Evaluation of fungal epiphytes isolated from banana fruit surfaces for biocontrol of banana crown rot disease

Introduction: The banana is the world’s most popular fruit crop. A complex of fungal pathogen is responsible for crown rot diseases of banana. Aims: The present study was designed to detect and characterize the crown rot disease of post-harvest banana (Musa paradisiaca) and also develop an alternative quality improvement approach to improve banana shelf-life during storage period. Study Design: A simplest general factorial experiment that was designed to control crown rot disease of banana using different biological factors, including plant extract, antagonistic agents and commercial fungicide. Place and Duration of Study: Disease infected bananas were collected from Rajshahi city, Rajshahi, Bangladesh in 2017 and the experiment had been conducted from April 2017 to April 2018. Methodology: Different morphological, biochemical and molecular techniques were used to characterize and detect the liable fungi. Responsible fungi were subjected to antifungal activity screening test and in vitro antagonism test. Effect of carbendazim and kanamycin B against the mycelial growth of the isolates was determined by disc diffusion method. Quality parameters including disease incidence and severity, pH, TSS, TTA and AA of the treated banana were also analyzed after application of treatments in the packing stage through standard estimation techniques. Results: Two fungi, isolated from the infected portion were further identified as C. musae and L. theobromae. D. metel and A. sativum extract was better in inhibiting mycelial growth of all the test pathogen in culture. B. cereus and T. harzianum moved and attached to fungal isolates, affecting mycelial growth and A. sativum extract significantly affecting conidial germination on artificial medium. Satisfactory mycelia inhibitory effect was recorded from kanamycin B. Quality analysis after storage of banana showed minor measurable differences among treatments. Conclusion: Post-harvest application of A. sativum extract (Conc. 25% w/v) improve the overall quality of harvested banana fruits and reduced the disease incidence and severity of crown rot to a level significantly lower than in fungicide treated or control fruits.

Download Full-text

Identification of Genes Involved in the Response of Banana to Crown Rot Disease

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-01-10-0020 ◽

2011 ◽

Vol 24 (1) ◽

pp. 143-153 ◽

Cited By ~ 3

Author(s):

Ludivine Lassois ◽

Patrick Frettinger ◽

Luc de Lapeyre de Bellaire ◽

Philippe Lepoivre ◽

Haissam Jijakli

Keyword(s):

Gene Expression ◽

Molecular Mechanisms ◽

Cellulose Synthase ◽

Crown Rot ◽

Post Inoculation ◽

Banana Fruit ◽

High Susceptibility ◽

Pathogenesis Related Protein ◽

Host Pathogen ◽

Rot Disease

Variations in banana susceptibility to crown rot disease have been observed but the molecular mechanisms underlying these quantitative host–pathogen relationships are still unknown. This study was designed to compare gene expression between crowns of banana fruit showing a high susceptibility (S+) and crowns showing a low susceptibility (S–) to the disease. Comparisons were performed at two situation times: i) between crowns (S+ and S–) collected 1 h before inoculation and ii) between crowns (S+ and S–) collected 13 days after inoculation. Gene expression comparisons were performed with cDNA-amplified fragment length polymorphism (AFLP) and results were confirmed by real-time reverse-transcription polymerase chain reaction. Among genes identified as differentially expressed between S+ and S– crowns, two were involved in signal transduction, three in proteolytic machinery, two had similarity to pathogenesis-related protein 14, one to a CCR4-associated factor protein, and one to a cellulose synthase. Paradoxically, the overexpression of the cellulose synthase gene was associated with banana showing a high susceptibility in both pre- and post-inoculation situations. Finally, the cDNA-AFLP identified a gene that seems to be associated with the quantitative banana responses to crown rot disease; this gene encodes a dopamine-β-monooxygenase, which is involved in the catecholamine pathway. To our knowledge, this work is the first to address both pre- and post-infection gene expression with the same host–pathogen combination and distinct susceptibility levels.

