scholarly journals UJI AKTIVITAS ANTI JAMUR EKSTRAK INFUSA DAUN SIRSAK (Annona muricata L.) TERHADAP Candida albicans

2017 ◽  
Vol 1 (1) ◽  
pp. 83-92
Author(s):  
MIFTACHUL SOBIRIN
Keyword(s):  
Candida Albicans ◽  
Annona Muricata ◽  
Glucose Agar ◽  
Sabouraud Glucose Agar

Kandidiasis adalah suatu penyakit jamur, yang bersifat akut atau sub akut disebabkan oleh Candida albicans dapat menyerang daerah kulit, mulut, selaput mukosa vagina,  kuku, bronki atau paru-paru. Penyakit ini ditemukan di seluruh dunia dan dapat menyerang semua umur baik laki-laki maupun perempuan. Pengetahuan tentang obat merupakan warisan budaya bangsa salah satunya jenis tanaman berkhasiat obat daun sirsak terhadap Candida albicans. Penelitan dengan metode true eksperimental, Populasi daun sirsak (Annona Muricata L.), diperoleh dari daerah Pasir Panjang, Pangkalan Bun, Kal-Teng. Jamur uji yang digunakan dalam penelitian ini adalah jamur Candida albicans. Medium yang digunakan dalam penelitian ini adalah medium SGA (Sabouraud Glucose Agar) + khloramfenikol 75 ppm dan medium SGC (Sabouraud Glucose Cair). Daun sirsak yang digunakan adalah daun yang berasal dari tanaman sirsak dewasa. Pengujian anti jamur dilakukan dengan metode dilusi atau seri pengenceran dengan interval pengenceran dua kali, menggunakan 12 tabung reaksi, tiap tabung dimasukkan sebanyak 0,5 ml SGC secara aseptis. Semua tabung diinkubasi selama 5-7 hari pada suhu kamar, kemudian diamati adanya pertumbuhan (kekeruhan) dengan cara membandingkan dengan kontrol negatif dan kontrol positif. Hasil Penelitian yaitu Infusa daun sirsak mempunyai aktivitas untuk menghambat dan membunuh pertumbuhan jamur Candida albicans yang menunjukkan KHM dan KBM pada konsentrasi 12,5%. Kata Kunci : Aktivitas, Infusa, Daun Sirsak, Candida albicans.

scholarly journals UJI AKTIVITAS ANTI JAMUR EKSTRAK INFUSA DAUN SIRSAK (Annona muricata L.) TERHADAP Candida albicans

2019 ◽  
Vol 6 (2) ◽  
pp. 167-176
Author(s):  
Risa Wahyuningsih ◽  
Kartinah Wiryosoendjoyo
Keyword(s):  
Candida Albicans ◽  
Annona Muricata ◽  
Post Test ◽  
Glucose Agar ◽  
Sabouraud Glucose Agar

Kandidiasis banyak menyerang masyarakat di daerah tropis seperti Indonesia, hal ini disebabkan Indonesia memiliki curah hujan yang tinggi dan kelembapan yang tinggi sehingga pertumbuhan jamur menjadi sangat baik. Kandidiasis adalah suatu penyakit jamur, yang bersifat akut atau sub akut disebabkan oleh Candida albicans. Candida albicans merupakan salah satu jamur patogen penyebab keputihan. Sejak jaman dahulu masyarakat Indonesia telah mengenal dan menggunakan tanaman berkhasiat obat sebagai penanggulangan masalah kesehatan. Sirsak (Annona muricata L.) merupakan salah satu tanaman obat tradisional yang mengandung senyawa kimia yaitu tanin, fitosterol, kalsium oksalat dan alkaloid murisine yang  dapat digunakan sebagai anti jamur. Penelitian ini bertujuan untuk mengetahui aktivitas infusa daun sirsak dalam menghambat atau membunuh pertumbuhan jamur Candida albicans. Penelitian dilakukan di Laboratorium Mikologi Poltekkes Kemenkes Banjarmasin. Penelitian bersifat experimental dengan desain post-test only. Ekstraksi daun sirsak menggunakan metode infusa. Metode yang digunakan adalah metode dilusi. Aktivitas anti jamur diamati dengan melihat kejernihan dan kekeruhan pada 50%; 25%; 12,5%; 6,25%; 3,13%; 1,56%; 0,79%; 0,40%; 0,20% dan 0,10%. Medium yang digunakan adalah medium SGA (Sabouraud Glucose Agar) yang ditambahkan Antibiotik Khloramfenikol 75 ppm dan medium SGC (Sabouraud Glucose Cair). Infusa daun sirsak mempunyai aktivitas menghambat dan membunuh pertumbuhan jamur Candida albicans. Infusa daun sirsak menunjukkan KHM dan KBM pada konsentrasi 12,5%.

