Fabrication of fluorescent microparticles by doping water-soluble CdTe nanocrystals into calcium carbonate for monitoring intracellular uptake

ABSTRACT Achromatium oxaliferum is a large sulfur bacterium easily recognized by large intracellular calcium carbonate bodies. Although these bodies often fill major parts of the cells’ volume, their role and specific intracellular location are unclear. In this study, we used various microscopy and staining techniques to identify the cell compartment harboring the calcium carbonate bodies. We observed that Achromatium cells often lost their calcium carbonate bodies, either naturally or induced by treatments with diluted acids, ethanol, sodium bicarbonate and UV radiation which did not visibly affect the overall shape and motility of the cells (except for UV radiation). The water-soluble fluorescent dye fluorescein easily diffused into empty cavities remaining after calcium carbonate loss. Membranes (stained with Nile Red) formed a network stretching throughout the cell and surrounding empty or filled calcium carbonate cavities. The cytoplasm (stained with FITC and SYBR Green for nucleic acids) appeared highly condensed and showed spots of dissolved Ca2+ (stained with Fura-2). From our observations, we conclude that the calcium carbonate bodies are located in the periplasm, in extra-cytoplasmic pockets of the cytoplasmic membrane and are thus kept separate from the cell's cytoplasm. This periplasmic localization of the carbonate bodies might explain their dynamic formation and release upon environmental changes.

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Enhanced photoluminescence of water-soluble CdTe nanocrystals induced by light and pyridine

Colloids and Surfaces A Physicochemical and Engineering Aspects ◽

10.1016/j.colsurfa.2014.10.016 ◽

2015 ◽

Vol 465 ◽

pp. 54-58 ◽

Cited By ~ 5

Author(s):

Tao Duan ◽

Yu Liu ◽

Heng Zhang ◽

Jianguang Jia ◽

Xiaowen Zhou

Keyword(s):

Water Soluble ◽

Enhanced Photoluminescence ◽

Cdte Nanocrystals

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Facile One-Pot Synthesis of Luminescent, Water-Soluble, and Biocompatible Glutathione-Coated CdTe Nanocrystals

Small ◽

10.1002/smll.200500530 ◽

2006 ◽

Vol 2 (6) ◽

pp. 747-751 ◽

Cited By ~ 177

Author(s):

Huifeng Qian ◽

Chaoqing Dong ◽

Jifang Weng ◽

Jicun Ren

Keyword(s):

Water Soluble ◽

One Pot ◽

One Pot Synthesis ◽

Cdte Nanocrystals

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An effective and simple oxygen nanosensor made from MPA-capped water soluble CdTe nanocrystals

Nanotechnology ◽

10.1088/0957-4484/24/1/015501 ◽

2012 ◽

Vol 24 (1) ◽

pp. 015501 ◽

Cited By ~ 12

Author(s):

Pick Chung Lau ◽

Robert A Norwood ◽

Masud Mansuripur ◽

Nasser Peyghambarian

Keyword(s):

Water Soluble ◽

Cdte Nanocrystals

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Controlled optical properties of water-soluble CdTe nanocrystals via anion exchange

Journal of Colloid and Interface Science ◽

10.1016/j.jcis.2015.10.020 ◽

2016 ◽

Vol 463 ◽

pp. 69-74 ◽

Cited By ~ 4

Author(s):

Jing Li ◽

Jianguang Jia ◽

Yuan Lin ◽

Xiaowen Zhou

Keyword(s):

Optical Properties ◽

Anion Exchange ◽

Water Soluble ◽

Cdte Nanocrystals

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The Synthesis of Europium-Doped Calcium Carbonate by an Eco-Method as Free Radical Generator Under Low-Intensity Ultrasonic Irradiation for Body Sculpture

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.765630 ◽

2021 ◽

Vol 9 ◽

Author(s):

Che-Yung Kuan ◽

Yu-Ying Lin ◽

I-Hsuan Yang ◽

Ching-Yun Chen ◽

Chih-Ying Chi ◽

...

Keyword(s):

Crystal Structure ◽

Electron Microscope ◽

Calcium Carbonate ◽

The Body ◽

Water Soluble ◽

Tetrazolium Salt ◽

Invasive Treatment ◽

Low Intensity ◽

Low Intensity Ultrasound ◽

Body Sculpture

Body sculpture is a common method to remove excessive fat. The diet and exercise are the first suggestion to keep body shape; however, those are difficult to keep adherence. Ultrasound has been developed for fat ablation; however, it could only serve as the side treatment along with liposuction. In the study, a sonosensitizer of europium-doped calcium carbonate (CaCO3: Eu) would be synthesized by an eco-method and combined with low-intensity ultrasound for lipolysis. The crystal structure of CaCO3: Eu was identified by x-ray diffractometer (XRD). The morphology of CaCO3: Eu was analyzed by scanning electron microscope (SEM). The chemical composition of CaCO3: Eu was evaluated by energy-dispersed spectrophotometer (EDS) and inductively coupled plasma mass spectrometer (ICP-MS). The electronic diffraction pattern was to further check crystal structure of the synthesized individual grain by transmission electron microscope (TEM). The particle size was determined by Zeta-sizer. Water-soluble tetrazolium salt (WST-1) were used to evaluate the cell viability. Chloromethyl-2′,7′-dichlorofluorescein diacetate (CM-H2DCFDA) and live/dead stain were used to evaluate feasibility in vitro. SD-rat was used to evaluate the safety and efficacy in vivo. The results showed that CaCO3: Eu had good biocompatibility and could produce reactive oxygen species (ROS) after treated with low-intensity ultrasound. After 4-weeks, the CaCO3: Eu exposed to ultrasound irradiation on SD rats could significantly decrease body weight, waistline, and subcutaneous adipose tissue. We believe that ROS from sonoluminescence, CO2-bomb and locally increasing Ca2+ level would be three major mechanisms to remove away adipo-tissue and inhibit adipogenesis. We could say that the combination of the CaCO3: Eu and low-intensity ultrasound would be a non-invasive treatment for the body sculpture.

