A new immortalized human liver sinusoidal endothelial cell line: Establishment, characterization, and application potential to development of in vitro human liver models

2017 ◽  
Vol 32 (1) ◽  
pp. S70
Author(s):  
Meiyan Zhu ◽  
Tomomi Furihata ◽  
Akira Koibuchi ◽  
Hideyuki Ide ◽  
Hanae Morio ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Human Liver ◽  
Sinusoidal Endothelial Cell ◽  
Liver Sinusoidal Endothelial Cell ◽  
Endothelial Cell Line ◽  
Cell Line Establishment ◽  
Application Potential

CONSTRUCTION OF AN IMMORTALIZED LIVER SINUSOIDAL ENDOTHELIAL CELL LINE FOR LIVER-TARGETED TISSUE ENGINEERING

ASAIO Journal ◽  
2001 ◽  
Vol 47 (2) ◽  
pp. 166
Author(s):  
T. Matsumura ◽  
N. Kobayashi ◽  
H. Noguchi ◽  
T. Totsugawa ◽  
T. Watanabe ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cell ◽  
Cell Line ◽  
Sinusoidal Endothelial Cell ◽  
Liver Sinusoidal Endothelial Cell ◽  
Endothelial Cell Line

scholarly journals EA-1, a novel adhesion molecule involved in the homing of progenitor T lymphocytes to the thymus.

1991 ◽  
Vol 114 (5) ◽  
pp. 1069-1078 ◽  
Author(s):  
B A Imhof ◽  
P Ruiz ◽  
B Hesse ◽  
R Palacios ◽  
D Dunon
Keyword(s):  
Endothelial Cell ◽  
T Cell ◽  
T Lymphocytes ◽  
Cell Line ◽  
Adhesion Molecule ◽  
Newborn Mice ◽  
Embryonic Mouse ◽  
Endothelial Cell Line ◽  
Mouse Tissues

The mouse progenitor T lymphocyte (pro-T) cell line FTF1 binds in vitro to thymus blood vessels, the thymic capsule, and liver from newborn mice. A mAb, EA-1, raised against an embryonic mouse endothelial cell line, blocked adhesion. The antibody also interfered with pro-T cell adhesion to a thymus-derived mouse endothelial cell line; it had no effect on the adhesion of mature T lymphocytes and myeloid cells. The antigen recognized by EA-1 is located on the vascular endothelium of various mouse tissues and absent on pro-T cells. EA-1 antibody precipitates molecules with apparent molecular weights of 110,000, 140,000, 160,000, and 200,000. Immunoclearing and binding-inhibition studies with antibodies against known adhesion molecules suggest that the EA-1 antigen is a novel adhesion molecule involved in colonization of the embryonic thymus by T cell progenitors.

Development of immortalized human cerebromicrovascular endothelial cell line as an in vitro model of the human blood–brain barrier

1997 ◽  
Vol 11 (13) ◽  
pp. 1187-1197 ◽  
Author(s):  
Arumugam Muruganandam ◽  
Leonie Moorhouse Herx ◽  
Robert Monette ◽  
Jon P. Durkin ◽  
Danica B. Stanimirovic
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Brain Barrier ◽  
Endothelial Cell Line ◽  
Blood Brain ◽  
Vitro Model

Effects of Phthalates on the Human Corneal Endothelial Cell Line B4G12

2012 ◽  
Vol 31 (4) ◽  
pp. 364-371 ◽  
Author(s):  
Tanja Krüger ◽  
Yi Cao ◽  
Søren K. Kjærgaard ◽  
Lisbeth E. Knudsen ◽  
Eva C. Bonefeld-Jørgensen
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Endothelial Cell ◽  
Cell Line ◽  
Corneal Endothelial Cell ◽  
Industrial Chemicals ◽  
Cell Secretion ◽  
Endothelial Cell Line ◽  
Butyl Phthalate

Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di- n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di- n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.

Effect of Long-term In Vitro Lithium Exposure on mRNA Levels of Claudin-3, CYP1A1, ABCG2 and GSTM3 Genes in the hCMEC/D3 Human Brain Endothelial Cell Line

2017 ◽  
Vol 42 (6) ◽  
pp. 1013-1017 ◽  
Author(s):  
Ramzi Shawahna ◽  
Kayathiri Ganeshamoorthy ◽  
Luo Huilong ◽  
Jean-Michel Scherrmann ◽  
Pierre-Olivier Couraud ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Human Brain ◽  
Cell Line ◽  
Mrna Levels ◽  
Brain Endothelial Cell ◽  
Endothelial Cell Line

