Fabrication of oxidized bacterial cellulose by nitrogen dioxide in chloroform/cyclohexane as a highly loaded drug carrier for sustained release of cisplatin

Oridonin (ORI) is a natural active ingredient with strong anticancer activity. But its clinical use is restricted due to its poor water solubility, short half-life, and low bioavailability. The aim of this study is to utilize the metal organic framework material MOF-5 to load ORI in order to improve its release characteristics and bioavailability. Herein, MOF-5 was synthesized by the solvothermal method and direct addition method, and characterized by Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), Fourier Transform Infrared Spectrometer (FTIR), Thermogravimetric Analysis (TG), Brunauer–Emmett–Teller (BET), and Dynamic Light Scattering (DLS), respectively. MOF-5 prepared by the optimal synthesis method was selected for drug-loading and in vitro release experiments. HepG2 cells were model cells. MTT assay, 4′,6-diamidino-2-phenylindole (DAPI) staining and Annexin V/PI assay were used to detect the biological safety of blank carriers and the anticancer activity of drug-loaded materials. The results showed that nano-MOF-5 prepared by the direct addition method had complete structure, uniform size and good biocompatibility, and was suitable as an ORI carrier. The drug loading of ORI@MOF-5 was 52.86% ± 0.59%. The sustained release effect was reliable, and the cumulative release rate was about 87% in 60 h. ORI@MOF-5 had significant cytotoxicity (IC50:22.99 μg/mL) and apoptosis effect on HepG2 cells. ORI@MOF-5 is hopeful to become a new anticancer sustained release preparation. MOF-5 has significant potential as a drug carrier material.

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Polymeric microspheres for the sustained release of a protein-based drug carrier targeting the PDGFβ-receptor in the fibrotic kidney

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2017.09.072 ◽

2017 ◽

Vol 534 (1-2) ◽

pp. 229-236 ◽

Cited By ~ 14

Author(s):

N. Teekamp ◽

F. Van Dijk ◽

A. Broesder ◽

M. Evers ◽

J. Zuidema ◽

...

Keyword(s):

Sustained Release ◽

Drug Carrier ◽

Polymeric Microspheres

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Coatless alginate pellets as sustained-release drug carrier for inflammatory bowel disease treatment

Carbohydrate Polymers ◽

10.1016/j.carbpol.2016.07.021 ◽

2016 ◽

Vol 152 ◽

pp. 370-381 ◽

Cited By ~ 6

Author(s):

Siti Hajar Md Ramli ◽

Tin Wui Wong ◽

Idanawati Naharudin ◽

Anirbandeep Bose

Keyword(s):

Inflammatory Bowel Disease ◽

Sustained Release ◽

Bowel Disease ◽

Drug Carrier ◽

Disease Treatment ◽

Release Drug ◽

Inflammatory Bowel

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Functional study of early intervention for degenerative retinopathy through hydrogel sustained release system of rhEPO

10.21203/rs.2.16534/v1 ◽

2019 ◽

Author(s):

Zhimeng Zhang ◽

Hehua Dai ◽

Ruyi Li ◽

Yuyu Li ◽

Genlin Li

Keyword(s):

Early Intervention ◽

Sustained Release ◽

Drug Carrier ◽

Retinal Disease ◽

Treated Group ◽

Retinal Function ◽

Functional Study ◽

Release System ◽

Routes Of Administration ◽

Significant Difference

Abstract Background Retinitis pigmentosa (RP) is the most common cause of blindness in retinal disease. Long-lasting ocular administration is an effective therapy to delay the progression of RP. And hydrogel sustained release system may be an available and stable drug carrier in the treatment of RP.Method Hydrogel sustained release system was constructed as a drug carrier of recombinant human erythropoietin (rhEPO). We administered retinal degenerative (rd) mice (Pdeb rd1 / Pdeb rd1 ) via subconjunctival or retrobulbar injection at postnatal 2 weeks (PN-2w), examined the mice and tested the factors of retina at two weeks after injection. Electroretinogram (ERG) was used to examine retinal function at PN-4w, western blot and q-PCR were used to test the expression of Bax, Bcl-2, iNOS and VEGFa of retina.Result Photoreceptor apoptosis were alleviated in all rhEPO administrated groups. The retinal blood supply was improved in injection groups. Compared with placebo and blank control groups, rhEPO treatment could enhance the retinal function and delay the progression of disease. Although there was no significant difference between rhEPO hydrogel and rhEPO treated group, photoreceptor apoptosis in rhEPO hydrogel group was less than that in rhEPO group, and the retinal function was better in rhEPO hydrogel group. Moreover, different routes of administration might have little effect on treatment in this research.Conclusion Early intervention can effectively control the progression of the disease. Anti-apoptosis,neuroprotection and erythropoietin of rhEPO could be useful in the treatment of RP. Hydrogel as a long-lasting drug sustained release system was stable and available, and might become a potential drug carrier in the future.

