Reevaluation of a lectin antibody ELISA kit for measuring fucosylated haptoglobin in various conditions

AbstractFucosylation is one of the most important glycosylation events involved in cancer and inflammation. We previously developed a lectin antibody ELISA kit to measure fucosylated haptoglobin (Fuc-Hpt), which we identified as a novel cancer biomarker. In this study, we investigated Fuc-Hpt as a biomarker in chronic liver diseases, especially in hepatocellular carcinoma (HCC).We measured serum Fuc-Hpt levels using our ELISA kit in 318 patients with chronic liver diseases, including 145 chronic hepatitis (CH) patients, 81 liver cirrhosis (LC) patients, and 92 HCC patients. During a long-term follow-up period of 7 years (1996–2003), Fuc-Hpt levels were measured at three different time points in 19 HCC patients. Serum Fuc-Hpt levels were also examined with a short-term follow-up period of 3 years (2009–2012) in 13 HCC patients.Fuc-Hpt levels increased with liver disease progression. Patients with LC and HCC showed significantly increased Fuc-Hpt levels in comparison to CH patients or healthy volunteers. Fuc-Hpt levels tended to be higher in HCC patients than in LC patients. Fuc-Hpt was better than α-fetoprotein (AFP) and AFP-L3 for predicting HCC [diagnosed by computed tomography (CT) or ultrasound] in LC patients with long-term follow-up. More than 80% of LC patients with long-term follow-up showed increased Fuc-Hpt during hepatocarcinogenesis, and 38% of early-stage HCC patients with short-term follow-up showed a gradual increase in Fuc-Hpt before imaging diagnosis.These results suggest that Fuc-Hpt is a novel and potentially useful biomarker for predicting liver disease progression and HCC development.

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Detection of Hepatitis B Genotypes in HBsAg & HBeAg Carriers

Journal of Biomedical and Pharmaceutical Research ◽

10.32553/jbpr.v7i5.556 ◽

2018 ◽

Vol 7 (5) ◽

Author(s):

Salman Khan ◽

Molly Madan

Keyword(s):

Chronic Hepatitis ◽

Viral Load ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Chronic Hepatitis B ◽

Central Research ◽

Elisa Kit ◽

B Virus ◽

Negative Chronic Hepatitis ◽

Genotype A

Objective:- Hepatitis B is noteworthy medical issues that may include the late continuation of liver cirrhosis and hepatocellular carcinoma. The present study aimed for the detection and diffrentiation of Hepatitis B virus HBsAg inactive non-replicative carriers, HBeAg-positive inactive replicative carriers, active carriers & HBeAg-negative chronic hepatitis B by Real Time PCR and their genotyping Methods: This research conducted on 245 positive for HBsAg, 118 (48.16 %) were male and 127 (51.84%) were female patients, which was performed in central research station labortory of Microbiology at netaji subhash Chandra Bose subharti Medical College and Hospital, Meerut Between march 2016 to November 2017 The sera were separated and screened for HBsAg by ELISA kit. Positive samples for HBsAg were tested for HBeAg ELISA kit and DNA Viral load then sequenced for genotying Results:. Of the 245 HBsAg Positive case 55 (1.12%) were HBeAg positive. In 16 PCR positive and HBV genotyping, In HBsAg inactive Non-Replicative 37.5% (n=6) genotype-B and 6.25% (n=1) genotype-A, In HBeAg inactive Replicative 12.5% (n=2) genotype-B and 12.5% (n=2) genotype-A and In HBeAg Active Chronic Hepatitis B 18.75% (n=3) genotype-B and 12.5% (n=2) genotype-A were detected Conclusions: Management strategy, using HBsAg, HBeAg and HBV DNA viral load, seems adequate for the confirmation and diffrentiation of Hepatitis B virus inactive, active carriers & HBeAg-negative chronic hepatitis B patients and genotype B was more prevalent in comparission to genotype A. Distribution of carriers & genotypes, help physicians to prescribe proper antiviral/interferon therapy according to current genotyping pattern in this region Keywords: Hepatitis B virus, Carrier State, HBsAg, HBeAg, RT-PCR

