RT-CaCCO process: An improved CaCCO process for rice straw by its incorporation with a step of lime pretreatment at room temperature

The purpose of this research was to analyze the biogas production from co-digestion of Salvinia molesta and rice straw. Ratio of Salvinia molesta and rice straw was 5:0, 4:1, 3:2. Lab-scale-batch digesters (600 mL) were operated at room temperature (30 oC) and pressure of 1 atm. Total basis of Salvinia molesta and rice straw was 10 gr, water was added with ratio of organic matter:water = 1:7 (w/w), rumen fluid was added as inoculum, initial pH was adjusted to be 7. Fermentation process was conducted for 30 days. The results showed that total biogas volume for ratio of 5:0, 4:1, 3:2 was 6.300.00; 32.7618.32; 107.5418.51 mL/g VS respectively. The pH of substrate was changing from 7.00 to 6.770.19; 6.600.14; 6.730.09 for all variables respectively.

Download Full-text

Effect of Cellulase Enzymes on Degradation Characteristics of Ensiled Rice Straw

Proceedings of the British Society of Animal Production (1972) ◽

10.1017/s0308229600017219 ◽

1988 ◽

Vol 1988 ◽

pp. 83-83

Author(s):

Y. Nakashima ◽

E.R. Ørskov ◽

K. Ambo ◽

Y. Takase

Keyword(s):

Rice Straw ◽

Dry Matter ◽

Room Temperature ◽

Degradation Rate ◽

Enzyme Preparation ◽

Lower Content ◽

Cellulase Enzymes ◽

Cellulase Enzyme ◽

Physical Form ◽

Degradation Rate Constant

AbstractRice straw was ensiled in laboratory containers of 11 capacity. Three concentrations of moisture (50, 60 or 70%) 3 concentrations of a commercial cellulase enzyme preparation (0, 5 or 10g/kg dry matter (DM)) and 3 types of straw processing (2 cm, 5 mm or 2 mm length) were used. The preparations were stored at room temperature (approximately 20°C) for 30 days. The straw treated with cellulase had a lower final pH (5.21, 4.87, 4.82; P<0.05), higher concentrations of lactic acid (198, 383, 367 mg/100g; P<0.05), a lower content of neutral detergent fibre (689, 630, 620 g/kg DM; P<0.05) and a higher solubility, measured as washing losses from nylon bags (152, 196, 212 g/kg DM; P<0.05) for the 0, 5 and 10 g/kg cellulase treatments respectively. The samples were subsequently incubated in nylon bags in the rumen of 3 sheep for 8, 16, 24, 48 and 72 h to estimate degradation rate and potential degradability using the expression p+a+b(1-e-ct) where p is degradability at time t and a, b and c are constants. While there was no effect of moisture content or physical form of the straw, the degradation rate constant (c) was significantly increased (P<0.05) by the addition of cellulase. The maximum potential (a+b) however was unchanged. The values for c were 0.0496, 0.0677 and 0.0847/h-1 and the values for (a+b) were 624, 621 and 628 g/kg for the 0, 5 and 10 g/kg cellulase enzyme additions respectively. It is concluded that the use of cellulase enzymes can assist in the preservation of wet straw and can result in improved degradation characteristics.

Download Full-text

Physicochemical changes of rice straw after lime pretreatment and mesophilic dry digestion

Biomass and Bioenergy ◽

10.1016/j.biombioe.2014.10.020 ◽

2014 ◽

Vol 71 ◽

pp. 106-112 ◽

Cited By ~ 11

Author(s):

Yue-gan Liang ◽

Beijiu Cheng ◽

You-bin Si ◽

De-ju Cao ◽

Er Nie ◽

...

Keyword(s):

Rice Straw ◽

Physicochemical Changes ◽

Lime Pretreatment ◽

Dry Digestion

Download Full-text

Evaluasi Perlakuan Pendahuluan Menggunakan Kalsium Hidroksida untuk Biokonversi Jerami Padi Menjadi L-Asam Laktat oleh Rhizopus oryzae AT3 (Evaluation of Lime Pretreatment for Bioconversion of Rice Straw to L-Lactic Acid by Rhizopus Oryzae AT3)

Jurnal Agritech ◽

10.22146/agritech.16587 ◽

2016 ◽

Vol 36 (03) ◽

pp. 253

Author(s):

Dhina Aprilia Nurani Widyahapsari ◽

Retno Indrati ◽

Sigit Setyabudi ◽

Sardjono Sardjono

Keyword(s):