Download Full-text

Hand position on the bunch and source-sink ratio influence the banana fruit susceptibility to crown rot disease

Annals of Applied Biology ◽

10.1111/j.1744-7348.2009.00381.x ◽

2010 ◽

Vol 156 (2) ◽

pp. 221-229 ◽

Cited By ~ 12

Author(s):

L. Lassois ◽

H. Bastiaanse ◽

M. Chillet ◽

A. Jullien ◽

M. H. Jijakli ◽

...

Keyword(s):

Crown Rot ◽

Hand Position ◽

Banana Fruit ◽

Rot Disease ◽

Source Sink

Download Full-text

Effects of cinnamon extract, chitosan coating, hot water treatment and their combinations on crown rot disease and quality of banana fruit

Postharvest Biology and Technology ◽

10.1016/j.postharvbio.2007.01.020 ◽

2007 ◽

Vol 45 (3) ◽

pp. 333-340 ◽

Cited By ~ 68

Author(s):

N. Kyu Kyu Win ◽

P. Jitareerat ◽

S. Kanlayanarat ◽

S. Sangchote

Keyword(s):

Water Treatment ◽

Hot Water ◽

Crown Rot ◽

Banana Fruit ◽

Hot Water Treatment ◽

Cinnamon Extract ◽

Chitosan Coating ◽

Rot Disease

Download Full-text

EFFICACY OF SACCHAROMYCES CEREVISIAE TO CONTROL CROWN ROT OF BANANA CAUSED BY FUSARIUM SEMITECTUM

Pakistan Journal of Phytopathology ◽

10.33866/phytopathol.030.01.0416 ◽

2018 ◽

Vol 30 (1) ◽

pp. 11

Author(s):

Muhammad Ather ◽

Muhammad Waris ◽

Muhammad Azhar ◽

Saeed Ahmed ◽

Masood Ahmed ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Plant Pathogens ◽

Crown Rot ◽

Fruit Rot ◽

Contact Method ◽

Disease Development ◽

Banana Fruit ◽

Yeast Saccharomyces Cerevisiae ◽

Fusarium Semitectum ◽

Rot Disease

Crown rot disease of Banana is adversely affecting the quantity and quality of the fruits for consumption and utilization. The bananas collected from the local markets of Hyderabad, Tando Allahyar and Quetta were found suffered with different types of rots such as crown rot, anthracnose, fruit rot and stem end rot. Among them crown rot of banana was found predominantly followed by anthracnose and other fruit rots. Incidence and severity varied with the markets and locations. Fusarium semitectum found predominately associated with the affected fruits showing crown rot symptoms. So yeast (Saccharomyces cerevisiae) were evaluated against F. semitectum under in-vitro conditions. S. cerevisiae also tested against crown rot disease development of banana fruit inoculated with F. semitectum. The addition of Saccharomyces cerevisiae in the culture medium has brought 93% reduction in the growth of F. semitectum. yeast were also tested against crown rot disease development on banana fruits by direct contact method. Yeast treatment brought more than 40% reduction in crown rot disease development as compared to the untreated fruits. The present study indicates that active yeast have the potential to manage harmful plant pathogens and thus could provide an alternate, reliable and eco-friendly control of crown rot disease.

Download Full-text

THE STOMATAL COMPLEX AND EPICUTICULAR CHARACTERISTICS OF CROWN ROT DISEASE OF AGAVE TEQUILANA WEBER (AGAVACEAE)

Acta Horticulturae ◽

10.17660/actahortic.2003.618.51 ◽

2003 ◽

pp. 427-433 ◽

Cited By ~ 1

Author(s):

R.E.M. Hernandéz-Valencia ◽

R. López-Franco ◽

J. Ruíz-Ordoñez ◽

H. Ramirez-Rodriguez ◽

A. Benavides-Mendoza

Keyword(s):

Crown Rot ◽

Agave Tequilana ◽

Stomatal Complex ◽

Rot Disease

Download Full-text

Antagonistic effectiveness of some bacteria against Fusarium graminearum causing crown rot disease on wheat (Triticum aestivum)