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A Cross Sectional Survey of Chicken Astroviruses Antibody in Broiler and Sonali (Cross-Bred) Chickens in Selected Areas in Bangladesh

Macedonian Veterinary Review ◽

10.2478/macvetrev-2020-0016 ◽

2020 ◽

Vol 43 (1) ◽

pp. 75-80

Author(s):

Md Zulfekar Ali ◽

Mohammad Moktader Moula ◽

Zafar Ahmed Bhuiyan ◽

Muhammad Tariq Javed

Keyword(s):

Age Groups ◽

Winter Season ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Blood Samples ◽

Elisa Kit ◽

Published Report ◽

Poultry Birds ◽

Wide Range ◽

Group B

AbstractChicken astroviruses (CAstV) are enteric viruses of poultry causing gastroenteritis, malabsorption, gout and white chick disease commonly known as runting-stunting syndrome (RSS). It can affect the wide range of poultry birds, especially chicken, turkey and duck worldwide. To our best knowledge there is no published report on presence of antibodies against CAstV in Bangladesh. Therefore, the study aimed to detect the presence of CAstV antibodies in broilers and sonali chickens (a cross-bread) in Bangladesh through a cross-sectional survey. A total of 454 blood samples from 66 flocks of broiler (n=343) and sonali chickens (n=111) of different ages were obtained during 2017 from four districts. The birds were healthy but were not vaccinated against CAstV. The samples were tested for specific antibodies against CAstV Group B by using commercially available ELISA kit. Overall, 16.74% (76/454) samples and 34.84% (23/66) flocks were positive for CAstV antibodies. The seroprevalence of CAstV was significantly (p=0.001) higher in sonali chickens (36.96%) than broiler (10.20%), while it was significantly higher (p=0.001) in birds of Bogura district (36.94%) than the other three districts. Regarding the age groups, seroprevalence was insignificantly (p=0.192) higher in sonali chicken before laying age (45%) than during laying age (27.45%). Regarding the seasons, CAstV infection was prevalent significantly (p=0.001) higher in winter season. Thus, the present study indicated the presence of CAstV in poultry in Bangladesh, so further studies are required to find out the magnitude of the problem in the country.

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Can Osteopontin Be Considered a Biomarker for Endometriosis?

Revista de Chimie ◽

10.37358/rc.17.9.5840 ◽

2017 ◽

Vol 68 (9) ◽

pp. 2132-2134

Author(s):

Daniela Roxana Albu (Matasariu) ◽

Elena Mihalceanu ◽

Alina Pangal ◽

Carmen Vulpoi ◽

Mircea Onofriescu ◽

...

Keyword(s):

Serum Level ◽

Immunosorbent Assay ◽

Pelvic Pain ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Multifactorial Disease ◽

Elisa Kit ◽

Healthy Women ◽

Progesterone Treatment ◽

Large Dispersion

Endometriosis is a multifactorial disease that is manifested by infertility and pelvic pain. The purpose of the study was to evaluate the effect of progesterone treatment on the serum level of osteopontin, a multipotent cytokine, in patients with endometriosis. The study was prospective and we evaluated osteopontin levels that were measured in the serum of 40 patients with endometriosis and 12 healthy women using a standardized Enzyme-Linked Immunosorbent Assay (ELISA) kit. Osteopontin seric levels were lower in endometriosis patients and increased after progesterone treatment. Because of the large dispersion of data even in the control group, we find the association between osteopontin and endometriosis questionable.