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Validation of a Fertilizer Potential Acidity Model to Predict the Effects of Water-soluble Fertilizer on Substrate pH

HortScience ◽

10.21273/hortsci.49.8.1061 ◽

2014 ◽

Vol 49 (8) ◽

pp. 1061-1066 ◽

Cited By ~ 3

Author(s):

Paul R. Fisher ◽

William R. Argo ◽

John A. Biernbaum

Keyword(s):

Calcium Carbonate ◽

Plant Species ◽

Ammonium Nitrogen ◽

Water Soluble ◽

Ph Change ◽

Bedding Plant ◽

Stable Substrate ◽

Soluble Fertilizer ◽

Substrate Ph ◽

Over Time

Two experiments were run to validate a “Nitrogen Calcium Carbonate Equivalence (CCE)” model that predicts potential fertilizer basicity or acidity based on nitrogen (N) form and concentration for floriculture crops grown with water-soluble fertilizer in containers with minimal leaching. In one experiment, nine bedding plant species were grown for 28 days in a peat-based substrate using one of three nutrient solutions (FS) composed of three commercially available water-soluble fertilizers that varied in ammonium to nitrate (NH4+:NO3–) ratio (40:60, 25:75, or 4:96) mixed with well water with 130 mg·L−1 calcium carbonate (CaCO3) alkalinity. Both the ammonium-nitrogen (NH4-N) content of the FS and plant species affected substrate pH. Predicted acidity or basicity of the FS for Impatiens walleriana Hook.f. (impatiens), Petunia ×hybrida E. Vilm. (petunia), and Pelargonium hortorum L.H. Bailey (pelargonium) from the Nitrogen CCE model was similar to observed pH change with an adjusted R2 of 0.849. In a second experiment, water alkalinity (0 or 135.5 mg·L−1 CaCO3), NH4+:NO3– ratio (75:25 or 3:97), and N concentration (50, 100, or 200 mg·L−1 N) in the FS were varied with impatiens. As predicted by the N CCE model, substrate pH decreased as NH4+ concentration increased and alkalinity decreased with an adjusted R2 of 0.763. Results provide confidence in the N CCE model as a tool for fertilizer selection to maintain stable substrate pH over time. The limited scope of these experiments emphasizes the need for more research on plant species effects on substrate pH and interactions with other factors such as residual limestone and substrate components to predict pH dynamics of containerized plants over time.

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A Study on Prescription Pattern of Multivitamins in Type-2 Diabetes Mellitus in Tertiary Care Hospital

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i3-s.4157 ◽

2020 ◽

Vol 10 (3-s) ◽

pp. 91-97

Author(s):

A B Anila ◽

P. Shaji Ancy ◽

B S SREELEKSHMI ◽

NM Mahesh ◽

B A Vishwanath

Keyword(s):

Diabetes Mellitus ◽

Calcium Carbonate ◽

Dose Range ◽

Tertiary Care ◽

Oral Route ◽

Water Soluble ◽

Diabetic Patients ◽

Care Hospital ◽

Prescribing Pattern

Aim of the study: To study the prescribing pattern of multivitamins in type-2 DM in tertiary care hospitals. Materials and methods: Human ethical approval was taken from human ethical committee, ABIPER Bangalore. The diabetic patients who satisfied the study criteria were enrolled. The patients data’s were collected in specially designed documentation form. The collected data’s were assessed using descriptive statistics. Results: The study was conducted in 158 patients in which most prescriptions [72.17%] contains water soluble vitamins+minerals. Multivitamins with or without minerals were prescribed for 90[79.76%] and 30[20.24%] days respectively. Multivitamins [100%] were given orally once a day. For indication fatigue [29.11%], water soluble vitamins+minerals were prescribed mostly. Many DM-2 patients were prescribed with water soluble vitamins+minerals which contain vitaminC and calcium carbonate as main constituents. Some of multivitamins doses were showing compliance as per FDA dose recommendations. Tablet formulation containing fat soluble vitamins+minerals[25.31%] were prescribed mostly. Conclusions: Mostly water soluble vitamins+minerals were prescribed for DM-2 patients. Multivitamins with or without minerals were prescribed for 90and30 days respectively. All multivitamins were prescribed in oral route. Mostly water soluble vitamins+minerals were prescribed for fatigue illness associated with DM-2 in which vitamin C and calcium carbonate were the main constituents. Some of the prescribed multivitamin doses were within the FDA recommended dose range. Tablet formulations were frequently prescribed with oral anti-diabetic drugs. Keywords: Multivitamins, Diabetes mellitus, Hypertension, Prescription.

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