scholarly journals Adsorption of cadmium and lead onto live and dead cell mass of Pseudomonas aeruginosa: A dataset

Data in Brief ◽  
2018 ◽  
Vol 18 ◽  
pp. 1185-1192 ◽  
Author(s):  
Mahnaz Karimpour ◽  
Seyed Davoud Ashrafi ◽  
Kamran Taghavi ◽  
Ali Mojtahedi ◽  
Esmaeil Roohbakhsh ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Mass ◽  
Dead Cell ◽  
Cadmium And Lead

Statistical Modelling and Optimisation of the Biosorption of Cd(II) and Pb(II) onto Dead Biomass of Pseudomonas Aeruginosa

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Joshua O. Ighalo ◽  
Adewale George Adeniyi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Metal Concentration ◽  
Cell Mass ◽  
Operating Conditions ◽  
Coefficient Of Determination ◽  
Aqueous Environment ◽  
Dead Cell ◽  
Operational Parameters ◽  
Dead Biomass ◽  
Agitation Time

AbstractDead biomass of micro-organisms can be used as biosorbents for the mitigation of heavy metal pollution in the aqueous environment. The aim of this study was to statistically model and optimise the sorption of Cd(II) and Pb(II) by dead biomass of Pseudomonas aeruginosa and to study the interactions between operating conditions. Statistically significant models were obtained for Cd(II) and Pb(II) sorption. The standard deviation for the Cd(II) and Pb(II) models were 0.86 and 1.54 while the coefficient of determination (R2) were 0.9978 and 0.9928 respectively. For both models, the adjusted R2 was in good agreement with the predicted R2 as the difference was less than 0.2. Numerical optimisation revealed that optimum Cd(II) removal of 88.6 % can be achieved at 1.172 ppm initial metal concentration, pH of 8.85, temperature of 43.72 °C, agitation time of 125.96 minutes and dead cell mass of 114.8 mg. Also, an optimum Pb(II) removal of 100 % can be achieved at 1.936 ppm initial metal concentration, pH of 6.88, temperature of 37.24 °C, agitation time of 130.57 minutes and dead cell mass of 122.85 mg. The study has revealed that at careful selected operational parameters, dead biomass of Pseudomonas aeruginosa can be valorised for the removal of heavy metals in aqueous media.

scholarly journals Cadmium and lead tolerant bacteria isolated from industrial waste water

2017 ◽  
Vol 26 (1) ◽  
pp. 29-38
Author(s):  
Md Arifur Rahman Bhuiyan ◽  
Tazeen Fatima Khan ◽  
Shahjahan Choudhury ◽  
SM Imamul Huq
Keyword(s):  
Waste Water ◽  
Heavy Metals ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Industrial Waste ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Industrial Waste Water ◽  
Acinetobacter Baumanii ◽  
Cadmium And Lead

An in vitro study was conducted to isolate, identify and characterize heavy metal resistant bacteria from industrial waste water and to determine their tolerance capacity to cadmium and lead. Different morphological, physiological and biochemical tests were carried out to identify the bacterial isolates in the waste water. A total of eight bacterial isolates viz., Staphyllococcus intermedius, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Escherichia coli, Acinetobacter baumanii, Pseudomonas flavescens and Acinetobacter lwofii were identified from metal polluted tannery and steel industrial areas. Bacterial response to cadmium tolerance was determined by treating them with CdCl2 solution at a rate of 1, 2, 2.5 and 5 μg/ml. In case of lead tolerant bacteria, PbCl2 solution was applied at a rate of 0.05, 0.125, 0.2 and 0.5 μg/ml. Pseudomonas aeruginosa isolated from waste water of steel industries and Bacillus cereus from tannery area was found to be the most tolerant species to the different doses of cadmium and lead. The study indicated that Pseudomonas aeruginosa and Bacillus cereus could be good candidates for the treatment and elimination of heavy metals from industrial waste water. The present study may be helpful to the bioremediation of heavy metals in the contaminated environment. Dhaka Univ. J. Biol. Sci. 26(1): 29-38, 2017 (January)

scholarly journals REMOVAL OF CADMIUM AND LEAD BY ADAPTED STRAINS OF Pseudomonas aeruginosa AND Enterobacter cloacae

2016 ◽  
Vol 32 (4) ◽  
pp. 407-412 ◽  
Author(s):  
Carolina Bojórquez ◽  
◽  
Martín Gabriel Frías Espericueta ◽  
Domenico Voltolina ◽  
◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enterobacter Cloacae ◽  
Cadmium And Lead

23 Biopsied invitro-produced bovine blastocysts survive vitrification better than slow freezing

2021 ◽  
Vol 33 (2) ◽  
pp. 119
Author(s):  
V. Najafzadeh ◽  
J. Secher ◽  
A. Andersen ◽  
N. Jørgensen ◽  
L. Strøbech ◽  
...  
Keyword(s):  
Inner Cell Mass ◽  
Cell Mass ◽  
Slow Freezing ◽  
Cell Counting ◽  
Expansion Rate ◽  
Tunel Staining ◽  
Dead Cell ◽  
Average Cell ◽  
Cell Numbers ◽  
Cell Counts

