Application of hypertonic Sabouraud glucose agar for differentiation of Candida dubliniensis from Candida albicans

Abstract Objective The objectives of this study were to investigate the antifungal susceptibility and genetic diversity of Candida albicans isolated from HIV+ patients with oropharyngeal candidiasis. A total of 50 C. albicans isolates were cultured on Sabouraud glucose agar containing chloramophenicol. The antifungal susceptibility of the isolates against fluconazole, clotrimazole, nystatin, amphotericin B, ketoconazole and flucytosine was assessed using disc diffusion method. The genetic diversity of C. albicans isolates was determined using random amplified polymorphic DNA marker. Results The inhibition zones ranged from 4 ± 1.8 to 40 ± 3.8 mm for fluconazole, 7 ± 1.0 to 37 ± 1.8 mm for ketoconazole, 14 ± 0.8 to24 ± 0.8 mm for amphotericin B, 25 ± 0.0 to 33 ± 0.0 mm for nystatin and 7 ± 4.2 to 40 ± 0.0 mm for clotrimazole. At 90% similarity, three distinct groups were observed. The smallest cluster composed of 3 isolates, whereas the largest one composed of 17 isolates. 32% (16/50), 28% (14/50) and 14% (7/50) were resistant to fluconazole, ketoconazole and clotrimazole, respectively.

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UJI AKTIVITAS ANTI JAMUR EKSTRAK INFUSA DAUN SIRSAK (Annona muricata L.) TERHADAP Candida albicans

Jurnal Borneo Cendekia ◽

10.54411/jbc.v1i1.187 ◽

2017 ◽

Vol 1 (1) ◽

pp. 83-92

Author(s):

MIFTACHUL SOBIRIN

Keyword(s):

Candida Albicans ◽

Annona Muricata ◽

Glucose Agar ◽

Sabouraud Glucose Agar

Kandidiasis adalah suatu penyakit jamur, yang bersifat akut atau sub akut disebabkan oleh Candida albicans dapat menyerang daerah kulit, mulut, selaput mukosa vagina, kuku, bronki atau paru-paru. Penyakit ini ditemukan di seluruh dunia dan dapat menyerang semua umur baik laki-laki maupun perempuan. Pengetahuan tentang obat merupakan warisan budaya bangsa salah satunya jenis tanaman berkhasiat obat daun sirsak terhadap Candida albicans. Penelitan dengan metode true eksperimental, Populasi daun sirsak (Annona Muricata L.), diperoleh dari daerah Pasir Panjang, Pangkalan Bun, Kal-Teng. Jamur uji yang digunakan dalam penelitian ini adalah jamur Candida albicans. Medium yang digunakan dalam penelitian ini adalah medium SGA (Sabouraud Glucose Agar) + khloramfenikol 75 ppm dan medium SGC (Sabouraud Glucose Cair). Daun sirsak yang digunakan adalah daun yang berasal dari tanaman sirsak dewasa. Pengujian anti jamur dilakukan dengan metode dilusi atau seri pengenceran dengan interval pengenceran dua kali, menggunakan 12 tabung reaksi, tiap tabung dimasukkan sebanyak 0,5 ml SGC secara aseptis. Semua tabung diinkubasi selama 5-7 hari pada suhu kamar, kemudian diamati adanya pertumbuhan (kekeruhan) dengan cara membandingkan dengan kontrol negatif dan kontrol positif. Hasil Penelitian yaitu Infusa daun sirsak mempunyai aktivitas untuk menghambat dan membunuh pertumbuhan jamur Candida albicans yang menunjukkan KHM dan KBM pada konsentrasi 12,5%. Kata Kunci : Aktivitas, Infusa, Daun Sirsak, Candida albicans.

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Simple, Inexpensive, Reliable Method for Differentiation of Candida dubliniensis fromCandida albicans

Journal of Clinical Microbiology ◽

10.1128/jcm.36.7.2093-2095.1998 ◽

1998 ◽

Vol 36 (7) ◽

pp. 2093-2095 ◽

Cited By ~ 124

Author(s):

Emmanuelle Pinjon ◽

Derek Sullivan ◽

Ira Salkin ◽

Diarmuid Shanley ◽

David Coleman

Keyword(s):