Application of hypertonic Sabouraud glucose agar for differentiation of Candida dubliniensis from Candida albicans

2011 ◽  
Vol 69 (4) ◽  
pp. 440-442 ◽  
Author(s):  
Anuradha Chowdhary ◽  
Harbans S. Randhawa ◽  
Tusharantak Kowshik ◽  
Shallu Kathuria ◽  
Pradip Roy ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Candida Dubliniensis ◽  
Glucose Agar ◽  
Sabouraud Glucose Agar

scholarly journals Genetic diversity and antifungal susceptibility of Candida albicans isolates from Iranian HIV-infected patients with oral candidiasis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Iradj Ashrafi Tamai ◽  
Babak Pakbin ◽  
Bahar Nayeri Fasaei
Keyword(s):  
Genetic Diversity ◽  
Candida Albicans ◽  
Amphotericin B ◽  
Random Amplified Polymorphic Dna ◽  
Oral Candidiasis ◽  
Antifungal Susceptibility ◽  
Diffusion Method ◽  
Glucose Agar ◽  
Sabouraud Glucose Agar ◽  
Inhibition Zones

Abstract Objective The objectives of this study were to investigate the antifungal susceptibility and genetic diversity of Candida albicans isolated from HIV+ patients with oropharyngeal candidiasis. A total of 50 C. albicans isolates were cultured on Sabouraud glucose agar containing chloramophenicol. The antifungal susceptibility of the isolates against fluconazole, clotrimazole, nystatin, amphotericin B, ketoconazole and flucytosine was assessed using disc diffusion method. The genetic diversity of C. albicans isolates was determined using random amplified polymorphic DNA marker. Results The inhibition zones ranged from 4 ± 1.8 to 40 ± 3.8 mm for fluconazole, 7 ± 1.0 to 37 ± 1.8 mm for ketoconazole, 14 ± 0.8 to24 ± 0.8 mm for amphotericin B, 25 ± 0.0 to 33 ± 0.0 mm for nystatin and 7 ± 4.2 to 40 ± 0.0 mm for clotrimazole. At 90% similarity, three distinct groups were observed. The smallest cluster composed of 3 isolates, whereas the largest one composed of 17 isolates. 32% (16/50), 28% (14/50) and 14% (7/50) were resistant to fluconazole, ketoconazole and clotrimazole, respectively.

scholarly journals Genetic Diversity and Antifungal Susceptibility of Candida Albicans Strains Isolated from Lranian HIV+ Patients With Oral Candidiasis

2020 ◽  
Author(s):  
Iradj Ashrafi Tamai ◽  
Babak Pakbin ◽  
Bahar Nayeri Fasaei
Keyword(s):  
Genetic Diversity ◽  
Candida Albicans ◽  
Amphotericin B ◽  
Random Amplified Polymorphic Dna ◽  
Oral Candidiasis ◽  
Antifungal Susceptibility ◽  
Diffusion Method ◽  
Glucose Agar ◽  
Sabouraud Glucose Agar ◽  
Inhibition Zones

Abstract Objective: The objectives of this study were to investigate the antifungal susceptibility and genetic diversity of oral Candida albicans strains isolated from HIV+ patients with oropharyngeal candidiasis. A total of 50 C. albicans isolates were cultured on Sabouraud glucose agar containing chloramohenicol. The antifungal susceptibility of C. albicans against fluconazole, clotrimazole, nystatin, amphotericin B, ketoconazole and flucytosine was assessed using disc diffusion method. The genetic diversity of different C. albicans strains was determined using random amplified polymorphic DNA technique. Results: The inhibition zones ranged from 4±1.8 to 40±3.8 mm for fluconazole, 7±1.0 to 37±1.8 mm for ketoconazole, 14±0.8 to24±0.8 mm for amphotericin B, 25±0.0 to 33±0.0 mm for nystatin and 7±4.2 to 40±0.0 mm for clotrimazole. At 90% similarity, three distinct groups were observed. The smallest cluster composed of 3 of 50 C. albicans isolates, whereas the largest cluster composed of 17 of 50 isolates. Of 50 C. albicans isolates, 32%, 28% and 14% were resistant to fluconazole, ketoconazole and clotrimazole, respectively. There were no significant differences among antifungal susceptibility of different C. albicans strains from three genotype clusters.