HIF-1 activation attenuates postischemic myocardial injury: role for heme oxygenase-1 in modulating microvascular chemokine generation

2005 ◽  
Vol 289 (2) ◽  
pp. H542-H548 ◽  
Author(s):  
Ramzi Ockaili ◽  
Ramesh Natarajan ◽  
Fadi Salloum ◽  
Bernard J. Fisher ◽  
Drew Jones ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Heme Oxygenase ◽  
Ischemia Reperfusion ◽  
Microvascular Endothelial Cell ◽  
Endothelial Cell Line ◽  
Human Microvascular Endothelial Cell ◽  
Microvascular Endothelial

The CXC chemokine IL-8, which promotes adhesion, activation, and transmigration of polymorphonuclear neutrophils (PMN), has been associated with production of tissue injury in reperfused myocardium. Hypoxia-inducible factor-1 (HIF-1) is a heterodimeric peptide that is a key regulator of genes such as heme oxygenase (HO)-1 expressed under hypoxic conditions. We hypothesized that HO-1 plays an important role in regulating proinflammatory mediator production under conditions of ischemia-reperfusion. HIF-1 was activated in the human microvascular endothelial cell line (HMEC-1) with the prolyl hydroxylase inhibitor dimethyloxalylglycine (DMOG). DMOG significantly attenuated cytokine-induced IL-8 promoter activity and protein secretion and cytokine-induced PMN migration across human microvascular endothelial cell line HMEC-1 monolayers. In vivo studies in a rabbit model of myocardial ischemia-reperfusion showed that rabbits pretreated with a 20 mg/kg DMOG infusion ( n = 6) 24 h before study exhibited a 21.58 ± 1.76% infarct size compared with 35.25 ± 2.06% in saline-treated ischemia-reperfusion animals ( n = 6, change in reduction = 39%; P < 0.001). In DMOG-pretreated (20 mg/kg) animals, plasma IL-8 levels at 3 h after onset of reperfusion were 405 ± 40 pg/ml vs. 790 ± 40 pg/ml in saline-treated ischemia-reperfusion animals ( P < 0.001). DMOG pretreatment reduced myocardial myeloperoxidase activity, expressed as number of PMN per gram of myocardium, to 1.43 ± 0.59 vs. 4.86 ± 1.1 ( P = 0.012) in saline-treated ischemia-reperfused hearts. Both in vitro and in vivo DMOG-attenuated IL-8 production was associated with robust HO-1 expression. Thus our data show that HIF-1 activation induces substantial HO-1 expression that is associated with attenuated proinflammatory chemokine production by microvascular endothelium in vitro and in vivo.

ESTABLISHMENT OF AN IMMORTALIZED HUMAN-LIVER ENDOTHELIAL CELL LINE WITH SV40T AND hTERT

2004 ◽  
Vol 77 (9) ◽  
pp. 1357-1365 ◽  
Author(s):  
Toshihisa Matsumura ◽  
Michihiko Takesue ◽  
Karen A. Westerman ◽  
Teru Okitsu ◽  
Masakiyo Sakaguchi ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Human Liver ◽  
Endothelial Cell Line ◽  
Liver Endothelial Cell

Bioactive Cycloartane-Type Triterpene Glycosides from Astragalus elongatus

2008 ◽  
Vol 63 (11-12) ◽  
pp. 813-820 ◽  
Author(s):  
İhsan Çalış ◽  
Matej Barbič ◽  
Guido Jürgenliemk
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
2D Nmr ◽  
Microvascular Endothelial Cell ◽  
Inhibition Of Proliferation ◽  
Endothelial Cell Line ◽  
Human Microvascular Endothelial Cell ◽  
Esi Ms ◽  
Weak Activity

Abstract Together with two known cycloartane-type glycosides, askendosides D (3-O-[α-arabinopyranosyl-( 1→2)-β-xylopyranosyl]-6-O-β-xylopyranosyl-cycloastragenol, 2) and G (3-O- [α-arabinopyranosyl-(1→2)-β-xylopyranosyl]-16-O-β-glucopyranosyl-3β,6α,16β,24(R),25- pentahydroxycycloartane, 3), also a new monodesmosidic cycloartane-type glycoside, elongatoside (1), was isolated from the roots of Astragalus elongatus and identified as 3-O- [α-arabinopyranosyl-(1→2)-β-xylopyranosyl]-cycloastragenol. All structures were unambiguously determined by means of spectroscopic and spectrometric methods (1D and 2D NMR, ESI-MS). The isolated compounds were tested for the inhibition of proliferation and ICAM-1 expression in vitro using the human microvascular endothelial cell line (HMEC-1). 1 showed weak activity in the ICAM-1 assay.

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