scholarly journals Effects of circadian clock and light on melatonin concentration in Hypericum perforatum L. (St. John’s Wort)

2020 ◽  
Vol 61 (1) ◽  
Author(s):  
Ming-Hsiu Chung ◽  
Tzu-Shing Deng
Keyword(s):  
High Performance ◽  
Hypericum Perforatum ◽  
Fresh Weight ◽  
Melatonin Concentration ◽  
Long Day ◽  
Spatiotemporal Regulation ◽  
Circadian Time ◽  
Hypericum Perforatum L ◽  
Pharmaceutical Industries ◽  
Oriented Research

Abstract Background Melatonin acts as a signaling hormone and entraining agent in many organisms. We studied the spatiotemporal regulation and influence of light (photoperiods, intensities, and spectral qualities) on melatonin concentration in the medicinal herb Hypericum perforatum L. Furthermore, melatonin concentrations in the leaves of eight species of the Hypericum genus were compared and analyzed using high-performance liquid chromatography. Results Melatonin concentration was found to be the highest in its flowers and leaves. The leaves exhibited a rhythmic variation in melatonin concentration of approximately 24 h under both light–dark entrained (Zeitgeber time) and constant light [circadian time (CT)] conditions, with melatonin concentration peaking at approximately CT6 in the middle of the subjective day. Melatonin concentration was influenced significantly by not only photoperiods but also applied light’s wavelength and intensity. It was approximately six times higher under long-day conditions (18-h light:6-h dark) than under short-day photoperiods (10-h light:14-h dark) and was the highest (131 μg/g fresh weight [FW]) under treatment with blue light at an intensity of 45 µmol·m2/s of photons. The melatonin concentration of the two examined Hypericum spp., namely H. kouytchense Lev. and H. coris L., were approximately twice that of H. perforatum L. Conclusion Our findings provide first insights on melatonin-related functions and mechanisms in the circadian system of H. perforatum and useful resources for further melatonin-oriented research and possible applications in agriculture and pharmaceutical industries.

scholarly journals Effects of circadian clock and light on melatonin concentration in Hypericum perforatum L. (St. John’s Wort)

2020 ◽  
Author(s):  
Chung Ming-Hsiu ◽  
Tzu-Shing Deng
Keyword(s):  
High Performance ◽  
Hypericum Perforatum ◽  
Fresh Weight ◽  
Melatonin Concentration ◽  
Long Day ◽  
Spatiotemporal Regulation ◽  
Circadian Time ◽  
Hypericum Perforatum L ◽  
Pharmaceutical Industries ◽  
Oriented Research

Abstract Background Melatonin acts as a signaling hormone and entraining agent in many organisms. We studied the spatiotemporal regulation and influence of light (photoperiods, intensities, and spectral qualities) on melatonin concentration in the medicinal herb Hypericum perforatum L. Furthermore, melatonin concentrations in the leaves of eight species of the Hypericum genus were compared and analyzed using high-performance liquid chromatography. Results Melatonin concentration was found to be the highest in its flowers and leaves. The leaves exhibited a rhythmic variation in melatonin concentration of approximately 24 h under both light–dark entrained (Zeitgeber time) and constant light [circadian time (CT)] conditions, with melatonin concentration peaking at approximately CT6 in the middle of the subjective day. Melatonin concentration was influenced significantly by not only photoperiods but also applied light’s wavelength and intensity. It was approximately six times higher under long-day conditions (18-h light:6-h dark) than under short-day photoperiods (10-h light:14-h dark) and was the highest (131 μg/g fresh weight [FW]) under treatment with blue light at an intensity of 45 µmol·m 2 /s of photons. The melatonin concentration of the two examined Hypericum spp. , namely H. kouytchense Lev. and H. coris L., were approximately twice that of H. perforatum L. Conclusion Our findings provide first insights on melatonin-related functions and mechanisms in the circadian system of H. perforatum and useful resources for further melatonin-oriented research and possible applications in agriculture and pharmaceutical industries.

scholarly journals Effects of circadian clock and light on melatonin concentration in Hypericum perforatum L. (St. John’s Wort)

2020 ◽  
Author(s):  
Chung Ming-Hsiu ◽  
Tzu-Shing Deng
Keyword(s):  
Hypericum Perforatum ◽  
Circadian System ◽  
Fresh Weight ◽  
Melatonin Concentration ◽  
Long Day ◽  
Spatiotemporal Regulation ◽  
Circadian Time ◽  
Hypericum Perforatum L ◽  
Pharmaceutical Industries ◽  
Oriented Research

Abstract Background Melatonin acts as a signaling hormone and entraining agent in many organisms. Here, we studied the spatiotemporal regulation and influence of light (photoperiods, intensities and spectral qualities) on melatonin concentration in the medicinal herb Hypericum perforatum L. Furthermore, melatonin concentration in leaves of 8 species of Hypericum genus collected has been compared and analyzed by HPLC. Results Melatonin concentration was found to be the highest in its flowers and leaves. The leaves exhibited a rhythmic variation in melatonin concentration of approximately 24 h under both light–dark entrained (Zeitgeber time) and constant light [circadian time (CT)] conditions, with melatonin concentration peaking at approximately CT6 in the middle of the subjective day. Melatonin concentration was influenced significantly by not only photoperiods but also applied light’s wavelength and intensity. It was approximately six times higher under long-day conditions (18-h light:6-h dark) than under short-day photoperiods (10-h light:14-h dark) and was the highest (131 µg/g fresh weight [FW]) under treatment with blue light at an intensity of 45 µmol·m2/s of photons. The melatonin concentration of the two examined Hypericum spp., namely H. kouytchense Lev. and H. coris L., were approximately twice that of H. perforatum L. Conclusion Our findings provide first insights on melatonin-related functions and mechanisms in the circadian system of H. perforatum and useful resources for further melatonin-oriented research and possible applications in agriculture and pharmaceutical industries.

