Molecular diagnosis of malaria in the field: development of a novel 1-step nucleic acid lateral flow immunoassay for the detection of all 4 human Plasmodium spp. and its evaluation in Mbita, Kenya

2008 ◽  
Vol 61 (4) ◽  
pp. 421-427 ◽  
Author(s):  
Petra F. Mens ◽  
Aart van Amerongen ◽  
Patrick Sawa ◽  
Piet A. Kager ◽  
Henk D.F.H. Schallig
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnosis ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Field Development ◽  
Human Plasmodium

scholarly journals Plasmodium Detection and Differentiation by Direct-on-Blood PCR Nucleic Acid Lateral Flow Immunoassay

2018 ◽  
Vol 20 (1) ◽  
pp. 78-86 ◽  
Author(s):  
Johanna M. Roth ◽  
Laura de Bes ◽  
Patrick Sawa ◽  
George Omweri ◽  
Victor Osoti ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

Development of a nucleic acid lateral flow immunoassay (NALFIA) for reliable, simple and rapid detection of the methicillin resistance genes mecA and mecC

2017 ◽  
Vol 200 ◽  
pp. 101-106 ◽  
Author(s):  
Constanze Seidel ◽  
Sonja Peters ◽  
Erik Eschbach ◽  
Andrea T. Feßler ◽  
Boris Oberheitmann ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Resistance Genes ◽  
Rapid Detection ◽  
Methicillin Resistance ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

scholarly journals Direct Blood PCR in Combination with Nucleic Acid Lateral Flow Immunoassay for Detection of Plasmodium Species in Settings Where Malaria Is Endemic

2012 ◽  
Vol 50 (11) ◽  
pp. 3520-3525 ◽  
Author(s):  
P. F. Mens ◽  
H. M. de Bes ◽  
P. Sondo ◽  
N. Laochan ◽  
L. Keereecharoen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Plasmodium Species ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

scholarly journals Development of nucleic-acid lateral flow immunoassay for rapid and accurate detection of Chikungunya virus in Indonesia

2021 ◽  
Author(s):  
Mandala Ajie ◽  
Dyshelly Nurkartika Pascapurnama ◽  
Susantina Prodjosoewojo ◽  
Shinta Kusumawardani ◽  
Hofiya Djauhari ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Chikungunya Virus ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Accurate Detection

scholarly journals Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food

2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S350-S353 ◽  
Author(s):  
M. Blažková ◽  
M. Koets ◽  
J. H Wichers ◽  
A. van Amerongen ◽  
L. Fukal ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Pathogenic Bacteria ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Carbon Particles ◽  
Microbiological Methods ◽  
Biological Principle ◽  
Primer Sets ◽  
Food Bacteria

Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the other with digoxigenin or fluorescein, respectively, was performed. The PCR solution and carbon particles conjugated with avidin are directly added to the nitrocellulose membrane with two test lines of immobilised antibodies specific for digoxigenin and fluorescein. The appearance of a black line indicates the presence of specific amplicon. We would like to present the NALFIA for the simultaneous detection of L. monocytogenes in particular and the genus Listeria in general, in food. Bacteria from the genus Listeria frequently contaminate a large variety of foods. Occurrence of Listeria strains in food may indicate errors in good hygienic and manufacturing practice, only L. monocytogenes is a significant human and animal pathogen responsible for the serious illness listeriosis. Conventional microbiological methods for L. monocytogenes detection are laborious and take several days to achieve a confirmed identification.

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