Real-time PCR for detection of Streptococcus suis serotype 2 in cerebrospinal fluid of human patients with meningitis

Introduction: Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that causes meningitis in China. This study’s aim was comparative analysis of serum proteomics from meningitis and non-meningitis piglets. Methodology: SS2 meningitis and non-meningitis piglet models were established. The serum samples were collected and analyzed by label-free LC-MS/MS proteomics technology. Differentially expressed proteins (DEPs) from serum were screened out by comparing the meningitis group and non-meningitis group to the healthy group (M/C; N/C), respectively. And then, globally and comparative analysis of DEPs in “M/C” and “N/C” in serum were performed using bioinformatics method. Finally, we comparatively analyzed the serum and cerebrospinal fluid proteomics in piglets that lived with meningitis. Results: We obtained 316 and 191 DEPs from “M/C” and “N/C” which classification visualizations were established. 157 DEPs were common in both groups and 159 DEPs were unique to the “M/C”. These DEPs and the signaling pathways which they participated in were visualized. Moreover, some DEPs which participated in multiple pathways were discovered and the interaction between 159 DEPs was also mapped. 39 common DEPs were also screened out in serum and cerebrospinal fluid during meningitis, and signaling pathways associated with these DEPs were further visualized. Conclusions: DEPs in “M/C” and “N/C” were comparatively analyzed and the similarities and differences of these DEPS which were involved in signal pathways were summarized. Moreover, several important molecules were screened out.

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Molecular diagnostics and ITS-based phylogenic analysis of Streptococcus suis serotype 2 in central Vietnam

The Journal of Infection in Developing Countries ◽

10.3855/jidc.7027 ◽

2015 ◽

Vol 9 (06) ◽

pp. 624-630 ◽

Cited By ~ 2

Author(s):

Bach Hoang Nguyen ◽

Dieu Hong Nu Phan ◽

Hien Xuan Nguyen ◽

An Van Le ◽

Alberto Alberti

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Pathogenic Bacteria ◽

Streptococcus Suis ◽

Its Sequences ◽

Pcr Assay ◽

Phylogenic Analysis ◽

Central Vietnam ◽

Suis Serotype ◽

Culture Dependent

Introduction: Streptococcus suis (S. suis) serotype 2 has recently become the most prevalent cause of meningitis in adults in many areas of Vietnam. This study provides data on S. suis molecular diagnosis in central Vietnam using a real-time polymerase chain reaction (PCR) assay targeting the S. suis serotype 2 cps2J gene. Additionally, 16S-23S rDNA intragenic spacer (ITS)-based phylogenic analysis of strains isolated from cerebrospinal fluid (CSF) in Thua Thien Hue Province, Vietnam, is presented and discussed. Methodology: Pathogenic bacteria were isolated from 40 CSF samples, and 18 were identified as S. suis by culture-dependent methods. Capsular serotyping was assessed by real-time PCR. ITS sequences were obtained after traditional PCR and were used in phylogenic analyses. Results: Pathogenic bacteria were isolated from 36 out of 40 CSF samples. A total of 18 S. suis strains were isolated and assigned to serotype 2 by real-time PCR. One CSF sample, negative when tested by culture-dependent methods, was positive to S. suis serotype 2 by real-time PCR. Pairwise alignments of the 18 ITS sequences did not reveal any variable nucleotide position, and resulted in a single sequence type. Sequences were similar to S. suis serotype 2 reference ITS sequences (> 98.1%), and there was no lack of an ITS spacer region in the isolates. Conclusions: S. suis serotype 2 is the most prevalent serotype in central Vietnam. Real-time PCR assay proved to be a reliable diagnostic method for early detection of S. suis 2 in CSF samples.

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DEVELOPMENT OF MULTIPLEX REAL-TIME PCR ASSAY FOR SIX PATHOGENS: RAPID TOOL FOR DIAGNOSIS OF BACTERIAL MENINGITIS

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2019.3.8 ◽

2019 ◽

pp. 60-66

Author(s):

Viet Quynh Tram Ngo ◽

Thi Ti Na Nguyen ◽

Hoang Bach Nguyen ◽

Thi Tuyet Ngoc Tran ◽

Thi Nam Lien Nguyen ◽

...