Lactic Acid ◽

Solid State ◽

Enzymatic Hydrolysis ◽

Rice Straw ◽

Solid State Fermentation ◽

Reducing Sugars ◽

Rhizopus Oryzae ◽

Crude Enzyme ◽

Lime Pretreatment ◽

Rice Straw Hydrolysate

L-lactic acid can be used as a precursor of polylactic acid (PLA). PLA is a biodegradable biomaterial commonly used for biodegradable plastics. Lactic acid can be produced from lignocelluloses materials such as rice straw. Rice straw is composed of cellulose and hemicellulose that can be hydrolyzed to fermentable sugar by cellulolytic and hemicellulolytic enzymes then converted to L-lactic acid by Rhizopus oryzae. As most cellulose and hemicellulose present in lignocellulose biomass are not readily accessible for these enzyme, pretreatment is required to alter the structure of lignocellulose substrates. This research aimed to investigate the effect of lime pretreatment on rice straw bioconversion to L-lactic acid by Rhizopus oryzae AT3. Rice straw was pretreated with lime (Ca(OH)2) at 85 °C for 16 hours. Unpretreated and pretreated rice straw were hydrolyzed using crude enzyme that produced by Trichoderma reesei Pk1J2. Enzyme production was carried out by solid state fermentation using rice straw and rice brand as substrate. Enzymatic hydrolysis was carried out in flasks. Each flask was added with unpretreated or pretreated rice straw, buffer citrate solution and crude enzyme then hydrolyzed for 0-96 hours. Hydrolysate was fermented by Rhizopus oryzae AT3 for 0-6 days by using adsorbed carrier solid-state fermentation method with polyurethane foam as inert support material. Lime pretreatment at 85 °C for 16 hour led to significant solubilisation of lignin and hemicellulose. It involved lignocellulose structure modified that enhance enzymatic hydrolysis and resulted higher reducing sugars than unpretreated rice straw. The high reducing sugars was not related to high lactic acid yields. Fermentation of pretreated rice straw hydrolysate by Rhizopus oryzae AT3 did not only produce L-lactic acid but also other compound. On the other hand, fermentation of unpretreated rice straw hydrolysate only produced L-lactic acid. ABSTRAKPolimerisasi asam laktat menjadi polylactic acid untuk menghasilkan biodegradable plastic membutuhkan asam laktat dengan isomer spesifik. Rhizopus oryzae adalah mikroorganisme yang spesifik menghasilkan L-asam laktat. Selain itu Rhizopus oryzae dapat menggunakan limbah pertanian seperti jerami padi sebagai substrat. Komponen utama jerami padi merupakan lignoselulosa yang dapat dihidrolisa secara enzimatis menjadi komponen gula sederhana penyusunnya dan selanjutnya dapat dikonversi menjadi L-asam laktat oleh Rhizopus oryzae. Namun struktur lignoselulosa sangat kompak dan rapat, sulit untuk dihidrolisa secara enzimatis sehingga diperlukan adanya perlakuan pendahuluan untuk merombak struktur lignoselulosa agar mudah dihidrolisa. Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan pendahuluan menggunakan kalsium hidroksida (Ca(OH)2) terhadap biokonversi jerami padi menjadi L-asam laktat oleh Rhizopus oryzae AT3. Perlakuan pendahuluan pada jerami padi dilakukan menggunakan (Ca(OH)2) disertai pemanasan suhu 85 °C selama 16 jam. Jerami padi dengan dan tanpa perlakuan pendahuluan dihidrolisa secara enzimatis menggunakan crude enzyme yang diproduksi oleh Trichoderma reesei Pk1J2. Produksi crude enzyme dilakukan dengan fermentasi substrat padat dengan campuran jerami padi dan dedak sebagai substrat. Hidrolisat jerami padi dengan dan tanpa perlakuan pendahuluan selanjutnya difermentasi oleh Rhizopus oryzae AT3 menggunakan metode adsorbed carrier solid state fermentation dengan polyurethane foam (PUF) sebagai bahan pendukung. Perlakuan pendahuluan menggunakan Ca(OH2) disertai pemanasan suhu 85 °C selama 16 jam dapat merubah komposisi lignoselulosa jerami padi yaitu dengan melarutkan lignin dan hemiselulosa. Perubahan komposisi lignoselulosa memudahkan kerja crude enzyme dalam menghidrolisa jerami padi sehingga menghasilkan gula reduksi lebih tinggi dibandingkan jerami padi tanpa perlakuan pendahuluan. Tingginya gula reduksi tidak serta merta meningkatkan yield L-asam laktat yang dihasilkan. Fermentasi hidrolisat jerami padi dengan perlakuan pendahuluan oleh Rhizopus oryzae AT3 menghasilkan yield L-asam laktat lebih rendah dibandingkan hidrolisat jerami padi tanpa perlakuan pendahuluan. Namun pada jerami padi dengan perlakuan pendahuluan dihasilkan senyawa lain selain asam laktat.Kata Kunci: Adsorbed carrier solid state fermentation; L-asam laktat; perlakuan pendahuluan; Rhizopus oryzae AT3; jerami padi

