Evaluation of different phenotypic methods to detect methicillin resistance in Staphylococcus aureus isolates recovered from cystic fibrosis patients

2021 ◽  
pp. 115559
Author(s):  
Andrea García-Caballero ◽  
Juan de Dios Caballero ◽  
Ainhize Maruri ◽  
Maria Isabel Serrano-Tomás ◽  
Rosa del Campo ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Phenotypic Methods

scholarly journals IDENTIFICATION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS USING TOUCHDOWN PCR AND PHENOTYPIC METHODS FROM PATIENTS AND HOSPITALS ENVIRONMENTS IN DIFFERENT IRAQI CITIES

2021 ◽  
Vol 52 (6) ◽  
pp. 1356-1364
Author(s):  
A. M. Abd Zaid ◽  
N. J. Kandala
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Multidrug Resistant ◽  
Sensitivity Test ◽  
Methicillin Resistant ◽  
Cultural Methods ◽  
Base Medium ◽  
Touchdown Pcr ◽  
Phenotypic Methods

The study was aimed to evaluate the prevalence of MRSA in some Iraqi hospitals and determine the most powerful methods for identification of MRSA, in order to achieve the, 278 samples were collected from different hospitals in Iraq in various intervals, 204 out of 287 were identified as Staphylococcus aureus by conventional cultural methods and microscopic characteristics and 177 isolates are identified as MRSA by using HiCrome MeReSa Agar Base medium, but 154 of 177 (87%) isolates are methicillin resistance in sensitivity test. MRSA isolates were highly resistant to β-lactam antibiotics and considered multidrug resistant (MDR) in percent of (94.9%). Touchdown PCR used to identify the isolates, 97.05% were identified as Staphylococcus aureus, while 80.88%  as MRSA.                  

Methicillin resistance and vancomycin heteroresistance in Staphylococcus aureus in cystic fibrosis patients

2010 ◽  
Vol 29 (10) ◽  
pp. 1277-1285 ◽  
Author(s):  
V. Cafiso ◽  
T. Bertuccio ◽  
D. Spina ◽  
F. Campanile ◽  
D. Bongiorno ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Methicillin Resistance

scholarly journals DEVELOPMENT AND ANAYSIS OF A CYSTIC FIBROSIS SPECIFIC ANTIBIOGRAM

2021 ◽  
Author(s):  
E. Elson ◽  
Ellen Meier ◽  
Doug Swanson ◽  
Rangaraj Selvarangan ◽  
Megan Gripka ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Selection Methods ◽  
Antibiotic Selection ◽  
Gram Negative ◽  
Oropharyngeal Swab ◽  
Lung Infections ◽  
Over Time

BACKGROUND: Antibiotic therapy is essential for the treatment of cystic fibrosis (CF) lung infections. CF-specific airway pathophysiology and frequent antimicrobial exposure increase the risk of resistant infections, creating challenges to antibiotic selection. Antibiotic selection is generally based on previous cultures or hospital-wide antibiograms (HWA); however, most HWA exclude CF isolates. We developed a multi-year CF antibiogram (CFA) to compare with HWA and inform antibiotic selection. METHODS: CF culture data were collected 2015 - 2019 at a single pediatric CF center. All sputum and oropharyngeal swab isolates are included in the CFA. Demographics, microorganism isolates, and susceptibility information are presented. Susceptibilities were reported for methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (PA), Achromobacter species, Burkholderia species and Stenotrophomonas maltophilia. RESULTS: Over five years, the proportion of all SA isolates having methicillin-resistance was higher in the HWA (32%) than the CFA (28%). The most common gram-negative CF isolate was PA. Both gram-positive and gram-negative microorganisms were less susceptible in the CFA versus the HWA. CF isolates from sputum were less susceptible than oropharyngeal. MSSA and MRSA had significantly lower clindamycin susceptibility in the CFA compared to the HWA (MSSA 71% vs 79%, p<0.0001 and MRSA 39% vs 83%, p<0.0001). For every antimicrobial tested, PA isolates were less susceptible in the CFA compared to the HWA. There did not appear to be significant changes in susceptibility of CF isolates over time. CONCLUSIONS: These findings have clinical implications for empiric antimicrobial selection. A CFA will allow monitoring of resistance over time.