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Nontuberculous Mycobacterium Infections in Rheumatoid Arthritis Patients: The Serodiagnosis of Pulmonary Disease due to Mycobacterium avium Complex with an Enzyme Immunoassay Kit Detecting Glycopeptidolipid Core Antigen (Capilia MAC Antibody ELISA)®)

Current Rheumatology Reviews ◽

10.2174/1573397111309020007 ◽

2013 ◽

Vol 9 (2) ◽

pp. 119-130 ◽

Cited By ~ 1

Author(s):

Maiko Watanabe ◽

Shogo Banno

Keyword(s):

Rheumatoid Arthritis ◽

Enzyme Immunoassay ◽

Pulmonary Disease ◽

Mycobacterium Avium ◽

Mycobacterium Avium Complex ◽

Nontuberculous Mycobacterium ◽

Core Antigen ◽

Mycobacterium Infections ◽

Antibody Elisa

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Performance of a SARS CoV-2 antibody ELISA based on simultaneous measurement of antibodies against the viral nucleoprotein and receptor-binding domain

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-021-04284-5 ◽

2021 ◽

Author(s):

Nina Reiners ◽

Carolin Schnurra ◽

Henning Trawinski ◽

Judith Kannenberg ◽

Thomas Hermsdorf ◽

...

Keyword(s):

Receptor Binding ◽

Simultaneous Measurement ◽

False Positives ◽

Binding Domain ◽

Diagnostic Sensitivity ◽

Spike Protein ◽

Receptor Binding Domain ◽

Simultaneous Testing ◽

Antibody Assays ◽

Antibody Elisa

AbstractSARS CoV-2 antibody assays measure antibodies against the viral nucleoprotein (NP) or spike protein. The study examined if testing of antibodies against both antigens increases the diagnostic sensitivity. Sera (N=98) from infected individuals were tested with ELISAs based on the NP, receptor-binding domain (RBD), or both proteins. The AUROCs were 0.958 (NP), 0.991 (RBD), and 0.992 (NP/RBD). The RBD- and NP/RBD-based ELISAs showed better performance than the NP-based assay. Simultaneous testing for antibodies against NP and RBD increased the number of true and false positives. If maximum diagnostic sensitivity is required, the NP/RBD-based ELISA is preferable. Otherwise, the RBD-based ELISA is sufficient.

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Automated Western immunoblotting detection of anti-SARS-CoV-2 serum antibodies

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-021-04203-8 ◽

2021 ◽

Author(s):

Sophie Edouard ◽

Rita Jaafar ◽

Nicolas Orain ◽

Philippe Parola ◽

Philippe Colson ◽

...

Keyword(s):

Negative Control ◽

Cross Reactivity ◽

Spike Protein ◽

Rt Pcr ◽

Substantial Agreement ◽

Western Immunoblotting ◽

Elisa Kit ◽

Line Test ◽

Serologic Assay ◽

Control Samples

AbstractELISA and chemiluminescence serological assays for COVID-19 are currently incorporating only one or two SARS-CoV-2 antigens. We developed an automated Western immunoblotting as a complementary serologic assay for COVID-19. The JessTM Simple Western system, an automated capillary-based assay, was used, incorporating an inactivated SARS-CoV-2 lineage 20a strain as the source of antigen, and total immunoglobulins (IgG, IgM, IgA) detection. In total, 602 sera were tested including 223 from RT-PCR-confirmed COVID-19 patients, 76 from patients diagnosed with seasonal HCoVs and 303 from coronavirus-negative control sera. We also compared this assay with the EUROIMMUN® SARS-CoV-2 IgG ELISA kit. Among 223 sera obtained from RT-PCR-confirmed COVID-19 patients, 180/223 (81%) exhibited reactivity against the nucleocapsid and 70/223 (31%) against the spike protein. Nucleocapsid reactivity was further detected in 9/76 (14%) samples collected from patients diagnosed with seasonal HCoVs and in 15/303 (5%) coronavirus-negative control samples. In the subset of sera collected more than 2 weeks after the onset of symptoms, the sensitivity was 94% and the specificity 93%, the latter value probably reflecting cross-reactivity of SARS-CoV-2 with other coronaviruses. The automated Western immunoblotting presented a substantial agreement (90%) with the compared ELISA (Cohen’s Kappa=0.64). Automated Western immunoblotting may be used as a second line test to monitor exposure of people to HCoVs including SARS-CoV-2.

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