Reliable Method ◽

Candida Dubliniensis ◽

Pathogenic Species ◽

Inexpensive Method ◽

Yeast Peptone Dextrose ◽

Clinical Mycology ◽

Glucose Agar ◽

Sabouraud Glucose Agar ◽

Germ Tubes ◽

Mycology Laboratory

Candida dubliniensis is a recently described pathogenic species which shares many phenotypic features with Candida albicans, including the ability to form germ tubes and chlamydospores. These similarities have caused significant problems in the identification of C. dubliniensis by the average clinical mycology laboratory. To facilitate the differentiation of these species, we investigated the growth of 120 isolates of C. dubliniensis and 98 C. albicans isolates at 42 and 45°C on Emmons’ modified Sabouraud glucose agar (SGA) and 10 isolates of each species in yeast-peptone-dextrose broth. None of theC. dubliniensis isolates grew on the agar or in the broth medium at 45°C, while 11 isolates were capable of growing on SGA at 42°C. In contrast, all of the C. albicans isolates but one grew at 45°C on or in either medium. These reproducible results clearly demonstrate that the incubation of isolates suspected to beC. dubliniensis or C. albicans at 45°C provides a simple, reliable, and inexpensive method for the differentiation of the two species.

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Genetic Diversity and Antifungal Susceptibility of Candida Albicans Strains Isolated from Lranian HIV+ Patients With Oral Candidiasis

10.21203/rs.3.rs-127737/v1 ◽

2020 ◽

Author(s):

Iradj Ashrafi Tamai ◽

Babak Pakbin ◽

Bahar Nayeri Fasaei

Keyword(s):

Genetic Diversity ◽

Candida Albicans ◽

Amphotericin B ◽

Random Amplified Polymorphic Dna ◽

Oral Candidiasis ◽

Antifungal Susceptibility ◽

Diffusion Method ◽

Glucose Agar ◽

Sabouraud Glucose Agar ◽

Inhibition Zones

Abstract Objective: The objectives of this study were to investigate the antifungal susceptibility and genetic diversity of oral Candida albicans strains isolated from HIV+ patients with oropharyngeal candidiasis. A total of 50 C. albicans isolates were cultured on Sabouraud glucose agar containing chloramohenicol. The antifungal susceptibility of C. albicans against fluconazole, clotrimazole, nystatin, amphotericin B, ketoconazole and flucytosine was assessed using disc diffusion method. The genetic diversity of different C. albicans strains was determined using random amplified polymorphic DNA technique. Results: The inhibition zones ranged from 4±1.8 to 40±3.8 mm for fluconazole, 7±1.0 to 37±1.8 mm for ketoconazole, 14±0.8 to24±0.8 mm for amphotericin B, 25±0.0 to 33±0.0 mm for nystatin and 7±4.2 to 40±0.0 mm for clotrimazole. At 90% similarity, three distinct groups were observed. The smallest cluster composed of 3 of 50 C. albicans isolates, whereas the largest cluster composed of 17 of 50 isolates. Of 50 C. albicans isolates, 32%, 28% and 14% were resistant to fluconazole, ketoconazole and clotrimazole, respectively. There were no significant differences among antifungal susceptibility of different C. albicans strains from three genotype clusters.

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UJI AKTIVITAS ANTI JAMUR EKSTRAK INFUSA DAUN SIRSAK (Annona muricata L.) TERHADAP Candida albicans

Jurnal Medikes (Media Informasi Kesehatan) ◽

10.36743/medikes.v6i2.181 ◽

2019 ◽

Vol 6 (2) ◽

pp. 167-176

Author(s):

Risa Wahyuningsih ◽

Kartinah Wiryosoendjoyo

Keyword(s):

Candida Albicans ◽

Annona Muricata ◽

Post Test ◽

Glucose Agar ◽

Sabouraud Glucose Agar

Kandidiasis banyak menyerang masyarakat di daerah tropis seperti Indonesia, hal ini disebabkan Indonesia memiliki curah hujan yang tinggi dan kelembapan yang tinggi sehingga pertumbuhan jamur menjadi sangat baik. Kandidiasis adalah suatu penyakit jamur, yang bersifat akut atau sub akut disebabkan oleh Candida albicans. Candida albicans merupakan salah satu jamur patogen penyebab keputihan. Sejak jaman dahulu masyarakat Indonesia telah mengenal dan menggunakan tanaman berkhasiat obat sebagai penanggulangan masalah kesehatan. Sirsak (Annona muricata L.) merupakan salah satu tanaman obat tradisional yang mengandung senyawa kimia yaitu tanin, fitosterol, kalsium oksalat dan alkaloid murisine yang dapat digunakan sebagai anti jamur. Penelitian ini bertujuan untuk mengetahui aktivitas infusa daun sirsak dalam menghambat atau membunuh pertumbuhan jamur Candida albicans. Penelitian dilakukan di Laboratorium Mikologi Poltekkes Kemenkes Banjarmasin. Penelitian bersifat experimental dengan desain post-test only. Ekstraksi daun sirsak menggunakan metode infusa. Metode yang digunakan adalah metode dilusi. Aktivitas anti jamur diamati dengan melihat kejernihan dan kekeruhan pada 50%; 25%; 12,5%; 6,25%; 3,13%; 1,56%; 0,79%; 0,40%; 0,20% dan 0,10%. Medium yang digunakan adalah medium SGA (Sabouraud Glucose Agar) yang ditambahkan Antibiotik Khloramfenikol 75 ppm dan medium SGC (Sabouraud Glucose Cair). Infusa daun sirsak mempunyai aktivitas menghambat dan membunuh pertumbuhan jamur Candida albicans. Infusa daun sirsak menunjukkan KHM dan KBM pada konsentrasi 12,5%.

