Online extraction of antihypertensive drugs and their metabolites from untreated human serum samples using restricted access carbon nanotubes in a column switching liquid chromatography system

2017 ◽  
Vol 1528 ◽  
pp. 41-52 ◽  
Author(s):  
Henrique Dipe de Faria ◽  
Carolina Tosin Bueno ◽  
Jose Eduardo Krieger ◽  
Eduardo Moacyr Krieger ◽  
Alexandre Costa Pereira ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Liquid Chromatography ◽  
Human Serum ◽  
Antihypertensive Drugs ◽  
Column Switching ◽  
Serum Samples ◽  
Restricted Access ◽  
Human Serum Samples ◽  
Online Extraction ◽  
Liquid Chromatography System

scholarly journals A Novel, Rapid, and Low-Volume Assay for Therapeutic Drug Monitoring of Posaconazole and Other Long-Chain Azole-Class Antifungal Drugs

mSphere ◽  
2018 ◽  
Vol 3 (6) ◽  
Author(s):  
Gregory R. Wiedman ◽  
Yanan Zhao ◽  
David S. Perlin
Keyword(s):  
Liquid Chromatography ◽  
Therapeutic Drug Monitoring ◽  
Human Serum ◽  
Drug Monitoring ◽  
Fungal Infections ◽  
Antifungal Drugs ◽  
Therapeutic Drug ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Long Chain

ABSTRACT Clinicians need a better way to accurately monitor the concentration of antimicrobials in patient samples. In this report, we describe a novel, low-sample-volume method to monitor the azole-class antifungal drug posaconazole, as well as certain other long-chain azole-class antifungal drugs in human serum samples. Posaconazole represents an important target for therapeutic drug monitoring (TDM) due to its widespread use in treating invasive fungal infections and well-recognized variability of pharmacokinetics. The current “gold standard” requires trough and peak monitoring through high-pressure liquid chromatography (HPLC) or liquid chromatography-tandem mass spectroscopy (LC-MS/MS). Other methods include bioassays that use highly susceptible strains of fungi in culture plates or 96-well formats to monitor concentrations. Currently, no method exists that is both highly accurate in detecting free drug concentrations and is also rapid. Herein, we describe a new method using reduced graphene oxide (rGO) and a fluorescently labeled aptamer, which can accurately assess clinically relevant concentrations of posaconazole and other long-chain azole-class drugs in little more than 1 h in a total volume of 100 µl. IMPORTANCE This work describes an effective assay for TDM of long-chain azole-class antifungal drugs that can be used in diluted human serum samples. This assay will provide a quick, cost-effective method for monitoring concentrations of drugs such as posaconazole that exhibit well-documented pharmacokinetic variability. Our rGO-aptamer assay has the potential to improve health care for those struggling to treat fungal infections in rural or resource-limited setting.

Determination of ciprofloxacin and enoxacin in human serum samples by micellar liquid chromatography

2004 ◽  
Vol 516 (1-2) ◽  
pp. 135-140 ◽  
Author(s):  
José Luis Vı́lchez ◽  
Lilia Araujo ◽  
Avismelsi Prieto ◽  
Alberto Navalón
Keyword(s):  
Liquid Chromatography ◽  
Human Serum ◽  
Micellar Liquid Chromatography ◽  
Serum Samples ◽  
Human Serum Samples

Characterization and application of restricted access carbon nanotubes in online extraction of anticonvulsant drugs from plasma samples followed by liquid chromatography analysis

2017 ◽  
Vol 1054 ◽  
pp. 50-56 ◽  
Author(s):  
Rodrigo Campos dos Santos ◽  
Adriana Kaori Kakazu ◽  
Mariane Gonçalves Santos ◽  
Fábio Antônio Belinelli Silva ◽  
Eduardo Costa Figueiredo
Keyword(s):  
Carbon Nanotubes ◽  
Liquid Chromatography ◽  
Anticonvulsant Drugs ◽  
Plasma Samples ◽  
Restricted Access ◽  
Chromatography Analysis ◽  
Online Extraction

Simultaneous measurement of 13 circulating vitamin D3 and D2 mono and dihydroxy metabolites using liquid chromatography mass spectrometry

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Carl Jenkinson ◽  
Reena Desai ◽  
Andrzej T. Slominski ◽  
Robert C. Tuckey ◽  
Martin Hewison ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Vitamin D ◽  
Liquid Chromatography ◽  
Human Serum ◽  
High Sensitivity ◽  
Reference Level ◽  
Vitamin D Metabolites ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Limit Of Quantitation

Abstract Objectives Clinical evaluation of vitamin D status is conventionally performed by measuring serum levels of a single vitamin D metabolite, 25-hydroxyvitamin D predominantly by immunoassay methodology. However, this neglects the complex metabolic pathways involved in vitamin D bioactivity, including two canonical forms D3 and D2, bioactive 1,25-dihydroxy metabolites and inactive 24-hydroxy and other metabolites. Methods Liquid chromatography-tandem mass spectrometry (LC-MS/MS) can measure multiple analytes in a sample during a single run with high sensitivity and reference level specificity. We therefore aimed to develop and validate a LC-MS/MS method to measure simultaneously 13 circulating vitamin D metabolites and apply it to 103 human serum samples. Results The LC-MS/MS method using a Cookson-type derivatization reagent phenyl-1,2,4-triazoline-3,5-dione (PTAD) quantifies 13 vitamin D metabolites, including mono and dihydroxy-metabolites, as well as CYP11A1-derived D3 and D2 metabolites in a single run. The lower limit of quantitation was 12.5 pg/mL for 1,25(OH)2D3 with accuracy verified by analysis of National Institute of Standards and Technology (NIST) 972a standards. Quantification of seven metabolites (25(OH)D3, 25(OH)D2, 3-epi-25(OH)D3, 20(OH)D3, 24,25(OH)2D3, 1,25(OH)2D3 and 1,20S(OH)2D3) was consistently achieved in human serum samples. Conclusions This profiling method can provide new insight into circulating vitamin D metabolite pathways forming the basis for improved understanding of the role of vitamin D in health and disease.

Sign in / Sign up

Export Citation Format

Share Document