scholarly journals Continuous purification and culture of rat type 1 and type 2 alveolar epithelial cells by magnetic cell sorting

2021 ◽  
Author(s):  
Di Liu ◽  
Jian-Hui Sun ◽  
Hua-Cai Zhang ◽  
Jian-Xin Jiang ◽  
Ling Zeng
Keyword(s):  
Epithelial Cells ◽  
Cell Sorting ◽  
Alveolar Epithelial Cells ◽  
Magnetic Cell Sorting ◽  
Alveolar Epithelial

scholarly journals Wnt signaling regulates trans-differentiation of stem cell like type 2 alveolar epithelial cells to type 1 epithelial cells

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Elhusseiny Mohamed Mahmud Abdelwahab ◽  
Judit Rapp ◽  
Diana Feller ◽  
Veronika Csongei ◽  
Szilard Pal ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Wnt Signaling ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial

scholarly journals Adipose Tissue-Derived Stem Cells Have the Ability to Differentiate into Alveolar Epithelial Cells and Ameliorate Lung Injury Caused by Elastase-Induced Emphysema in Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Eriko Fukui ◽  
Soichiro Funaki ◽  
Kenji Kimura ◽  
Toru Momozane ◽  
Atsuomi Kimura ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Epithelial Cells ◽  
Lung Injury ◽  
Alveolar Epithelial Cells ◽  
Chronic Obstructive ◽  
Alveolar Cells ◽  
Alveolar Epithelial

Chronic obstructive pulmonary disease is a leading cause of mortality globally, with no effective therapy yet established. Adipose tissue-derived stem cells (ADSCs) are useful for ameliorating lung injury in animal models. However, whether ADSCs differentiate into functional cells remains uncertain, and no study has reported on the mechanism by which ADSCs improve lung functionality. Thus, in this study, we examined whether ADSCs differentiate into lung alveolar cells and are able to ameliorate lung injury caused by elastase-induced emphysema in model mice. Here, we induced ADSCs to differentiate into type 2 alveolar epithelial cells in vitro. We demonstrated that ADSCs can differentiate into type 2 alveolar epithelial cells in an elastase-induced emphysematous lung and that ADSCs improve pulmonary function of emphysema model mice, as determined with spirometry and 129Xe MRI. These data revealed a novel function for ADSCs in promoting repair of the damaged lung by direct differentiation into alveolar epithelial cells.

Apoptosis and DNA damage in type 2 alveolar epithelial cells cultured from hyperoxic rats

1998 ◽  
Vol 274 (5) ◽  
pp. L714-L720 ◽  
Author(s):  
Sue Buckley ◽  
Lora Barsky ◽  
Barbara Driscoll ◽  
Kenneth Weinberg ◽  
Kathryn D. Anderson ◽  
...  
Keyword(s):  
Dna Damage ◽  
Epithelial Cells ◽  
Cellular Response ◽  
Ex Vivo ◽  
Keratinocyte Growth Factor ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Dna Laddering

Apoptosis is a genetically controlled cellular response to developmental stimuli and environmental insult that culminates in cell death. Sublethal hyperoxic injury in rodents is characterized by a complex but reproducible pattern of lung injury and repair during which the alveolar surface is damaged, denuded, and finally repopulated by type 2 alveolar epithelial cells (AEC2). Postulating that apoptosis might occur in AEC2 after hyperoxic injury, we looked for the hallmarks of apoptosis in AEC2 from hyperoxic rats. A pattern of increased DNA end labeling, DNA laddering, and induction of p53, p21, and Bax proteins, strongly suggestive of apoptosis, was seen in AEC2 cultured from hyperoxic rats when compared with control AEC2. In contrast, significant apoptosis was not detected in freshly isolated AEC2 from oxygen-treated rats. Thus the basal culture conditions appeared to be insufficient to ensure the ex vivo survival of AEC2 damaged in vivo. The oxygen-induced DNA strand breaks were blocked by the addition of 20 ng/ml of keratinocyte growth factor (KGF) to the culture medium from the time of plating and were partly inhibited by Matrigel or a soluble extract of Matrigel. KGF treatment resulted in a partial reduction in the expression of the p21, p53, and Bax proteins but had no effect on DNA laddering. We conclude that sublethal doses of oxygen in vivo cause damage to AEC2, resulting in apoptosis in ex vivo culture, and that KGF can reduce the oxygen-induced DNA damage. We speculate that KGF plays a role as a survival factor in AEC2 by limiting apoptosis in the lung after acute hyperoxic injury.

Isolation and Identification of Type 2 Alveolar Epithelial Cells

CHEST Journal ◽  
1975 ◽  
Vol 67 (2) ◽  
pp. 36S-37S ◽  
Author(s):  
Robert Mason ◽  
Mary C. Williams ◽  
John A. Clements
Keyword(s):  
Epithelial Cells ◽  
Alveolar Epithelial Cells ◽  
Isolation And Identification ◽  
Alveolar Epithelial

Role Of The Type 2 Alveolar Epithelial Cells In An Experimental Model Of Acute Lung Injury

2012 ◽  
Author(s):  
Sonia Garcia-Hernandez ◽  
Ricardo Gutierrez ◽  
Lucio Diaz-Flores ◽  
Jesus Villar ◽  
Francisco Valladares
Keyword(s):  
Acute Lung Injury ◽  
Epithelial Cells ◽  
Lung Injury ◽  
Experimental Model ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial
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