We investigated whether regulation of VGLL4 by miR-222 affects proliferation, migration, invasion, and apoptosis in human tongue squamous cell carcinoma (TSCC) cells. Nanometer Magnetic beads were prepared to extract RNA in CAL27/human oral keratinocytes (HOK). Correlation between expression
of miR-222 and VGLL4 in CAL27/human oral keratinocytes (HOK) was analyzed using RT-PCR and western blot. The cellular effects of miR-222/VGLL4 interactions were detected by cck8, transwell, and cell scratch assays, and flow cytometry after miR-222 was silenced or overexpressed in CAL27 cells.
We assessed tumor growth in vivo, in a subcutaneous tumor model in mice, and assessed VGLL4 protein expression in tumors by immunohistochemistry. We found that miR-222 expression is higher in CAL27 cells than that in HOK cells. VGLL4 was highly expressed in HOK cells relative to CAL27
cells. When miR-222 was overexpressed, VGLL4 mRNA expression was reduced in CAL27 cells, and protein expression was reduced in the in vivo tumor model. Thus, miR-222 may down-regulate VGLL4 to promote proliferation, migration, and invasion of TSCC cells.
Data on the aquaporin gene expression differences among ρ0, clinically relevant radioresistant, and the parental cells of human cervical cancer and human tongue squamous cell carcinoma