scholarly journals Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection

2010 ◽  
Vol 8 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Abdulkadir Levent ◽  
Suat Ekin ◽  
Gökhan Oto
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hypericum Perforatum ◽  
Treated Group ◽  
Retinyl Palmitate ◽  
Rat Serum ◽  
Array Detection ◽  
Hypericum Perforatum L ◽  
Β Carotene

AbstractA new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with Hypericum Perforatum L. and 7,12-dimethylbenz[a]anthracene. Furthermore, vitamin C was determined spectrophotometrically. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-acetonitrile-tetrahydrofuran (65:30:5, v/v/v) as mobile phase, at a flow rate of 1.5 mL min−1 and 40°C. Diode-array detection was conducted at 325 and 450 nm for retinol and retinyl palmitate, and β-carotene, respectively with a running time of 26 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. This method was then applied to determine the amounts of retinol, retinyl palmitate and β-carotene in rat serum. Results of this study demonstrated that at 60th day in the 7,12-dimethylbenz[a]anthracene-treated group there was a significant decrease (pa] anthracene + Hypericum Perforatum L. treated group compared to the control group..

scholarly journals Evaluation of the influence of different doses of pre-sowing irradiation of seeds on the content of flavonoids in Hypericum perforatum L. medicinal raw material

2019 ◽  
Vol 25 ◽  
pp. 310-315 ◽  
Author(s):  
A. G. Salivon ◽  
K. V. Lystvan ◽  
S. V. Litvinov ◽  
S. A. Pchelovska ◽  
Yu. V. Shylina ◽  
...  
Keyword(s):  
High Performance ◽  
Hypericum Perforatum ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Raw Material ◽  
Flavonoid Content ◽  
X Ray ◽  
Hypericum Perforatum L ◽  
Ethanol Extracts ◽  
Different Doses

Aim. The purpose of this work was to determine the dose of g- and X-ray pre-sowing irradiation of seeds of Hypericum perforatum L., causing an increase in biomass and the accumulation of flavonoids in medicinal raw materials. Methods. X-ray and g-irradiation of seeds, biometric methods, flavonoids extraction, quantification of flavonoid content in extracts using spectrophotometry, qualitative and semi-quantitative analysis of extracts using high-performance liquid chromatography (HPLC), common statistical methods for processing and analysis of data. Results. It was shown that acute X-ray pre-sowing irradiation of H. perforatum seeds at a dose of 20 Gy leads to an increase in the crop of medicinal raw material without losing its pharmaceutical value. Irradiation also increases concentration of quercetin, routine, hyperoside in herbal medicines, grown from seeds, that was chronically irradiated with g-radiation in a total dose of 1 Gy. The HPLC analysis of extracts confirmed that the qualitative composition of ethanol extracts of H. perforatum did not change due to the effect of irradiation on the seeds. Conclusions. The obtained results confirm the possibility of application of pre-sowing irradiation of seeds of H. perforatum in the range of 1–35 Gy in order to increase its productivity and increase the pharmaceutical value of the medicinal raw material. Keywords: Hypericum perforatum L., ionizing irradiation, productivity, flavonoids.

scholarly journals The Effect of Monomers on the Recognition Properties of Molecularly Imprinted Beads for Proto-hypericin and Proto-pseudohypericin

2020 ◽  
Vol 57 (1) ◽  
pp. 100-111
Author(s):  
Ana-Mihaela (Florea) Gavrila ◽  
Tanta-Verona Iordache ◽  
Catherine Branger ◽  
Hugues Brisset ◽  
Katri Laatikainen ◽  
...  
Keyword(s):  
High Performance ◽  
Hypericum Perforatum ◽  
Suspension Polymerization ◽  
Imprinted Polymer ◽  
Visible Spectroscopy ◽  
Spectroscopy Analysis ◽  
Molecularly Imprinted ◽  
Hypericum Perforatum L ◽  
Uv Visible ◽  
The Impact

Molecularly imprinted polymer (MIP) beads for proto-hypericin recognition were prepared by suspension polymerization. In order to study the impact of monomers on the MIPs properties, various monomers such as acrylic acid (AA), hydroxyethyl methacrylate (HEMA), methacrylic acid (MAA) and itaconic acid (IA) in their combinations were crosslinked with ethylene glycol dimethacrylate (EDMA) in the presence of a complex phyto-extract template derived from Hypericum perforatum L. The synthesized MIPs and corresponding non-imprinted polymers NIPs were characterized by infrared spectroscopy analysis, morphology and thermogravimetric analysis. High-performance liquid chromatography combined with UV-Visible spectroscopy, used to investigate the recognition properties of the MIPs for various naphthodianthrones, pointed out that the MIP IA-AA system seemed to be the most adequate for favoring quantitative rebinding of proto-hypericin and proto-pseudohypericin against competitors with similar structures, like hypericin and pseudo-hypericin, which are usually present in high quantities in the primary Hypericum perforatum L. phyto-extracts.

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