ESTABLISHMENT OF AN IMMORTALIZED HUMAN-LIVER ENDOTHELIAL CELL LINE WITH SV40T AND hTERT

2004 ◽  
Vol 77 (9) ◽  
pp. 1357-1365 ◽  
Author(s):  
Toshihisa Matsumura ◽  
Michihiko Takesue ◽  
Karen A. Westerman ◽  
Teru Okitsu ◽  
Masakiyo Sakaguchi ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Human Liver ◽  
Endothelial Cell Line ◽  
Liver Endothelial Cell

scholarly journals Endothelial and Vascular Health: A Tale of Honey, H2O2 and Calcium

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1071
Author(s):  
Elia Ranzato ◽  
Gregorio Bonsignore ◽  
Mauro Patrone ◽  
Simona Martinotti
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Line ◽  
Cellular Responses ◽  
Pivotal Role ◽  
Vascular Health ◽  
Endothelial Cell Line ◽  
Positive Effects

Intracellular Ca2+ regulation plays a pivotal role in endothelial biology as well as during endothelial restoration processes. Interest in honey utilization in wound approaches is rising in recent years. In order to evaluate the positive effects of buckwheat honey on endothelial responses, we utilized an immortalized endothelial cell line to evaluate cellular responses upon honey exposure, with particular interest in Ca2+ signaling involvement. The results highlight the positive effects of buckwheat honey on endothelial cells’ responses and the central role played by Ca2+ signaling as an encouraging target for more efficacious clinical treatments.

Endothelin-1 production by a microvascular endothelial cell line treated with Plasmodium falciparum parasitized red blood cells

2002 ◽  
Vol 103 (s2002) ◽  
pp. 464S-466S ◽  
Author(s):  
Nicoletta BASILICO ◽  
Livianna SPECIALE ◽  
Silvia PARAPINI ◽  
Pasquale FERRANTE ◽  
Donatella TARAMELLI
Keyword(s):  
Plasmodium Falciparum ◽  
Endothelial Cell ◽  
Red Blood Cells ◽  
Cell Line ◽  
Blood Cells ◽  
Microvascular Endothelial Cell ◽  
Microvascular Endothelium ◽  
Endothelial Cell Line ◽  
Endothelin 1 ◽  
Microvascular Endothelial

In this study, we investigated the production of endothelin 1 (ET-1) by a human microvascular endothelial cell line, HMEC-1, co-cultured with Plasmodium falciparum-parasitized red blood cells (pRBCs). The results indicate that hypoxia increased the basal level of ET-1 production by HMEC-1 cells after 24 or 48h of treatment. However, the co-incubation of HMEC-1 cells with pRBCs, but not with uninfected RBCs, induced a dose-dependent decrease of both constitutive and hypoxia-induced ET-1 production. The inhibition was not due to a decrease in cell viability, as lactate dehydrogenase release remained constant. These results indicate that pRBCs are able to interfere with both the constitutive and stimulated ET-1 release from the microvascular endothelium, thus inducing local modifications of the vascular tone and of the inflammatory response. This could be of relevance in the pathogenesis of the most severe forms of P. falciparum infections, such as cerebral malaria or malaria during pregnancy.

scholarly journals RB459 and RB462 antibodies recognize mouse Pannexin1 protein by immunofluorescent staining

2019 ◽  
Vol 2 (2) ◽  
pp. e39
Author(s):  
Olga M Rusiecka ◽  
Christel L Roth ◽  
Brenda R Kwak ◽  
Filippo Molica
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Recombinant Antibodies ◽  
Immunofluorescent Staining ◽  
Mouse Tissue ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Endothelial Cell Line ◽  
Murine Macrophage Cell Line

The recombinant antibodies RB459 and RB462 recognize mouse Pannexin1 (Panx1) by immunofluorescent staining of a mouse cardiac endothelial cell line, a murine macrophage cell line and cryosections of mouse tissue.

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