High-efficient production of mono- to few-layer GaSe nanosheets via a novel Na2CO3-promoted ultrasonic exfoliation method

2021 ◽  
Vol 305 ◽  
pp. 130747
Author(s):  
Xiaofei Qi ◽  
Cong Ding ◽  
Mingyang Gao ◽  
Fengcheng Wang ◽  
Yifan Hui ◽  
...  
2017 ◽  
Vol 173 ◽  
pp. 592-599 ◽  
Author(s):  
Yao-Zu Han ◽  
Chao-Chao Zhou ◽  
Yan-Yan Xu ◽  
Jing-Xin Yao ◽  
Zhe Chi ◽  
...  

Author(s):  
Young-Hyeon An ◽  
Dae Hyun Kim ◽  
Eun Jung Lee ◽  
Dabin Lee ◽  
Mihn Jeong Park ◽  
...  

Recently, the stem cell-derived secretome, which is the set of proteins expressed by stem cells and secreted into the extracellular space, has been demonstrated as a critical contributor for tissue repair. In this study, we have produced two sets of high concentration secretomes from adipose-derived mesenchymal stem cells (ADSCs) that contain bovine serum or free of exogenous molecules. Through proteomic analysis, we elucidated that proteins related to extracellular matrix organization and growth factor-related proteins are highly secreted by ADSCs. Additionally, the application of ADSC secretome to full skin defect showed accelerated wound closure, enhanced angiogenic response, and complete regeneration of epithelial gaps. Furthermore, the ADSC secretome was capable of reducing scar formation. Finally, we show high-dose injection of ADSC secretome via intraperitoneal or transdermal delivery demonstrated no detectable pathological conditions in various tissues/organs, which supports the notion that ADSC secretome can be safely utilized for tissue repair and regeneration.


2018 ◽  
Vol 256 ◽  
pp. 543-547 ◽  
Author(s):  
Sabarathinam Shanmugam ◽  
Chongran Sun ◽  
Xiaoming Zeng ◽  
Yi-Rui Wu

2017 ◽  
Vol 121 ◽  
pp. 185-193 ◽  
Author(s):  
Bo Zhong ◽  
Tianqi Sai ◽  
Long Xia ◽  
Yuanlie Yu ◽  
Guangwu Wen

2021 ◽  
Vol 168 ◽  
pp. 57-63
Author(s):  
Nguyen Le-Phuc ◽  
Tri V. Tran ◽  
Thien T. Phan ◽  
Phuong T. Ngo ◽  
Quan L.M. Ha ◽  
...  

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Zhennan Jiang ◽  
Zhiyong Cui ◽  
Ziwei Zhu ◽  
Yinghang Liu ◽  
Ya-jie Tang ◽  
...  

Abstract Background Succinic acid (SA) is a crucial metabolic intermediate and platform chemical. Development of biobased processes to achieve sustainable SA production has attracted more and more attention in biotechnology industry. Yarrowia lipolytica has a strong tricarboxylic acid cycle and tolerates low pH conditions, thus making it a potential platform for SA production. However, its SA titers in glucose media remain low. Results In this study, we screened mitochondrial carriers and C4-dicarboxylic acid transporters to enhance SA secretion in Y. lipolytica. PGC62-SYF-Mae strain with efficient growth and SA production was constructed by optimizing SA biosynthetic pathways and expressing the transporter SpMae1. In fed-batch fermentation, this strain produced 101.4 g/L SA with a productivity of 0.70 g/L/h and a yield of 0.37 g/g glucose, which is the highest SA titer achieved using yeast, with glucose as the sole carbon resource. Conclusion Our results indicated that transporter engineering is a powerful strategy to achieve the efficient secretion of SA in Y. lipolytica, which will promote the industrial production of bio-based SA.


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