Size-exclusion chromatography using reverse-phase columns for protein separation

2018 ◽  
Vol 1571 ◽  
pp. 201-212 ◽  
Author(s):  
Tung-Yung Huang ◽  
Lang-Ming Chi ◽  
Kun-Yi Chien
Keyword(s):  
Size Exclusion Chromatography ◽  
Protein Separation ◽  
Size Exclusion ◽  
Reverse Phase ◽  
Exclusion Chromatography

Microfluidic Size Exclusion Chromatography (μSEC) for Extracellular Vesicles and Plasma Protein Separation

Small ◽  
2022 ◽  
pp. 2104470
Author(s):  
Sheng Yuan Leong ◽  
Hong Boon Ong ◽  
Hui Min Tay ◽  
Fang Kong ◽  
Megha Upadya ◽  
...  
Keyword(s):  
Size Exclusion Chromatography ◽  
Extracellular Vesicles ◽  
Plasma Protein ◽  
Protein Separation ◽  
Size Exclusion ◽  
Exclusion Chromatography

scholarly journals Multidimensional profiling of plasma lipoproteins by size exclusion chromatography followed by reverse-phase protein arrays

2011 ◽  
Vol 52 (12) ◽  
pp. 2323-2331 ◽  
Author(s):  
Gregor Dernick ◽  
Stefan Obermüller ◽  
Cyrill Mangold ◽  
Christine Magg ◽  
Hugues Matile ◽  
...  
Keyword(s):  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Plasma Lipoproteins ◽  
Reverse Phase ◽  
Protein Arrays ◽  
Phase Protein ◽  
Exclusion Chromatography

scholarly journals Purification of bacteriocins using size-exclusion chromatography

2016 ◽  
Vol 11 (2) ◽  
pp. 281 ◽  
Author(s):  
Vivek K. Bajpai ◽  
Irfan A. Rather ◽  
Alshammari Fanar Hamad
Keyword(s):  
Mass Spectrometry ◽  
Ammonium Sulfate ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Ammonium Sulfate Precipitation ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Cell Free Supernatant ◽  
Video Clips ◽  
Exclusion Chromatography

<p>The bacteriocin purification involves following main steps. a). Extraction of cell-free-supernatant of bacteria. b). Ammonium sulfate precipitation. c). Dialysis. d). Diafiltration using PVP and e). Size-exclusion chromatography. However, depending on the nature of work, the compound could be further analyzed by reverse-phase HPLC, NMR, mass spectrometry and sequencing.</p><p><strong>Video Clips</strong></p><p><a href="https://youtube.com/v/u1BmWfOTS9w">Part 1</a>: 4 min 52 sec</p><p><a href="https://youtube.com/v/aF45JFnwErc">Part 2</a>: 1 min 47 sec</p>

Size exclusion chromatography of polysaccharides with reverse phase liquid chromatography

2014 ◽  
Vol 1323 ◽  
pp. 97-103 ◽  
Author(s):  
Yan He ◽  
Weiying Hou ◽  
Melissa Thompson ◽  
Heidi Holovics ◽  
Tracy Hobson ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Reverse Phase ◽  
Reverse Phase Liquid Chromatography ◽  
Exclusion Chromatography ◽  
Phase Liquid

Contribution à l'étude du polymorphisme de quelques métallothionéines par chromatographie liquide de haute performance

1994 ◽  
Vol 72 (5) ◽  
pp. 1238-1245 ◽  
Author(s):  
Guy Bordin ◽  
Fernando Cordeiro Raposo ◽  
Adela-Rosa Rodriguez
Keyword(s):  
Size Exclusion Chromatography ◽  
Rabbit Liver ◽  
Size Exclusion ◽  
Reverse Phase ◽  
Reverse Phase Chromatography ◽  
Exclusion Chromatography

Four commercially available metallothioneins from rabbit liver (RL, RL I, RL II) and from horse kidney (HK) have been studied by size exclusion chromatography and by reverse-phase chromatography, to characterize their polymorphism. The isoforms are detected by UV at 250 nm. Separations by size exclusion show that the metallothioneins are partially dimerized, with molar rates of dimer ranging from 5% for HK to 15% for RL II. The four chromatograms obtained by reverse phase exhibit a different morphology: RL separates in four main isoforms, RL I into two, RL II into three, and HK into two. An attribution of the various peaks for the RL, RL I, and RL II series is proposed. The comparable results obtained by the two methods allow us to estimate that RL is composed of 20–30% RL I and 70–80% of RL II.

Columns switch recycling size exclusion chromatography for high resolution protein separation

2009 ◽  
Vol 1216 (42) ◽  
pp. 7024-7032 ◽  
Author(s):  
Huiming Yuan ◽  
Lihua Zhang ◽  
Weibing Zhang ◽  
Zhen Liang ◽  
Yukui Zhang
Keyword(s):  
High Resolution ◽  
Size Exclusion Chromatography ◽  
Protein Separation ◽  
Size Exclusion ◽  
Exclusion Chromatography
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