Species of the genus Trametes represent one of the most efficient
lignin-degraders which can be attributed to a well developed ligninolytic
enzyme system. Current trends are screening of ability of new species to
produce these enzymes, as well as the optimization of conditions for their
overproduction. Therefore, the aim of the study was to evaluate the potential
of T. suaveolens to synthesize laccase and Mn-oxidizing peroxidases during
fermentation of the selected plant raw materials. Level of enzyme activities
was measured on 7, 10 and 14th day of submersion, as well as the solid-state
fermentation of wheat straw and oak sawdust in the presence of NH4NO3 in
previously determined optimal nitrogen concentration of 25 mM. The enzyme
activity was determined spectrophotometrically using ABTS and phenol red as
the substrates. The highest level of laccase activity (1087.1 U/L) was noted
after 7 days of wheat straw solid-state fermentation, while during the
submerged cultivation the production of the enzyme was not noted. Submerged
cultivation in oak sawdust-enriched medium was the optimal for activity of
Mn-dependent peroxidase (1767.7 U/L on day 14) and Mn-independent peroxidase
(1113.7 U/L on day 7). Introduction of T. suaveolens to produce ligninolytic
enzyme represented the base for further study, as well as the determination
of relation between enzyme activity and rate of lignin degradation. It could
lead to greater possibility of fungal species selection with high
delignification capacity, which could take participation in sustainable
production of food, feed, fibres, and energy, environmentally friendly
pollution prevention, and bioremediation.
Effect of Intercalation Structure of Organo-Modified Montmorillonite/Polylactic Acid on Wheat Straw Fiber/Polylactic Acid Composites