scholarly journals Majocchi's granuloma of the vulva: A case report

2020 ◽  
Vol 49 (4) ◽  
pp. 47-52
Author(s):  
Milan Bjekić ◽  
Danijela Pecarski
Keyword(s):  
Case Report ◽  
Potassium Hydroxide ◽  
Trichophyton Rubrum ◽  
Subcutaneous Tissue ◽  
Hair Follicles ◽  
Glucose Agar ◽  
Nodular Lesions ◽  
Sabouraud Glucose Agar ◽  
Majocchi’S Granuloma ◽  
Immunocompromised Hosts

Introduction/Aim: Majocchi's granuloma is an infrequent deep-seated fungal infection where pathogen invades hair follicles, entering the dermal and subcutaneous tissue, thus forming granulomatous dermal and/or hypodermal changes. There are two clinical types: the first one is common in healthy individuals characterized by superficial perifollicular papular infection, and the second is followed by the deep subcutaneous nodules usually reported among immunocompromised hosts. This infection is usually caused by Trichophyton rubrum. The aim of this paper is to show the rare localization of this disease in the area of the vulva. Case report: We present a 20-year-old immunocompetent woman with multiple papules, nodules, and pustules on the hairy part of the vulva. Potassium hydroxide preparations of skin scrapings were negative and culture performed on Sabouraud glucose agar revealed Trichophyton rubrum. The patient was treated with the oral systemic antifungal therapy for four weeks and all lesions resolved. Conclusion: Majocchi's granuloma should not be overlooked in patients with papular and nodular lesions in the vulvar region.

scholarly journals Konsentrasiekstrakdaunsirsak(Annonamuricata)yangmenghambatpertumbuhan Candida albicans pada lempeng resin akrilik polimerisasi panas Concentration of soursop (Annona muricata)leaf extract in inhibiting Candida albicans on heat cure acrylic resin plate

2013 ◽  
Vol 12 (3) ◽  
pp. 159
Author(s):  
Bahruddin Thalib ◽  
Herawati Hasan
Keyword(s):  
Candida Albicans ◽  
Test Data ◽  
Leaf Extract ◽  
Agar Medium ◽  
Acrylic Resin ◽  
Annona Muricata ◽  
Heat Curing ◽  
Heat Cure ◽  
Seeds And Fruits

Soursops (Annona muricata)are plants that have flower and sweet fruit. These plants have been used since long fortraditional medicine, especially the leaves, seeds, and fruits. This study aimed to determine the effect of concentrationof soursop leaf extract to the growth of Candida albicans on heat curing acrylic resin plate. The study begins with themanufacture of soursop leaf extract concentrations of 5%, 15%, 25%, 35%, and 45%, dilution of the extract, thepurification of C.albicans, growing C.albicans on saboraud dextrose agar medium, and minimum inhibitoryconcentration test. Data were analyzed by ANOVA followed by LSD test using SPSS 16.0. The results showed asignificant difference in the average number of C.albicans that grows between each concentration soursop leaf extract(α<0.05) and soursop leaf extract at a concentration of 45% is the most effective in inhibiting the growth of C.albicans.It was concluded that the higher the concentration of soursop leaf extract, the more effective in inhibiting the growth ofC.albicans.

scholarly journals Membrane Filtration Test for Rapid Presumptive Differentiation of Four Candida Species

1999 ◽  
Vol 37 (5) ◽  
pp. 1498-1502 ◽  
Author(s):  
T. G. Bauters ◽  
R. Peleman ◽  
M. Moerman ◽  
H. Vermeersch ◽  
D. de Looze ◽  
...  
Keyword(s):  
Membrane Filtration ◽  
Uv Light ◽  
Membrane Filter ◽  
False Negative ◽  
Single Species ◽  
Step Test ◽  
Clinical Samples ◽  
Glucose Agar ◽  
Sabouraud Glucose Agar ◽  
Multiple Species

A rapid enzymatic two-step test for the presumptive differentiation of four Candida species commonly occurring in various clinical samples is described. The technique involves membrane filtration of a liquid sample, followed by preincubation of the membrane filter on Sabouraud glucose agar supplemented with ticarcillin-clavulanic acid to yield microcolonies. In a separate assay step, parts of the filter are placed on absorbent pads impregnated with fluorogenic 4-methylumbelliferyl (4-MU) enzyme substrates (4-MU-N-acetyl-β-d-galactosaminide, 4-MU-phosphate, 4-MU-pyrophosphate, and 4-MU-β-d-galactoside) in combination with 0.1% digitonin acting as a membrane permeabilizer. The membrane filter in contact with the assay medium is incubated to allow cleavage of the enzyme substrate, resulting in fluorescent microcolonies under long-wavelength UV light. This approach, tested on 301 clinical samples, is able to presumptively differentiate C. albicans, C. glabrata, C. krusei, and C. tropicalisand to distinguish them from other Candida spp. in about 9 to 11 h. Overall agreement with the conventional methods of 94.4% (one Candida species present in the sample) to 83.8% (multiple Candida spp. present) was obtained. The false-negative rates with reference to identification by traditional methods were 1.3% (single species) and 3.8% (multiple species).