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The importance of strain variation in virulence of Candida dubliniensis and Candida albicans: results of a blinded histopathological study of invasive candidiasis

Clinical Microbiology and Infection ◽

10.1111/j.1469-0691.2009.02840.x ◽

2009 ◽

Vol 15 (6) ◽

pp. 576-585 ◽

Cited By ~ 26

Author(s):

L.R. Ásmundsdóttir ◽

H. Erlendsdóttir ◽

B.A. Agnarsson ◽

M. Gottfredsson

Keyword(s):

Candida Albicans ◽

Invasive Candidiasis ◽

Candida Dubliniensis ◽

Histopathological Study ◽

Strain Variation

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Evaluation of various phenotypic methods for differentiation of Candida dubliniensis from Candida albicans

Journal of Microbiological Methods ◽

10.1016/j.mimet.2021.106400 ◽

2021 ◽

pp. 106400

Author(s):

Jan Abiroo ◽

Gulnaz Bashir ◽

Insha Altaf ◽

A. Fomda Bashir ◽

Sabiya Hamid ◽

...

Keyword(s):

Candida Albicans ◽

Candida Dubliniensis ◽

Phenotypic Methods

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Genotyping of Candida albicans and Candida dubliniensis by 25S rDNA analysis shows association with virulence attributes in oral candidiasis

Archives of Oral Biology ◽

10.1016/j.archoralbio.2018.10.006 ◽

2019 ◽

Vol 97 ◽

pp. 18-24 ◽

Cited By ~ 2

Author(s):

Pornpen Tantivitayakul ◽

Naruemon Panpradit ◽

Thaniya Maudcheingka ◽

Arthit Klaophimai ◽

Jinthana Lapirattanakul

Keyword(s):

Candida Albicans ◽

Oral Candidiasis ◽

Candida Dubliniensis ◽

25S Rdna ◽

Rdna Analysis

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Molecular characterization of Candida dubliniensis and Candida albicans in the oral cavity of drug abusers using duplex polymerase chain reaction

Current Medical Mycology ◽

10.18502/cmm.4.1.29 ◽

2018 ◽

Vol 4 (1) ◽

Cited By ~ 1

Author(s):

Parastoo Hassani Abharian ◽

Parvin Dehghan ◽

Peyman Hassani Abharian ◽

Sepideh Tolouei

Keyword(s):

Polymerase Chain Reaction ◽

Candida Albicans ◽

Oral Cavity ◽

Candida Species ◽

Species Complex ◽

Candida Dubliniensis ◽

Drug Abusers ◽

Chain Reaction ◽

Polymerase Chain ◽

Duplex Polymerase Chain Reaction

Background and Purpose: Candida dubliniensis is closely related to the most pathogenic and prevalent yeast, namely C. albicans. Candida species can opportunistically overgrow in vulnerable individuals and cause a variety of diseases. The current study aimed to identify and isolate C. dubliniensis species present in the Candida albicans species complex identified in the oral cavity of drug abusers. Materials and Methods: This study was conducted on 53 strains of C. albicans species complex, isolated from the oral mucosa of drug abusers in Isfahan, Iran. DNA extraction was accomplished through boiling procedure. Duplex polymerase chain reaction (PCR) was performed to amplify ITS1-5.8S-ITS2 region using four specific primers. Fungal species were identified based on the difference in the size of the bands created in the Agarose gel. Results: Out of the 53 isolates under study, 30 (56.6%) and 14 (26.4%) samples were identified as C. albicans and C. dubliniensis, respectively. In the remaining 9 samples (17%), both types of Candida species were confirmed. Conclusion: The findings of the present study revealed the presence of a noticeable amount of C. dubliniensis in the oral cavity of drug abusers. Therefore, the probable presence of this fungus should be considered during the examination of oral infection among this group. To date, no research has directly investigated this issue in Iran.

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