Comparison of different commercially available cationic liposome–DNA lipoplexes: Parameters influencing toxicity and transfection efficiency

2009 ◽  
Vol 68 (2) ◽  
pp. 136-144 ◽  
Author(s):  
A. Masotti ◽  
G. Mossa ◽  
C. Cametti ◽  
G. Ortaggi ◽  
A. Bianco ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Cationic Liposome

Delivery of miRNA Using Fe2O3 Nanoparticles Capped Polyethyleneimine as a Nonviral Carrier

2012 ◽  
Vol 531-532 ◽  
pp. 543-546
Author(s):  
Gao Feng Liang ◽  
Ping Li ◽  
Wan Jun Lei
Keyword(s):  
Delivery System ◽  
Hepg2 Cells ◽  
Transfection Efficiency ◽  
Weight Ratio ◽  
Cationic Liposome ◽  
Expression Plasmid ◽  
Safe Delivery ◽  
Rna Interfering ◽  
Dna Complex ◽  
Gfp Tag

An efficient and safe delivery system of RNA interfering is required for clinical application of gene therapy. The study aimed to develop Fe2O3-based nanoparticles for gene delivery to overcome the disadvantages of polyethyleneimine (PEI) or cationic liposome as gene carrier including the cytotoxicity caused by positive charge and aggregation in the cells surface. PEI-capped Fe2O3 nanoparticles are successfully manufactured utilizing Fe2O3 as core, PEI as carapace, which bind miRNA at an appropriate weight ratio by electrostatic interaction and result in well-dispersed nanoparticles. The synthesized GFP tag with miR-26a expression plasmid was used for monitoring transfection efficiency in HepG2 cells. The nanocomplex exhibited higher transfection efficiency and lower cytotoxicity in HepG2 cells than the PEI/DNA complex and commercially available liposome. The delivery resulted in a significantly upregulation of miR-26a in HepG2 cells. Our results offer an alternate delivery system for RNA interfering that can be used on any gene of interest.

Successful Transfection of Lymphocytes by Ternary Lipoplexes

1999 ◽  
Vol 19 (6) ◽  
pp. 601-609 ◽  
Author(s):  
S. Simões ◽  
V. Slepushkin ◽  
R. Gaspar ◽  
M. C. Pedroso de Lima ◽  
N. Düzgünes
Keyword(s):  
Transgene Expression ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Cationic Liposome ◽  
Ternary Complexes ◽  
Lymphoid Cells ◽  
Charge Ratio ◽  
Cell Specificity ◽  
Lymphocytic Cell ◽  
Positively Charged

Transgene expression in lymphoid cells may be useful for modulating immune responses in, and gene therapy of, cancer and AIDS. Although cationic liposome-DNA complexes (lipoplexes) present advantages over viral vectors, they have low transfection efficiency, unfavorable features for intravenous administration, and lack of target cell specificity. The use of a targeting ligand (transferrin), or an endosome-disrupting peptide, in ternary complexes with liposomes and a luciferase plasmid, significantly promoted transgene expression in several T- and B-lymphocytic cell lines. The highest levels of luciferase activity were obtained at a lipid/DNA (±) charge ratio of 1/1, where the ternary complexes were net negatively charged. The use of such negatively charged ternary complexes may alleviate some of the drawbacks of highly positively charged plain lipoplexes for gene delivery.

scholarly journals Uptake and transfection efficiency of PEGylated cationic liposome–DNA complexes with and without RGD-tagging

Biomaterials ◽  
2014 ◽  
Vol 35 (18) ◽  
pp. 4996-5005 ◽  
Author(s):  
Ramsey N. Majzoub ◽  
Chia-Ling Chan ◽  
Kai K. Ewert ◽  
Bruno F.B. Silva ◽  
Keng S. Liang ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Cationic Liposome ◽  
Dna Complexes

Improvement of antisense oligonucleotides delivery using high hydrostatic pressurized lipoplex

2013 ◽  
Vol 1498 ◽  
pp. 3-8
Author(s):  
Tsuyoshi Kimura ◽  
Asami Sano ◽  
Kwangwoo Nam ◽  
Kazunari Akiyoshi ◽  
Yoshihiro Sasaki ◽  
...  
Keyword(s):  
Antisense Oligonucleotides ◽  
Transfection Efficiency ◽  
Cationic Liposome ◽  
Firefly Luciferase ◽  
Hek293 Cells ◽  
Luciferase Expression ◽  
Gene Suppression ◽  
Acid Therapy ◽  
Transfection Efficacy ◽  
Oligonucleotide Delivery