Trophectoderm (TE) biopsying for single nucleotide polymorphisms (SNPs) analysis is being implemented as a tool for the selection of elite bovine embryos. This biopsy method renders ample cells for analysis without compromising the inner cell mass (ICM), and the blastocyst recovers quickly after biopsy. To use the SNP data for embryo selection before the transfer, the blastocysts need to be cryopreserved either with vitrification or slow-freezing. In intact invitro-produced (IVP) blastocysts, vitrification has already proven optimal regarding embryo survival and pregnancy rates. This study aimed to investigate which cryopreservation approach is superior regarding blastocyst re-expansion rate as well as ICM, TE, and dead cell numbers after biopsying bovine IVP blastocysts. All IVP media and vitrification kits were from IVF Bioscience, and ethylene glycol with sucrose was from EggTech. Oocytes from slaughterhouse ovaries were used for blastocyst IVP. At Day 7, the blastocysts were pooled and randomised into 3 groups: (1) biopsy-control (BC), (2) biopsy-vitrification (BV), (3) biopsy-slow freeze (BSF). Subsequently, 5 to 10 TE cells were biopsied in BO-transfer medium using a 25-µm (inner diameter) biopsy pipette and flicking against the holding pipette. The BC group was incubated at 6% CO2 and 38.8°C for 5h. After scoring the re-expansion rate, the blastocysts were fixed with 4% paraformaldehyde/sucrose for further analyses. The BV and BSF groups were subjected to cryopreservation/thawing protocols according to the manufacturers’ instructions. Both groups, recovered under the same culture conditions as BC, were subsequently scored for re-expansion rate and finally fixed. For cell counting, the embryos were stained with Hoechst (DNA) and CDX2 (TE), combined with the TUNEL staining. ImageJ software (National Institutes for Health) was used for cell counting. P<0.05 was considered statistically significant and was determined using Fisher’s exact test for blastocyst re-expansion rate and Student’s t-test for cell numbers. The re-expansion rate in BV was 81% (61/75), which was significantly lower than in BC (95%; 225/236; P<0.005). In BSF, the re-expansion rate was 52% (28/54), which was significantly lower than in both BV and BC (both P<0.005). For cell counts, 18, 18, and 14 embryos, pooled from 3 to 4 independent IVP replicates, were analysed in BC, BV, and BSF, respectively. The average numbers of ICM cells in BV and BSF were 36±5 and 37±11, respectively, and neither was statistically different from BC (34±7; P>0.05). The average cell counts for TE cells in BV and BSF were 59±6 and 47±10, respectively, and neither was statistically different from BC (56±8; P>0.05). The average numbers of dead cells in BV and BSF were 8±3 and 9±2, respectively, and neither was statistically different from BC (10±4; P>0.05). In summary, the biopsied bovine IVP blastocysts recovered better after vitrification, and neither of cryopreservation methods had any effect on the numbers of ICM, TE, or dead cells. This project was supported by Innovation Fund Denmark and the Danish Milk Levy Foundation.

Use of F9 teratocarcinoma STEM cells to study cell-matrix interactions in the early mouse embryo

1992 ◽  
Vol 50 (1) ◽  
pp. 602-603
Author(s):  
Marc Lenburg ◽  
Rulang Jiang ◽  
Lengya Cheng ◽  
Laura Grabel
Keyword(s):  
Mouse Embryo ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Types ◽  
Embryoid Bodies ◽  
F9 Cells ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions ◽  
F9 Teratocarcinoma

We are interested in defining the cell-cell and cell-matrix interactions that help direct the differentiation of extraembryonic endoderm in the peri-implantation mouse embryo. At the blastocyst stage the mouse embryo consists of an outer layer of trophectoderm surrounding the fluid-filled blastocoel cavity and an eccentrically located inner cell mass. On the free surface of the inner cell mass, facing the blastocoel cavity, a layer of primitive endoderm forms. Primitive endoderm then generates two distinct cell types; parietal endoderm (PE) which migrates along the inner surface of the trophectoderm and secretes large amounts of basement membrane components as well as tissue-type plasminogen activator (tPA), and visceral endoderm (VE), a columnar epithelial layer characterized by tight junctions, microvilli, and the synthesis and secretion of α-fetoprotein. As these events occur after implantation, we have turned to the F9 teratocarcinoma system as an in vitro model for examining the differentiation of these cell types. When F9 cells are treated in monolayer with retinoic acid plus cyclic-AMP, they differentiate into PE. In contrast, when F9 cells are treated in suspension with retinoic acid, they form embryoid bodies (EBs) which consist of an outer layer of VE and an inner core of undifferentiated stem cells. In addition, we have established that when VE containing embryoid bodies are plated on a fibronectin coated substrate, PE migrates onto the matrix and this interaction is inhibited by RGDS as well as antibodies directed against the β1 integrin subunit. This transition is accompanied by a significant increase in the level of tPA in the PE cells. Thus, the outgrowth system provides a spatially appropriate model for studying the differentiation and migration of PE from a VE precursor.

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