Keyword(s):

Bacterial Meningitis ◽

Real Time ◽

Real Time Pcr ◽

Diagnostic Methods ◽

Type B ◽

E Coli ◽

Pcr Assays ◽

Set Up ◽

Etiological Agents ◽

Suis Serotype

Introduction: Bacterial meningitis is an acute central nervous infection with high mortality or permanent neurological sequelae if remained undiagnosed. However, traditional diagnostic methods for bacterial meningitis pose challenge in prompt and precise identification of causative agents. Aims: The present study will therefore aim to set up in-house PCR assays for diagnosis of six pathogens causing the disease including H. influenzae type b, S. pneumoniae, N. meningitidis, S. suis serotype 2, E. coli and S. aureus. Methods: inhouse PCR assays for detecting six above-mentioned bacteria were optimized after specific pairs of primers and probes collected from the reliable literature resources and then were performed for cerebrospinal fluid (CSF) samples from patients with suspected meningitis in Hue Hospitals. Results: The set of four PCR assays was developed including a multiplex real-time PCR for S. suis serotype 2, H. influenzae type b and N. meningitides; three monoplex real-time PCRs for E. coli, S. aureus and S. pneumoniae. Application of the in-house PCRs for 116 CSF samples, the results indicated that 48 (39.7%) cases were positive with S. suis serotype 2; one case was positive with H. influenzae type b; 4 cases were positive with E. coli; pneumococcal meningitis were 19 (16.4%) cases, meningitis with S. aureus and N. meningitidis were not observed in any CSF samples in this study. Conclusion: our in-house real-time PCR assays are rapid, sensitive and specific tools for routine diagnosis to detect six mentioned above meningitis etiological agents. Key words: Bacterial meningitis, etiological agents, multiplex real-time PCR

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d-enantiomers of CATH-2 enhance the response of macrophages against Streptococcus suis serotype 2

Journal of Advanced Research ◽

10.1016/j.jare.2021.05.009 ◽

2021 ◽

Author(s):

Roel M. van Harten ◽

Johanna L.M. Tjeerdsma-van Bokhoven ◽

Astrid de Greeff ◽

Melanie D. Balhuizen ◽

Albert van Dijk ◽

...

Keyword(s):

Streptococcus Suis ◽

Suis Serotype ◽

Streptococcus Suis Serotype 2

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Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

AMB Express ◽

10.1186/s13568-020-01042-2 ◽

2020 ◽

Vol 10 (1) ◽

Cited By ~ 1

Author(s):

Quan Li ◽

Yang Fu ◽

Genglin Guo ◽

Zhuohao Wang ◽

Wei Zhang

Keyword(s):

Immunoglobulin G ◽

Binding Proteins ◽

Streptococcus Suis ◽

Human Immunoglobulin ◽

Human Immunoglobulin G ◽

Binding Regions ◽

Suis Serotype ◽

Streptococcus Suis Serotype 2

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Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum

Journal of Medical Microbiology ◽

10.1099/jmm.0.066142-0 ◽

2014 ◽

Vol 63 (2) ◽

pp. 309-312 ◽

Cited By ~ 16

Author(s):

Georg Härter ◽

Hagen Frickmann ◽

Sebastian Zenk ◽

Dominic Wichmann ◽

Bettina Ammann ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Real Time ◽

Real Time Pcr ◽

Clinical Symptoms ◽

Diagnostic Procedures ◽

Lower Limbs ◽

Antibody Specificity ◽

Quantitative Real Time Pcr ◽

Specific Antibodies ◽

Routine Diagnosis

We describe the case of a 16-year-old German male expatriate from Ghana who presented with obstipation, dysuria, dysaesthesia of the gluteal region and the lower limbs, bilateral plantar hypaesthesia and paraesthesia without pareses. A serum–cerebrospinal fluid (CSF) Schistosoma spp. specific antibody specificity index of 3.1 was considered highly suggestive of intrathecal synthesis of anti-Schistosoma spp. specific antibodies, although standardization of this procedure has not previously been described. Diagnosis was confirmed by detection of Schistosoma DNA in CSF by semi-quantitative real-time PCR at 100-fold concentration compared with serum. Accordingly the two diagnostic procedures, which have not previously been applied for routine diagnosis, appear to be useful for the diagnosis of neuroschistosomiasis. Clinical symptoms resolved following anthelmintic and anti-inflammatory therapy.

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