Optimizing lime pretreatment of rice straw for biolipid production using oleaginous microorganisms

Chemosphere ◽  
2021 ◽  
Vol 269 ◽  
pp. 129390
Author(s):  
Hoa Thai Doan ◽  
Phuong Thi Minh Nguyen ◽  
Thuy Thanh Tran ◽  
Thoa Kim Nguyen ◽  
Man Dinh Tran ◽  
...  
2014 ◽  
Vol 71 ◽  
pp. 106-112 ◽  
Author(s):  
Yue-gan Liang ◽  
Beijiu Cheng ◽  
You-bin Si ◽  
De-ju Cao ◽  
Er Nie ◽  
...  

2016 ◽  
Vol 36 (03) ◽  
pp. 253
Author(s):  
Dhina Aprilia Nurani Widyahapsari ◽  
Retno Indrati ◽  
Sigit Setyabudi ◽  
Sardjono Sardjono

L-lactic acid can be used as a precursor of polylactic acid (PLA). PLA is a biodegradable biomaterial commonly used for biodegradable plastics. Lactic acid can be produced from lignocelluloses materials such as rice straw. Rice straw is composed of cellulose and hemicellulose that can be hydrolyzed to fermentable sugar by cellulolytic and hemicellulolytic enzymes then converted to L-lactic acid by Rhizopus oryzae. As most cellulose and hemicellulose present in lignocellulose biomass are not readily accessible for these enzyme, pretreatment is required to alter the structure of lignocellulose substrates. This research aimed to investigate the effect of lime pretreatment on rice straw bioconversion to L-lactic acid by Rhizopus oryzae AT3. Rice straw was pretreated with lime (Ca(OH)2) at 85 °C for 16 hours. Unpretreated and pretreated rice straw were hydrolyzed using crude enzyme that produced by Trichoderma reesei Pk1J2. Enzyme production was carried out by solid state fermentation using rice straw and rice brand as substrate. Enzymatic hydrolysis was carried out in flasks. Each flask was added with unpretreated or pretreated rice straw, buffer citrate solution and crude enzyme then hydrolyzed for 0-96 hours. Hydrolysate was fermented by Rhizopus oryzae AT3 for 0-6 days by using adsorbed carrier solid-state fermentation method with polyurethane foam as inert support material. Lime pretreatment at 85 °C for 16 hour led to significant solubilisation of lignin and hemicellulose. It involved lignocellulose structure modified that enhance enzymatic hydrolysis and resulted higher reducing sugars than unpretreated rice straw. The high reducing sugars was not related to high lactic acid yields. Fermentation of pretreated rice straw hydrolysate by Rhizopus oryzae AT3 did not only produce L-lactic acid but also other compound. On the other hand, fermentation of unpretreated rice straw hydrolysate only produced L-lactic acid. ABSTRAKPolimerisasi asam laktat menjadi polylactic acid untuk menghasilkan biodegradable plastic membutuhkan asam laktat dengan isomer spesifik. Rhizopus oryzae adalah mikroorganisme yang spesifik menghasilkan L-asam laktat. Selain itu Rhizopus oryzae dapat menggunakan limbah pertanian seperti jerami padi sebagai substrat. Komponen utama jerami padi merupakan lignoselulosa yang dapat dihidrolisa secara enzimatis menjadi komponen gula sederhana penyusunnya dan selanjutnya dapat dikonversi menjadi L-asam laktat oleh Rhizopus oryzae. Namun struktur lignoselulosa sangat kompak dan rapat, sulit untuk dihidrolisa secara enzimatis sehingga diperlukan adanya perlakuan pendahuluan untuk merombak struktur lignoselulosa agar mudah dihidrolisa. Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan pendahuluan menggunakan kalsium hidroksida (Ca(OH)2) terhadap biokonversi jerami padi menjadi L-asam laktat oleh Rhizopus oryzae AT3. Perlakuan pendahuluan pada jerami padi dilakukan menggunakan (Ca(OH)2) disertai pemanasan suhu 85 °C selama 16 jam. Jerami padi dengan dan tanpa perlakuan pendahuluan dihidrolisa secara enzimatis menggunakan crude enzyme yang diproduksi oleh Trichoderma reesei Pk1J2. Produksi crude enzyme dilakukan dengan fermentasi substrat padat dengan campuran jerami padi dan dedak sebagai substrat. Hidrolisat jerami padi dengan dan tanpa perlakuan pendahuluan selanjutnya difermentasi oleh Rhizopus oryzae AT3 menggunakan metode adsorbed carrier solid state fermentation dengan polyurethane foam (PUF) sebagai bahan pendukung. Perlakuan pendahuluan menggunakan Ca(OH2) disertai pemanasan suhu 85 °C selama 16 jam dapat merubah komposisi lignoselulosa jerami padi yaitu dengan melarutkan lignin dan hemiselulosa. Perubahan komposisi lignoselulosa memudahkan kerja crude enzyme dalam menghidrolisa jerami padi sehingga menghasilkan gula reduksi lebih tinggi dibandingkan jerami padi tanpa perlakuan pendahuluan. Tingginya gula reduksi tidak serta merta meningkatkan yield L-asam laktat yang dihasilkan. Fermentasi hidrolisat jerami padi dengan perlakuan pendahuluan oleh Rhizopus oryzae AT3 menghasilkan yield L-asam laktat lebih rendah dibandingkan hidrolisat jerami padi tanpa perlakuan pendahuluan. Namun pada jerami padi dengan perlakuan pendahuluan dihasilkan senyawa lain selain asam laktat.Kata Kunci: Adsorbed carrier solid state fermentation; L-asam laktat; perlakuan pendahuluan; Rhizopus oryzae AT3; jerami padi