ABSTRACTCationic liposome (CL) is a promising vector for nucleic acid therapy. In the present study, we investigated the effect of high hydrostatic pressure (HHP) treatment to lipoplex on the lipoplex-based antisense oligodeoxynucleotides (AS-ODNs) delivery in order to improve the transfection efficacy of lipoplex. Cationic liposome consisting of DOTMA and DOPE was used. AS-ODNs were designed to inhibit the expression of firefly luciferase. The complexes of CL and AS-ODN were prepared at various C/A ratios and then pressurized hydrostatically at various atmospheres (∼10,000 atm) for 10 min (HHP treatment). After removal of pressure, the pressurized lipoplexes were used. The lipoplex with and without the HHP treatment was transferred into HeLa cells expressing firefly luciferase transiently. The luciferase activity using the HHP-treated lipoplex was decreased compared to that of the non-pressurized lipoplex. Also, for HEK293 cells expressing luciferase stably, the lipoplex with the HHP treatment could effectively suppress the luciferase expression. In order to elucidate relationship between the structure and the transfection efficiency of the HHP-treated lipoplex, the properties of the HHPtreated lipoplex were examined by various physicochemical analyses. The different physicochemical properties between the lipoplexes with and without HHP treatment were showed, suggesting that the nature of lipoplex was changed by the HHP treatment. We believe that this change of lipoplex properties by the HHP treatment affected the efficiency of gene suppression. This HHP treatment for lipoplex appears to be a promising contribution to gene and oligonucleotide delivery.

scholarly journals Rab11 and Lysotracker Markers Reveal Correlation between Endosomal Pathways and Transfection Efficiency of Surface-Functionalized Cationic Liposome–DNA Nanoparticles

2016 ◽  
Vol 120 (26) ◽  
pp. 6439-6453 ◽  
Author(s):  
Ramsey N. Majzoub ◽  
Emily Wonder ◽  
Kai K. Ewert ◽  
Venkata Ramana Kotamraju ◽  
Tambet Teesalu ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Cationic Liposome ◽  
Dna Nanoparticles

Enhancement of polyethylene glycol (PEG)-modified cationic liposome-mediated gene deliveries: effects on serum stability and transfection efficiency

2003 ◽  
Vol 55 (4) ◽  
pp. 453-460 ◽  
Author(s):  
Jin-Ki Kim ◽  
Sung-Hee Choi ◽  
Cheong-Ok Kim ◽  
Jeong-Sook Park ◽  
Woong-Shick Ahn ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Transfection Efficiency ◽  
Cationic Liposome ◽  
Serum Stability

Cationic liposome–DNA complexes: from liquid crystal science to gene delivery applications

2006 ◽  
Vol 364 (1847) ◽  
pp. 2573-2596 ◽  
Author(s):  
Cyrus R Safinya ◽  
Kai Ewert ◽  
Ayesha Ahmad ◽  
Heather M Evans ◽  
Uri Raviv ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Laser Scanning ◽  
Liquid Crystalline ◽  
Transfection Efficiency ◽  
Three Dimensional ◽  
Cationic Liposome ◽  
Laser Scanning Confocal Microscopy ◽  
Dna Complexes ◽  
Scanning Confocal Microscopy ◽  
Crystalline Nature

At present, there is an unprecedented level of interest in the properties and structures of complexes consisting of DNA mixed with oppositely charged cationic liposomes (CLs). The interest arises because the complexes mimic natural viruses as chemical carriers of DNA into cells in worldwide human gene therapy clinical trials. However, since our understanding of the mechanisms of action of CL–DNA complexes interacting with cells remains poor, significant additional insights and discoveries will be required before the development of efficient chemical carriers suitable for long-term therapeutic applications. Recent studies describe synchrotron X-ray diffraction, which has revealed the liquid crystalline nature of CL–DNA complexes, and three-dimensional laser-scanning confocal microscopy, which reveals CL–DNA pathways and interactions with cells. The importance of the liquid crystalline structures in biological function is revealed in the application of these modern techniques in combination with functional transfection efficiency measurements, which shows that the mechanism of gene release from complexes in the cell cytoplasm is dependent on their precise liquid crystalline nature and the physical and chemical parameters (for example, the membrane charge density) of the complexes. In §5 , we describe some recent new results aimed at developing bionanotube vectors for gene delivery.

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