Diyala Agricultural Sciences Journal ◽

10.52951/dasj.21130107 ◽

2021 ◽

Vol 13 (1) ◽

pp. 69-80

Author(s):

Majida Hadi Mahdi Alsaady ◽

Hussein Ali Salim ◽

Rakib A. Al-ani ◽

Hadi M. Aboud ◽

Jamal Talib M Al Roubaie

Keyword(s):

Fusarium Graminearum ◽

Culture Method ◽

Dry Weight ◽

Antagonistic Effect ◽

Crown Rot ◽

Control Treatment ◽

Enzyme Analysis ◽

Dual Culture ◽

Rot Disease

In this study, the antagonistic effect of five bacteria genera namely Bacillus, Pseudomonas, Azotobacter, Azospirillum, and Streptomyces isolated from rhizosphere of wheat were evaluated against Fusarium graminearum as potential biocontrol agents in vitro. F. graminearum was molecularly diagnosed using the Polymerase chain reaction (PCR) technique. Each bacteria were tested for the production of catalase enzyme, oxidase enzyme, analysis of starch, analyze of gelatin, and the motility, where Azotobacter, Azospirillum, and Bacillus subtilis were positive for all tested. Fungal inhibition tests were performed by using the dual culture method and agar well diffusion technique. Among them, Streptomyces and Azospirillum exhibited potent inhibition to the growth of F. graminearum (72.14% and 66.42%) respectively, followed by B.pumillus, P.fluorescens, B. subtilis and Azotobacter ( 58.28%, 43.23%, 39.71% and 35.71%) respectively as compared with the control treatment (0.0%).The dry weight of the fungus biomass was decreased with bacteria P. fluorescens, Streptomyces sp, Azotobacter sp, Azospirillum sp, B. subtilis, and B. pumillus which reached (0.114, 0.103, 0.147, 0.101, 0.143, and 0.107 g) respectively compared to the control treatment that was 0. 665 g.

Download Full-text

Disease responses of hexaploid spring wheat (Triticum aestivum) culms exhibiting premature senescence (dead heads) associated with Fusarium pseudograminearum crown rot

10.1101/2020.05.13.094979 ◽

2020 ◽

Author(s):

N. L. Knight ◽

B. Macdonald ◽

C. Percy ◽

M. W. Sutherland

Keyword(s):

Triticum Aestivum ◽

Spring Wheat ◽

Crown Rot ◽

Fusarium Pseudograminearum ◽

Disease States ◽

South Wales ◽

Commercial Cultivars ◽

Dry Conditions ◽

Timing Of Initiation ◽

Rot Disease

AbstractHexaploid spring wheat (Triticum aestivum) may exhibit significant crown rot disease responses to infection by Fusarium pseudograminearum, with a range of susceptibility levels available in commercial cultivars. Dry conditions during grain-fill may lead to the expression of prematurely senescing culms, which typically fail to set grain. Assessment of hexaploid spring wheat plants exhibiting both non-senescent and prematurely senescent culms was performed using visual discolouration, Fusarium pseudograminearum biomass, vascular colonisation and quantification of wheat DNA in culm sections sampled at three different heights above the crown and at the peduncle. A comparison of these parameters at four time points from milk development, when senescent culms are first observed, to maturity was conducted. Samples from six commercial cultivars were collected in 2014 from Narrabri and Tamworth, New South Wales and Wellcamp, Queensland. Prematurely senescent culms exhibited greater visual discolouration, Fusarium pseudograminearum biomass and vascular colonisation than non-senescent culms in each cultivar. Colonisation of xylem and phloem tissue was extensive in the basal portions of prematurely senescent culms (36 to 99%), and suggests significant impacts on water and nutrient movement during crown rot disease. Maturation coincided with significant changes in Fusarium pseudograminearum biomass and vascular colonisation. Wheat DNA content varied among cultivars, culm conditions, culm sections and sampling times. The variation in the severity of disease states between culms of the same plant suggests that the timing of initiation of infection in individual culms may vary.

Download Full-text