Antibiotic resistance and phylogenetic comparison of human, pet animals and raw milk Staphylococcus aureus isolates

2021 ◽  
pp. 101717
Author(s):  
Sara Pahlavanzadeh ◽  
Rahem Khoshbakht ◽  
Hami Kaboosi ◽  
Elham Moazamian
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Raw Milk ◽  
Phylogenetic Comparison

Assessing and analysing contamination of a dairy products processing plant byStaphylococcus aureususing antibiotic resistance and PFGE

2000 ◽  
Vol 46 (12) ◽  
pp. 1108-1114 ◽  
Author(s):  
E C Tondo ◽  
MC M Guimarães ◽  
J AP Henriques ◽  
M AZ Ayub
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antibiotic Susceptibility ◽  
Dairy Products ◽  
Dairy Product ◽  
Raw Milk ◽  
Food Handler ◽  
Processing Plant ◽  
Pfge Analysis ◽  
Antibiotic Susceptibility Test

A dairy product processing plant was studied for 2.5 years to examine contamination with Staphylococcus aureus and try to correlate the source of contamination. Cultures were submitted to an antibiotic susceptibility test (AST) and characterised by Pulsed-field Gel Electrophoresis (PFGE) analysis. Results showed that 35.2% (19/51) of food handlers were asymptomatic carriers of S. aureus, and that 90.4% (19/21) of raw milk sampled was contaminated. Staphylococcus aureus was isolated from only 10 samples among more than 3200 investigated dairy products. No S. aureus contamination was found on machinery. The AST analysis demonstrated sensitivity of tested S. aureus to oxacillin, cephalothin, vancomycin, gentamicin, and sulfamethoxazole/trimethoprim. AST analysis generated eight different phenotypic profiles, but did not allow us to identify the source of contamination in seven of ten final products. PFGE analysis proved to be a sensitive method as it generated 42 different DNA banding profiles among the 48 S. aureus investigated, demonstrating a lack of predominance of endemic strains in the plant, contrary to suggestions raised by antibiotic resistance typing. Based on PFGE genotyping, S. aureus strains isolated from four contaminated final products were similar to four S. aureus isolated from raw milk. Five final products contained S. aureus different from all other strains collected, and one showed similarity to a strain isolated from a food handler. These results suggest contamination by raw milk as the main source of contamination of the final dairy products.Key words: Staphylococcus aureus, dairy products, antibiotic susceptibility, PFGE.

scholarly journals Antimicrobial Drug Resistance of Escherichia coli and Staphylococcus aureus Isolated from Milk and Milk Based Beverages of Dhaka City, Bangladesh

2021 ◽  
Author(s):  
Khondaker Wahid Hasan ◽  
Nourin Tarannum ◽  
Sahana Parveen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Raw Milk ◽  
Penicillin G ◽  
Resistance Pattern ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Antibiotic Resistance Pattern ◽  
And Staphylococcus Aureus

The increase of pathogens in milk is threatening for the human beings. This is an investigation on overall microbiological analysis of raw and pasteurized milk as well as the milk-based beverages and also determining the antibiotic resistance pattern of isolated Escherichia coli and Staphylococcus aureus. A total of 100 samples (raw milk, pasteurized milk, mattha, lassi and laban) were taken from various locations of the capital city of Bangladesh, Dhaka. Total Viable Count, Total Coliform Count and Yeast and Moulds Count were performed as the microbiological inspection of selected samples. E.coli and Staphylococcus aureus were identified by conducting morphological analysis, gram-staining and biochemical tests. Antibiotic resistance pattern of isolated Escherichia Coli and Staphylococcus aureus were also detected with 11 commonly used antibiotics by conducting disc-diffusion method, following the CLSI guideline. The TVC range was the highest in raw milk samples (3.8×104 – 4.1×108 cfu/ml), and the lowest in pasteurized milk samples (1.2×102 – 5.4×103 cfu/ml), while 70% raw milk and 10% pasteurized milk samples strains were above the acceptable limit of Food and Drug Administration (FDA). Thirty-six Escherichia coli and thirty-two Staphylococcus aureus were isolated from all the 100 milk and milk-based beverage samples. The isolated Escherichia coli strains were most resistant to Penicillin G (81.58%), Erythromycin (78.94%) and Ampicillin (73.68%), and isolated Staphylococcus aureus strains were most resistant to Penicillin G (90.62%), Ampicillin (81.25%) and Methicillin (71.87%). respectively. Public awareness is needed to reduce the redundant use of antibiotics.

