
Keyword(s):  
Yarrowia Lipolytica ◽  
Hdl Cholesterol ◽  
Defence Mechanisms ◽  
Biochemical Indicators ◽  
Group I ◽  
Lipolytica Strain ◽  
Dietary Nutrient ◽  
Different Content ◽  
Yeast Yarrowia Lipolytica ◽  
Cholesterol Fraction

Abstract The aim of this study was to determine immunological and biochemical blood indicators of turkey hens administered feed mixtures with 3 or 6% of Yarrowia lipolytica strain yeast as a dietary nutrient. The experiment was carried out on 240 turkey hens, aged from 1 to 16 weeks. The hens were randomly assigned to 3 experimental groups of 80 birds. Group I served as a control (K) and did not receive any experimental compounds. The turkey hens from experimental groups (YL3, YL6) were administered dried Yarrowia lipolytica yeast in two doses: 3% (YL3) and 6% (YL6) in feed mixtures. The study showed that the addition of Yarrowia lipolytica yeast in a dose of 3% but mainly in a dose of 6% stimulated the body’s immune defence mechanisms, which was evidenced by the increase in plasma lysozyme, % KF, IF, and reduction of monocyte ratio H/L in turkey hens. The advantage of using Yarrowia lipolytica in the nutrition of turkey hens was also a decrease in the content of blood indicators of lipid peroxidation such as CHOL, TG and LDL-cholesterol fraction, and an increase in the percentage of HDL-cholesterol fraction. The use of yeast component in the feeding of turkey hens affected the health status of birds and contributed to proper (not deviating from the reference values) biochemical indicators of metabolism.

High-efficient production of mono- to few-layer GaSe nanosheets via a novel Na2CO3-promoted ultrasonic exfoliation method

2021 ◽  
Vol 305 ◽  
pp. 130747
Author(s):  
Xiaofei Qi ◽  
Cong Ding ◽  
Mingyang Gao ◽  
Fengcheng Wang ◽  
Yifan Hui ◽  
...  
Keyword(s):  
Efficient Production ◽  
High Efficient

Treatment of Actual Coking Wastewater by Two-Stage EGSB System with Self-Recirculation and Crossed Recirculation

2014 ◽  
Vol 955-959 ◽  
pp. 2374-2377
Author(s):  
Chun Juan Dong ◽  
Tian Tian Zhao ◽  
Qing Ye Pan
Keyword(s):  
Reactor System ◽  
Coking Wastewater ◽  
Efficient Removal ◽  
Two Stage ◽  
N Removal ◽  
Effluent Recirculation ◽  
High Efficient ◽  
Film Reactor ◽  
Removal Efficiencies

To investigate the possibility of simultaneous high efficient removal of COD and NH3-N in the actual coking wastewater through micro-aerobic granular bio-film reactor, two-stage granular bio-film reactor system (EGSBI+ EGSBII) were continuously operated through two effluent recirculation ways for about 5 months: ERW1(24L.h-1 self-recirculation) and ERW2(15 L.h-1 self-recirculation +15 L.h-1 crossed recirculation). Compared with ERW1, through using ERW2, the two-stage EGSB reactor system could attain high COD and NH3-N removal efficiencies (86.7%-96.9% and 48.6%-80.4%) and low effluent NO2- and NO3- concentrations (0-25mg.L-1 and 7-71mg.L-1). Using ERW1, the two-stage EGSB reactor system melt with difficult of NO2- accumulation in the EGSBII (accompanied with low NH3-N removal of only 41.4%). Using ERW2, the difficult of accumulation in the EGSBII was solved and simultaneously high efficient removal of NH3-N and NO2- in the EGSBI was achieved.

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