scholarly journals Ação antifúngica de extratos e frações de Annona muricata L. sobre Candida spp.

2021 ◽  
Vol 10 (5) ◽  
pp. e28010514938
Author(s):  
Kellyane Karen Ferreira Aguiar Cesar ◽  
Anny Karoline Rodrigues Batista ◽  
Luciana Rocha Paula ◽  
Reginara Teixeira da Silva ◽  
Francisco Laurindo da Silva
Keyword(s):  
Candida Albicans ◽  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Candida Krusei ◽  
Annona Muricata ◽  
Candida Spp

Devido ao amplo potencial medicinal de Annona muricata, as pesquisas relacionadas às suas aplicações em diferentes áreas ligadas a saúde são de fundamental importância, principalmente no que se refere a microrganismos infecciosos com tendências a desenvolver mecanismos de resistência, que é o caso de algumas espécies do gênero Candida. Nesse sentido, a pesquisa tem como principal objetivo verificar a ação antifúngica de extratos metanólicos de Annona muricata sobre Candida albicans, Candida parapsilosis, Candida krusei e  Candida glabrata. Os órgãos utilizados para obtenção dos extratos metanólicos foram a folha e o fruto da Annona muricata. Para a realização da pesquisa, foram utilizadas cepas ATCC, que foram reativadas e cultivadas em Ágar Saboraud Dextrose. A determinação da atividade antifúngica dos extratos foi realizada pela técnica da difusão em ágar em poços. Na análise dos resultados, os valores de mensuração dos diâmetros dos halos indicaram uma ação mais significativa nos testes realizados com as espécies de C. krusei e C. albicans frente aos extratos brutos da folha e do fruto de A. muricata, com halo de 30mm e 22mm respectivamente. Em relação ao fracionamento químico realizado, os produtos obtidos a partir das frações butanólica e hexánica da folha sobre cepas de C. krusei, C. albicans e C. parapsilosis foram os que apresentaram resultados mais significativos. Dessa forma, comprovou-se que substancias presentes nesse vegetal podem ser utilizadas no controle cepas de Candida, contudo, estudos mais aprofundados são necessários para identificação e isolamento dos compostos biológicos ativos desses vegetais.

scholarly journals In Vitro Comparison of Terbinafine and Itraconazole against Penicillium marneffei

2000 ◽  
Vol 44 (5) ◽  
pp. 1407-1408 ◽  
Author(s):  
Michael R. McGinnis ◽  
Nicole G. Nordoff ◽  
Neil S. Ryder ◽  
Gary B. Nunn
Keyword(s):  
Clinical Laboratory ◽  
Geometric Mean ◽  
Penicillium Marneffei ◽  
National Committee ◽  
Geometric Mean Titer ◽  
Broth Macrodilution ◽  
Glucose Agar ◽  
Sabouraud Glucose Agar ◽  
Drug Dilution

ABSTRACT We evaluated terbinafine and itraconazole against 30 isolates ofPenicillium marneffei using a modification of the National Committee for Clinical Laboratory Standards broth macrodilution MIC testing protocol for yeasts. The minimal fungicidal concentration (MFC) was determined by plating 100 μl from each MIC drug dilution having no growth onto Sabouraud glucose agar incubated at 30°C. The MFC was the dilution at which growth was absent at 72 h of incubation. The MICs, in micrograms per milliliter, were as follows: terbinafine, 0.03 to 1.0 (geometric mean titer, 0.09); itraconazole, 0.03 to 0.5 (geometric mean titer, 0.04). The MFCs, in micrograms per milliliter, were as follows: terbinafine, 0.03 to 8 (geometric mean titer, 2.60); itraconazole, 0.03 to 8 (geometric mean titer, 2.45). Primary fungicidal activity (MFC within 2 dilutions of MIC) was observed with terbinafine in eight isolates and with itraconazole in four isolates. The data indicate that terbinafine is active against P. marneffei in vitro and may have a previously unrealized role in the management of infections caused by this fungus.

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