2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Zilin Song ◽  
Gaihe Yang ◽  
Xinhui Han ◽  
Yongzhong Feng ◽  
Guangxin Ren

The lime pretreatment process for rice straw was optimized to enhance the biodegradation performance and increase biogas yield. The optimization was implemented using response surface methodology (RSM) and Box-Behnken experimental design. The effects of biodegradation, as well as the interactive effects of Ca(OH)2concentration, pretreatment time, and inoculum amount on biogas improvement, were investigated. Rice straw compounds, such as lignin, cellulose, and hemicellulose, were significantly degraded with increasing Ca(OH)2concentration. The optimal conditions for the use of pretreated rice straw in anaerobic digestion were 9.81% Ca(OH)2(w/w TS), 5.89 d treatment time, and 45.12% inoculum content, which resulted in a methane yield of 225.3 mL/g VS. A determination coefficient (R2) of 96% was obtained, indicating that the model used to predict the anabolic digestion process shows a favorable fit with the experimental parameters.


2010 ◽  
Vol 101 (17) ◽  
pp. 6805-6811 ◽  
Author(s):  
Jeung-yil Park ◽  
Riki Shiroma ◽  
Muhammad Imran Al-Haq ◽  
Ying Zhang ◽  
Masakazu Ike ◽  
...  

2011 ◽  
Vol 102 (3) ◽  
pp. 2943-2949 ◽  
Author(s):  
Riki Shiroma ◽  
Jeung-yil Park ◽  
Muhammad Imran Al-Haq ◽  
Mitsuhiro Arakane ◽  
Masakazu Ike ◽  
...  

1977 ◽  
Vol 27 (1) ◽  
pp. 93-98
Author(s):  
Naim A. Fadl ◽  
Magdi Z. Sefain ◽  
Mohomed Rakha
Keyword(s):  

2018 ◽  
Vol 9 (05) ◽  
pp. 20218-20223
Author(s):  
I Ketut Arnawa ◽  
I Wayan Runa ◽  
I Made Sukerta ◽  
Putu Sri Astuti ◽  
Dan Luh Kadek Budi Martini

The main purpose for the application of science and technology for livestock group of cow, is to form a group of skilled and professional livestock, as vaccinators, can take advantage of Azadirachta indica leaves a plant insecticide, apply a good cage sanitation, can produce organic fertilizer from feces of cow, can treat hay as feed to good nutritional value. The method used is education and training. Activity results obtained, that 10 percent of members of the group have managed to become vaccinators cadres, able to implement good sanitation stables, feces of cow (Stomoxys calsitrans) can process technology with EM4 into organic fertilizer and can process the rice straw with urea spraying method, to produce feed hay to the value of good nutrition for growing cattle.


Author(s):  
Phan Vũ Hải ◽  
Hồ Trung Thông ◽  
Đàm Văn Tiện

This study was undertaken to find ways of reducing the time taken by goats tobegin to eat an edible feed that they have not previously encountered. Experiment 1demonstrated that the time taken for goats (7-8 months old) to ingest an unfamiliar feed(rice straw) was shorter (4 days) when it was first offered to them in the presence offamiliar positive cues (the odor or flavor of juices extracted from previously eaten,nutritionally beneficial grasses), than if it was offered in the absence of such cues (10 days).In contrast, when the feed was offered in the presence of the odor of parasitised goat feces,the time to first ingestion was extended to 20 days. Experiment 2 showed that when sixmonthold goats were exposed to feeds they had not experienced previously (rice straw orrice bran) they did not ingest these feeds in less than 7 days. However, they commencedingesting these feeds immediately if they had been exposed to them, prior to weaning, inthe presence of their mother or another adult goat. Application of the principles of feedingbehavior, as illustrated by the present studies, to goats in Vietnam may improve theirproduction, especially when diets are changed frequently and include both familiar andunfamiliar materials.Keywords: Behavior; Diet selection; Flavor; Neophobia; Social facilitation; Goat.


2019 ◽  
Vol 3 (2) ◽  
pp. 172
Author(s):  
Sri - Purwanti ◽  
Syamsuddin Nompo ◽  
Laily Agustina ◽  
Muh. Nur Hidayat

The activities conducted in Maros regency Tanralili district Purnakarya village in chickens and beef cattle groups Cedde Jaya. The main source of livestock feed cows during the dry season are dry forage, rice straw, corn straw and hay crops of nuts which happened to be planted at the time of drought. Besides, leaf  Gamal is also a main feed in dry  season. Number of cattle per family ownership only 2-3  head extensively maintained. Range chicken as well as extensive maintenance, ownership on average 2-5 chicken each household group.  To increase the productivity of cattle, the team provides counseling, coaching, training and mentoring as well as providing some main of facilities such as cattle shed, biogas digester, planting banana. Results are expected in the presence of  activities is the avaibility of green forage mainly during the dry season, so that the cattle population will increase, the biogas as a fuel  alternative to kerosin and LPG gas, liquid and solid fertilizer from biogas by-product process, the increasing population of chicken qualified (meat and eggs as well as the day old chick).  Besides the problem of production is also done on the farm management. The activities is expected that will increase the incomes of farmers further improve the welfare of Maros regency. For the sustainability of the program it is necessary supervision and guidance are routinely performed by the related department along with the college.


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