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Encapsulation of Micro- and Milli-Sized Particles with a Hollow-Type Spherical Bacterial Cellulose Gel via Particle-Preloaded Droplet Cultivation

International Journal of Molecular Sciences ◽

10.3390/ijms20194919 ◽

2019 ◽

Vol 20 (19) ◽

pp. 4919 ◽

Cited By ~ 1

Author(s):

Toru Hoshi ◽

Masashige Suzuki ◽

Mayu Ishikawa ◽

Masahito Endo ◽

Takao Aoyagi

Keyword(s):

Pore Size ◽

Cell Suspension ◽

Bacterial Cellulose ◽

Silicone Oil ◽

Drug Carrier ◽

Immobilized Enzymes ◽

Bioactive Molecules ◽

Interior Space ◽

Alginate Gel ◽

Pass Through

A hollow-type spherical bacterial cellulose (HSBC) gel prepared using conventional methods cannot load particles larger than the pore size of the cellulose nanofiber network of bacterial cellulose (BC) gelatinous membranes. In this study, we prepared a HSBC gel encapsulating target substances larger than the pore size of the BC gelatinous membranes using two encapsulating methods. The first method involved producing the BC gelatinous membrane on the surface of the core that was a spherical alginate gel with a diameter of 2 to 3 mm containing the target substances. With this method, the BC gelatinous membrane was biosynthesized using Gluconacetobacter xylinus at the interface between the cell suspension attached onto the alginate gel and the silicone oil. The second method involved producing the BC gel membrane on the interface between the silicone oil and cell suspension, as well as the spherical alginate gel with a diameter of about 1 mm containing target substances. After the BC gelatinous membrane was biosynthesized, an alginate gel was dissolved in a phosphate buffer to prepare an HSBC gel with the target substances. These encapsulated substances could neither pass through the BC gelatinous membrane of the HSBC gel nor leak from the interior space of the HSBC gel. These results suggest that the HSBC gel had a molecular sieving function. The HSBC gel walls prepared using these methods were observed to be uniform and would be useful for encapsulating bioactive molecules, such as immobilized enzymes in HSBC gel, which is expected to be used as a drug carrier.

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Preparation and characterization of degradable oxidized bacterial cellulose reacted with nitrogen dioxide

Polymer Bulletin ◽

10.1007/s00289-011-0550-8 ◽

2011 ◽

Vol 68 (2) ◽

pp. 415-423 ◽

Cited By ~ 19

Author(s):

Shuai Peng ◽

Yudong Zheng ◽

Jian Wu ◽

Yaxun Wu ◽

Yanxuan Ma ◽

...

Keyword(s):

Nitrogen Dioxide ◽

Bacterial Cellulose

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Easy Preparation of Bacterial Cellulose/Triamcinolone Acetonide Composites and their Inhibition for Fibroblasts

Materials Science Forum ◽

10.4028/www.scientific.net/msf.913.707 ◽

2018 ◽

Vol 913 ◽

pp. 707-713

Author(s):

Miao Ying Jin ◽

Shi Yan Chen ◽

Bao Xiu Wang ◽

Hua Ping Wang

Keyword(s):

Wound Healing ◽

Fourier Transform ◽

Infrared Spectroscopy ◽

Drug Release ◽

Sustained Release ◽

Bacterial Cellulose ◽

Triamcinolone Acetonide ◽

Wound Dressing ◽

Impregnation Method ◽

Relieve Pain

Wound healing is accompanied by keloids, it is important to develop a wound dressing which can inhibit keloid and relieve pain and pruritus in patients. In this paper, bacterial cellulose/triamcinolone acetonide (BC/TA) composites are prepared by easy solution impregnation method, which the structures of BC/TA composites were analyzed by fourier transform infrared spectroscopy (FTIR). The drug release and inhibition of L929 cells were also analyzed. It can be concluded that BC/TA composites have a sustained release effect on TA and can effectively inhibit proliferation of L929 in the range of 0.5-2.0 mg/cm2.

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