scholarly journals Comparison of five methods for oxacillin susceptibility testing of Staphylococcus aureus isolates from cystic fibrosis patients

2012 ◽  
Vol 54 (6) ◽  
pp. 305-306 ◽  
Author(s):  
Marcelo J. Mimica ◽  
Rozane L. Carvalho ◽  
Eitan N. Berezin ◽  
Neiva Damaceno ◽  
Hélio H. Caiaffa-Filho
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Sensitivity And Specificity ◽  
Gold Standard ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Susceptibility Testing ◽  
Meca Gene ◽  
Phenotypic Methods ◽  
Adequate Management ◽  
Sccmec Types

Methicillin-resistant Staphylococcus aureus (MRSA) are now a worldwide problem. Cystic fibrosis (CF) patients are commonly colonized and infected by MRSA. Accurate oxacillin susceptibility testing is mandatory for the adequate management of these patients. We performed a comparison of the accuracy of different tests in CF isolates, including methicillin-susceptible S. aureus and MRSA with different SCCmec types, and using the mecA gene as the gold-standard. The sensitivity and specificity of oxacillin disc, Etest, and oxacillin agar screening plate were 100%. Sensitivity of the cefoxitin disc was 85% and specificity was 100%. For clinically relevant isolates, laboratories may consider the use of a combination of two phenotypic methods.

scholarly journals Epidemiology ofStaphylococcus aureusin patients with cystic fibrosis

1994 ◽  
Vol 112 (3) ◽  
pp. 489-500 ◽  
Author(s):  
C. Branger ◽  
J. M. Fournier ◽  
J. Loulergue ◽  
A. Bouvet ◽  
Ph. Goullet ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Capsular Polysaccharide ◽  
Methicillin Resistance ◽  
Phage Type ◽  
Genotypic Diversity ◽  
Rational Approach ◽  
Capsular Type ◽  
Group Iii ◽  
Wide Range

SUMMARYSeven hundred and thirty-four isolates ofStaphylococcus aureus, recovered from the sputum of 238 cystic fibrosis patients in six French hospitals, were characterized by esterase electrophoretic typing, capsular polysaccharide serotyping and phage typing and tested against 14 antibiotics for sensitivity. Thirty-four esterase electrophoretic types were found with a genotypic diversity coefficient of 0·91. Five hundred and forty-eight (78·7%) isolates produced capsular polysaccharide and 350 (50·3%) were type 8. Four hundred and sixty isolates (66·6%) were phage typable and 202 (28·2%) were lysed by group III bacteriophages. No esterase electrophoretic type, capsular type or phage type was specific to cystic fibrosis. Isolates belonged to a wide range of types, similar to strains acquired outside hospitals. Eighty-five patients had three or more consecutive isolates over at least 6 months. The ability ofS. aureusto persist for long periods of time has been demonstrated in 73% of them. Methicillin-resistance was encountered among 73 strains (9·8%) which were also multiresistant. Two hundred and eighty-nine (39·9%) strains were sensitive to all antibiotics tested except to penicillin. Pristinamycin and co-trimoxazole were the most effective antibiotics. These results could contribute to the elaboration of a rational approach to the prophylaxis and therapy of respiratory staphylococcal infections in cystic fibrosis patients.

scholarly journals Detection of Methicillin Resistance in Staphylococcus Aureus by Polymerase Chain Reaction and Conventional Methods: A Comparative Study

2012 ◽  
Vol 4 (02) ◽  
pp. 083-088 ◽  
Author(s):  
Manju M Pillai ◽  
Ragunathan Latha ◽  
Gautam Sarkar
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Disk Diffusion ◽  
Chain Reaction ◽  
Mannitol Salt Agar ◽  
Polymerase Chain ◽  
Conventional Methods ◽  
Phenotypic Methods