Download Full-text

Screening of Organic Substrates for Solid-State Fermentation, Viability and Bioefficacy of Trichoderma harzianum AS12-2, a Biocontrol Strain Against Rice Sheath Blight Disease

Agronomy ◽

10.3390/agronomy10091258 ◽

2020 ◽

Vol 10 (9) ◽

pp. 1258 ◽

Cited By ~ 1

Author(s):

Shahram Naeimi ◽

Vahid Khosravi ◽

András Varga ◽

Csaba Vágvölgyi ◽

László Kredics

Keyword(s):

Rice Straw ◽

Rice Husk ◽

Room Temperature ◽

Organic Substrates ◽

Sheath Blight Disease ◽

Beet Pulp ◽

Broom Sorghum ◽

Blight Disease ◽

One Year ◽

Sorghum Grain

The present study was undertaken to find the most suitable organic substrates for the biomass production, viability and efficacy of the biocontrol strain Trichoderma harzianum AS12-2 in the solid-state fermentation system. In total, 13 inexpensive, locally available substrates (agricultural wastes or by-products) were inoculated with the antagonist, and following one month of incubation at room temperature, all colonized substrates were air dried and ground to powder. The shelf life and viability of the Trichoderma strain were assessed as colony-forming units per gram (CFUs g−1) of each substrate on a monthly basis for up to one year at room temperature (25 ± 2 °C) and in the refrigerator (4 °C). In order to find out the effect of the substrate on the bioefficacy of T. harzianum AS12-2, the biocontrol potential of the formulations was evaluated against rice sheath blight disease caused by Rhizoctonia solani. The results showed that the fungus colonized more or less all substrates after one month, although the degree of colonization and conidiation was different among the substrates, being especially high in broom sorghum grain, rice husk, rice straw, rice bran and sugar beet pulp. Analysis of variance (ANOVA) of the population in the substrates in “Month 0” showed that the effect of treatment was significant, and the means were significantly different. The maximum population was recorded for broom sorghum grain and rice straw (6.4 × 1010 and 5.3 × 1010 CFUs g−1, respectively). The population declined in all substrates after one year of incubation at room temperature. This decline was relatively smaller in broom sorghum grain, rice straw and rice husk. On the other hand, the population in the same substrate incubated in the refrigerator was decreased in a mild slope, and the final population was high. In addition, the results of greenhouse assay showed that all bioformulations were effective in controlling the disease, and there were no significant differences among the substrates. According to the results of this study, broom sorghum grain, rice husk, rice straw, sugar beet pulp and cow dung could be recommended as suitable fermentation media for the industrial-scale production of T. harzianum strains.

Download Full-text

Optimization of the Alkaline Pretreatment of Rice Straw for Enhanced Methane Yield

BioMed Research International ◽

10.1155/2013/968692 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 10

Author(s):

Zilin Song ◽

Gaihe Yang ◽

Xinhui Han ◽

Yongzhong Feng ◽

Guangxin Ren

Keyword(s):

Rice Straw ◽

Treatment Time ◽

Interactive Effects ◽

Methane Yield ◽

Optimal Conditions ◽

Biogas Yield ◽

Digestion Process ◽

Lime Pretreatment ◽

Experimental Parameters ◽

Pretreatment Time

The lime pretreatment process for rice straw was optimized to enhance the biodegradation performance and increase biogas yield. The optimization was implemented using response surface methodology (RSM) and Box-Behnken experimental design. The effects of biodegradation, as well as the interactive effects of Ca(OH)2concentration, pretreatment time, and inoculum amount on biogas improvement, were investigated. Rice straw compounds, such as lignin, cellulose, and hemicellulose, were significantly degraded with increasing Ca(OH)2concentration. The optimal conditions for the use of pretreated rice straw in anaerobic digestion were 9.81% Ca(OH)2(w/w TS), 5.89 d treatment time, and 45.12% inoculum content, which resulted in a methane yield of 225.3 mL/g VS. A determination coefficient (R2) of 96% was obtained, indicating that the model used to predict the anabolic digestion process shows a favorable fit with the experimental parameters.