scholarly journals Antibiotic resistance of Staphylococcus aureus isolated from raw cow and goat milk produced in the Tiaret and Souk Ahras areas of Algeria

2021 ◽  
pp. 1929-1934
Author(s):  
Sofiane Tamendjari ◽  
Farida Afri Bouzebda ◽  
Lina Chaib ◽  
Hebib Aggad ◽  
Mohammed Ramdani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Health Hazard ◽  
Food Poisoning ◽  
Goat Milk ◽  
Raw Milk ◽  
Penicillin G ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Adequate Treatment

Background and Aim: Staphylococcus aureus is a leading cause of infection in both humans and animals. Most livestock strains have shown antibiotic resistance to the many molecules used in veterinary therapeutics. This study aimed to assess the resistance patterns of these bacteria, we carried out our study in the Tiaret and Souk Ahras areas of Algeria. Materials and Methods: We collected 116 samples of bovine and goat milk to detect S. aureus. We used a selective media to isolate the strains, followed by biochemically identifying the isolates. We determined the susceptibility of the strains to antibiotic molecules using the disk diffusion method and confirmed the methicillin-resistant S. aureus (MRSA) with oxacillin minimum inhibitory concentration (MIC). Results: Our results showed that 26.72% of the samples were contaminated with S. aureus, and we recovered 31 isolates from the positive samples. We ascribed a high resistance profile to penicillin G (96.77%), fusidic acid (67.74%), and tobramycin (45.16%) and isolated 4MRSA strains. Conclusion: The presence of S. aureus, including MRSA strains in raw milk, can present a public health hazard, because these strains can cause widespread food poisoning . This finding will be useful to the veterinarians to choose an adequate treatment and to sensitize livestock breeders and milk producers to ensure the health of consumers.

scholarly journals Antibiotic Resistance and Detection of femA Gene in Staphylococcus Aureus Isolates from Raw Milk

2016 ◽  
Vol 10 (4) ◽  
pp. 40-45
Author(s):  
Pouya Khodadadi ◽  
Mehdi Bizhanzadeh ◽  
Akram Najafi ◽  
Vajiheh Zarinpour ◽  
Abdolali Moshfe ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Raw Milk

scholarly journals Prevalence of the Enterotoxigenic Staphylococcus Aureus Strains Isolated from Raw Milk and Cheese Produced in North Macedonia

2021 ◽  
Vol 44 (1) ◽  
pp. 71-77
Author(s):  
Marija Ratkova Manovska ◽  
Mirko Prodanov ◽  
Ljupcho Angelovski ◽  
Dean Jankuloski ◽  
Pavle Sekulovski
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Detection Limit ◽  
Scientific Literature ◽  
Foodborne Pathogen ◽  
Food Poisoning ◽  
Raw Milk ◽  
Microbiological Criteria ◽  
Possible Cause ◽  
Raw Cow’S Milk

Abstract Staphylococcus aureus is an important foodborne pathogen due to toxin-related virulence, invasiveness and antibiotic resistance. The ability of S. aureus strains to produce one or more staphylococcal enterotoxins (SEs) in food has been associated with the occurrence of staphylococcal food poisoning (SFP), which is the most common foodborne intoxication worldwide. The study aimed to determine the count of S. aureus strains in samples of raw cow’s milk and various cheeses produced in R. North Macedonia and to detect their ability to produce enterotoxins by passive agglutination SET RPLA (OXOID, UK) and by enzyme-linked fluorescence assay (ELFA) VIDAS SET 2 (Biomerieux, France). A total of 130 S. aureus strains were analyzed. The ability to produce SEs was determined in 17 (13.1%) strains using the SET RPLA detection kit and in 20 (15.4%) strains using the VIDAS SET 2. The study detected enterotoxigenic strains in cheese samples, despite the low count of S. aureus which was below the detection limit according to the Book of rules for microbiological criteria (Off. G. of R.M no 100/2013). Based on these and similar findings, S. aureus must be considered as a possible cause of intoxication, despite the undetected and underreported cases of SFP in the scientific literature.

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