Establishment of a novel lectin–antibody ELISA system to determine core-fucosylated haptoglobin

2015 ◽  
Vol 446 ◽  
pp. 30-36 ◽  
Author(s):  
Mayuka Shimomura ◽  
Kotarosumitomo Nakayama ◽  
Kanako Azuma ◽  
Naoko Terao ◽  
Kimihiro Nishino ◽  
...  
2013 ◽  
Vol 417 ◽  
pp. 48-53 ◽  
Author(s):  
Yoshihiro Kamada ◽  
Noriaki Kinoshita ◽  
Yoko Tsuchiya ◽  
Kyoko Kobayashi ◽  
Hironobu Fujii ◽  
...  

Author(s):  
Hitoshi Matsumoto ◽  
Shinichiro Shinzaki ◽  
Megumi Narisada ◽  
Sayuri Kawamoto ◽  
Kana Kuwamoto ◽  
...  

Author(s):  
Nina Reiners ◽  
Carolin Schnurra ◽  
Henning Trawinski ◽  
Judith Kannenberg ◽  
Thomas Hermsdorf ◽  
...  

AbstractSARS CoV-2 antibody assays measure antibodies against the viral nucleoprotein (NP) or spike protein. The study examined if testing of antibodies against both antigens increases the diagnostic sensitivity. Sera (N=98) from infected individuals were tested with ELISAs based on the NP, receptor-binding domain (RBD), or both proteins. The AUROCs were 0.958 (NP), 0.991 (RBD), and 0.992 (NP/RBD). The RBD- and NP/RBD-based ELISAs showed better performance than the NP-based assay. Simultaneous testing for antibodies against NP and RBD increased the number of true and false positives. If maximum diagnostic sensitivity is required, the NP/RBD-based ELISA is preferable. Otherwise, the RBD-based ELISA is sufficient.


Author(s):  
Maria Ganczak ◽  
Katarzyna Topczewska ◽  
Daniel Biesiada ◽  
Marcin Korzeń

To evaluate the prevalence of bloodborne infections (BBIs) and assess the incidence and selected risk factors for sharps injuries (SIs), a cross-sectional serosurvey was performed between December 2018 and October 2019 among 286 paramedics (76.5% males; mean age, 37 years) from 17 randomly selected ambulance stations in the West Pomeranian region of Poland. An ELISA system was used to detect anti-HBc, anti-HCV, and anti-HIV. HBV vaccination uptake was 95.6%; 7.3% (95% CI: 4.6–11.0%) paramedics were anti-HBc positive, and anti-HCV/anti-HIV seropositivity was not reported. Almost one-fourth of paramedics reported having had ≥1 SI during the preceding year (Me = 6.0, range 1–100). Most recent exposures primarily took place during an emergency procedure (76.7%), in an ambulance (45.2%), caused by hollow-bore needles (73.8%), and were not reported (50.0%). Additionally, 52.2% of paramedics reported needle recapping, and 52.6% did not use safety engineered devices (SEDs) at work. Mean knowledge score was low (2.6 ± 1.7); 3.4% had never participated in infection-control (IC) training, and those not trained were more likely to suffer a SI (odds ratio (OR) 4.64; p = 0.03). Due to frequent SIs, of which half are unreported, paramedics remain at risk of acquiring occupational BBIs. SI risk could be reduced by providing training on IC procedures, ensuring better compliance with safe work practices, and supplying more SEDs.


1996 ◽  
Vol 25 (2) ◽  
pp. 245-254 ◽  
Author(s):  
Wojtek P. Michalski ◽  
Denise O'Rourke ◽  
Trevor J. Bagust

2000 ◽  
Vol 66 (9) ◽  
pp. 4149-4151 ◽  
Author(s):  
Wan-Ling Tsai ◽  
Cynthia E. Miller ◽  
Edward R. Richter

ABSTRACT Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IMS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coliO157:H7, at the level of 10−1 CFU/g of sample in either the 25- or 375-g sample size.


Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 358
Author(s):  
Morena Di Tommaso ◽  
Alessia Luciani ◽  
Paolo Emidio Crisi ◽  
Marica Beschi ◽  
Paolo Rosi ◽  
...  

Canine atopic dermatitis (CAD) is a pruritic allergic skin disease associated with IgE-mediated hypersensitivity. IgE is detected using Serum Allergen-Specific IgE test (SAT) in order to identify allergens. The present study aims to identify the environmental allergens in atopic dogs living in Northern Italy using SAT. The screening SAT (sSAT), using a monoclonal antibody cocktail-based ELISA to identify indoor and outdoor allergens, was performed. In all positive samples, an anti-IgE monoclonal antibody ELISA test was performed to extend panel of allergens. Out of 117 selected dogs, 69 were included in the study; 71% were positive and 29% were negative to sSAT. Among the 49 positive sSAT, 53% were positive for both indoor and outdoor, 38.8% only for indoor, and 8.2% only for outdoor allergens. This is the first study on the frequency of allergens involved in CAD in Italy using SAT. IgE hypersensitivity in atopic dogs of Northern Italy is usually associated with indoor allergens, primarily house dust mites. Among the outdoor allergens, an important role was played by Rumex acetosa. Polysensitization also commonly occurs. Therefore, since the numerous factors affect the IgE positivity in CAD, specific panels for geographical areas should be considered and re-evaluated at time intervals.


1987 ◽  
Vol 98 (1) ◽  
pp. 123-127 ◽  
Author(s):  
A.R. Freedman ◽  
G. Galfrè ◽  
E. Gal ◽  
H.J. Ellis ◽  
P.J. Ciclitira

2002 ◽  
Vol 109 (1) ◽  
pp. S329-S329
Author(s):  
Juan A Asturias ◽  
Carmen Arilla ◽  
Ignacio Ibarrola ◽  
Elena Eraso ◽  
Jaime Algorta ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document