Download Full-text

A novel lime pretreatment for subsequent bioethanol production from rice straw – Calcium capturing by carbonation (CaCCO) process

Bioresource Technology ◽

10.1016/j.biortech.2010.03.098 ◽

2010 ◽

Vol 101 (17) ◽

pp. 6805-6811 ◽

Cited By ~ 79

Author(s):

Jeung-yil Park ◽

Riki Shiroma ◽

Muhammad Imran Al-Haq ◽

Ying Zhang ◽

Masakazu Ike ◽

...

Keyword(s):

Rice Straw ◽

Bioethanol Production ◽

Lime Pretreatment

Download Full-text

Optimizing lime pretreatment of rice straw for biolipid production using oleaginous microorganisms

Chemosphere ◽

10.1016/j.chemosphere.2020.129390 ◽

2021 ◽

Vol 269 ◽

pp. 129390

Author(s):

Hoa Thai Doan ◽

Phuong Thi Minh Nguyen ◽

Thuy Thanh Tran ◽

Thoa Kim Nguyen ◽

Man Dinh Tran ◽

...

Keyword(s):

Rice Straw ◽

Oleaginous Microorganisms ◽

Lime Pretreatment

Download Full-text

Dependence of Intergranular Fracture on Boundary Topography and Cohesion

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100071193 ◽

1973 ◽

Vol 31 ◽

pp. 150-151

Author(s):

J. E. Doherty ◽

A. F. Giamei ◽

B. H. Kear ◽

C. W. Steinke

Keyword(s):

Grain Boundary ◽

Room Temperature ◽

Nickel Base Superalloy ◽

Boundary Shear ◽

Room Temperature Ductility ◽

Solid Solution Matrix ◽

Nickel Base ◽

Solution Matrix ◽

Different Levels ◽

Base Superalloy

Recently we have been investigating a class of nickel-base superalloys which possess substantial room temperature ductility. This improvement in ductility is directly related to improvements in grain boundary strength due to increased boundary cohesion through control of detrimental impurities and improved boundary shear strength by controlled grain boundary micros true tures.For these investigations an experimental nickel-base superalloy was doped with different levels of sulphur impurity. The micros tructure after a heat treatment of 1360°C for 2 hr, 1200°C for 16 hr consists of coherent precipitates of γ’ Ni3(Al,X) in a nickel solid solution matrix.

Download Full-text

Hepatocyte Alterations in Guinea Pigs FED Polychlorinated Biphenyls (PCBs), Dibenzodioxins (PCDD), AND DIBENZOFURANS (PCDF)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053085 ◽

1982 ◽

Vol 40 ◽

pp. 56-57

Author(s):

J. N. Turner ◽

D. N. Collins

Keyword(s):

Guinea Pigs ◽

Room Temperature ◽

Dose Group ◽

Methyl Cellulose ◽

Uranyl Acetate ◽

Target Organ ◽

Office Building ◽

Lead Citrate ◽

Control Groups ◽

Cooling Fluid

A fire involving an electric service transformer and its cooling fluid, a mixture of PCBs and chlorinated benzenes, contaminated an office building with a fine soot. Chemical analysis showed PCDDs and PCDFs including the highly toxic tetra isomers. Guinea pigs were chosen as an experimental animal to test the soot's toxicity because of their sensitivity to these compounds, and the liver was examined because it is a target organ. The soot was suspended in 0.75% methyl cellulose and administered in a single dose by gavage at levels of 1,10,100, and 500mgm soot/kgm body weight. Each dose group was composed of 6 males and 6 females. Control groups included 12 (6 male, 6 female) animals fed activated carbon in methyl cellulose, 6 males fed methyl cellulose, and 16 males and 10 females untreated. The guinea pigs were sacrificed at 42 days by suffocation in CO2. Liver samples were immediately immersed and minced in 2% gluteraldehyde in cacadylate buffer at pH 7.4 and 4°C. After overnight fixation, samples were postfixed in 1% OsO4 in cacodylate for 1 hr at room temperature, embedded in epon, sectioned and stained with uranyl acetate and lead citrate.

Download Full-text