ABSTRACT Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide, which has emerged over the past 30 years as a leading cause of both nosocomial and community-acquired infections. Accurate and rapid identification of MRSA in clinical specimens is essential for timely decision on effective antimicrobial chemotherapy. Aim: The present study was conducted to compare two conventional phenotypic methods, oxacillin disk diffusion (ODD) Aim: The present study was conducted to compare two conventional phenotypic methods, oxacillin disk diffusion (ODD) method and mannitol salt agar (MSA) with oxacillin, with polymerase chain reaction (PCR) for mecA gene (as standard). Materials and Methods: A total of 165 consecutive clinical isolates of S. aureus received at the Department of Microbiology in our tertiary care teaching hospital were included in the study. All the isolates were subjected to ODD (1 μg) method, culture in MSA with oxacillin, and PCR for mecA gene. Results: The sensitivity and specificity of ODD test were found to be 93.5% (86.4-97.3%) and 83.5% (79.2-85.8%), respectively, and that of MSA with oxacillin were found to be 87.1% (79.5-92.3%) and 89.3% (84.8-92.5%), respectively. The time taken for diagnosing MRSA by conventional methods is 48-72 h, which is more as compared to PCR which takes 18-24 h. Conclusion: This study recommends advocating PCR for mecA gene on a regular basis for detecting methicillin resistance in S. aureus isolates isolated from sterile body fluids or from special units such as intensive care units.

scholarly journals MOLECULAR CHARACTERIZATION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM CLINICAL CASES IN EAST ALGERIA

2016 ◽  
Vol 10 (1) ◽  
pp. 59
Author(s):  
Rahima Touaitia ◽  
Soumia Bektache ◽  
Nafissa Boutefnouchet ◽  
Abdelghani Djahoudi ◽  
Mohamed Bachtarzi
Keyword(s):  
Staphylococcus Aureus ◽  
Toxic Shock Syndrome ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Toxic Shock ◽  
Gene Encoding ◽  
Methicillin Resistant ◽  
Toxic Shock Syndrome Toxin ◽  
Phenotypic Methods

ABSTRACTObjective: The purpose of this study is to investigate the methicillin resistance gene, and some virulence factors in methicillin-resistant Staphylococcusaureus (MRSA) isolates by polymerase chain reaction (PCR).Methods: This study has included 12 MRSA isolates. All isolates were previously identified as S. aureus by a standard microbiological procedure, anda detection of methicillin resistance was realized by phenotypic methods. Following genomic DNA extraction, the presence of gyrA, mecA, lukPV, andtst genes was analyzed by duplex PCR. All retained S. aureus species have been found to contain gyrA gene.Results: Ten stains have been found to harbor mecA gene indicating it’s responsibility for methicillin resistance in those strains. Among the 12 strains,six of which were found to be Panton-Valentine leukocidine positive while none of which has tst gene encoding the toxic shock syndrome toxin.Conclusion: The pathogenesis of MRSA infections is related to the expression of a wide variety of virulence factors.Keywords: Methicillin-resistant Staphylococcus aureus, Panton-Valentine leukocidine, Toxic shock syndrome toxin 1, Multidrug resistance, mecA.

scholarly journals Distribution and Molecular Detection of Methicilin-Resistant Staphylococcus aureus

2021 ◽  
Author(s):  
Velma Rebić ◽  
Mufida Aljičević ◽  
Sajra Vinčević-Smajlović ◽  
Damir Rebić
Keyword(s):  
Staphylococcus Aureus ◽  
General Population ◽  
Bacterial Resistance ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Hospital Environment ◽  
Spa Typing ◽  
National Monitoring ◽  
Empiric Antibiotic ◽  
Phenotypic Methods

Isolation of Staphylococcus aureus is quite common in both the general population and hospital environment. The heterogeneity of the disease and the unique ability of S. aureus to develop resistance to the most recently discovered antibacterial drugs points to its ability to adapt and survive in different conditions. CA-MRSA is different from hospital strains of MRSA by its epidemiological, phenotypic and genotypic characteristics. The emergence of MRSA in the community suggests the need for a new approach to managing the indications and the certification of staphylococcal infections, with special emphasis on the selection of empiric antibiotic therapy. In the study, we analised of MRSA from 4341 samples taken from patients from the general population of Sarajevo Canton in the six-month period of follow-up processed at the Public Health Institute of Sarajevo Canton. We determined the epidemiological characteristics of the isolated strains. Methicillin resistance was determined by phenotypic methods. The following molecular methods were used for the confirmation of methicillin resistance: determination of the mecA gene, PFGE profile, genetic type of MRSA being determined by spa typing, the distribution of SCCmec types being examined, and the detected gene for PVL. The study stresses the need for national monitoring of spreading of the existing epidemic strains, as well as the monitoring of emergence of new strains which would enable the inclusion of our country in the international network of monitoring bacterial resistance.

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